scholarly journals Comparative Analysis of Parasitological, Immunodiagnostic and Histopathological Methods in the Diagnosis of Human Giardiasis

2020 ◽  
Vol 71 (1) ◽  
pp. 378-383
Author(s):  
Natasa Miladinovic Tasic ◽  
Vuka Katic
Keyword(s):  
Digestive Tract ◽  
Giardia Lamblia ◽  
Age Groups ◽  
Socioeconomic Conditions ◽  
Concentration Technique ◽  
High Prevalence ◽  
Stool Samples ◽  
Conventional Microscopy ◽  
Human Digestive Tract ◽  
Southeastern Serbia

Giardiasis/lambliosis is a disease caused by the presence of a protozoan Giardia lamblia (G. lamblia) in the upper portions of the human digestive tract (DT). This cosmopolitan parasite is present particularly in children, but in all other age groups and socioeconomic conditions as well. The studies undertaken in the Ni�ava District (southeastern Serbia) have demonstrated a high prevalence of infection caused by G. lamblia species using conventional microscopy of three stool samples with or without concentration technique (6.24%), i.e. using the immunoenzymatic method to confirm the presence of G. lamblia antigen in the stool (6.59%). The use of invasive methods in the diagnosis of giardiasis is warranted in individual selected cases, when the significance of the parasite should be assessed regarding its involvement in the pathogenetic mechanisms of damage to the digestive tract mucosa.

An Epidemiological, Diagnostic and Therapeutic Study of Giardia lamblia in Anbar Province – Iraq

2019 ◽  
Vol 9 (01) ◽  
pp. 39-45
Author(s):  
Suad Shallal Shahatha
Keyword(s):  
Teaching Hospital ◽  
Rural Areas ◽  
Giardia Lamblia ◽  
Urban Areas ◽  
Culture Media ◽  
Age Groups ◽  
Stool Samples ◽  
Women And Children ◽  
Teucrium Polium L ◽  
Therapeutic Study

This study was carried out to investigate the epidemiology of Giardia lamblia parasites in patients who visited some of the hospitals in Anbar province, which included (Fallujah Teaching Hospital, Ramadi Teaching Hospital, Ramadi Teaching Hospital for Women and Children and Hit Hospital) during by examining 864 stool samples in a direct examination method, The results revealed the infection rate was 41.7 % and the percentage of infection among males 47.8% is higher than that of females 35.4% with significant differences (p≤0.05). The age groups (1-9) years recorded the highest rates 55.4% and the lowest rate 13.6% in the age group (40-49) years. The highest rate of infection was 62.5% during the month of June, while the month of October was the lowest rate 5% and significant differences. The incidence rate in rural areas was 50.6% higher than in the urban areas 32.5%. The study also included the effect of Teucrium polium L. on the parasite in the culture media HSP-1, the concentrations of 0.5-3 mg / mL significantly affected Giardia, it was noted whenever the greater the concentration, the greater the effect during different treatment periods (1-4) days, as the highest concentration 3 mg/ml killed all Giardia parasites on the fourth day of treatment.

scholarly journals A five year retrospective analysis of common intestinal parasites at Poly Health Center, Gondar, Northwest Ethiopia

2019 ◽  
Author(s):  
Meseret Ayelgn ◽  
Ligabaw Worku ◽  
Getachew Ferede ◽  
Yitayih Wondimeneh
Keyword(s):  
Health Center ◽  
Giardia Lamblia ◽  
Intestinal Parasite ◽  
Intestinal Parasites ◽  
Age Groups ◽  
Northwest Ethiopia ◽  
P Value ◽  
Wet Mount ◽  
Parasite Infections ◽  
Stool Samples

