Synthetic mRNA capping
2017 ◽
Vol 13
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pp. 2819-2832
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Keyword(s):
Eukaryotic mRNA with its 5′-cap is of central importance for the cell. Many studies involving mRNA require reliable preparation and modification of 5′-capped RNAs. Depending on the length of the desired capped RNA, chemical or enzymatic preparation – or a combination of both – can be advantageous. We review state-of-the art methods and give directions for choosing the appropriate approach. We also discuss the preparation and properties of mRNAs with non-natural caps providing novel features such as improved stability or enhanced translational efficiency.
Keyword(s):
Keyword(s):
2019 ◽
Vol 158
◽
pp. 151-164
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2015 ◽
Vol 91
◽
pp. 91-100
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2015 ◽
Vol 6
(4)
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pp. 410-430
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Keyword(s):
2021 ◽
Vol 17
(4)
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pp. 1-22