scholarly journals Effects of α-mangostin on apoptosis induction of human colon cancer

2011 ◽  
Vol 17 (16) ◽  
pp. 2086 ◽  
Author(s):  
Ramida Watanapokasin
Keyword(s):  
Colon Cancer ◽  
Human Colon ◽  
Apoptosis Induction ◽  
Human Colon Cancer

EFFECT OF VALPROIC ACID IN COMPARISON WITH VORINOSTAT ON CELL GROWTH INHIBITION AND APOPTOSIS INDUCTION IN THE HUMAN COLON CANCER SW48 CELLS IN VITRO

2018 ◽  
Vol 40 (2) ◽  
pp. 95-100 ◽  
Author(s):  
M Sanaei ◽  
F Kavoosi ◽  
O Mansoori
Keyword(s):  
Colon Cancer ◽  
Valproic Acid ◽  
Cell Growth ◽  
Growth Inhibition ◽  
Histone Deacetylases ◽  
Regulation Of Gene Expression ◽  
Human Colon ◽  
Apoptosis Induction ◽  
Cell Growth Inhibition ◽  
Human Colon Cancer

Aim: Acetylation levels of histones are the result of the balance between histone acetyltransfrases and histone deacetylases activities, which plays an important role in chromatin remodeling and regulation of gene expression. Histone deacetylases inhibitors such as valproic acid, vorinostat have attracted interest because of their ability to induce differentiation and apoptosis of cancer cells. The current study was designed to assess the effect of valproic acid in comparison to and in combination with vorinostat on cell growth inhibition and apoptosis induction in the human colon cancer SW48 cells. Materials and Methods: The colon cancer SW48 cells were seeded and treated with various doses of valproic acid and vorinostat and MTT assay and flow cytometric assay were done to determine cell viability and cell apoptosis, respectively. Results: All concentrations of both agents reduced viability significantly in a dose- and time-dependent fashion (p < 0.004). Both compounds, either single or combined agents, induced apoptosis significantly, whereas the ratio of the apoptotic cells treated with combined agents was more significant than the single. Conclusion: Our findings suggest that vaproic acid and vorinostat can significantly inhibit cell growth and induce apoptosis in colon cancer SW48 cells.

scholarly journals Apoptotic Cell Death and Inhibition of Wnt/β-Catenin Signaling Pathway in Human Colon Cancer Cells by an Active Fraction (HS7) fromTaiwanofungus camphoratus

2011 ◽  
Vol 2011 ◽  
pp. 1-13 ◽  
Author(s):  
Chi-Tai Yeh ◽  
Chih-Jung Yao ◽  
Jiann-Long Yan ◽  
Shuang-En Chuang ◽  
Liang-Ming Lee ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Human Colon ◽  
Dependent Manner ◽  
Apoptosis Induction ◽  
Colon Cancer Cells ◽  
Active Fraction ◽  
Human Colon Cancer ◽  
Taiwanofungus Camphoratus ◽  
Human Colon Cancer Cells

Aberrant activation of Wnt/β-catenin signaling plays an important role in the development of colon cancer. HS7 is an active fraction extracted fromTaiwanofungus camphoratus, which had been widely used as complementary medicine for Taiwan cancer patients in the past decades. In this study, we demonstrated the effects of HS7 on the growth inhibition, apoptosis induction, and Wnt/β-catenin signaling suppression in human colon cancer cells. HS7 significantly inhibited proliferation of HT29, HCT116, and SW480 colon cancer cells in a dose- and time-dependent manner. The apoptosis induction was evidenced by DNA fragmentation and subG1 accumulation, which was associated with increased Bax/Bcl-2 ratio, activation of caspase-3 and cleavage of PARP. By using Tcf-dependent luciferase activity assay, HS7 was found to inhibit theβ-catenin/Tcf transcriptional activities. In addition, HS7 strongly suppressed the binding of Tcf complexes to its DNA-binding site shown in electrophoretic mobility shift assay. This inhibition was further confirmed by the decreased protein levels of Tcf-4 andβ-catenin. Theβ-catenin/Tcf downstream target genes, such assurvivin,c-myc,cyclin D1,MMP7, andMT1-MMPinvolved in apoptosis, invasion, and angiogenesis were also diminished as well. These results indicate thatTaiwanofungus camphoratusmay provide a benefit as integrative medicine for the treatment of colon cancer.

scholarly journals Impact of Procyanidins from Different Berries on Caspase 8 Activation in Colon Cancer

2015 ◽  
Vol 2015 ◽  
pp. 1-13 ◽  
Author(s):  
Carole Minker ◽  
Livine Duban ◽  
Daniel Karas ◽  
Päivi Järvinen ◽  
Annelise Lobstein ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Human Colon ◽  
Cancer Cell Lines ◽  
Human Colon Cancer Cell ◽  
Apoptosis Induction ◽  
Human Colon Cancer ◽  
Lowbush Blueberry ◽  
Colon Cancer Cell Lines

Scope. The aim of this work is to identify which proapoptotic pathway is induced in human colon cancer cell lines, in contact with proanthocyanidins extracted from various berries.Methods and Results. Proanthocyanidins (Pcys) extracted from 11 berry species are monitored for proapoptotic activities on two related human colon cancer cell lines: SW480-TRAIL-sensitive and SW620-TRAIL-resistant. Apoptosis induction is monitored by cell surface phosphatidylserine (PS) detection. Lowbush blueberry extract triggers the strongest activity. When tested on the human monocytic cell line THP-1, blueberry Pcys are less effective for PS externalisation and DNA fragmentation is absent, highlighting a specificity of apoptosis induction in gut cells. In Pcys-treated gut cell lines, caspase 8 (apoptosis extrinsic pathway) but not caspase 9 (apoptosis intrinsic pathway) is activated after 3 hours through P38 phosphorylation (90 min), emphasizing the potency of lowbush blueberry Pcys to eradicate gut TRAIL-resistant cancer cells.Conclusion. We highlight here that berries Pcys, especially lowbush blueberry Pcys, are of putative interest for nutritional chemoprevention of colorectal cancer in view of their apoptosis induction in a human colorectal cancer cell lines.

Anticancer potential of an exopolysaccharide from Lactobacillus helveticus MB2-1 on human colon cancer HT-29 cells via apoptosis induction

2020 ◽  
Vol 11 (11) ◽  
pp. 10170-10181
Author(s):  
Luyao Xiao ◽  
Xiaojia Ge ◽  
Lin Yang ◽  
Xiaohong Chen ◽  
Qian Xu ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Human Colon ◽  
Lactobacillus Helveticus ◽  
Apoptosis Induction ◽  
Human Colon Cancer ◽  
Ht 29

LHEPS-1 exhibited the strongest anti-proliferation effect, prevented G1 to S transition and increased the apoptosis of human colon cancer HT-29 cells.

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