Abstract Objective: Intestinal parasites are present throughout the world in varying degrees of prevalence due to many factors. The aim of this study was to determine the five-year trend prevalence of intestinal prevalence among patients who had been suspected for intestinal parasite infections. A retrospective study was conducted from 2009 to 2013 at Poly Health Center Gondar, Northwest Ethiopia. Samples were examined using direct saline wet mount methods. Statistical analysis was done using SPSS version 20 software and a P-value of < 0.05 was considered statistically significant. The results were presented in tables and graphs. Results: During the study period, a total of 13,329 stool samples were requested for intestinal parasite diagnose and 5510 (41.3%) laboratory-confirmed cases were reported with a fluctuating trend. Ten different parasites were reported in each year with Entamoeba histolytica/dispar (16.8%) being the predominant parasite followed by Giardia lamblia (11.4%) and Ascaris lumbricoides (6.7%). Both males (49%) and females (51%) were equally affected (P=0.14). The intestinal parasite was reported in all age groups in the area but the highest and the lowest prevalence were reported in age groups of 20-29 years and 40-49 years, respectively (26.5% vs 6.4%) (P < 0.001).

scholarly journals A five year retrospective analysis of common intestinal parasites at Poly Health Center, Gondar, Northwest Ethiopia

2019 ◽  
Author(s):  
Meseret Ayelgn ◽  
Ligabaw Worku ◽  
Getachew Ferede ◽  
Yitayih Wondimeneh
Keyword(s):  
Health Center ◽  
Giardia Lamblia ◽  
Intestinal Parasite ◽  
Intestinal Parasites ◽  
Age Groups ◽  
Northwest Ethiopia ◽  
P Value ◽  
Wet Mount ◽  
Parasite Infections ◽  
Stool Samples

Abstract Objective: Intestinal parasites are present throughout the world in varying degrees of prevalence due to many factors. The aim of this study was to determine the five year trend prevalence of intestinal prevalence among patients who had been suspected for intestinal parasite infections. A retrospective study was conducted from 2009 to 2013 at Poly Health Center Gondar, Northwest Ethiopia. Samples were examined using direct saline wet mount methods. Statistical analysis was done using SPSS version 20 software and P-value of < 0.05 was considered statistically significant. The results were presented in tables and graphs. Results : during the study period, a total of 13,329 stool samples were requested for intestinal parasite diagnose and 5510 (41.3%) laboratory confirmed cases were reported with a fluctuating trend. Ten different parasites were reported in each year with Entamoeba histolytica/dispar (16.8%) being the predominant parasite followed by Giardia lamblia (11.4%) and Ascaris lumbricoides ( 6.7%). Both males (49%) and females (51%) were equally affected (P=0.14). Intestinal parasite was reported in all age groups in the area but the highest and the lowest prevalence were reported in age groups of 20-29 years and 40-49 years, respectively (26.5% vs 6.4%) (P < 0.001).

scholarly journals Intestinal Parasitic Infections among Prison Inmates in Kathmandu Nepal

2019 ◽  
Vol 17 (3) ◽  
pp. 382-387
Author(s):  
Prasha Shrestha ◽  
Dhiraj Shrestha ◽  
Dhiraj Thapa Magar ◽  
Ganesh Rai ◽  
Kul Raj Rai ◽  
...  
Keyword(s):  
Giardia Lamblia ◽  
Intestinal Parasites ◽  
Parasitic Infections ◽  
Prison Inmates ◽  
Gender Ratio ◽  
Plastic Container ◽  
Intestinal Parasitic Infections ◽  
Concentration Technique ◽  
International College ◽  
Stool Samples

Background: Prison inmates are at high risk of intestinal parasitic infections. Thus, we studied intestinal parasitic infections among inmates of the Central Jail, Kathmandu, Nepal.Methods: Morning stool samples from 400 inmates (M=282 and F=118), were collected in a clean, dry and wide-mouthed plastic container. The samples were transported to the research laboratory of Shi-Gan International College of Science and Technology, and were fixed using 10% formal saline. Then, samples were processed by formal ether sedimentation concentration technique and were observed microscopically by direct-smear technique.Results: Six percent (24/400) samples were positive for intestinal parasites, with a gender ratio (M:F) 1.7:1. But, co-parasitism was not observed. Intestinal parasitic infections were higher among 21-40 years age-group, 3.5% (14/262). Similarly, intestinal parasitic infections were higher among ‘Dalits’ ethnic group, 21.1% (4/19). As compared to helminths, more protozoans, 62.5% (15/24), were observed. Giardia lamblia, 41.67% (10/24), was the most common protozoans while Trichuris trichiura, 25.0% (6/24), was the most common helminths.Conclusions: Intestinal parasitic infections were lower among the inmates of Central jail, but such conditions cannot be presumed in other peripheral settings. Pure and safe drinking water supply and the effective deworming campaign can further reduce this figure at this setting and peripheral jails across the country.Keywords: Intestinal parasitic infections; IPIs; Nepal; prison inmates.

