Promotion of stipe elongation in isolated Flammulina velutipes fruit bodies by carbohydrates, natural extracts, and amino acids

1972 ◽  
Vol 50 (4) ◽  
pp. 803-818 ◽  
Author(s):  
Hans E. Gruen ◽  
Sheue-heng Wu
Keyword(s):  
Amino Acids ◽  
Growth Promotion ◽  
Casein Hydrolysate ◽  
Growth Period ◽  
Flammulina Velutipes ◽  
Ammonium Tartrate ◽  
Acid Growth ◽  
Natural Extracts ◽  
Stipe Elongation ◽  
Other Substances

Isolated Flammulina velutipes fruit bodies were cultured under sterile conditions with the cut base immersed in water or solutions. Stipe elongation on water was only 6% of normal for fruit bodies isolated at 1.1–2.0 cm length, 19% at 5.1–6.0 cm, and the same as for fruit bodies attached to mycelium at 9.1–10.0 cm. Fruit bodies not immersed in water grew less in a saturated atmosphere than those in water. The mycelium must supply other substances than water for normal elongation during about two-thirds of the growth period, and only water thereafter. Isolated fruit bodies fed with filtered glucose, trehalose, sucrose, or mannitol grew better than on water. Maltose and fructose increased elongation only slightly, and sorbose had no effect. Potato extract, yeast extract, and casein hydrolysate gave no or very little growth promotion, but addition of glucose strongly increased growth on the natural extracts compared to glucose alone. Of 21 amino acids added separately to glucose, only asparagine, hydroxyproline, arginine, and to a lesser extent glutamine, stimulated growth of isolated fruit bodies. Growth was not promoted by pure asparagine, glutamine, and serine, or by thiamin or indoleacetic acid. Growth was inhibited by urea, ammonium nitrate, and ammonium tartrate with or without glucose.Growth promoting substances were most effective in young fruit bodies and except for glucose the promotion disappeared in fruit bodies isolated at 6.1–7.0 cm length, which corresponds to the end of the period of rapid elongation. Apical portions of fruit bodies with caps grew better on glucose than whole fruit bodies. Growth of decapitated isolated stipes was not promoted by nutrients.

Promotion of stipe elongation in Flammulina velutipes by a diffusate from excised lamellae supplied with nutrients

1976 ◽  
Vol 54 (12) ◽  
pp. 1306-1315 ◽  
Author(s):  
Hans E. Gruen
Keyword(s):  
Populus Tremuloides ◽  
Growth Promotion ◽  
Early Stage ◽  
Fruit Body ◽  
Flammulina Velutipes ◽  
Malt Extract ◽  
Limited Growth ◽  
Stipe Elongation ◽  
Growth Promoting ◽  
Plain Agar

Flammulina velutipes fruit bodies were grown on partly decayed Populus tremuloides sawdust supplemented with wheat bran and malt extract. In each culture there was a gradation in fruit body size, which served to select test specimens at an early stage of growth. Diffusates collected in agar blocks were applied on the apex of decapitated stipes. Plain agar and dilute potato dextrose agar (PDA/2) alone had the same slight effect on growth. Lamellae placed on plain agar caused limited growth promotion. Lamellae on PDA/2 gave 100–150% more growth promotion than on plain agar during early development, but the activity decreased to zero during the middle of the stage of rapid elongation. Lamellae of that age had no effect on young stipes and the older stipes were insensitive to diffusate from young lamellae. Very small amounts of lamellae promoted stipe elongation. Potato extract alone did not stimulate production of the lamellar diffusate and glucose was less effective than the two nutrients combined. A delay of 2 h in applying lamellar diffusate reduced stipe elongation, and there was no response after 12 h delay. Pilear trama did not produce growth-promoting diffusate.

Control of rapid stipe elongation by the lamellae in fruit bodies of Flammulina velutipes

1979 ◽  
Vol 57 (10) ◽  
pp. 1131-1135 ◽  
Author(s):  
Hans E. Gruen
Keyword(s):  
Growth Period ◽  
Normal Growth ◽  
Flammulina Velutipes ◽  
Stipe Elongation ◽  
Different Ages ◽  
Glucose Agar ◽  
Final Length ◽  
Growth Promoting ◽  
Rapid Elongation

