Enhanced inhibition of influenza virus infection by peptide–noble-metal nanoparticle conjugates

The influenza (“flu”) type-A virus is a major medical and veterinary health concern and causes global pandemics. The peptide “FluPep” is an established inhibitor of influenza virus infectivity in model systems. We have explored the potential for noble-metal nanoparticle conjugates of FluPep to enhance its antiviral activity and to determine their potential as a delivery platform for FluPep. FluPep ligand is FluPep extended at its N-terminus with the sequence CVVVTAAA, to allow for its incorporation into a mixed-matrix ligand shell of a peptidol and an alkanethiol ethylene glycol consisting of 70% CVVVTol and 30% HS(CH2)11(OC2H4)4OH (mol/mol). Gold and silver nanoparticles (ca. 10 nm diameter) with up to 5% (mol/mol) FluPep ligand remained as stable as the control of mixed-matrix-passivated nanoparticles in a variety of tests, including ligand exchange with dithiothreitol. The free FluPep ligand peptide was found to inhibit viral plaque formation in canine MDCK cells (IC50 = 2.1 nM), but was less potent than FluPep itself (IC50 = 140 pM). Nanoparticles functionalised with FluPep ligand showed enhanced antiviral activity compared to the free peptides. The IC50 value of the FluPep-functionalised nanoparticles decreased as the grafting density of FluPep ligand increased from 0.03% to 5% (both mol/mol), with IC50 values down to about 10% of that of the corresponding free peptide. The data demonstrate that conjugation of FluPep to gold and silver nanoparticles enhances its antiviral potency; the antimicrobial activity of silver ions may enable the design of even more potent antimicrobial inhibitors, capable of targeting both influenza and bacterial co-infections.

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Enhanced inhibition of influenza virus infection by peptide-noble metal nanoparticle conjugates

10.1101/324939 ◽

2018 ◽

Author(s):

Zaid K. Alghrair ◽

David G. Fernig ◽

Bahram Ebrahimi

Keyword(s):

Silver Nanoparticles ◽

Influenza Virus ◽

Antiviral Activity ◽

Noble Metal ◽

Metal Nanoparticle ◽

Model Systems ◽

Ligand Concentration ◽

Health Concern ◽

Virus Infectivity ◽

Gold And Silver Nanoparticles

AbstractInfluenza virus is a major medical and veterinary health concern and causes global pandemics. The peptide ‘FluPep’ is an established inhibitor of influenza virus infectivity in model systems. We have explored the potential for FluPep functionalised noble metal nanoparticle to enhance the antiviral activity of the peptide Flupep and determined their potential for the delivery of FluPep. The FluPep ligand designed here is FluPep extended at its N-terminus with the sequence CVVVTAAA-, to allow its incorporation into a mix matrix ligand shell of a peptidol and an alkanethiol ethyleneglycol comprising 70% H-CVVVTol and 30% HS(CH2)11(ethyleneglycol)4 (both mole/mole). Gold and silver nanoparticles (~10 nm diameter) prepared with up to 5% (mole/mole) FluPep ligand contained in the mixture of mix-matrix peptide ligands remained as stable as the control mix-matrix coated nanoparticles against ligand exchange with dithiothreitol. FluPep ligand was found to inhibit viral plaque formation in canine MDCK cells (IC50 2.1 nM), but was less potent than FluPep itself (IC50 140 pM). FluPep ligand functonalised nanoparticles retained antiviral activity in the plaque assay. Moreover, at low grafting densities (where nanoparticles incorporate ~1 FluPep ligand, the antiviral potency in terms of FluPep ligand concentration was enhanced significantly for gold and silver nanoparticles (IC50 ~8-fold and ~3-fold lower, respectively). At higher grafting density the potency relative to free FluPep ligand concentration decreased. The data demonstrate that conjugation of FluPep to gold and silver nanoparticles enhances its antiviral potency; the antimicrobial activity of silver ions may enable the design of even more potent anti-microbial inhibitors.