scholarly journals A 5 year retrospective analysis of common intestinal parasites at Poly Health Center, Gondar, Northwest Ethiopia

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Meseret Ayelgn ◽  
Ligabaw Worku ◽  
Getachew Ferede ◽  
Yitayih Wondimeneh
Keyword(s):  
Health Center ◽  
Giardia Lamblia ◽  
Intestinal Parasite ◽  
Intestinal Parasites ◽  
Age Groups ◽  
Northwest Ethiopia ◽  
P Value ◽  
Wet Mount ◽  
Parasite Infections ◽  
Stool Samples

Abstract Objective Intestinal parasites are present throughout the world in varying degrees of prevalence due to many factors. The aim of this study was to determine the 5-year trend prevalence of intestinal prevalence among patients who had been suspected for intestinal parasite infections. A retrospective study was conducted from 2009 to 2013 at Poly Health Center Gondar, Northwest Ethiopia. Samples were examined using direct saline wet mount methods. Statistical analysis was done using SPSS version 20 software and a P-value of < 0.05 was considered statistically significant. The results were presented in tables and graphs. Results During the study period, a total of 13,329 stool samples were requested for intestinal parasite diagnose and 5510 (41.3%) laboratory-confirmed cases were reported with a fluctuating trend. Ten different parasites were reported in each year with Entamoeba histolytica/dispar (16.8%) being the predominant parasite followed by Giardia lamblia (11.4%) and Ascaris lumbricoides (6.7%). Both males (49%) and females (51%) were equally affected (P = 0.14). The intestinal parasite was reported in all age groups in the area but the highest and the lowest prevalence were reported in age groups of 20–29 years and 40–49 years, respectively (26.5% vs 6.4%) (P < 0.001).

The efficiency of immunoenzyme assay in the diagnosis of lambliosis

Open Medicine ◽  
2010 ◽  
Vol 5 (4) ◽  
pp. 464-469
Author(s):  
Natasa Miladinovic Tasic ◽  
Suzana Tasic ◽  
Ivan Tasic
Keyword(s):  
Stool Sample ◽  
Statistical Significance ◽  
Lower Sensitivity ◽  
Diagnostic Efficiency ◽  
Statistical Parameters ◽  
Concentration Technique ◽  
Stool Samples ◽  
The Difference ◽  
Conventional Microscopy ◽  
Very High

AbstractThe aim of this study is to determine the difference in efficiency of direct immunoenzyme-linked assay (EIA) and conventional microscopy (CM) plus conventional concentration technique (CCT) using comparative analysis in the diagnosis of symptomatic and asymptomatic lambliosis when only one stool sample is to be tested. The study enrolled 577 examinees: 208 patients and 369 asymptomatic examinees. Lambliosis was diagnosed using CM plus CCT (three stool samples) and direct EIA (the first sample). All statistical parameters of the EIA method were 100% in the patients with symptoms of infection. In addition to that, in the group of asymptomatic carriers of Giardia lamblia (G. lamblia) some very high values of these parameters were recorded too, with sensitivity and negative predictive value being both at 100%. In contrast to the EIA method, CM plus CCT of the first stool sample demonstrated significantly lower sensitivity (66.67%) compared to the reference standard. The study did not demonstrate any statistically significant differences in diagnostic efficiancy between the EIA testing of one stool sample and CM plus CCT (p<0.05). However, the observed difference in diagnostic efficiency between the methods was very close to the cut-off value for statistical significance (p=0.06).

