Uric acid enhances T cell immune responses to hepatitis B surface antigen-pulsed-dendritic cells in mice

We have previously demonstrated that the murine humoral immune responses to the group-specific a and subtype-specific d/y determinants of hepatitis B surface antigen (HBsAg) are controlled by H-2-linked immune response (Ir) genes. High responder (H-2d,q), intermediate responder (H-2a greater than b greater than k) and nonresponder (H-2f,s) haplotypes have been identified (8, 9). The kinetics and specificity of in vivo antibody production after HBsAg immunization in congeneic, H-2-recombinant strains was analyzed to further define relevant Ir genes and their influence on the immune response to distinct antigenic determinants. These studies indicate that the humoral anti-HBs response is regulated by at least two Ir genes, one in the I-A subregion (Ir-HBs-1) and one in the I-C subregion (Ir-HBs-2) of the murine H-2 complex. Ir-HBs-1 regulates the primary responses to all HBsAg determinants, whereas the influence of Ir-HBs-2 is determinant specific, affecting the responses to the d or y determinants. The anti-a response is regulated exclusively by Ir-HBs-1. Strains possessing only the Ir-HBs-2 gene [B10.S(9R) and B10.HTT] produce no anti-a response and a subtype-specific antibody response is detected only after secondary or tertiary immunization. In contrast, the influence of Ir-HBs-2 in the presence of Ir-HBs-1 is detected upon primary immunization and is additive rather than exclusive. There is also suggestive evidence that the presence of the Ek molecule, at least in the context of I-Ak, may have a suppressive influence on the anti-HBs response. Additionally, HBsAg-specific, T cell proliferative responses were H-2 restricted and the kinetics and specificity of T cell proliferative responses paralleled in vivo antibody production. These data indicate that, although the I-A subregion exerts a dominant influence, distinct Ir-HBs genes, mapping in separate I subregions, control immune responses to alternate HBsAg determinants on the same protein molecule.

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MONOCYTES, MACROPHAGES, DENDRITIC AND MYELOID SUPPRESSOR CELLS: GENESIS, CLASSIFICATION, IMMUNOBIOLOGICAL PROPERTIES

Proceedings of the National Academy of Sciences of Belarus Medical series ◽

10.29235/1814-6023-2018-15-3-363-382 ◽

2018 ◽

Vol 15 (3) ◽

pp. 363-382

Author(s):

L. P. Titov

Keyword(s):

Dendritic Cells ◽

T Cell ◽

Immune Responses ◽

Autoimmune Diseases ◽

Suppressor Cells ◽

Mononuclear Phagocyte ◽

Natural Immunity ◽

Myeloid Suppressor Cells ◽

T Cell Immune Responses

The article presents the modern data on the most important component of natural immunity – cells of the mononuclear phagocyte system. The questions of origin, the spectrum of expressed markers of differentiation, the classification of monocytes (classical, intermediate, non-classical), macrophages (pro-inflammatory and anti-inflammatory) and dendritic cells (myeloid, plasmacytoid), their immunobiological functions, their role in humoral and T-cell immune responses, anergy and tolerance are considered. The possibility of obtaining cellular immunobiological products (adjuvant and tolerogenic) for immunotherapy of oncological, infectious and autoimmune diseases on their basis is analyzed.

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