scholarly journals Long-Term Effects of Magnetically Targeted Ferumoxide-Labeled Human Neural Stem Cells in Focal Cerebral Ischemia

2015 ◽  
Vol 24 (2) ◽  
pp. 183-190 ◽  
Author(s):  
Miyeoun Song ◽  
Young-Ju Kim ◽  
Yoon-Ha Kim ◽  
Jina Roh ◽  
Eun-Cheol Kim ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cerebral Ischemia ◽  
Neural Stem Cells ◽  
Focal Cerebral Ischemia ◽  
Long Term Effects ◽  
Human Neural Stem Cells

Using a Neodymium Magnet to Target Delivery of Ferumoxide-Labeled Human Neural Stem Cells in a Rat Model of Focal Cerebral Ischemia

2010 ◽  
Vol 21 (5) ◽  
pp. 603-610 ◽  
Author(s):  
Miyeoun Song ◽  
Young-Ju Kim ◽  
Yoon-ha Kim ◽  
Jina Roh ◽  
Seung U. Kim ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cerebral Ischemia ◽  
Neural Stem Cells ◽  
Rat Model ◽  
Focal Cerebral Ischemia ◽  
Target Delivery ◽  
Human Neural Stem Cells ◽  
Neodymium Magnet

Combined treatment of vascular endothelial growth factor and human neural stem cells in experimental focal cerebral ischemia

2005 ◽  
Vol 53 (4) ◽  
pp. 384-390 ◽  
Author(s):  
Kon Chu ◽  
Kyung-Il Park ◽  
Soon-Tae Lee ◽  
Keun-Hwa Jung ◽  
Song-Yi Ko ◽  
...  
Keyword(s):  
Stem Cells ◽  
Vascular Endothelial Growth Factor ◽  
Cerebral Ischemia ◽  
Growth Factor ◽  
Neural Stem Cells ◽  
Focal Cerebral Ischemia ◽  
Combined Treatment ◽  
Vascular Endothelial ◽  
Human Neural Stem Cells ◽  
Endothelial Growth Factor

scholarly journals Freshly Thawed Cryobanked Human Neural Stem Cells Engraft within Endogenous Neurogenic Niches and Restore Cognitive Function Following Chronic Traumatic Brain Injury

2020 ◽  
Author(s):  
Anna Badner ◽  
Emily K. Reinhardt ◽  
Theodore V. Nguyen ◽  
Nicole Midani ◽  
Andrew T. Marshall ◽  
...  
Keyword(s):  
Traumatic Brain Injury ◽  
Stem Cells ◽  
Brain Injury ◽  
Neural Stem Cells ◽  
Spatial Learning ◽  
Risk Taking ◽  
Mechanisms Of Action ◽  
Human Neural Stem Cells ◽  
Risk Taking Behavior

AbstractHuman neural stem cells (hNSCs) have potential as a cell therapy following traumatic brain injury (TBI). While various studies have demonstrated the efficacy of NSCs from on-going culture, there is a significant gap in our understanding of freshly thawed cells from cryobanked stocks – a more clinically-relevant source. To address these shortfalls, the therapeutic potential of our previously validated Shef-6.0 human embryonic stem cell (hESC)-derived hNSC line was tested following long-term cryostorage and thawing prior to transplant. Immunodeficient athymic nude rats received a moderate unilateral controlled cortical impact (CCI) injury. At 4-weeks post-injury, 6×105 freshly thawed hNSCs were transplanted into six injection sites (2 ipsi- and 4 contra-lateral) with 53.4% of cells surviving three months post-transplant. Interestingly, most hNSCs were engrafted in the meninges and the lining of lateral ventricles, associated with high CXCR4 expression and a chemotactic response to SDF1alpha (CXCL12). While some expressed markers of neuron, astrocyte, and oligodendrocyte lineages, the majority remained progenitors, identified through doublecortin expression (78.1%). Importantly, transplantation resulted in improved spatial learning and memory in Morris water maze navigation and reduced risk-taking behavior in an elevated plus maze. Investigating potential mechanisms of action, we identified an increase in ipsilateral host hippocampus cornu ammonis (CA) neuron survival, contralateral dentate gyrus (DG) volume and DG neural progenitor morphology as well as a reduction in neuroinflammation. Together, these findings validate the potential of hNSCs to restore function after TBI and demonstrate that long-term bio-banking of cells and thawing aliquots prior to use may be suitable for clinical deployment.Significance StatementThere is no cure for chronic traumatic brain injury (TBI). While human neural stem cells (hNSCs) offer a potential treatment, no one has demonstrated efficacy of thawed hNSCs from long-term cryobanked stocks. Frozen aliquots are critical for multisite clinical trials, as this omission impacted the use of MSCs for graft versus host disease. This is the first study to demonstrate the efficacy of thawed hNSCs, while also providing support for novel mechanisms of action – linking meningeal and ventricular engraftment to reduced neuroinflammation and improved hippocampal neurogenesis. Importantly, these changes also led to clinically relevant effects on spatial learning/memory and risk-taking behavior. Together, this new understanding of hNSCs lays a foundation for future work and improved opportunities for patient care.

