scholarly journals Preparation and Application of Multi-functional His-AA-AuNCs Fluorescent Probe

2020 ◽  
Author(s):  
Shaoguang Li ◽  
Jiasong Cai ◽  
Yuan Zhang ◽  
Peiying Shi ◽  
Bing Chen ◽  
...  
Keyword(s):  
Amino Acids ◽  
Hydrogen Peroxide ◽  
Metal Ions ◽  
Fluorescent Probe ◽  
Colorimetric Detection ◽  
Standard Curve ◽  
Characteristic Absorption Peak ◽  
Probe Concentration ◽  
Other Substances ◽  
Promising Application

Functional nanomaterials with simulated properties have become promising candidates for the detection of hydrogen peroxide. However, there are few studies on the colorimetric detection of metal ions and amino acids based on the peroxidase-simulation activity of amino acid functionalized AuNCs. In this study, a method for preparing fluorescent probe using histidine (His) and ascorbic acid (AA) as reductant and stabilizer was proposed. TMB was used as chromogenic substrate to indicate the catalytic process. The ultraviolet absorbance of oxTMB was determined at the characteristic absorption peak 625nm, and the standard curve was drawn to determine the concentration of H2O2 accurately. We found that Fe3+ can greatly improve the response signal of His-AA-AuNCs and has high selectivity. The linear range of H2O2 concentration detection is 10-9.97×106 μM. The concentration range of probe response to Fe3+ is 0.28-280 nM. The His-AA-AuNCs fluorescent probe was applied to intracellular fluorescence imaging after adriamycin injury, and the fluorescence intensity increased with the increase of probe concentration. This study was based on the double-stranded nature of amino acids and the properties of hydrogen peroxide mimic enzymes to detect other substances, which has a promising application prospect, and may potentially be applied to metal ions, amino acids and peptides in the biological and environmental fields in the future.

A novel fluorescent probe for Hg2+ detection in a wide pH range and its application in living cell imaging

2018 ◽  
Vol 10 (46) ◽  
pp. 5554-5558 ◽  
Author(s):  
Jing Xu ◽  
Honglin Li ◽  
Yanchi Chen ◽  
Bing Yang ◽  
Qingcai Jiao ◽  
...  
Keyword(s):  
Amino Acids ◽  
Metal Ions ◽  
Fluorescent Probe ◽  
Living Cell ◽  
Cell Imaging ◽  
Enhanced Fluorescence ◽  
Wide Ph Range ◽  
Ph Range ◽  
Living Cell Imaging

A novel fluorescent probe (XL-1) exhibited over 50-fold enhanced fluorescence in the presence of Hg2+ and no interference could be produced by other metal ions, anions and amino acids.

Highly sensitive colorimetric detection of HgII and CuII in aqueous solutions: from amino acids toward solid platforms

The Analyst ◽  
2015 ◽  
Vol 140 (3) ◽  
pp. 744-749 ◽  
Author(s):  
Jooyoung Park ◽  
Byunggyu In ◽  
Lok Nath Neupane ◽  
Keun-Hyeung Lee
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Aqueous Solutions ◽  
Metal Ions ◽  
Colorimetric Detection ◽  
Selective Detection ◽  
Highly Sensitive ◽  
Fluorescence Change

A chemosensor (NBD-H) based on an amino acid with 7-nitro-2,1,3-benzoxadiazole was used for selective detection of HgII and CuII among 15 metal ions in aqueous solutions by a colorimetric change and fluorescence change.

Promotion of stipe elongation in isolated Flammulina velutipes fruit bodies by carbohydrates, natural extracts, and amino acids

1972 ◽  
Vol 50 (4) ◽  
pp. 803-818 ◽  
Author(s):  
Hans E. Gruen ◽  
Sheue-heng Wu
Keyword(s):  
Amino Acids ◽  
Growth Promotion ◽  
Casein Hydrolysate ◽  
Growth Period ◽  
Flammulina Velutipes ◽  
Ammonium Tartrate ◽  
Acid Growth ◽  
Natural Extracts ◽  
Stipe Elongation ◽  
Other Substances

