scholarly journals Chemical Characterization of Artemisia Annua L. Extract Assessment of Antioxidant Activity in vitro and in vivo Toxicity Studies

2019 ◽  
Vol 70 (6) ◽  
pp. 1903-1907
Author(s):  
Roxana Liana Stan ◽  
Bogdan Sevastre ◽  
Adriana Corina Hangan ◽  
Sanda Bota ◽  
Daniela Hanganu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Artemisia Annua ◽  
Histopathological Examination ◽  
Haematological Toxicity ◽  
Chemical Characterization ◽  
In Vivo Toxicity ◽  
Artemisia Annua L ◽  
Toxicity Studies

The purpose of the study was to demonstrate in vitro antioxidant activity and in vivo toxicity of the Artemisia Annua L. extract. The plant was harvested from Bihor area (Crisul Repede and Negru river valleys), Romania. Preparation of the plant product and of the lyophilizated extract was carried out in accordance with the Romanian Pharmacopeia Xth Edition. Lyophilized extract was evaluated in terms of polyphenol content using HPLC method. Antioxidant activity was highlighted using the DPPH, ABTS and FRAP methods. Hepatic, renal and haematological toxicity studies have been performed on laboratory mice. For this purpose blood and organs were collected. Biochemical and haematological parameters were determined on the blood samples and histopathological examination was performed on organs. In vitro antioxidant effect of Artemisia Annua L extract and its lack of in vivo toxicity were demonstrated. It is desirable to obtain a new phytoproduct harvested from spontaneous flora of Romania with antioxidant / antitumoral properties and which is devoid of toxicity.

scholarly journals Toxoplasma gondii: Effects of Artemisia annua L. on susceptibility to infection in experimental models in vitro and in vivo

2009 ◽  
Vol 122 (3) ◽  
pp. 233-241 ◽  
Author(s):  
Taísa Carrijo de Oliveira ◽  
Deise A. Oliveira Silva ◽  
Cristina Rostkowska ◽  
Samantha Ribeiro Béla ◽  
Eloisa A.V. Ferro ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Artemisia Annua ◽  
Experimental Models ◽  
Susceptibility To Infection ◽  
Artemisia Annua L

scholarly journals Antioxidant activity and physico-chemical analysis of Campomanesia rufa (O.Berg) Nied. fruits

2020 ◽  
Vol 44 ◽  
Author(s):  
Letícia Aparecida Ferreira de Abreu ◽  
Renato Paiva ◽  
Judith Georgette Alcalde Mosqueira ◽  
Michele Valquíria dos Reis ◽  
Ana Beatriz Silva Araújo ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ph Value ◽  
Scientific Information ◽  
Chemical Characterization ◽  
High Water ◽  
Biological Properties ◽  
Soluble Solids ◽  
High Water Content

ABSTRACT Campomanesia rufa (O. Berg) Nied. is a native Cerrado species that presents great edible potential. However, it is a species “in danger of extinction” as recommended by the International Union for the Conservation of Nature (IUCN). No technical and scientific information about the species exists, thus demonstrating the importance of its research. The present work aimed at the physical and chemical characterization of immature and mature C. rufa fruits. The fruits showed a change in coloration from green (b * = 25.11, h = 122.43) to yellowish-green (b * = 34.26 , h = 115.73), an increase in mass (6.54 g to 10.88 g), diameter (23.76 mm to 28.03 mm) and soluble solids (8.00 to 10.80%). The fruits presented high levels of total (1246.35 mg 100 g-1) and soluble pectin (195.93 mg 100 g-1), high water content (78.86 g 100 g-1), low pH value (3.40), and high citric acid content (1.2%). However, the fruits had low protein (0.81 g 100 g-1), lipid contents, and low caloric values (64.76 kcal 100 g-1). The fruits presented significant values of carotenoids, phenolic compounds (312.47 mg 100 g-1), vitamin C (263.60 mg 100 g-1) as well as good in vitro antioxidant activity (1862.81 µM g-1). The results obtained indicate that C. rufa fruits showed a similar composition to the fruits of other Campomanesia species, and their biological properties should be investigated additionally under in vivo conditions.