scholarly journals A five year retrospective analysis of common intestinal parasites at Poly Health Center, Gondar, Northwest Ethiopia

2019 ◽  
Author(s):  
Meseret Ayelgn ◽  
Ligabaw Worku ◽  
Getachew Ferede ◽  
Yitayih Wondimeneh
Keyword(s):  
Health Center ◽  
Giardia Lamblia ◽  
Intestinal Parasite ◽  
Intestinal Parasites ◽  
Age Groups ◽  
Northwest Ethiopia ◽  
P Value ◽  
Wet Mount ◽  
Parasite Infections ◽  
Stool Samples

Abstract Objective: Intestinal parasites are present throughout the world in varying degrees of prevalence due to many factors. The aim of this study was to determine the five year trend prevalence of intestinal prevalence among patients who had been suspected for intestinal parasite infections. A retrospective study was conducted from 2009 to 2013 at Poly Health Center Gondar, Northwest Ethiopia. Samples were examined using direct saline wet mount methods. Statistical analysis was done using SPSS version 20 software and P-value of < 0.05 was considered statistically significant. The results were presented in tables and graphs. Results: during the study period, a total of 13,329 stool samples were requested for intestinal parasite diagnose and 41.3% laboratory confirmed cases were reported with a fluctuating trend. Ten different parasites were reported in each year with Entamoeba histolytica/dispar (16.8%) being the predominant parasite followed by Giardia lamblia (11.4%) and Ascaris lumbricoides (6.7%). Both males (49%) and females (51%) were equally affected. Intestinal parasite was reported in all age groups in the area but the age group of 10–19 years (44.7%) was more affected. Key words: Intestinal parasite, Trend prevalence, Gondar, Ethiopia

scholarly journals Occurrence of intestinal and urinary parasites among Alkhalawi students, Sudan: A cross-sectional study

2020 ◽  
Vol 8 (4) ◽  
pp. 166-169
Author(s):  
Ali NAG ◽  
Bakhit HA ◽  
Saad MBEA ◽  
Alabeden MMAZ ◽  
Alsadig AA ◽  
...  
Keyword(s):  
Entamoeba Histolytica ◽  
Giardia Lamblia ◽  
Prevalence Rate ◽  
Intestinal Parasites ◽  
Age Groups ◽  
Urine Samples ◽  
Personal Hygiene ◽  
Single Infection ◽  
Preparation Technique ◽  
Concentration Technique

Background: Parasitic diseases were some of the leading causes of global mortality with higher burdens of prevalence in developing countries, especially regions of the world where the contaminated water and poor personal hygiene. The purpose of this study to determine the prevalence rate of intestinal and urinary parasites among Alkhalawi students in Rabak city, White Nile State, Sudan. Materials and methods: In this study, 150 stool and 150 urine samples were collected from Alkhalawi students (all were males). The stool samples were processed by wet preparation, formal ether concentration technique and the urine samples were processed by the sedimentation technique. Results: The results revealed that out of the 150 stool and 150 urine samples, 37 were found positive for intestinal and urinary parasites, this constituted an overall prevalence rate of 12.3%. The highest prevalence rate (28%) was reported among the 11-15 years age groups, while the lowest prevalence rate (0%) was reported among the 21-25 years age groups. The prevalence rate of different parasites was as follow: Giardia lamblia (11.3%), Entamoeba histolytica (8%), Hymenolepis nana (2.7%), Schistosoma haematobium (3.3%). The prevalence of intestinal parasites (21.3%) was higher than urinary parasites (3.3%). The study showed that 24% were infected with single infection and 0.6% were infected with mixed infection. Conclusion: From the result we concluded the intestinal and urinary parasites are highly prevalent among the Alkhalawi students in Rabak city. Giardia lamblia is more likely to occur among the Alkhalawi students followed by Entamoeba histolytica. The highest infection rate was reported among the 11-15 years age groups. Formal ether concentration technique proved to be as efficient more than the wet preparation technique.

