scholarly journals Molecular Characterization of Multi Drug Resistant (MDR) Extended Spectrum βlactamase (ESBL) Producing Klebsiella spp. from Pond Water in West Bengal

2020 ◽  
Vol 64 (02) ◽  
pp. 80-86
Author(s):  
D. Bhattacharyya ◽  
S. Saha ◽  
J. Banerjee ◽  
S. Bandyopadhyay ◽  
K. Sarkar
2016 ◽  
Vol 49 (5) ◽  
pp. 692-700 ◽  
Author(s):  
Cheng-Yen Kao ◽  
Uuganbayar Udval ◽  
Yi-Ting Huang ◽  
Hsiu-Mei Wu ◽  
Ay-Huey Huang ◽  
...  

Author(s):  
Samira Natoubi ◽  
Abouddihaj Barguigua ◽  
Nezha Baghdad ◽  
Kaotar Nayme ◽  
Mohammed Timinouni ◽  
...  

ABSTRACTObjective: Urinary tract infections (UTIs) are still commonly diagnosed in outpatients as well as in hospitalized patients. In this study, weinvestigated the prevalence and performed molecular characterization of extended-spectrum-β-lactamases (ESBL) and carbapenemases produced byEnterobacteriaceae isolates that cause community UTIs in Settat city, Morocco.Methods: From January 2012 to December 2013, all uropathogenic community Enterobacteriaceae isolates were collected from the microbiologylaboratory of Hassan II Hospital, Settat, Morocco. Antibiotic susceptibility testing was performed as recommended by Clinical and LaboratoryStandard Institute. Phenotypic identification of ESBL and carbapenemase producer isolates was confirmed by the double-disk synergy test and themodified Hodge test, respectively. Molecular characterization of β-lactamase genes was performed using polymerase chain reaction (PCR), followedby sequencing of the obtained products.Results: Among 153 isolates, 31 (20.26%) were multi-drug resistant (MDR). Nine strains (5.88%) were ESBL producers, of which Klebsiellapneumoniae (n=5; 20.83%), Escherichia coli (n=3; 3.15%), and Enterobacter cloacae (n=1; 9.09%) species were identified. The results of ESBLencodinggenedetectionby sequencing revealed the presence of CTX-M-15 (n=9) in association with other β-lactamase genes such as temoneira 1(n=8) and sulfhydryl variable 1 (n=5). According to the modified Hodge test and PCR, three isolates (1.96%) were positive carry the blaConclusion: The emergence of MDR uropathogenic Enterobacteriaceae isolates in our community is highly alarming. Strict measures will be requiredto control the further spread of these uropathogenic isolates.Keywords: Carbapenemase, Enterobacteriaceae, Extended-spectrum-β-lactamases, Moroccan community.OXA-48 gene.


2013 ◽  
Vol 20 (5) ◽  
pp. 1015-1022
Author(s):  
Shaowu LI ◽  
Di WANG ◽  
Hongbai LIU ◽  
Jiasheng YIN ◽  
Tongyan LU

One Health ◽  
2021 ◽  
pp. 100236
Author(s):  
Marília Salgado-Caxito ◽  
Julio A. Benavides ◽  
Aiko D. Adell ◽  
Antonio Carlos Paes ◽  
Andrea I. Moreno-Switt

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