scholarly journals Leaf Spray Mass Spectrometry: A Rapid Ambient Ionization Technique to Directly Assess Metabolites from Plant Tissues

10.3791/57949 ◽  
2018 ◽  
Author(s):  
Dana M. Freund ◽  
Katherine A. Sammons ◽  
Nokwanda P. Makunga ◽  
Jerry D. Cohen ◽  
Adrian D. Hegeman
Keyword(s):  
Mass Spectrometry ◽  
Ambient Ionization ◽  
Plant Tissues ◽  
Ionization Technique

Thermal bursting ionization for ambient mass spectrometry

RSC Advances ◽  
2016 ◽  
Vol 6 (3) ◽  
pp. 2496-2499 ◽  
Author(s):  
Jiying Pei ◽  
Kefu Yu ◽  
Yinghui Wang
Keyword(s):  
Mass Spectrometry ◽  
Ambient Ionization ◽  
Ambient Mass Spectrometry ◽  
Sample Analysis ◽  
Liquid Sample ◽  
Ionization Source ◽  
Complex Liquid

Ambient ionization source, thermal bursting ionization (TBI), was characterized for complex liquid sample analysis with mass spectrometry.

scholarly journals Applications of ambient ionization mass spectrometry in 2020: An annual review

2021 ◽  
Vol 2 (3-4) ◽  
pp. 193-212
Author(s):  
Stephanie Rankin‐Turner ◽  
Liam M. Heaney
Keyword(s):  
Mass Spectrometry ◽  
Ambient Ionization ◽  
Ionization Mass Spectrometry ◽  
Annual Review ◽  
Ionization Mass ◽  
Ambient Ionization Mass Spectrometry

scholarly journals Mass Spectrometry Imaging of Bio‐oligomer Polydispersity in Plant Tissues by Laser Desorption Ionization from Silicon Nanopost Arrays

2021 ◽  
Vol 133 (16) ◽  
pp. 9153-9159
Author(s):  
Laith Z. Samarah ◽  
Tina H. Tran ◽  
Gary Stacey ◽  
Akos Vertes
Keyword(s):  
Mass Spectrometry ◽  
Mass Spectrometry Imaging ◽  
Laser Desorption ◽  
Plant Tissues ◽  
Laser Desorption Ionization ◽  
Desorption Ionization

scholarly journals 662. Identification of Clinically Relevant Microbes with the MasSpec Pen

2020 ◽  
Vol 7 (Supplement_1) ◽  
pp. S386-S387
Author(s):  
Sydney C Povilaitis ◽  
Ashish D Chakraborty ◽  
Rachel D Downey ◽  
Sarmistha Bhaduri Hauger ◽  
Livia Eberlin
Keyword(s):  
Mass Spectrometry ◽  
Mass Spectrometer ◽  
Model Performance ◽  
Sample Surface ◽  
Clinical Analysis ◽  
Ambient Ionization ◽  
Rapid Identification ◽  
Negative Ion ◽  
Ionization Mass ◽  
Molecular Features

Abstract Background In the age of antimicrobial resistance, rapid identification of infectious agents is critical for antimicrobial stewardship and effective therapy. To this end, ambient ionization mass spectrometry techniques have been applied for rapid identification of microbes directly from culture isolates. We have developed a handheld, mass spectrometry-based device, the MasSpec Pen, that permits direct molecular analysis of a biological sample in seconds (Scheme 1). Here, we employ the MasSpec Pen to identify clinically relevant microbes directly from culture isolates. Methods Staphylococcus aureus, Staphylococcus epidermidis, Group A and B Streptococcus, Kingella kingae (K.k), and Pseudomonas aeruginosa (P.a) were cultured on 5% sheep’s blood nutrient agar at 37 °C overnight. Colonies were transferred to a glass slide where they were analyzed directly with the MasSpec Pen coupled to a Q Exactive mass spectrometer (Thermo Scientific) in negative ion mode. For MasSpec Pen analysis, a 10 µL droplet of water was held in contact with the sample surface for 3 seconds and then aspirated to the mass spectrometer for analysis. Data was normalized and the molecular features resulting from the analysis solvent and nutrient medium were removed. The least absolute shrinkage and selection operator (lasso) statistical method was used to build classification models for prediction of bacterial identity. Model performance was evaluated by leave-one-out cross-validation and a validation set of samples. Scheme 1: MasSpec Pen workflow Results Various small molecules were detected including metabolites and glycerophospholipid species. The mass spectral profiles for each species exhibited qualitative differences among them (Figure 1). Additionally, several quorum-sensing molecules were observed in P.a. including hydroxy-heptyl-quinoline (m/z 242.155). Lasso statistical classifiers were created to differentiate organisms at the level of Gram type, genus, and species with each model comprised of a sparse set of molecular features. Accuracies of 90% or greater were achieved for all lasso models and as high as 98% for the differentiation of Staphylococcus (Staph.) and Streptococcus (Strep.). Figure 1: Molecular profiles of species analyzed Figure 2: Statistical classification results Conclusion These results demonstrate the potential of the MasSpec Pen as a tool for clinical analysis of infected biospecimens. Disclosures Sydney C. Povilaitis, BA, MS Pen Technologies, Inc. (Other Financial or Material Support, Patent) Livia Eberlin, PhD, MS Pen Technolpogies, Inc. (Board Member, Shareholder)

Ambient Electric Arc Ionization for Versatile Sample Analysis Using Mass Spectrometry

The Analyst ◽  
2021 ◽  
Author(s):  
Yuanji Gao ◽  
Yuan Li ◽  
Binpeng Zhan ◽  
Quan He ◽  
Heping Zhu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Electric Arc ◽  
Ion Source ◽  
Sample Analysis ◽  
Ionization Technique ◽  
Soft Ionization

A novel, convenient ambient electric arc ionization (AEAI) device was developed as mass spectrometry ion source for versatile sample analysis. AEAI could be considered as a soft ionization technique in...

Front Cover: Mass spectrometry imaging for spatial visualization of organic components in plant tissues

2021 ◽  
Vol 44 (18) ◽  
Author(s):  
Wenya Hu ◽  
Yehua Han ◽  
Yiqi Sheng ◽  
Yinghao Wang ◽  
Qiong Pan ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Mass Spectrometry Imaging ◽  
Spatial Visualization ◽  
Plant Tissues ◽  
Front Cover ◽  
Organic Components

scholarly journals A cautionary note on the effects of laboratory air contaminants on ambient ionization mass spectrometry measurements

2017 ◽  
Vol 31 (19) ◽  
pp. 1659-1668 ◽  
Author(s):  
Sambhav R. Kumbhani ◽  
Lisa M. Wingen ◽  
Véronique Perraud ◽  
Barbara J. Finlayson-Pitts
Keyword(s):  
Mass Spectrometry ◽  
Ambient Ionization ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
Cautionary Note ◽  
Air Contaminants ◽  
Ambient Ionization Mass Spectrometry
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