Isolated Flammulina velutipes fruit bodies were cultured under sterile conditions with the cut base immersed in water or solutions. Stipe elongation on water was only 6% of normal for fruit bodies isolated at 1.1–2.0 cm length, 19% at 5.1–6.0 cm, and the same as for fruit bodies attached to mycelium at 9.1–10.0 cm. Fruit bodies not immersed in water grew less in a saturated atmosphere than those in water. The mycelium must supply other substances than water for normal elongation during about two-thirds of the growth period, and only water thereafter. Isolated fruit bodies fed with filtered glucose, trehalose, sucrose, or mannitol grew better than on water. Maltose and fructose increased elongation only slightly, and sorbose had no effect. Potato extract, yeast extract, and casein hydrolysate gave no or very little growth promotion, but addition of glucose strongly increased growth on the natural extracts compared to glucose alone. Of 21 amino acids added separately to glucose, only asparagine, hydroxyproline, arginine, and to a lesser extent glutamine, stimulated growth of isolated fruit bodies. Growth was not promoted by pure asparagine, glutamine, and serine, or by thiamin or indoleacetic acid. Growth was inhibited by urea, ammonium nitrate, and ammonium tartrate with or without glucose.Growth promoting substances were most effective in young fruit bodies and except for glucose the promotion disappeared in fruit bodies isolated at 6.1–7.0 cm length, which corresponds to the end of the period of rapid elongation. Apical portions of fruit bodies with caps grew better on glucose than whole fruit bodies. Growth of decapitated isolated stipes was not promoted by nutrients.

Carbon–nitrogen conjugate-composited Cu1.8S with enhanced peroxidase-like activity for the colorimetric detection of hydrogen peroxide and glutathione

2021 ◽  
Vol 13 (14) ◽  
pp. 1706-1714
Author(s):  
Nianlu Li ◽  
Mingquan Zhu ◽  
Zhenyu Feng ◽  
Wenhui Lu ◽  
Jing Chen ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Colorimetric Detection ◽  
Colorimetric Analysis ◽  
Higher Sensitivity

In this work, cystine–glucose Maillard conjugates were composited with Cu1.8S microspheres (Cu1.8S–cgmc) to achieve higher sensitivity for the colorimetric analysis.

Study on Adsorption of Crosslinked Starch Microspheres towards Heavy Metal Ions

2013 ◽  
Vol 834-836 ◽  
pp. 531-535
Author(s):  
Li Yan Yang ◽  
Yi Hui Guo ◽  
Li Li Yu ◽  
Jing You
Keyword(s):  
Metal Ions ◽  
Reversed Phase ◽  
Adsorption Rate ◽  
X Ray Diffraction ◽  
Adsorption Selectivity ◽  
X Ray ◽  
Starch Microsphere ◽  
Other Substances ◽  
Effects Of Media ◽  
Kinetics Of

A type of cross-linking starch microsphere (CSMs) has been synthesized via reversed phase suspension method. Crosslinked starch microsphere has good adsorption performance to metal ions in water. The adsorption kinetics of Co (II) on the CSMs, selectivity of adsorption CSMs towards Co (II),Cu (II),Pb (II),Cd (II) and adsorption effects of media towards Co (II) were investigated. The CSMs and its adsorption product were comparatively characterized by X-ray diffraction (XRD). The results showed that The adsorption rate is mainly controlled by liquid film diffusion, and the constant of adsorption rate is 0.0686min-1 at 308K. The crystal structure of the CSMs decreased greatly after the incorporation of Co (II). Co (II) has better adsorption selectivity on CSMs. Ions coexist and other substances in the solution have certain impact on adsorption. Those data are helpful for treatment of the wastewater containing heavy ions.

Development of a coumarin-based fluorescent probe for hydrogen peroxide based on the Payne/Dakin tandem reaction

2021 ◽  
Vol 190 ◽  
pp. 109335
Author(s):  
Fangfang Wu ◽  
Hanjie Yu ◽  
Qin Wang ◽  
Jianjian Zhang ◽  
Zheng Li ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Fluorescent Probe ◽  
Tandem Reaction
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