scholarly journals Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

10.3791/55119 ◽  
2017 ◽  
Author(s):  
Frederik Görlitz ◽  
Douglas J. Kelly ◽  
Sean C. Warren ◽  
Dominic Alibhai ◽  
Lucien West ◽  
...  
Keyword(s):  
Content Analysis ◽  
Open Source ◽  
Fluorescence Lifetime ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging ◽  
High Content Analysis

Automated multiwell fluorescence lifetime imaging for Förster resonance energy transfer assays and high content analysis

2015 ◽  
Vol 7 (10) ◽  
pp. 4071-4089 ◽  
Author(s):  
Douglas J. Kelly ◽  
Sean C. Warren ◽  
Dominic Alibhai ◽  
Sunil Kumar ◽  
Yuriy Alexandrov ◽  
...  
Keyword(s):  
Content Analysis ◽  
Energy Transfer ◽  
Fluorescence Lifetime ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Förster Resonance Energy Transfer ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging ◽  
High Content Analysis ◽  
Förster Resonance

An HCA-FLIM instrument is presented alongside exemplar oligomerisation, intermolecular and intramolecular FRET assays that require robust measurement of small lifetime changes.

scholarly journals High speed unsupervised fluorescence lifetime imaging confocal multiwell plate reader for high content analysis

2008 ◽  
Vol 1 (6) ◽  
pp. 514-521 ◽  
Author(s):  
Clifford B. Talbot ◽  
James McGinty ◽  
David M. Grant ◽  
Ewan J. McGhee ◽  
Dylan M. Owen ◽  
...  
Keyword(s):  
Content Analysis ◽  
Fluorescence Lifetime ◽  
High Speed ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging ◽  
Plate Reader ◽  
High Content Analysis

Visualization of Cutaneous Distribution of Minocycline of a Topical Gel in Human Facial Skin with Two-Photon Excited Fluorescence Lifetime Imaging Microscopy (FLIM) and Phasor Analysis

2017 ◽  
Vol 1 ◽  
pp. s57
Author(s):  
Sinyoung Jeong ◽  
Maiko Hermsmeier ◽  
Sam Osseiran ◽  
Akira Yamamoto ◽  
Usha Nagavarapu ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Facial Skin ◽  
Topical Gel ◽  
Two Photon ◽  
Lifetime Imaging ◽  
Phasor Analysis

Abstract Not AvailableStudy supported by BioPharmX.

Effects of Photon Losses on Fluorescence Lifetime Imaging Microscopy (FLIM) System Optimization

2015 ◽  
Vol 4 (2) ◽  
pp. 67-77
Author(s):  
Lior Turgeman ◽  
Tsviya Nayhoz ◽  
Nir Roth ◽  
Gilad Yahav ◽  
Avraham Hirshberg ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
System Optimization ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging

P‐103: Non‐Destructive FMM Shadow‐Level Evaluation with Fluorescence Lifetime Imaging Microscopy

2021 ◽  
Vol 52 (1) ◽  
pp. 1466-1469
Author(s):  
Ki Young Yeon ◽  
Woo Young Lee ◽  
Junghan Shin ◽  
Young Gil Park ◽  
Nari Ahn
Keyword(s):  
Fluorescence Lifetime ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging ◽  
Non Destructive

scholarly journals Direct organelle thermometry with fluorescence lifetime imaging microscopy in single myotubes

2016 ◽  
Vol 52 (24) ◽  
pp. 4458-4461 ◽  
Author(s):  
Hideki Itoh ◽  
Satoshi Arai ◽  
Thankiah Sudhaharan ◽  
Sung-Chan Lee ◽  
Young-Tae Chang ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Heat Production ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging ◽  
C2c12 Myotube

FLIM of ER thermo yellow and non-targeted mCherry reveals the Ca2+-dependent heat production localized to SR in C2C12 myotube.

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