Antioxidant Activities of Hot Water Extract from Cornus walteri Wanger against Oxidative Stress Induced by tert-Butyl Hydroperoxide in HepG2 Cells

2013 ◽  
Vol 42 (10) ◽  
pp. 1525-1532 ◽  
Author(s):  
Seong Ho Yeon ◽  
Hyeonmi Ham ◽  
Jeehye Sung ◽  
Younghwa Kim ◽  
Seulgi Namkoong ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hepg2 Cells ◽  
Antioxidant Activities ◽  
Water Extract ◽  
Hot Water ◽  
Butyl Hydroperoxide ◽  
Tert Butyl ◽  
Tert Butyl Hydroperoxide ◽  
Hot Water Extract

scholarly journals Meroterpenoid-Rich Fraction of the Ethanolic Extract from Sargassum serratifolium Suppressed Oxidative Stress Induced by Tert-Butyl Hydroperoxide in HepG2 Cells

Marine Drugs ◽  
2018 ◽  
Vol 16 (10) ◽  
pp. 374 ◽  
Author(s):  
Sujin Lim ◽  
Misung Kwon ◽  
Eun-Ji Joung ◽  
Taisun Shin ◽  
Chul-Woong Oh ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hepg2 Cells ◽  
Ethanolic Extract ◽  
Glutathione Depletion ◽  
Heme Oxygenase 1 ◽  
Dependent Manner ◽  
Butyl Hydroperoxide ◽  
Tert Butyl ◽  
Tert Butyl Hydroperoxide ◽  
Sargassum Serratifolium

Sargassum species have been reported to be a source of phytochemicals, with a wide range of biological activities. In this study, we evaluated the hepatoprotective effect of a meroterpenoid-rich fraction of the ethanolic extract from Sargassum serratifolium (MES) against tert-butyl hydroperoxide (t-BHP)-treated HepG2 cells. Treatment with MES recovered the cell viability from the t-BHP-induced oxidative damage in a dose-dependent manner. It suppressed the reactive oxygen species production, lipid peroxidation, and glutathione depletion in the t-BHP-treated HepG2 cells. The activity of the antioxidants induced by t-BHP, including superoxide dismutase (SOD) and catalase, was reduced by the MES treatment. Moreover, it increased the nuclear translocation of nuclear factor erythroid 2-related factor 2, leading to the enhanced activity of glutathione S transferase, and the increased production of heme oxygenase-1 and NAD(P)H:quinine oxidoreductase 1 in t-BHP-treated HepG2 cells. These results demonstrate that the antioxidant activity of MES substituted the activity of the SOD and catalase, and induced the production of detoxifying enzymes, indicating that MES might be used as a hepatoprotectant against t-BHP-induced oxidative stress.

scholarly journals Selenium methylselenocysteine protects human hepatoma HepG2 cells against oxidative stress induced by tert-butyl hydroperoxide

2007 ◽  
Vol 389 (7-8) ◽  
pp. 2167-2178 ◽  
Author(s):  
Susana Cuello ◽  
Sonia Ramos ◽  
Raquel Mateos ◽  
M. Angeles Martín ◽  
Yolanda Madrid ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hepg2 Cells ◽  
Human Hepatoma ◽  
Butyl Hydroperoxide ◽  
Tert Butyl ◽  
Tert Butyl Hydroperoxide ◽  
Human Hepatoma Hepg2 Cells ◽  
Hepatoma Hepg2

scholarly journals Kushenol C Prevents Tert-Butyl Hydroperoxide and Acetaminophen-Induced Liver Injury

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1635
Author(s):  
Byoung Ok Cho ◽  
Jang Hoon Kim ◽  
Denis Nchang Che ◽  
Hyun Ju Kang ◽  
Jae Young Shin ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Liver Injury ◽  
Hepg2 Cells ◽  
Caspase 3 ◽  
Liver Lipid ◽  
Ros Generation ◽  
Butyl Hydroperoxide ◽  
Tert Butyl ◽  
Proinflammatory Mediators ◽  
Tert Butyl Hydroperoxide