scholarly journals Phytochemical composition, antifungal activity, in vitro and in vivo toxicity of Syzygium cumini (L.) Skeels leaves extract

2021 ◽  
Vol 20 (5) ◽  
pp. 536-555
Author(s):  
Ernani Canuto Figueiredo Junior ◽  
◽  
Yuri Wanderley Cavalcanti ◽  
Andressa Brito Lira ◽  
Hilzeth de Luna Freire Pessoa ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antifungal Activity ◽  
Galleria Mellonella ◽  
In Vitro Assays ◽  
Syzygium Cumini ◽  
Candida Spp ◽  
In Vivo Toxicity ◽  
Phytochemical Composition

This study determined phytochemical composition, antifungal activity and toxicity in vitro and in vivo of Syzygium cumini leaves extract (Sc). Thus, was characterized by gas chromatography coupled to mass spectrometry and submitted to determination of Minimum Inhibitory (MIC) and Fungicidal concentrations (MFC) on reference and clinical strains of Candida spp. and by growth kinetics assays. Toxicity was verified using in vitro assays of hemolysis, osmotic fragility, oxidant and antioxidant activity in human erythrocytes and by in vivo acute systemic toxicity in Galleria mellonella larvae. Fourteen different compounds were identified in Sc, which showed antifungal activity (MIC between 31.25-125 μg/mL) with fungistatic effect on Candida. At antifungal concentrations, it demonstrated low cytotoxicity, antioxidant activity and neglible in vivo toxicity. Thus, Sc demonstrated a promising antifungal potential, with low toxicity, indicating that this extract can be a safe and effective alternative antifungal agent.

In vitro and in vivo toxicity studies of copper sulfide nanoplates for potential photothermal applications

2015 ◽  
Vol 11 (4) ◽  
pp. 901-912 ◽  
Author(s):  
Wei Feng ◽  
Wei Nie ◽  
Yanhua Cheng ◽  
Xiaojun Zhou ◽  
Liang Chen ◽  
...  
Keyword(s):  
Copper Sulfide ◽  
In Vivo Toxicity ◽  
Toxicity Studies

Biologically Synthesized Plant-Derived Nanomedicines and Their In vitro-- In vivo Toxicity Studies in Various Cancer Therapeutics: Regulatory Perspectives

2021 ◽  
pp. 217-260
Author(s):  
Mohamed Sheik Tharik Abdul Azeeze ◽  
Santhosh Shanthi Bhupathi ◽  
Elmutaz Belah Mohammad ◽  
Durairaj Kaliannan ◽  
Balamuralikrishnan Balasubramanian ◽  
...  
Keyword(s):  
Cancer Therapeutics ◽  
In Vivo Toxicity ◽  
Toxicity Studies

In Vivo Toxicity Profile of NN-32 and Nanogold Conjugated GNP-NN-32 from Indian Spectacled Cobra Venom

2020 ◽  
Vol 21 (14) ◽  
pp. 1479-1488
Author(s):  
Saurabh S. Attarde ◽  
Sangeeta V. Pandit
Keyword(s):  
Chronic Toxicity ◽  
Significant Inhibition ◽  
Toxicity Profile ◽  
Naja Naja ◽  
In Vivo Toxicity ◽  
Toxicity Studies ◽  
Serum Biochemical ◽  
Reduced Toxicity

