Development and Validation of Analytical Method for Simultaneous Estimation of Diclofenac Sodium and Ofloxacin in Bulk and Ophthalmic Formulations using UV–Visible Spectrometry

2011 ◽  
Vol 4 (2) ◽  
pp. 1399-1402
Author(s):  
Chawla P ◽  
Kumar S ◽  
Sahni V ◽  
Mamman K ◽  
S A Saraf
Keyword(s):  
Diclofenac Sodium ◽  
Simultaneous Equation ◽  
Simultaneous Equations ◽  
Simultaneous Estimation ◽  
Analysis Method ◽  
Q Value ◽  
Value Analysis ◽  
Uv Visible ◽  
Development And Validation ◽  
Validation Of Analytical Method

Two simple, accurate, precise, reproducible, requiring no prior separation and economical procedures for simultaneous estimation of diclofenac sodium and ofloxacin in bulk and ophthalmic formulations have been developed. The first method employs formation and solving of simultaneous equations using 273 nm and 291 nm as two analytical wavelengths for both drugs in acidic methanol. The second method is the Q value analysis based on measurements of absorptivity at 263 nm (as an iso-absorptive point) and 273 nm. Diclofenac sodium and ofloxacin at their respective λmax 273 nm and 291 nm and at isoabsorptive point 263 nm show linearity in a concentration range of 1-20 µg/ml. Recovery studies range from 99.67% for diclofenac sodium and 100.35% for ofloxacin in case of simultaneous equation method and 99.99% for diclofenac sodium and 100.07% for ofloxacin in the case of Q - analysis method confirming the accuracy of the proposed method. In conclusion, the proposed method is recommended for routine analysis since it is rapid, simple, accurate and also sensitive and specific

scholarly journals Simultaneous Estimation of Domperidone and Pantoprazole in Solid Dosage Form by UV Spectrophotometry

2006 ◽  
Vol 3 (3) ◽  
pp. 142-145
Author(s):  
P. Ravi Kumar ◽  
P. Bhanu Prakash ◽  
M. Murali Krishna ◽  
M. Santha Yadav ◽  
C. Asha Deepthi
Keyword(s):  
Simultaneous Equation ◽  
Simultaneous Equations ◽  
Simultaneous Estimation ◽  
Uv Spectrophotometry ◽  
Q Value ◽  
Value Analysis ◽  
Spectrophotometric Methods ◽  
Solid Dosage ◽  
Tablet Dosage

Domperidone is an antiemetic and pantoprazole is an antiulcer drug. Simple, precise, rapid and selective simultaneous equation and Q- analysis UV spectrophotometric methods have been developed for the simultaneous determination of domperidone and pantoprazole from combined tablet dosage forms. The methods involve solving of simultaneous equations and Q-value analysis based on measurement absorptivity at 216, 287 and 290 nm respectively. Linearity lies between 1-15 mcg/mL for domperidone and 0-50 mcg/mL for pantoprazole.

scholarly journals Development and validation of analytical method for Irbesartan and Atorvastatin by simultaneous equation spectroscopic method

2015 ◽  
Vol 1 (4) ◽  
pp. 194
Author(s):  
Paras B Virani ◽  
Rajanit Sojitra ◽  
Hasumati Raj ◽  
Vineet Jain
Keyword(s):  
Spectroscopic Method ◽  
Simultaneous Equation ◽  
Simultaneous Estimation ◽  
Limit Of Quantification ◽  
Limit Of Determination ◽  
Relative Standard ◽  
Acceptance Range ◽  
High Concentrations ◽  
Development And Validation ◽  
Validation Of Analytical Method

A simple, accurate and precise spectroscopic method was developed for simultaneous estimation of Irbesartan and atorvastatin in synthetic mixture using simultaneous equation Method. In this spectroscopic method, 226.00 nm and 246.00 nm wavelengths were selected for measurement of absorptivity. Both the drugs show linearity in a concentration range of 05-30 ?g/ml at their respective ?max. Accuracy, precision and recovery studies were done by QC samples covering lower, medium and high concentrations of the linearity range. The relative standard deviation for accuracy, precision studies were found to be within the acceptance range (<2%). The limit of determination was 0.033?g/ml and 0.125 ?g/ml for Irbesartan and atorvastatin, respectively. The limit of quantification was 0.1008 ?g/ml and 0.3792 ?g/ml for Irbesartan and atorvastatin, respectively. Recovery of Irbesartan and atorvastatin were found to be 99.75 % and 99.52% respectively confirming the accuracy of the proposed method. The proposed method is recommended for routine analysis since they are rapid, simple, accurate and also sensitive and specific by no heating and no organic solvent extraction.

