scholarly journals A subcellular cookie cutter for spatial genomics in human tissue

2021 ◽  
Author(s):  
Alexander Bury ◽  
Angela Pyle ◽  
Fabio Marcuccio ◽  
Doug Turnbull ◽  
Amy Vincent ◽  
...  
Keyword(s):  
Laser Capture Microdissection ◽  
Human Tissue ◽  
The State ◽  
Precise Location ◽  
Cellular Physiology ◽  
First Time ◽  
Laser Capture ◽  
State Of Art ◽  
Biopsy Technology

Intracellular heterogeneity contributes significantly to cellular physiology and, in a number of debilitating diseases, cellular pathophysiology. This is greatly influenced by distinct organelle populations and to understand the aetiology of disease it is important to have tools able to isolate and differentially analyse organelles from precise location within tissues. Here we report the development of a subcellular biopsy technology that facilitates the isolation of organelles, such as mitochondria, from human tissue. We compared the subcellular biopsy technology to laser capture microdissection (LCM) that is the state of art technique for the isolation of cells from their surrounding tissues. We demonstrate an operational limit of (>20 micron) for LCM and then, for the first time in human tissue, show that subcellular biopsy can be used to isolate mitochondria beyond this limit.

scholarly journals A novel Microproteomic Approach Using Laser Capture Microdissection to Study Cellular Protrusions

2019 ◽  
Vol 20 (5) ◽  
pp. 1172 ◽  
Author(s):  
Karine Gousset ◽  
Ana Gordon ◽  
Shravan Kumar Kannan ◽  
Joey Tovar
Keyword(s):  
Laser Capture Microdissection ◽  
Protein Extraction ◽  
Cell Communication ◽  
Protein Composition ◽  
Fluorescent Markers ◽  
Different Types ◽  
Health And Disease ◽  
Multicellular Organisms ◽  
First Time ◽  
Laser Capture

Cell–cell communication is vital to multicellular organisms, and distinct types of cellular protrusions play critical roles during development, cell signaling, and the spreading of pathogens and cancer. The differences in the structure and protein composition of these different types of protrusions and their specific functions have not been elucidated due to the lack of a method for their specific isolation and analysis. In this paper, we described, for the first time, a method to specifically isolate distinct protrusion subtypes, based on their morphological structures or fluorescent markers, using laser capture microdissection (LCM). Combined with a unique fixation and protein extraction protocol, we pushed the limits of microproteomics and demonstrate that proteins from LCM-isolated protrusions can successfully and reproducibly be identified by mass spectrometry using ultra-high field Orbitrap technologies. Our method confirmed that different types of protrusions have distinct proteomes and it promises to advance the characterization and the understanding of these unique structures to shed light on their possible role in health and disease.

scholarly journals Combined laser capture microdissection and serial analysis of gene expression from human tissue samples

2004 ◽  
Vol 18 (4) ◽  
pp. 577-584 ◽  
Author(s):  
Jeong Hee Cho-Vega ◽  
Patricia Troncoso ◽  
Kim-Anh Do ◽  
Carlo Rago ◽  
Xuemei Wang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Laser Capture Microdissection ◽  
Human Tissue ◽  
Tissue Samples ◽  
Laser Capture

scholarly journals Searching for impossible subspace trails and improved impossible differential characteristics for SIMON-like block ciphers

Cybersecurity ◽  
2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Xuzi Wang ◽  
Baofeng Wu ◽  
Lin Hou ◽  
Dongdai Lin
Keyword(s):  
Great Increase ◽  
Search Space ◽  
Block Ciphers ◽  
The State ◽  
Input Output ◽  
Impossible Differential ◽  
Milp Model ◽  
First Time ◽  
Impossible Differentials ◽  
State Of Art

AbstractIn this paper, we greatly increase the number of impossible differentials for SIMON and SIMECK by eliminating the 1-bit constraint in input/output difference, which is the precondition to ameliorate the complexity of attacks. We propose an algorithm which can greatly reduce the searching complexity to find such trails efficiently since the search space exponentially expands to find impossible differentials with multiple active bits. There is another situation leading to the contradiction in impossible differentials except for miss-in-the-middle. We show how the contradiction happens and conclude the precondition of it defined as miss-from-the-middle. It makes our results more comprehensive by applying these two approach simultaneously. This paper gives for the first time impossible differential characteristics with multiple active bits for SIMON and SIMECK, leading to a great increase in the number. The results can be verified not only by covering the state-of-art, but also by the MILP model.

scholarly journals Mapping Genomic Scaffolds to Chromosomes Using Laser Capture Microdissection in Application to Hawaiian Picture-Winged Drosophila

2017 ◽  
Vol 152 (4) ◽  
pp. 204-212 ◽  
Author(s):  
Lin Kang ◽  
Phillip George ◽  
Donald K. Price ◽  
Igor Sharakhov ◽  
Pawel Michalak
Keyword(s):  
Laser Capture Microdissection ◽  
De Novo ◽  
Chromosome Mapping ◽  
Drosophila Species ◽  
Sequencing Technologies ◽  
Physical Maps ◽  
New Information ◽  
Genome Assemblies ◽  
First Time ◽  
Laser Capture

