NANOEMULSIONS BASED ON LIPOIC ACID DERIVATIVES WITH VARIOUS ALCOHOLS: PRODUCTION AND INFLUENCE ON FUNCTIONAL ACTIVITY OF PLATELETS

2020 ◽  
pp. 461-464
Author(s):  
A. Sinebryukhova ◽  
A. Shipelova ◽  
E. Darnotuk ◽  
A. Chekanov ◽  
O. Baranova ◽  
...  
Keyword(s):  
Particle Size ◽  
Arachidonic Acid ◽  
Lipoic Acid ◽  
Encapsulation Efficiency ◽  
Room Temperature ◽  
Optimal Conditions ◽  
Pluronic F68 ◽  
Long Term Storage ◽  
Term Storage

The optimal conditions were selected for obtaining homogeneous nanoemulsions (NE) of lipoic acid conjugates (LA-conjugates) based on Pluronic F68 (1,8%) with a particle size not exceeding 400 nm, characterized by 97±2% encapsulation efficiency of substances in nanoparticles (NP). A heterogeneous NE (polydispersity index, PDI>0,3) with the derivative of LA and myo-inositol based on phosphatidylcholine (PC, C = 3 mg/ml) was also obtained consisting of 2 particle fractions: 20–70 nm (27%) and 122–212 nm (73%). The obtained NEs with LA-conjugates based on Pluronic F68 and PC were stable during long-term storage (more than 12 months) at room temperature. The effect of the obtained NEs of LA-conjugates on platelet aggregation (Pt) caused by arachidonic acid (AA) was determined, and a mechanism of their action was proposed.

scholarly journals HMGB1 concentration measurements in trauma patients: assessment of pre-analytical conditions and sample material

2019 ◽  
Vol 26 (1) ◽  
Author(s):  
William Ottestad ◽  
Ingrid N. Rognes ◽  
Erlend Skaga ◽  
Cassandra Frisvoll ◽  
Guttorm Haraldsen ◽  
...  
Keyword(s):  
Western Blot ◽  
Room Temperature ◽  
Trauma Patients ◽  
Plasma Samples ◽  
Sample Material ◽  
Limits Of Agreement ◽  
Freeze Thaw ◽  
Long Term Storage ◽  
Term Storage

Abstract Background HMGB1 is a mediator of systemic inflammation in sepsis and trauma, and a promising biomarker in many diseases. There is currently no standard operating procedure for pre-analytical handling of HMGB1 samples, despite that pre-analytical conditions account for a substantial part of the overall error rate in laboratory testing. We hypothesized that the considerable variations in reported HMGB1 concentrations and kinetics in trauma patients could be partly explained by differences in pre-analytical conditions and choice of sample material. Methods Trauma patients (n = 21) admitted to a Norwegian Level I trauma center were prospectively included. Blood was drawn in K2EDTA coated tubes and serum tubes. The effects of delayed centrifugation were evaluated in samples stored at room temperature for 15 min, 3, 6, 12, and 24 h respectively. Plasma samples subjected to long-term storage in − 80 °C and to repeated freeze/thaw cycles were compared with previously analyzed samples. HMGB1 concentrations in simultaneously acquired arterial and venous samples were also compared. HMGB1 was assessed by standard ELISA technique, additionally we investigated the suitability of western blot in both serum and plasma samples. Results Arterial HMGB1 concentrations were consistently lower than venous concentrations in simultaneously obtained samples (arterial = 0.60 x venous; 95% CI 0.30–0.90). Concentrations in plasma and serum showed a strong linear correlation, however wide limits of agreement. Storage of blood samples at room temperature prior to centrifugation resulted in an exponential increase in plasma concentrations after ≈6 h. HMGB1 concentrations were fairly stable in centrifuged plasma samples subjected to long-term storage and freeze/thaw cycles. We were not able to detect HMGB1 in either serum or plasma from our trauma patients using western blotting. Conclusions Arterial and venous HMGB1 concentrations cannot be directly compared, and concentration values in plasma and serum must be compared with caution due to wide limits of agreement. Although HMGB1 levels in clinical samples from trauma patients are fairly stable, strict adherence to a pre-analytical protocol is advisable in order to protect sample integrity. Surprisingly, we were unable to detect HMGB1 utilizing standard western blot analysis.