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Current Strategies for Noble Metal Nanoparticle Synthesis

Nanoscale Research Letters ◽

10.1186/s11671-021-03480-8 ◽

2021 ◽

Vol 16 (1) ◽

Author(s):

Giyaullah Habibullah ◽

Jitka Viktorova ◽

Tomas Ruml

Keyword(s):

Noble Metal ◽

Noble Metals ◽

Drug Carriers ◽

Industrial Applications ◽

Metal Nanoparticle ◽

Noble Metal Nanoparticles ◽

Synthesis Methods ◽

Gold And Silver Nanoparticles ◽

Diverse Range ◽

The Past

AbstractNoble metals have played an integral part in human history for centuries; however, their integration with recent advances in nanotechnology and material sciences have provided new research opportunities in both academia and industry, which has resulted in a new array of advanced applications, including medical ones. Noble metal nanoparticles (NMNPs) have been of great importance in the field of biomedicine over the past few decades due to their importance in personalized healthcare and diagnostics. In particular, platinum, gold and silver nanoparticles have achieved the most dominant spot in the list, thanks to a very diverse range of industrial applications, including biomedical ones such as antimicrobial and antiviral agents, diagnostics, drug carriers and imaging probes. In particular, their superior resistance to extreme conditions of corrosion and oxidation is highly appreciated. Notably, in the past two decades there has been a tremendous advancement in the development of new strategies of more cost-effective and robust NMNP synthesis methods that provide materials with highly tunable physicochemical, optical and thermal properties, and biochemical functionalities. As a result, new advanced hybrid NMNPs with polymer, graphene, carbon nanotubes, quantum dots and core–shell systems have been developed with even more enhanced physicochemical characteristics that has led to exceptional diagnostic and therapeutic applications. In this review, we aim to summarize current advances in the synthesis of NMNPs (Au, Ag and Pt).

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Antiviral activity of plant juices and green tea against SARS-CoV-2 and influenza virus in vitro

10.1101/2020.10.30.360545 ◽

2020 ◽

Cited By ~ 1

Author(s):

Bruno Frank ◽

Carina Conzelmann ◽

Tatjana Weil ◽

Rüdiger Groß ◽

Peggy Jungke ◽

...

Keyword(s):

Influenza Virus ◽

Antiviral Activity ◽

Green Tea ◽

Human Adenovirus ◽

Virus Infectivity ◽

Virucidal Activity ◽

Black Chokeberry ◽

Chokeberry Juice

AbstractMany plant juices, extracts and teas have been shown to possess antiviral activity. We here analyzed the virucidal activity of black chokeberry (Aronia melanocarpa), pomegranate (Punica granatum), and elderberry (Sambucus nigra) juice, as well as green tea (Camellia sinensis) against different respiratory viruses. We found that all tested plant derived products effectively inactivated influenza virus, whereas only chokeberry juice diminished SARS-CoV-2 and vaccinia virus infectivity. None of the products inactivated non-enveloped human adenovirus type 5. Thus, black chokeberry juice exerts virucidal activity against different enveloped viral pathogens under in vitro conditions. Whether application of virucidal juices or green tea as oral rinses may lower viral loads in the oral cavity in vivo remains to be evaluated.

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Metal nanoparticle assisted polymerase chain reaction for strain typing of Salmonella Typhi

The Analyst ◽

10.1039/c5an01286d ◽

2015 ◽

Vol 140 (21) ◽

pp. 7366-7372 ◽

Cited By ~ 9

Author(s):

Asma Rehman ◽

Yasra Sarwar ◽

Zulfiqar Ali Raza ◽

Syed Zajif Hussain ◽

Tanveer Mustafa ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Silver Nanoparticles ◽

Salmonella Typhi ◽

Metal Nanoparticle ◽

Strain Typing ◽

Chain Reaction ◽

Gold And Silver Nanoparticles ◽

Specific Amplification ◽

Polymerase Chain

Gold and silver nanoparticles are demonstrated to reduce non-specific amplification during PCR based strain typing ofSalmonellaTyphi.

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CHEMICAL INTERACTIONS AT NOBLE METAL NANOPARTICLE SURFACES — CATALYSIS, SENSORS AND DEVICES

COSMOS ◽

10.1142/s0219607707000244 ◽

2007 ◽

Vol 03 (01) ◽

pp. 103-124 ◽

Cited By ~ 16

Author(s):

A. SREEKUMARAN NAIR ◽

RENJIS T. TOM ◽

V. R. RAJEEV KUMAR ◽

C. SUBRAMANIAM ◽

T. PRADEEP

Keyword(s):