Background: NN-32 toxin, which was obtained from Naja naja venom and showed cytotoxicity on cancer cell lines. As the toxicity of NN-32 is the main hurdle in the process of drug development; hence, we have conjugated NN-32 toxin with gold nanoparticles (GNP-NN-32) in order to decrease the toxicity of NN-32 without reducing its efficacy, GNP-NN-32 alleviated the toxicity of NN-32 in in vitro studies during the course of earlier studies. In continuation, we are evaluating in vivo toxicity profile of NN-32 and GNP-NN-32 in the present study. Objective: To study in vivo toxicity profile of NN-32 and nanogold conjugated GNP-NN-32 from Naja naja venom. Materials and Methods: We have carried out in vivo acute toxicity study to determine LD50 dose of GNP-NN-32, in vivo sub-chronic toxicity for 30 days, haematology, serum biochemical parameters and histopathology study on various mice tissues and in vitro cellular and tissue toxicity studies. Results: The LD50 dose of GNP-NN-32 was found to be 2.58 mg/kg (i.p.) in Swiss male albino mice. In vivo sub-chronic toxicity showed significantly reduced toxicity of GNP-NN-32 as compared to NN-32 alone. Discussion: In vitro cellular toxicity studies on human lymphocyte and mouse peritoneal macrophage showed significant inhibition of cells by NN-32 alone. Conclusion: Conjugated GNP-NN-32 toxin showed less in vivo toxicity as compared to pure NN-32.

Are in vitro tests predictive of QT prolongation in human as in vivo toxicity studies are? The example of WIN33377

1998 ◽  
Vol 95 ◽  
pp. 192-193
Author(s):  
P. Gautier ◽  
A. Roberts ◽  
J.P. Bertrand ◽  
A. Barbier ◽  
J. Popp ◽  
...  
Keyword(s):  
Qt Prolongation ◽  
In Vitro Tests ◽  
In Vivo Toxicity ◽  
Toxicity Studies

scholarly journals In Vivo Toxicity and In Vitro Solubility Assessment of Pre-Treated Struvite as a Potential Alternative Phosphorus Source in Animal Feed

Animals ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 785 ◽  
Author(s):  
Soomin Shim ◽  
Seunggun Won ◽  
Arif Reza ◽  
Seungsoo Kim ◽  
Sungil Ahn ◽  
...  
Keyword(s):  
Animal Feed ◽  
Histopathological Examination ◽  
Sprague Dawley ◽  
Alternative Source ◽  
Lung Weight ◽  
In Vivo Toxicity ◽  
Significant Difference ◽  
Potential Alternative

Apart from using as fertilizer for plants, the application of struvite may be expanded to animal feed industries through proper pre-treatment. This study aimed to investigate the safety and efficacy of using pre-treated struvite (microwave irradiated struvite (MS) and incinerated struvite (IS)) in animal feeds. For safety assessment, an in vivo toxicity experiment using thirty female Sprague Dawley rats (average body weight (BW) of 200 ± 10 g) was conducted. The rats were randomly divided into five groups, including a control. Based on the BW, MS and IS were applied daily by oral administration with 1 and 10 mg kg−1-BW (MS1 and MS10; IS1 and IS10) using dimethyl sulfoxide (DMSO) as a vehicle. A series of jar tests were conducted for four hours to check the solubility of the MS and IS at different pH (pH 2, 4, 5, 6, and 7) and compared to a commercial P source (monocalcium phosphate, MCP, control). The toxicity experiment results showed no significant differences among the treatments in BW and organ (liver, kidney, heart, and lung) weight of rats (p > 0.05). There were no adverse effects on blood parameters and the histopathological examination showed no inflammation in the organ tissues in MS and IS treated groups compared to the control. In an in vitro solubility test, no significant difference was observed in ortho-phosphate (O-P) solubility from the MCP and MS at pH 2 and 4 (p > 0.05), while O-P solubility from MS at pH 5 to 7 was higher than MCP and found to be significantly different (p < 0.05). O-P solubility from IS was the lowest among the treatments and significantly different from MCP and MS in all the experiments (p < 0.05). The results of this study not only suggest that the struvite pre-treated as MS could be a potential alternative source of P in animal feed but also motivate further studies with more stringent designs to better examine the potential of struvite application in diverse fields.

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