Next-generation sequencing technologies have led to a decreased cost and an increased throughput in genome sequencing. Yet, many genome assemblies based on short sequencing reads have been assembled only to the scaffold level due to the lack of sufficient chromosome mapping information. Traditional ways of mapping scaffolds to chromosomes require a large amount of laboratory work and time to generate genetic and/or physical maps. To address this problem, we conducted a rapid technique which uses laser capture microdissection and enables mapping scaffolds of de novo genome assemblies directly to chromosomes in Hawaiian picture-winged Drosophila. We isolated and sequenced intact chromosome arms from larvae of D. differens. By mapping the reads of each chromosome to the recently assembled scaffolds from 3 Hawaiian picture-winged Drosophila species, at least 67% of the scaffolds were successfully assigned to chromosome arms. Even though the scaffolds are not ordered within a chromosome, the fast-generated chromosome information allows for chromosome-related analyses after genome assembling. We utilize this new information to test the faster-X evolution effect for the first time in these Hawaiian picture-winged Drosophila species.

Isolating Human Notochord Using Laser Capture Microdissection

2016 ◽  
Vol 77 (S 02) ◽  
Author(s):  
Youssef Yakkioui ◽  
Remco Santegoeds ◽  
Koo van Overbeeke ◽  
Andreas Herrler ◽  
Yasin Temel
Keyword(s):  
Laser Capture Microdissection ◽  
Laser Capture

Politični položaj na Slovenskem leta 1919

2020 ◽  
Vol 20 (2020) (2) ◽  
pp. 359-394
Author(s):  
Jurij Perovšek
Keyword(s):  
State Government ◽  
The State ◽  
The Political ◽  
Liberal Party ◽  
New Era ◽  
Party Organisation ◽  
Western Border ◽  
Historical Phenomenon ◽  
First Time ◽  
Similar Development

For Slovenes in the Kingdom of the Serbs, Croats and Slovenes the year 1919 represented the final step to a new political beginning. With the end of the united all-Slovene liberal party organisation and the formation of separate liberal parties, the political party life faced a new era. Similar development was showing also in the Marxist camp. The Catholic camp was united. For the first time, Slovenes from all political camps took part in the state government politics and parliament work. They faced the diminishing of the independence, which was gained in the State of Slovenes, Croats and Serbs, and the mutual fight for its preservation or abolition. This was the beginning of national-political separations in the later Yugoslav state. The year 1919 was characterized also by the establishment of the Slovene university and early occurrences of social discontent. A declaration about the new historical phenomenon – Bolshevism, had to be made. While the region of Prekmurje was integrated to the new state, the questions of the Western border and the situation with Carinthia were not resolved. For the Slovene history, the year 1919 presents a multi-transitional year.

Islamic and Jewish Religious Feminists Tackle Islamic and Jewish Oral Law: Maintenance and Rebellion of Wives

2017 ◽  
Vol 11 (1) ◽  
pp. 35-63
Author(s):  
Ruth Roded
Keyword(s):  
State Policy ◽  
Political System ◽  
Daily Life ◽  
The State ◽  
The Political ◽  
Jewish Law ◽  
Modern Times ◽  
Parallel Case ◽  
First Time

Beginning in the early 1970s, Jewish and Muslim feminists, tackled “oral law”—Mishna and Talmud, in Judaism, and the parallel Hadith and Fiqh in Islam, and several analogous methodologies were devised. A parallel case study of maintenance and rebellion of wives —mezonoteha, moredet al ba?ala; nafaqa al-mar?a and nush?z—in classical Jewish and Islamic oral law demonstrates similarities in content and discourse. Differences between the two, however, were found in the application of oral law to daily life, as reflected in “responsa”—piskei halacha and fatwas. In modern times, as the state became more involved in regulating maintenance and disobedience, and Jewish law was backed for the first time in history by a state, state policy and implementation were influenced by the political system and socioeconomic circumstances of the country. Despite their similar origin in oral law, maintenance and rebellion have divergent relevance to modern Jews and Muslims.

Opponent Center-Surround Contrast: Colour to Grey Conversion

2020 ◽  
Vol 2020 (1) ◽  
pp. 105-108
Author(s):  
Ali Alsam
Keyword(s):  
Evolutionary Algorithms ◽  
Three Dimensional ◽  
The State ◽  
Colour Space ◽  
Vision Science ◽  
State Of Art ◽  
Over Time

Vision is the science that informs us about the biological and evolutionary algorithms that our eyes, opticnerves and brains have chosen over time to see. This article is an attempt to solve the problem of colour to grey conversion, by borrowing ideas from vision science. We introduce an algorithm that measures contrast along the opponent colour directions and use the results to combine a three dimensional colour space into a grey. The results indicate that the proposed algorithm competes with the state of art algorithms.

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