A PRACTICAL METHOD FOR ESTIMATION OF URINARY PREGNANEDIOL AND ALLOPREGNANEDIOL USING THIN LAYER CHROMATOGRAPHY

1965 ◽  
Vol 49 (1) ◽  
pp. 97-106 ◽  
Author(s):  
S. Sulimovici ◽  
B. Lunenfeld ◽  
M. C. Shelesnyak
Keyword(s):  
Melting Point ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Practical Method ◽  
Melting Point Determination ◽  
Uv Absorption Spectra ◽  
Infra Red ◽  
Point Determination ◽  
Layer Chromatography ◽  
Hydrolysis Of

ABSTRACT A method is described for the assay of urinary pregnanediol and allopregnanediol which employs acid hydrolysis of pregnanediol glucuronide, ascending thin-layer chromatography and spectrophotometric quantitation of the eluted pregnanediol and allopregnanediol. The reliability of the method was established by analysis of infra-red and UV absorption spectra, melting point determination, recovery experiments, duplicate assays and comparison with the Klopper method. The method has advantages in that it is rapid and requires small urine samples.

BIOSYNTHESIS OF EQUILIN AND RELATED RING B UNSATURATED OESTROGENS IN PERFUSED HUMAN PLACENTA

1966 ◽  
Vol 34 (4) ◽  
pp. 447-456 ◽  
Author(s):  
L. STÁRKA ◽  
H. BREUER ◽  
L. CEDARD
Keyword(s):  
Melting Point ◽  
Thin Layer ◽  
Human Placenta ◽  
Placental Tissue ◽  
Melting Point Determination ◽  
Pregnancy Urine ◽  
Human Placental Tissue ◽  
Point Determination ◽  
Layer Chromatography ◽  
Steroid Metabolite

SUMMARY Equilin, equilenin, 17β-dihydroequilin and 17β-dihydroequilenin were formed during perfusion of androsta-4,7-diene-3,17-dione, 3β-hydroxyandrosta-5,7-dien-17-one and 3β,7α-dihydroxyandrost-5-en-17-one through human placenta. The ring B unsaturated oestrogens were isolated and identified by paper chromatography, thin-layer chromatography, melting point determination, ultraviolet spectra, infrared spectra and various colour reactions. The present findings show that human placental tissue is capable of forming ring B unsaturated oestrogens from neutral C-19 steroids. Of these, 3β,7α-dihydroxyandrost-5-en-17-one is a normal steroid metabolite in man. It seems, therefore, reasonable to assume that equilin and related ring B unsaturated oestrogens may occur in human pregnancy urine.

scholarly journals ISOLATION AND ANALYSIS OF STEROIDAL SAPONINS FROM POLYGONATUM ODORATUM (MILL.) DRUCE

2021 ◽  
Vol 55 ◽  
pp. 135-140
Author(s):  
Cristina MOGOSAN ◽  
Ilioara ONIGA ◽  
Mircea TAMAS
Keyword(s):  
Thin Layer ◽  
Acid Hydrolysis ◽  
Thin Layer Chromatography ◽  
Biological Properties ◽  
Steroidal Saponins ◽  
Physico Chemical ◽  
Layer Chromatography ◽  
Hydrolysis Of

We isolated the steroidal saponins from the rhizomes of Polygonatum odoratum (Mill.) Druce with an efficiency of 4.50% which represents 7 fractions identified by thin-layer chromatography (TLC), of which 3 were furostanics and 4 spirostanics. After the acid hydrolysis of the saponins, one aglycone (sapogenine) was identified by TLC. Further, we have determined the physico-chemical and the biological properties of the isolated saponins.

scholarly journals SINTESIS CALKON ((E)-1,3-DI(NAPHTHALEN-2-YL)PROP-2-EN-1-ONE) DAN AKTIVITASNYA SEBAGAI ANTIOKSIDAN

2012 ◽  
Vol 3 (1) ◽  
pp. 7-14
Author(s):  
Rahmiwati Hilma ◽  
Jasril ◽  
Hilwan Yuda Teruna
Keyword(s):  
Free Radical ◽  
Melting Point ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Room Temperature ◽  
Intermediate Compound ◽  
Alkaline Condition ◽  
Point Measurement ◽  
Anti Inflammatory ◽  
Layer Chromatography