SIMULTANEOUS ANALYSIS OF A THREE COMPONENT MIXTURE BY VIERODT’S SIMULTANEOUS EQUATION METHOD

INDIAN DRUGS ◽  
2016 ◽  
Vol 53 (10) ◽  
pp. 40-42
Author(s):  
A Palekar ◽  
◽  
S. Gaude ◽  
S. P. N. Pai
Keyword(s):  
Spectrophotometric Method ◽  
Dosage Form ◽  
Drug Product ◽  
Simultaneous Equation ◽  
Simultaneous Equations ◽  
Simultaneous Estimation ◽  
Component Mixture ◽  
Percent Recovery ◽  
And Performance ◽  
Tablet Dosage

A simple, rapid, sensitive and economical UV spectrophotometric method was developed and validated for the simultaneous estimation of aspirin, caffeine, and orphenadrine citrate by Vierodt’s method in combined tablet dosage form and using mixture of 0.1 N HCl: ethanol (1:1) as solvent. The method employs designing and solving of simultaneous equations using 3 wavelengths, namely 226 nm, 272 nm, and 210 nm. Beer’s law was obeyed in the concentration range of 3-18 μg/mL for aspirin and 0.5-12 μg/mL for both caffeine and orphenadrine citrate with r2 value of 0.993, 0.991, and 0.991 respectively. Accuracy was determined by recovery studies and showed percent recovery ranging from 99.78-100% for aspirin, 96.10-100.92% for caffeine and 100.53-102.5% for orphenadrine citrate. The developed method can be used in QC laboratory for routine analysis to ensure the identity, purity, and performance of the drug product.

scholarly journals Development and validation of UV spectroscopic methods for simultaneous estimation of ciprofloxacin and tinidazole in tablet formulation

2012 ◽  
Vol 1 (10) ◽  
pp. 317-321 ◽  
Author(s):  
Sowjanya Gummadi ◽  
Devi Thota ◽  
Sri Valli Varri ◽  
Pratyusha Vaddi ◽  
Venkata Lakshmi Narasimha Seshagiri Rao
Keyword(s):  
Phosphate Buffer ◽  
Dosage Form ◽  
Pharmaceutical Journal ◽  
Simultaneous Equations ◽  
Simultaneous Estimation ◽  
Spectroscopic Methods ◽  
Good Reproducibility ◽  
Statistical Validation ◽  
Development And Validation ◽  
Tablet Dosage

Two simple, accurate, precise, reproducible and economical UV spectroscopic methods (A &  B) for simultaneous estimation of Ciprofloxacin and Tinidazole in tablet dosage form have been developed. Method A employs solving of simultaneous equations based on the measurement of absorbance at two wavelengths, 271nm and 318nm which are the ?max values of Ciprofloxacin and Tinidazole respectively in phosphate buffer (pH 6.8). Method B is based on the principle of  Q-Analysis where in the absorbance was measured at 292nm (iso-absorptive point) and 271nm (?max of Ciprofloxacin)in phosphate buffer (pH 6.8). Ciprofloxacin and Tinidazole  shows linearity at all the selected wave-lengths and  obeys Beer’s law in the concentration range of 10-35µg/mL and 10-80µg/mL respectively.  Recovery studies for Ciprofloxacin and Tinidazole were performed and the percentage recovery for both the drugs was obtained in the range of 98.1-99.7% (Method A) and 98.0-100.4% (Method B) confirming the accuracy of the proposed method. Both the methods showed good reproducibility and recovery with %RSD less than 2. Statistical validation of the data shows that the proposed methods can be successfully applied for the routine analysis of drugs in commercial tablets.DOI: http://dx.doi.org/10.3329/icpj.v1i10.11849 International Current Pharmaceutical Journal 2012, 1(10): 317-321 

scholarly journals DEVELOPMENT AND VALIDATION OF SPECTROSCOPIC METHODS FOR SIMULTANEOUS ESTIMATION AND DISSOLUTION OF OFLOXACIN AND ORNIDAZOLE IN TABLET DOSAGE FORMS

2018 ◽  
Vol 35 (3) ◽  
pp. 123
Author(s):  
R. C. Mashru ◽  
S V. Saikumar
Keyword(s):  
Dosage Forms ◽  
Simultaneous Equations ◽  
Simultaneous Estimation ◽  
Spectroscopic Methods ◽  
Dissolution Studies ◽  
Significant Difference ◽  
Development And Validation ◽  
Tablet Dosage ◽  
Regulatory Filing ◽  
Paddle Apparatus