Long-Term Microhardness Evolution in Ti-Ni Shape Memory Alloys Processed by Severe Cold Rolling

2008 ◽  
Vol 584-586 ◽  
pp. 1039-1044
Author(s):  
Andrey Korotitskiy ◽  
K.E. Inaekyan ◽  
Vladimir Brailovski ◽  
Sergey Prokoshkin
Keyword(s):  
Cold Rolling ◽  
Shape Memory ◽  
Room Temperature ◽  
Structural Changes ◽  
Vickers Microhardness ◽  
Time Dependent ◽  
Logarithmic Strain ◽  
Long Term Storage ◽  
Term Storage

Ti-50.26at.%Ni shape memory alloy samples were subjected to cold rolling (CR) with true strains encompassing from moderate (logarithmic strain e=0.25) to severe (e=2.1) deformation. СR with e = 0.5 and more initiated a partial austenite amorphization. The evaluation of structural changes in the material during its long-term storage was performed using Vickers microhardness (HV) technique. It was shown that during storage at room temperature up to 9 months, microhardness varied following a dome-shaped trend, thus reflecting commonly encountered interaction between two concurrent time-dependent phenomena, the first responsible for the material hardening, and the second, for the material softening. To represent such phenomena, a simple mathematical model was proposed and experimentally validated.

scholarly journals Development of cryopreservation methods of seed of Nepeta cataria

2021 ◽  
Vol 102 (2) ◽  
pp. 37-42
Author(s):  
Margarita Ishmuratova ◽  
◽  
Damirzhan Baigarayev ◽  
Saltanat Tleukenova ◽  
Elena Gavrilkova ◽  
...  
Keyword(s):  
Liquid Nitrogen ◽  
Room Temperature ◽  
Seed Viability ◽  
Positive Temperature ◽  
Seed Rate ◽  
Nepeta Cataria ◽  
Long Term Storage ◽  
Medical Plant ◽  
Term Storage

This article presents the summarized data on cryopreservation of seeds of the medical plant Nepeta cataria. Cryopreservation is a highly promising method for saving of seed materials, allowing to organize long-term storage without viability loss. The purpose of present work is to optimize conditions of cryopreservation of seed materials of Nepeta cataria. Assessment of seed survival rate in the storage showed a linear decrease in seed viability and energy of germination. After 30 months of storage at the low positive temperature (+5 ºC) in paper pack seed rate decreased to 12.0 % and energy of germination to 11.2 %; after 4 years of storage seeds lost viability. During conduction of research the type of container, condition of thawing, optimal moisture of seeds and cryoprotectants are optimized. The optimal container for cryopreservation in liquid nitrogen was plastic cryo tubes; defrosting at room temperature. The best seed rate is found at moisture 3 %; the best cryoprotectant was glucose, the optimal concentration was 15 %. The result of the research is used for creation of the long-term storage medicinal cultures’ seed bank in the liquid nitrogen.

scholarly journals Changes of salivary biomarkers under different storage conditions: effects of temperature and length of storage

2018 ◽  
Vol 29 (1) ◽  
pp. 94-111 ◽  
Author(s):  
Tomás Barranco ◽  
Asta Tvarijonaviciute ◽  
Damián Escribano ◽  
Fernando Tecles ◽  
José J Cerón ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Room Temperature ◽  
Storage Conditions ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Long Term Storage ◽  
Reducing Ability ◽  
Trolox Equivalent ◽  
The Stability ◽  
Term Storage

Introduction: In this report, we aimed to examine the stability of various analytes in saliva under different storage conditions. Materials and methods: Alpha-amylase (AMY), cholinesterase (CHE), lipase (Lip), total esterase (TEA), creatine kinase (CK), aspartate aminotransferase (AST), lactate dehydrogenase (LD), lactate (Lact), adenosine deaminase (ADA), Trolox equivalent antioxidant capacity (TEAC), ferric reducing ability (FRAS), cupric reducing antioxidant capacity (CUPRAC), uric acid (UA), catalase (CAT), advanced oxidation protein products (AOPP) and hydrogen peroxide (H2O2) were colorimetrically measured in saliva obtained by passive drool from 12 healthy voluntary donors at baseline and after 3, 6, 24, 72 hours, 7 and 14 days at room temperature (RT) and 4 ºC, and after 14 days, 1, 3 and 6 months at – 20 ºC and – 80 ºC. Results: At RT, changes appeared at 6 hours for TEA and H2O2; 24 hours for Lip, CK, ADA and CUPRAC; and 72 hours for LD, Lact, FRAS, UA and AOPP. At 4 ºC changes were observed after 6 hours for TEA and H2O2; 24 hours for Lip and CUPRAC; 72 hours for CK; and 7 days for LD, FRAS and UA. At – 20 ºC changes appeared after 14 days for AST, Lip, CK and LD; and 3 months for TEA and H2O2. At – 80 ºC observed changes were after 3 months for TEA and H2O2. Conclusions: In short-term storage, the analytes were more stable at 4 ºC than at room temperature, whereas in long-term storage they were more stable at - 80 ºC than at – 20 ºC.