Flammulina velutipes fruit bodies only grew normally if the pileus remained on the stipe until the end of rapid elongation when about 80% of the final length had been reached. During the same period lamellae alone increased stipe growth significantly compared with total decapitation. The stipes attained three-quarters of the length of intact fruit bodies with only about one-third of all lamellae. Near the end of the normal growth period, the stipe became independent of the pileus and lamellae. Small amounts of lamellae excised during rapid elongation produced diffusate in dilute potato glucose agar which promoted growth of decapitated stipes of the same age. The activity could be detected when fruit bodies had completed two-thirds of their normal growth but was much less than at the onset of rapid elongation. Tests of diffusates from young and old lamellae on stipes of different ages showed that the declining influence of lamellae in aging fruit bodies is associated both with decreasing production of the growth-promoting factor and decreasing response of stipes to an external supply of active diffusate. Neither pilear context nor any part of young or old stipes released growth-promoting diffusate.

Protection of Italian ryegrass (Lolium multiflorum L.) seedlings from salinity stress following seed priming with L-methionine and casein hydrolysate

2020 ◽  
pp. 1-9
Author(s):  
Keum-Ah Lee ◽  
Youngnam Kim ◽  
Hossein Alizadeh ◽  
David W.M. Leung
Keyword(s):  
Oxidative Stress ◽  
Amino Acids ◽  
Salinity Stress ◽  
Lolium Multiflorum ◽  
Casein Hydrolysate ◽  
Seed Priming ◽  
Germination Percentage ◽  
Italian Ryegrass ◽  
Significant Difference ◽  
Protein Amino Acids

Abstract Seed priming with water (hydropriming or HP) has been shown to be beneficial for seed germination and plant growth. However, there is little information on the effects of seed priming with amino acids and casein hydrolysate (CH) compared with HP, particularly in relation to early post-germinative seedling growth under salinity stress. In this study, Italian ryegrass seeds (Lolium multiflorum L.) were primed with 1 mM of each of the 20 protein amino acids and CH (200 mg l−1) before they were germinated in 0, 60 and 90 mM NaCl in Petri dishes for 4 d in darkness. Germination percentage (GP), radicle length (RL) and peroxidase (POD) activity in the root of 4-d-old Italian ryegrass seedlings were investigated. Generally, when the seeds were germinated in 0, 60 and 90 mM NaCl, there was no significant difference in GP of seeds among various priming treatments, except that a higher GP was observed in seeds of HP treatment compared with the non-primed seeds when incubated in 60 mM NaCl. When incubated in 60 and 90 mM NaCl, seedlings from seeds primed with L-methionine or CH exhibited greater RL (greater protection against salinity stress) and higher root POD activity than those from non-primed and hydro-primed seeds. Under salinity stress, there were higher levels of malondialdehyde (MDA) in the root of 4-d-old Italian ryegrass seedlings, a marker of oxidative stress, but seed priming with CH was effective in reducing the salinity-triggered increase in MDA content. These results suggest that priming with L-methionine or CH would be better than HP for the protection of seedling root growth under salinity stress and might be associated with enhanced antioxidative defence against salinity-induced oxidative stress.

Technology for the production of frozen zeppelin

2021 ◽  
pp. 16-21
Author(s):  
Владимир Алексеевич Дегтярев ◽  
Владимир Владимирович Литвяк ◽  
Анастасия Владимировна Семенова ◽  
Лидия Борисовна Кузина ◽  
Анастасия Александровна Морозова
Keyword(s):  
Amino Acids ◽  
Fatty Acids ◽  
Free Amino ◽  
Caloric Content ◽  
Pork Meat ◽  
Potato Tubers ◽  
Potato Processing ◽  
Processing Industry ◽  
Other Substances ◽  
Mashed Potatoes