Study on chalcone calkon (E)-1,3-di(naphthalen-2-yl)prop-2-en-1-one synthesis have been carried out with stirrer methode. These compounds can be used as intermediate compound to synthesize others compounds which was reported having antimicrobial, anti-inflammatory, anti-depressant, anti-tumour. The of chalcones synthesis vatives were reported in acid and alkali condition. In this study, chalcone and its derivates were synthesized by using stirrer method in alkaline condition in room temperature. the compounds subjected to somes analyses including melting point measurement, thin layer chromatography and HPLC. Scavenging free radical by using DPPH methode showed Scavenging free radical with LC50 >80 μg/ml min potent activity while the ascorbat acid LC50 89,79 μg/ml.

Sterols produced by Cryptotnonas ovata var. palustris

1968 ◽  
Vol 14 (8) ◽  
pp. 837-839 ◽  
Author(s):  
R. P. Collins ◽  
K. Kalnins
Keyword(s):  
Infrared Spectroscopy ◽  
Melting Point ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Mass Spectroscopy ◽  
Layer Chromatography

Cholesterol and stigmasterol were isolated from C. ovata var. palustris. The compounds were identified by thin-layer chromatography, mass spectroscopy, infrared spectroscopy, derivative formation, and melting point determinations.

Carbonyl Compounds in Salted Cod.: II. Separation and Identification of Volatile Monocarbonyl Compounds from Heavily Salted Cod

1965 ◽  
Vol 22 (1) ◽  
pp. 27-32 ◽  
Author(s):  
M. Yurkowski ◽  
M.A. Bordeleau
Keyword(s):  
Melting Point ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Chain Length ◽  
Carbonyl Compounds ◽  
Gadus Morhua ◽  
Unknown Compound ◽  
Layer Chromatography

Volatile monocarbonyl compounds were isolated from heavily salted cod (Gadus morhua) as the 2,4-dinitrophenylhydrazones. These compounds were separated by thin-layer chromatography first into classes and then according to chain length. n-Alkanals C1–5 and n-alk-2-enals C5–6 were conclusively identified. n-Alkanals C8,10 and n-alk-2-enals C4,7,8 were tentatively identified. An unknown compound corresponding to n-hexanal, except in the melting point, was isolated. Formaldehyde, and to a lesser extent, acetaldehyde and propanal, were the major monocarbonyls present in salted cod.

scholarly journals Synthesis and characterization of novel substituted spiro[isobenzofuran-1(3H), 9’-xanthene]-3-ones

2005 ◽  
Vol 70 (7) ◽  
pp. 931-936 ◽  
Author(s):  
Sachin.V. Patel ◽  
Manish Patel ◽  
Rangan Patel
Keyword(s):  
Melting Point ◽  
Nmr Spectroscopy ◽  
Benzoic Acid ◽  
Synthesis And Characterization ◽  
Acidic Media ◽  
Melting Point Determination ◽  
Infra Red ◽  
Point Determination ◽  
Uv Visible

The ketoacid, 2-(4-diethylamino-2-hydroxybenzoyl)benzoic acid, prepared from N,N-diethyl-m-aminophenol and phthalic anhydride, was reacted with various substituted 3-[6-methoxybenzothiazol-2-yl]-4(3H)-quinazolinones in the presence of a dehydration condensing agent to afford novel spiro[isobenzofuran-1(3H),9?-xanthene]-3-ones. The benzothiazolyl quinazolinones were synthesized by reacting 2-amino-6-methoxybenzothiazole with various substituted benzoxazinones. All compounds were characterized by melting point determination, elemental analysis, infra- red spectroscopy NMR-spectroscopy and UV-visible sprectroscopy. All the fluoran compounds are colourless or nearly colourless and produce colour in the presence of acidic media.

Sign in / Sign up

Export Citation Format

Share Document