scholarly journals SINTESIS CALKON ((E)-1,3-DI(NAPHTHALEN-2-YL)PROP-2-EN-1-ONE) DAN AKTIVITASNYA SEBAGAI ANTIOKSIDAN

2012 ◽  
Vol 3 (1) ◽  
pp. 7-14
Author(s):  
Rahmiwati Hilma ◽  
Jasril ◽  
Hilwan Yuda Teruna
Keyword(s):  
Free Radical ◽  
Melting Point ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Room Temperature ◽  
Intermediate Compound ◽  
Alkaline Condition ◽  
Point Measurement ◽  
Anti Inflammatory ◽  
Layer Chromatography

Study on chalcone calkon (E)-1,3-di(naphthalen-2-yl)prop-2-en-1-one synthesis have been carried out with stirrer methode. These compounds can be used as intermediate compound to synthesize others compounds which was reported having antimicrobial, anti-inflammatory, anti-depressant, anti-tumour. The of chalcones synthesis vatives were reported in acid and alkali condition. In this study, chalcone and its derivates were synthesized by using stirrer method in alkaline condition in room temperature. the compounds subjected to somes analyses including melting point measurement, thin layer chromatography and HPLC. Scavenging free radical by using DPPH methode showed Scavenging free radical with LC50 >80 μg/ml min potent activity while the ascorbat acid LC50 89,79 μg/ml.

An efficient biotransformation of progesterone into 11α-hydroxyprogesterone by Rhizopus microsporus var. oligosporus

2018 ◽  
Vol 74 (1-2) ◽  
pp. 9-15
Author(s):  
Bahman Nickavar ◽  
Hossein Vahidi ◽  
Mehrnoosh Eslami
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Time Course ◽  
Main Product ◽  
Fermented Foods ◽  
Rhizopus Microsporus ◽  
Chromatographic Methods ◽  
Anti Inflammatory ◽  
Layer Chromatography

Abstract Rhizopus microsporus var. oligosporus is a fungus that belongs to the Mucoraceae family that is used for the preparation of some soy-fermented foods. Microbial biotransformation of progesterone by R. microsporus var. oligosporus afforded some monohydroxylated and dihydroxylated metabolites. The main product was purified using chromatographic methods and identified as 11α-hydroxyprogesterone on the basis of its spectroscopic features. Time course studies by high-performance thin-layer chromatography demonstrated that this fungi efficiently hydroxylated progesterone at the 11α-position for 3 days with a yield of 76.48%, but beyond this time, the microorganism transformed 11α-hydroxyprogesterone into dihydroxylated metabolites. 11α-Hydroxyprogesterone is widely used as a precursor in the synthesis of hydrocortisone and other steroidal anti-inflammatory agents.

Single-reagent polarization fluoroimmunoassay for barbiturates in urine.

1984 ◽  
Vol 30 (11) ◽  
pp. 1765-1769 ◽  
Author(s):  
D L Colbert ◽  
D S Smith ◽  
J Landon ◽  
A M Sidki
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Fluorescence Polarization ◽  
Room Temperature ◽  
Cross Reactivity ◽  
Screening Techniques ◽  
Standard Curve ◽  
Layer Chromatography

Abstract We describe a rapid polarization fluoroimmunoassay for screening for the presence of barbiturates in urine. The single reagent is prepared by pre-mixing antiserum with a fluorescein-labeled barbiturate derivative as tracer. Use of a mixture of two sheep antisera, raised against different barbiturate immunogens, results in an assay with a broad cross-reactivity spectrum for most common barbiturates. One adds urine to the pre-mixed reagent, incubates the solution for a few minutes at room temperature, and measures the fluorescence polarization. The tracer/antiserum reagent is stable for at least six months at 4 degrees C. Results compare favorably with two other commonly used screening techniques for barbiturates, thin-layer chromatography and the EMIT-stTM (Syva) system. Although designed as a qualitative screen, some drugs can be quantified by means of a standard curve of the relevant barbiturate.

Instability of Some TWchothecenes in Methanol

1986 ◽  
Vol 69 (5) ◽  
pp. 902-903 ◽  
Author(s):  
Ru-Dong Wei ◽  
Fun S Chu
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Room Temperature ◽  
Early Stage ◽  
Acetoxy Group ◽  
Layer Chromatography

Abstract Stability of 8 trichothecenes stored in methanol at room temperature was studied by thin layer chromatography. Results indicate that the trichothecenes which bear an acetoxy group at both C3 and C4 are very susceptible to methanolysis. In the early stage, the acetoxy group at C3 is most favorable for the transesterification. All trichothecenes tested were transformed to several products after prolonged (22 days) exposure to methanol.

A PRACTICAL METHOD FOR ESTIMATION OF URINARY PREGNANEDIOL AND ALLOPREGNANEDIOL USING THIN LAYER CHROMATOGRAPHY

1965 ◽  
Vol 49 (1) ◽  
pp. 97-106 ◽  
Author(s):  
S. Sulimovici ◽  
B. Lunenfeld ◽  
M. C. Shelesnyak
Keyword(s):  
Melting Point ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Practical Method ◽  
Melting Point Determination ◽  
Uv Absorption Spectra ◽  
Infra Red ◽  
Point Determination ◽  
Layer Chromatography ◽  
Hydrolysis Of

ABSTRACT A method is described for the assay of urinary pregnanediol and allopregnanediol which employs acid hydrolysis of pregnanediol glucuronide, ascending thin-layer chromatography and spectrophotometric quantitation of the eluted pregnanediol and allopregnanediol. The reliability of the method was established by analysis of infra-red and UV absorption spectra, melting point determination, recovery experiments, duplicate assays and comparison with the Klopper method. The method has advantages in that it is rapid and requires small urine samples.

scholarly journals Fluoroponytailed Ionic Liquids as Co-Porogens for Poly(butyl methacrylate-co-ethylene dimethacrylate) Monolithic Supports for Thin Layer Chromatography

The Analyst ◽  
2022 ◽  
Author(s):  
Manuel Otter ◽  
Gabriel Partl ◽  
Michael Franz Noisternig ◽  
Rania Bakry
Keyword(s):  
Ionic Liquids ◽  
Free Radical ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Radical Polymerization ◽  
Free Radical Polymerization ◽  
Butyl Methacrylate ◽  
Ethylene Dimethacrylate ◽  
Layer Chromatography

Porous layered monolithic substrates of poly(butyl methacrylate-co-ethylene dimethacrylate) were synthesized via UV initiated free radical polymerization in presence of fluoroponytailed ionic liquids as co-porogenic constituents. The effects of the type...

Sterols produced by Cryptotnonas ovata var. palustris

1968 ◽  
Vol 14 (8) ◽  
pp. 837-839 ◽  
Author(s):  
R. P. Collins ◽  
K. Kalnins
Keyword(s):  
Infrared Spectroscopy ◽  
Melting Point ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Mass Spectroscopy ◽  
Layer Chromatography

Cholesterol and stigmasterol were isolated from C. ovata var. palustris. The compounds were identified by thin-layer chromatography, mass spectroscopy, infrared spectroscopy, derivative formation, and melting point determinations.

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