The mixed AII and BIII genotypes of human Giardia lamblia isolates circulating in Mazandaran Province, northern Iran

2020 ◽  
Vol 20 ◽  
Author(s):  
Elham Kialashaki ◽  
Mahdi Fakhar ◽  
Mehdi Sharif ◽  
Ahmad Daryani ◽  
Reza Saberi
Keyword(s):  
Abdominal Pain ◽  
Giardia Lamblia ◽  
Clinical Symptoms ◽  
Statistical Tests ◽  
Health Centers ◽  
Mazandaran Province ◽  
Northern Iran ◽  
High Prevalence ◽  
Pcr Rflp ◽  
Stool Samples

Objectives: Giardiasis is one of the human parasitic diseases by a flagellate protozoan named Giardia lamblia. This parasite is one of the most common organisms causing diarrhea in human and a common gastrointestinal parasite in vertebrates. The purpose of this investigation was to determine the molecular epidemiology of Giardia lamblia (G.lamblia) by PCR-RFLP method in the Mazandaran Province and to investigate possible relationship between parasite genotype and clinical symptoms. Methods: In this study, 50 stool samples were collected from human cases of G. lamblia. referred to health centers in Sari, Babol, Nowshahr cities. The samples were examined by formalin-ether concentration procedure. DNA extraction was carried out on samples containing adequate Giardia cysts, and then PCR-RFLP was done on glutamate dehydrogenase (gdh) marker. Results: In these patients, the most common signs were abdominal pain (42.9%), diarrhea (47.6%) and least marked common signs was malaise (4.7%). Of all 21 isolates, 9 samples (42.8%) were found as genotype mixed AII and BIII, 6 samples (28.6%) belonged to genotype BIII and 6 ones (28.6%) were genotype AII. Additionally, there was a significant correlation between AII genotype and clinical symptoms (P <0.05). According to the statistical tests there was a significant relation between abdominal pain and intensity of infection (P<0.05). Conclusion: However, genotype AII and mixed AII and BIII genotypes were more common respectively. The relatively high prevalence of mixed infection of these genotypes indicated the potential of zoonotic source of giardiasis among patients in Mazandaran Province.

scholarly journals High prevalence of selected viruses and parasites and their predictors in Malawian children

2019 ◽  
Vol 147 ◽  
Author(s):  
Y.-M. Fan ◽  
S. Oikarinen ◽  
K.-M. Lehto ◽  
N. Nurminen ◽  
R. Juuti ◽  
...  
Keyword(s):  
Infection Rate ◽  
Giardia Lamblia ◽  
Parasitic Infections ◽  
Environmental Predictors ◽  
Chain Reaction ◽  
Child Undernutrition ◽  
Bacterial Microbiota ◽  
High Prevalence ◽  
Stool Samples ◽  
Polymerase Chain

AbstractEnteric pathogens have been related to child undernutrition. Whereas there are lots of data on enteric bacterial microbiota and infections, much less is known about the incidence of prevalence of intestinal colonisation with viruses or important parasitic species. This study assessed the presence of selected viruses and parasites in stools of 469, 354, 468 Malawian children at 6, 12 and 18 months. We also assessed environmental predictors of the presence of viruses and parasites among 6-month infants. Microbial presence was documented using real-time polymerase chain reaction (PCR). Enteroviruses were identified in 68%, 80% and 81% of the stool samples at 6, 12 and 18 months children, rhinovirus in 28%, 18% and 31%, norovirus in 24%, 22% and 16%, parechovirus in 23%, 17% and 17%, rotavirus in 3%, 1% and 0.6%,Giardia lambliain 9.6%, 23.5% and 26%, andCryptosporidium(spp.) in 6%, 8% and 2% of the 6, 12 and 18 months stool samples. Dry season (May–October) was associated with a low infection rate of enterovirus, norovirus andCryptosporidium(spp.). Higher father's education level, less number of person in the household and higher sanitation were associated with a low infection rate of enterovirus, norovirus and rotavirus, respectively. The results suggest that the prevalence of asymptomatic viral and parasitic infections is high among Malawian children and that the family's living conditions and seasonality influence the rate of infections.

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