Noble Metal ◽

Metal Nanoparticle ◽

Noble Metal Nanoparticles ◽

Chemical Interactions ◽

Surface Binding ◽

Flow Sensors ◽

Gold And Silver Nanoparticles ◽

Cyt C ◽

Tunable Optical Properties ◽

Sensing Characteristics

In this paper, a summary of some of the recent research efforts in our laboratory on chemical interactions at noble metal nanoparticle surfaces is presented. The article is divided into five sections, detailing with (i) interactions of simple halocarbons with gold and silver nanoparticle surfaces at room temperature by a new chemistry and the exploitation of this chemistry in the extraction of pesticides from drinking water, (ii) interaction of biologically important proteins such as Cyt c, hemoglobin and myoglobin as well as a model system, hemin with gold and silver nanoparticles and nanorods forming nano–bio conjugates and their surface binding chemistry, (iii) formation of polymer–nano composites with tunable optical properties and temperature sensing characteristics by single and multi-step methodologies, (iv) nanomaterials-based flow sensors and (v) composites of noble metal nanoparticles and metallic carbon nanotubes showing visible fluorescence induced by metal–semiconductor transition.

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Complex coloration and antibacterial functionalization of silk fabrics based on noble metal nanoparticles

Journal of Engineered Fibers and Fabrics ◽

10.1177/1558925019866948 ◽

2019 ◽

Vol 14 ◽

pp. 155892501986694 ◽

Cited By ~ 1

Author(s):

Zhanyu Zhang ◽

Xiaodan Lv ◽

Qidan Chen ◽

Jing An

Keyword(s):

Silver Nanoparticles ◽

Metal Nanoparticles ◽

Noble Metal ◽

Fiber Surface ◽

Noble Metal Nanoparticles ◽

Localized Surface Plasmon ◽

Color Strength ◽

Gold And Silver Nanoparticles ◽

Silk Fabrics

Noble metal nanoparticles and traditional dyes jointly colorate silk fabrics. Gold and silver nanoparticles were in situ synthesized on silk fabrics, and the complex coloration was realized by the integration of traditional dyes into the treated silk. The localized surface plasmon resonance properties of gold and silver nanoparticles were not affected by the coloration of dyed-on silk fabrics. The combined optical properties of nanoparticles and dyes extended the color range and enhanced the color strength (K/S) of silk fabrics. Ultraviolet–visible diffuse absorption spectroscopy and scanning electron microscopy demonstrated the in situ synthesis of gold and silver nanoparticles on silk fabrics. The coloration of traditional dyes influenced the morphologies of gold and silver nanoparticles on fiber surface slightly. The silk fabrics with complex coloration showed significant antibacterial property. The complex coloration based on particular nanoparticles and dyes provides a promising strategy to develop multifunctional textiles.

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Attenuation of Interferon Responses in the Obese Host and Ramifications for Influenza Virus Evolution

10.21007/etd.cghs.2020.0519 ◽

2020 ◽

Author(s):

◽

Rebekah Honce ◽

Keyword(s):

Influenza Virus ◽

Influenza Virus Infection ◽

Communicable Diseases ◽

Influenza Viruses ◽

Model Systems ◽

Health Concern ◽

Viral Population ◽

High Morbidity ◽

Type I ◽

The Impact

The most insidious pandemic of modern life does not arise from an infectious agent but rather from malnutrition. With its global incidence tripling over the past three decades, obesity is a major public health concern. Obesity’s rising prevalence has also illuminated its impact on communicable diseases. Following the 2009 H1N1 influenza A virus pandemic, obesity was identified as a risk factor for increased disease severity and mortality in infected individuals. Obesity causes a chronic state of meta-inflammation with systemic implications for immunity, including delayed antiviral responses to influenza virus infection, poor recovery, and impaired immunological memory. However, the majority of past work neglected the innate responses, especially the impact on the epithelium. To better understand the innate response to influenza infection, obese animal models and human respiratory epithelial cells were inoculated with influenza viruses to quantify the innate responses. In obese hosts, antiviral effector production, including type I and type III interferons, was consistently blunted across all model systems and linked to a highly oxidative, pro-inflammatory environment. This interferon-deficient state altered the host microenvironment in which influenza replicated, spread, and caused disease. Due to the inherent mutability and segmented nature of influenza virus, this altered microenvironment also had implications for the genetic drift and shift of the viral population. Compared to infection of lean hosts, minor variants rapidly emerged within the viral population in obese hosts which exhibited increased viral replication, enhanced virulence, and antiviral resistance. The delayed action of interferon also permitted greater rates of reassortment. Together, this suggested obesity permitted the emergence of novel, and potentially pathogenic, viral variants. Influenza viruses pose constant seasonal and pandemic threats with high morbidity and mortality suffered by overweight and obese patients. If current trends continue, nearly half of the worldwide population will be obese by 2050. This population will have growing impacts on both non-communicable and communicable diseases and may affect global evolutionary trends of influenza virus.

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