Подробно описана технология получения цеппелинов быстрозамороженных, состоящая из приемки и мойки картофеля, инспекции I и очистки (механической или паровой) картофеля, доочистки и инспекции II картофеля, промывки очищенного картофеля, измельчения клубней картофеля, подготовки натурального картофельного пюре, подготовки компонентов (сухих компонентов, лука, риса), подготовки массы для оболочки цеппелинов, подготовка начинки, формования и замораживания цеппелинов, упаковки и маркировки цеппелинов замороженных, а также транспортирования цеппелинов замороженных потребителю или на склад готовой продукции для хранения. Показано, что цеппелины с мясом свинины содержат белки (3,1 %), жиры (6,9 %), углеводы (14,8 %), органические кислоты (0,2 %), пищевые волокна (1,3 %), золу (1,04 %) и воду (70,4 %), витамины (А, В, В, В, В, В, В, В, Е, D, H, K, PP и др.), макроэлементы (К, Са, Mg, Na, S, P, Cl), микроэлементы, свободные аминокислоты (незаменимые и заменимые), свободные жирные кислоты (насыщенные, мононенасыщенные и полиненасыщенные) и другие вещества, калорийность 133,94 кКал. Технология получения цеппелинов замороженных перспективна для картофелеперерабатывающей отрасли России. The technology for obtaining quick-frozen zeppelin is described in detail, consisting of: acceptance and washing of potatoes, inspection I and cleaning (mechanical or steam) potatoes, additional cleaning and inspection of II potatoes, washing peeled potatoes, grinding potato tubers, preparing natural mashed potatoes, preparing components (dry components, onion, rice), preparation of mass for the shell of zeppelin, preparation of filling, molding and freezing of zeppelin, packaging and labeling of frozen zeppelin, as well as transportation of frozen zeppelin to the consumer or to a finished product warehouse for storage. It has been shown that zeppelins with pork meat contain proteins (3,1 %), fats (6,9 %), carbohydrates (14,8 %), organic acids (0,2 %), dietary fiber (1,3 %), ash (1,04 %) and water (70,4 %), vitamins (A, B, B, B, B, B, B, B, E, D, H, K, PP, etc.), macronutrients ( K, Ca, Mg, Na, S, P, Cl), trace elements, free amino acids (essential and nonessential), free fatty acids (saturated, monounsaturated and polyunsaturated) and other substances, caloric content - 133,94 kcal. The technology for obtaining frozen zeppelin is promising for the potato processing industry in Russia.

Effect of cultural conditions on the synthesis of violacein in mesophilic and psychrophilic strains of Chromobacterium

1969 ◽  
Vol 15 (1) ◽  
pp. 111-116 ◽  
Author(s):  
Kenneth E. Kimmel ◽  
Siegfried Maier
Keyword(s):  
Amino Acids ◽  
Cell Mass ◽  
Casein Hydrolysate ◽  
Pigment Production ◽  
Complex Media ◽  
Purple Pigment ◽  
Facultatively Anaerobic ◽  
Cultural Conditions ◽  
Simple Medium

A stimulatory function of tryptophan was indicated in the synthesis of the purple pigment, violacein, in nine strains of Chromobacterium. In complex media, tryptophan increased the production of pigment, but the addition of glucose to a complex tryptophan medium supported considerably more pigmentation/cell mass. In a simple medium such as Koser citrate broth, tryptophan was essential for pigment production; however, pigment was produced in casein hydrolysate, indicating that other amino acids could replace the tryptophan requirement. Both mesophilic and psychrophilic strains were facultatively anaerobic in growth but required oxygen for pigmentation.

scholarly journals Co-Inoculation of Bacillus velezensis Strain S141 and Bradyrhizobium Strains Promotes Nodule Growth and Nitrogen Fixation

2020 ◽  
Vol 8 (5) ◽  
pp. 678
Author(s):  
Surachat Sibponkrung ◽  
Takahiko Kondo ◽  
Kosei Tanaka ◽  
Panlada Tittabutr ◽  
Nantakorn Boonkerd ◽  
...  
Keyword(s):  
N2 Fixation ◽  
Growth Promotion ◽  
Nodule Formation ◽  
Bacillus Velezensis ◽  
Cytokinin Biosynthesis ◽  
Large Size ◽  
Other Substances ◽  
Glycine Max L ◽  
Iaa Biosynthesis

The objective of this research was to evaluate the PGPR effect on nodulation and nitrogen-fixing efficiency of soybean (Glycine max (L.) Merr.) by co-inoculation with Bradyrhizobium diazoefficiens USDA110. Co-inoculation of Bacillus velezensis S141 with USDA110 into soybean resulted in enhanced nodulation and N2-fixing efficiency by producing larger nodules. To understand the role of S141 on soybean and USDA110 symbiosis, putative genes related to IAA biosynthesis were disrupted, suggesting that co-inoculation of USDA110 with S141ΔyhcX reduces the number of large size nodules. It was revealed that yhcX may play a major role in IAA biosynthesis in S141 as well as provide a major impact on soybean growth promotion. The disruption of genes related to cytokinin biosynthesis and co-inoculation of USDA110 with S141ΔIPI reduced the number of very large size nodules, and it appears that IPI might play an important role in nodule size of soybean–Bradyrhizobium symbiosis. However, it was possible that not only IAA and cytokinin but also some other substances secreted from S141 facilitate Bradyrhizobium to trigger bigger nodule formation, resulting in enhanced N2-fixation. Therefore, the ability of S141 with Bradyrhizobium co-inoculation to enhance soybean N2-fixation strategy could be further developed for supreme soybean inoculants.