Sophora flavescens, also known as Kushen, has traditionally been used as a herbal medicine. In the present study we evaluated the ameliorative effects of kushenol C (KC) from S. flavescens against tBHP (tert-Butyl hydroperoxide)-induced oxidative stress in hepatocellular carcinoma (HEPG2) cells and acetaminophen (APAP)-induced hepatotoxicity in mice. KC pretreatment protected the HEPG2 cells against oxidative stress by reducing cell death, apoptosis and reactive oxygen species (ROS) generation. KC pretreatment also upregulated pro-caspase 3 and GSH (glutathione) as well as expression of 8-Oxoguanine DNA Glycosylase (OGG1) in the HEPG2 cells. The mechanism of action was partly related by KC’s activation of Akt (Protein kinase B (PKB)) and Nrf2 (Nuclear factor (erythroid-derived 2)-like 2) in the HepG2 cells. In in vivo investigations, coadministration of mice with KC and APAP significantly attenuated APAP-induced hepatotoxicity and liver damage, as the serum enzymatic activity of aspartate aminotransferase and alanine aminotransferase, as well as liver lipid peroxidation and cleaved caspase 3 expression, were reduced in APAP-treated mice. Coadministration with KC also up-regulated antioxidant enzyme expression and prevented the production of proinflammatory mediators in APAP-treated mice. Taken together, these results showed that KC treatment has potential as a therapeutic agent against liver injury through the suppression of oxidative stress.

scholarly journals Protective Effects of Black Rice Extracts on Oxidative Stress Induced by tert-Butyl Hydroperoxide in HepG2 Cells

2014 ◽  
Vol 19 (4) ◽  
pp. 348-352 ◽  
Author(s):  
Seon-Mi Lee ◽  
Youngmin Choi ◽  
Jeehye Sung ◽  
Younghwa Kim ◽  
Heon-Sang Jeong ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hepg2 Cells ◽  
Protective Effects ◽  
Black Rice ◽  
Butyl Hydroperoxide ◽  
Tert Butyl ◽  
Tert Butyl Hydroperoxide

A Tocotrienol-Rich Fraction from Grape Seeds Inhibits Oxidative Stress Induced by tert-Butyl Hydroperoxide in HepG2 Cells

2010 ◽  
Vol 13 (5) ◽  
pp. 1240-1246 ◽  
Author(s):  
Youngmin Choi ◽  
Seon-Mi Lee ◽  
Younghwa Kim ◽  
Jaemin Yoon ◽  
Heon-Sang Jeong ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hepg2 Cells ◽  
Grape Seeds ◽  
Butyl Hydroperoxide ◽  
Tert Butyl ◽  
Tert Butyl Hydroperoxide ◽  
Tocotrienol Rich Fraction

scholarly journals A hot water extract of turmeric (Curcuma longa) suppresses acute ethanol-induced liver injury in mice by inhibiting hepatic oxidative stress and inflammatory cytokine production

2017 ◽  
Vol 6 ◽  
Author(s):  
Ryusei Uchio ◽  
Yohei Higashi ◽  
Yusuke Kohama ◽  
Kengo Kawasaki ◽  
Takashi Hirao ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Liver Injury ◽  
Oral Administration ◽  
Alanine Aminotransferase ◽  
Curcuma Longa ◽  
Water Extract ◽  
Hot Water ◽  
Hepatic Tissue ◽  
Hot Water Extract ◽  
Acute Ethanol

AbstractTurmeric (Curcuma longa) is a widely used spice that has various biological effects, and aqueous extracts of turmeric exhibit potent antioxidant activity and anti-inflammatory activity. Bisacurone, a component of turmeric extract, is known to have similar effects. Oxidative stress and inflammatory cytokines play an important role in ethanol-induced liver injury. This study was performed to evaluate the influence of a hot water extract of C. longa (WEC) or bisacurone on acute ethanol-induced liver injury. C57BL/6 mice were orally administered WEC (20 mg/kg body weight; BW) or bisacurone (60 µg/kg BW) at 30 min before a single dose of ethanol was given by oral administration (3·0 g/kg BW). Plasma levels of aspartate aminotransferase and alanine aminotransferase were markedly increased in ethanol-treated mice, while the increase of these enzymes was significantly suppressed by prior administration of WEC. The increase of alanine aminotransferase was also significantly suppressed by pretreatment with bisacurone. Compared with control mice, animals given WEC had higher hepatic tissue levels of superoxide dismutase and glutathione, as well as lower hepatic tissue levels of thiobarbituric acid-reactive substances, TNF-α protein and IL-6 mRNA. These results suggest that oral administration of WEC may have a protective effect against ethanol-induced liver injury by suppressing hepatic oxidation and inflammation, at least partly through the effects of bisacurone.

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