Long-Term Storage of Salivary Cortisol Samples at Room Temperature

1992 ◽  
Vol 38 (2) ◽  
pp. 304-304 ◽  
Author(s):  
Yu-Ming Chen ◽  
Nitza M Cintrón ◽  
Peggy A Whitson
Keyword(s):  
Salivary Cortisol ◽  
Room Temperature ◽  
Long Term Storage ◽  
Term Storage

Long-term room temperature storage of DNA molecules

Biomics ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 552-563
Author(s):  
R.R. Garafutdinov ◽  
A.R. Sakhabutdinova ◽  
A.V. Chemeris
Keyword(s):  
Data Storage ◽  
Room Temperature ◽  
Biological Data ◽  
Dna Molecules ◽  
Freezing And Thawing ◽  
Long Term Storage ◽  
Wide Range ◽  
Frozen Solutions ◽  
Term Storage

The simplest and most common method of long-term storage of DNA samples at present is the storage of their frozen solutions, which, however, has a number of disadvantages, including the destruction of DNA molecules during freezing and thawing, as well as energy consumption and the likelihood of losing valuable samples in the event of possible accidents. In this regard, long-term storage of DNA samples at room temperature in a dried state is preferable, especially since an even greater increase in the number of stored DNA samples is planned due to the planned preservation of non-biological data in this molecule, which is recognized at the International Economic Forum 2019 among the 10 most important innovative technologies as “DNA Data Storage” of the near future of mankind. Such storage requires the exclusion of hydrolysis and oxidation of DNA molecules under the action of water and reactive oxygen species, which can be achieved by placing DNA in an inert anhydrous atmosphere, including in the presence of additional ingredients in the form of, for example, trehalose, imitating wildlife, since it is known that this simple disaccharide, capable of vitrification, protects a wide range of anhydrobiont organisms from adverse environmental conditions. Currently, there are a number of technologies that provide long-term storage of DNA at room temperature, including those available from commercial sources, but not all problems have yet been solved, which is reflected in this review article.

scholarly journals Rhodamine Fluorescence After 15-year Storage in Methyl Salicylate

2006 ◽  
Vol 14 (2) ◽  
pp. 48-48
Author(s):  
Philip L. Hertzler
Keyword(s):  
Methyl Salicylate ◽  
Laser Scanning ◽  
Room Temperature ◽  
Bacterial Degradation ◽  
Cell Stage ◽  
Long Term Storage ◽  
Central Michigan University ◽  
Dark Figure ◽  
Term Storage

Fading of fluorochrome is a significant limitation to fluorescence microscopy. Several anti-fade agents, e.g. n-propyl gallate, are commonly used for glycerol-based mounting media (Longin et al., 1993; Ono et al., 2001). Samples mounted in glycerol must be kept at -20°C for long-term storage to prevent bacterial degradation. In contrast, fluorescent samples cleared and mounted in organic media can be stored indefinitely at room temperature.Methyl salicylate or oil of wintergreen is an excellent clearing agent (refractive index = 1.53), which works well with a variety of fluorochromes. It has a pleasant aroma but is somewhat difficult to work with since it remains liquid after mounting. It was previously reported that shrimp embryos labeled with tubulin antibody and rhodamine-conjugated secondary antibody maintained their fluorescence after six months (Summers et al. 1993). These same samples, stained in November, 1990 and imaged by confocal microscopy for publication in Hertzler and Clark (1992), are still fluorescent after continuous storage in methyl salicylate at room temperature in the dark (Figure 1). The images of 62-cell stage shrimp embryos taken from these 1990 samples were collected with an Olympus Fluoview 300 laser scanning confocal microscope in January, 2006 in the Dept. of Biology, Central Michigan University.

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