scholarly journals Potential Benefits of Ameliorating Metabolic and Nutritional Abnormalities in People With Profound Developmental Disabilities

2017 ◽  
Vol 10 ◽  
pp. 117863881771645
Author(s):  
Norris R Glick ◽  
Milton H Fischer
Keyword(s):  
Quality Of Life ◽  
Oxidative Stress ◽  
Amino Acids ◽  
Developmental Disabilities ◽  
Mead Acid ◽  
Other Substances ◽  
Adrenic Acid ◽  
Potential Benefits ◽  
Cell Implantation

Background: People with profound developmental disabilities have some of the most severe neurological impairments seen in society, have accelerated mortality due to huge medical challenges, and yet are often excluded from scientific studies. They actually have at least 2 layers of conditions: (1) the original disability and (2) multiple under-recognized and underexplored metabolic and nutritional imbalances involving minerals (calcium, zinc, and selenium), amino acids (taurine, tryptophan), fatty acids (linoleic acid, docosahexaenoic acid, arachidonic acid, adrenic acid, Mead acid, plasmalogens), carnitine, hormones (insulinlike growth factor 1), measures of oxidative stress, and likely other substances and systems. Summary: This review provides the first list of metabolic and nutritional abnormalities commonly found in people with profound developmental disabilities and, based on the quality of life effects of similar abnormalities in neurotypical people, indicates the potential effects of these abnormalities in this population which often cannot communicate symptoms. Key messages: We propose that improved understanding and management of these disturbed mechanisms would enhance the quality of life of people with profound developmental disabilities. Such insights may also apply to people with other conditions associated with disability, including some diseases requiring stem cell implantation and living in microgravity.

Studies on the Nutrition of Marine Flatfish the Essential Amino Acid Requirements of Plaice and Sole

1970 ◽  
Vol 50 (1) ◽  
pp. 87-95 ◽  
Author(s):  
C. B. Cowey ◽  
J. Adron ◽  
A. Blair
Keyword(s):  
Amino Acids ◽  
Essential Amino Acid ◽  
Nutritional Value ◽  
Essential Amino Acids ◽  
Present Knowledge ◽  
Radioactive Carbon ◽  
Dietary Requirement ◽  
Fish Diets ◽  
Other Substances ◽  
Amino Acid Requirements

Present knowledge of the nutritional requirements of fish is limited to certain species of salmon and trout (e.g. Halver, DeLong & Mertz, 1957; Shanks, Gahimer & Halver, 1962). In view of the current interest in the cultivation of marine flatfish (Shelbourne, 1964) information on their dietary needs is equally desirable. Experience with salmon (DeLong, Halver & Mertz, 1958) has indicated that protein is the largest and most expensive constituent of fish diets and studies on the nutritional value of different food proteins for fish are necessary if the fish are to be reared economically and successfully. A desirable prerequisite to such studies is a knowledge of those amino acids for which the fish has an absolute dietary requirement, i.e. which it cannot itself synthesize from other substances normally available. With most animals these requirements have been identified by growth trials using partially (or wholly) defined diets. The rearing of fish on defined diets is not easy and it seemed in the first instance that an indirect but valid approach might be utilized. If animals are given a radioactively labelled carbon source, such as uniformly labelled [U-14C]glucose a significant amount of the radioactive carbon will be incorporated only into those compounds which the animal can itself synthesize. By isolating the amino acids from the tissue proteins following such a dose the essential amino acids are identified as those which are not radioactive. The amino acids containing radioactivity correspond with the so-called non-essential amino acids.

scholarly journals Sorption of Amino Acids and Other Substances by Amberlite Ion Exchange Resins1

1954 ◽  
Vol 22 (4) ◽  
pp. 349-355 ◽  
Author(s):  
Francis M Thurmon ◽  
Bertha Ottenstein ◽  
Nicholas F Boncoddo ◽  
Maurice J Bessman
Keyword(s):  
Amino Acids ◽  
Ion Exchange ◽  
Other Substances
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