scholarly journals Long-Term Survival of Human Neural Stem Cells in the Ischemic Rat Brain upon Transient Immunosuppression

PLoS ONE ◽  
2010 ◽  
Vol 5 (11) ◽  
pp. e14035 ◽  
Author(s):  
Laura Rota Nodari ◽  
Daniela Ferrari ◽  
Fabrizio Giani ◽  
Mario Bossi ◽  
Virginia Rodriguez-Menendez ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Rat Brain ◽  
Term Survival ◽  
Long Term Survival ◽  
Human Neural Stem Cells

scholarly journals Dose-dependent short- and long-term effects of ionizing irradiation on neural stem cells in murine hippocampal tissue cultures: neuroprotective potential of resveratrol

2016 ◽  
Vol 6 (10) ◽  
pp. e00548 ◽  
Author(s):  
Isabell Prager ◽  
Ina Patties ◽  
Katrin Himmelbach ◽  
Eva Kendzia ◽  
Felicitas Merz ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Tissue Cultures ◽  
Long Term Effects ◽  
Ionizing Irradiation ◽  
Hippocampal Tissue ◽  
Short And Long Term ◽  
Dose Dependent

scholarly journals Long-term Cultured Human Neural Stem Cells Undergo Spontaneous Transformation to Tumor-Initiating Cells

2011 ◽  
Vol 7 (6) ◽  
pp. 892-901 ◽  
Author(s):  
Weihua Wu ◽  
Qihua He ◽  
Xiaoxia Li ◽  
Xiaoyan Zhang ◽  
Aili Lu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Tumor Initiating Cells ◽  
Spontaneous Transformation ◽  
Human Neural Stem Cells

Folic acid stimulates proliferation of transplanted neural stem cells after focal cerebral ischemia in rats

2013 ◽  
Vol 24 (11) ◽  
pp. 1817-1822 ◽  
Author(s):  
Huan Liu ◽  
Jiasong Cao ◽  
Haihong Zhang ◽  
Shanchun Qin ◽  
Min Yu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Folic Acid ◽  
Cerebral Ischemia ◽  
Neural Stem Cells ◽  
Focal Cerebral Ischemia

Diazepam treatment blocks the elevation of hippocampal activity and the accelerated proliferation of hippocampal neural stem cells after focal cerebral ischemia in mice

2013 ◽  
Vol 91 (11) ◽  
pp. 1429-1439 ◽  
Author(s):  
Rokuya Nochi ◽  
Jun Kaneko ◽  
Natsumi Okada ◽  
Yasushi Terazono ◽  
Ayumu Matani ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cerebral Ischemia ◽  
Neural Stem Cells ◽  
Focal Cerebral Ischemia ◽  
Hippocampal Activity ◽  
Accelerated Proliferation ◽  
Hippocampal Neural Stem Cells
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