The aim of this work was to develop and validate simple, accurate and precise spectroscopic methods (multicomponent, dual wavelength and simultaneous equations) for the simultaneous estimation and dissolution testing of ofloxacin and ornidazole tablet dosage forms. The medium of dissolution used was 900 ml of 0.01N HCl, using a paddle apparatus at a stirring rate of 50 rpm. The drug release was evaluated by developed and validated spectroscopic methods. Ofloxacin and ornidazole showed 293.4 and 319.6nm as λ max in 0.01N HCl. The methods were validated to meet requirements for a global regulatory filing. The validation included linearity, precision and accuracy. In addition, recovery studies and dissolution studies of three different tablets were compared and the results obtained show no significant difference among products.

scholarly journals Development and Validation of Novel Analytical Simultaneous Estimation Based UV Spectrophotometric Method for Doxycycline and Levofloxacin Determination

2021 ◽  
Vol 12 (4) ◽  
pp. 5458-5478
Keyword(s):  
Solvent System ◽  
Quality Analysis ◽  
Simultaneous Estimation ◽  
Literature Study ◽  
Drug Products ◽  
Ich Guidelines ◽  
Percent Recovery ◽  
Uv Visible ◽  
Development And Validation

The thorough literature study uncovered that none of the most perceived pharmacopeias or any journals includes a method for simultaneous estimation of Doxycycline and Levofloxacin in combination by UV/Visible spectroscopy. So, it was felt fundamental to build up a system that will serve as a solid, precise UV technique for the simultaneous estimation of Doxycycline and Levofloxacin. DOXH and LVXH showed λmax at 273nm and 287nm respectively, and iso-absorptive point at 280nm in Phosphate buffer pH 6.8 prepared in Water: Methanol (80:20) dissolvable solvent system. Beer Lambert's law obeyed by both drugs within the concentration range of 2-20 μg/ml & r2 values of 0.9999 and 0.9998, which shows the good linearity. The method has been validated statistically and quantitatively regarding linearity, precision, LOD, LOQ, accuracy, and specificity according to the ICH guidelines. LOD for DOXH and LVXH were found to be 1.41 and 0.63 μg/ml, the LOQ was 4.30 and 1.92 μg/ml, respectively. Percent recovery at recovery level of 80%, 100% & 120% for DOXH was found to be 99.7, 99.66 & 99.69 & for LVXH 99.58, 99.66 & 99.63 respectively. Intra-day, Inter-day & precision analysis by different analyst was found to be 0.767, 0.563, 0.440 %RSD for DOXH & 0.507, 0.532, 0.708 % RSD for LVXH. Sandell's sensitivity was discovered to be adequate, and this shows that extremely less measure of the two medications can be successfully recognized by this technique. Finally, it was concluded, the developed & validated method was helpful and appropriate for regular quality analysis and simultaneous determination of drug products containing DOXH and LVXH in combination.

DEVELOPMENT AND VALIDATION OF A SENSITIVE SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS ESTIMATION OF PITAVASTATIN CALCIUM AND FENOFIBRATE

INDIAN DRUGS ◽  
2015 ◽  
Vol 52 (08) ◽  
pp. 17-21
Author(s):  
A Kukrety ◽  
◽  
K. Kohli ◽  
M. Singhal ◽  
C Dhal ◽  
...  
Keyword(s):  
Dosage Form ◽  
Spectroscopic Method ◽  
Simultaneous Equation ◽  
Simultaneous Estimation ◽  
Pharmaceutical Dosage Form ◽  
Standard Additions ◽  
Pitavastatin Calcium ◽  
Development And Validation ◽  
Different Levels ◽  
Sensitive Spectrophotometric Method

A simple, rapid, accurate, precise and reproducible UV spectroscopic method has been developed for the simultaneous estimation of pitavastatin calcium and fenofibrate in bulk and pharmaceutical dosage form. The method is based on simultaneous equation method. Pitavastatin calcium and fenofibrate have absorption maxima (λmax) at 245 nm and 286 nm respectively. Beer’s law was obeyed in the concentration range of 2-12 μg/mL and 32-192 μg/mL for pitavastatin calcium and fenofibrate, respectively. The recovery studies are indicative of accuracy of method and are found in between 97.66-107.22% and 103.33-110.55% for pitavastatin calcium and fenofibrate, respectively, at three different levels of standard additions. Precision studies showed satisfactory results.

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