Determination ofo,oEDDHA - a xenobiotic chelating agent used in Fe fertilizers - in plant tissues by liquid chromatography/electrospray mass spectrometry: overcoming matrix effects

2009 ◽  
Vol 23 (11) ◽  
pp. 1694-1702 ◽  
Author(s):  
Irene Orera ◽  
Anunciación Abadía ◽  
Javier Abadía ◽  
Ana Álvarez-Fernández
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Electrospray Mass Spectrometry ◽  
Matrix Effects ◽  
Chelating Agent ◽  
Plant Tissues

scholarly journals Analytical Methods for Quantification and Identification of Intact Glucosinolates in Arabidopsis Roots Using LC-QqQ(LIT)-MS/MS

Metabolites ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 47
Author(s):  
Kourosh Hooshmand ◽  
Inge S. Fomsgaard
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Crude Extract ◽  
Matrix Effects ◽  
Critical Role ◽  
Reversed Phase ◽  
Biologically Active ◽  
Natural Soil ◽  
Chromatography Method ◽  
Arabidopsis Root

Glucosinolates are biologically active secondary metabolites in Brassicaceae plants that play a critical role in positive and negative interactions between plants and root-associated microbial communities. The aim of this study was to develop a reversed-phase liquid chromatography method to quantify and identify intact glucosinolates in the root of Arabidopsis thaliana (Arabidopsis) grown in non-sterile natural soil by using liquid chromatography-hybrid triple quadruple-linear ion trap (LC-QqQ(LIT)) mass spectrometry. The Synergi Fusion C18-based column was found to be effective for sufficient retention and separation of nine intact glucosinolates without the need for time-consuming desulfation or ion-pairing steps. Method validation results showed satisfactory inter-day and intra-day precision for all glucosinolates except for 4-hydroxyglucobrassicin. Good inter-day and intra-day accuracy and recovery results were observed for glucoiberin, gluconapin, glucobrassicin, 4-methoxyglucobrassicin and neoglucobrassicin. However, for 4-hydroxyglucobrassicin, glucoraphanin and glucoerucin corrections to quantification results might be necessary since the recovery and accuracy results were not optimal. Matrix effects were shown to have a negligible effect on the ionization of all target analytes. The established liquid chromatography–tandem mass spectrometry (LC-MS/MS) method was applied to quantify target intact glucosinolates in Arabidopsis root crude extract of four different wild-type accessions where differences in terms of concentration and composition of intact glucosinolates were observed. Employment of sensitive and selective precursor ion survey scan of m/z 97 in combination with the information-dependent acquisition (IDA) of the enhanced product ion (EPI) dependent scan (Prec97-IDA-EPI) using LC-QqQ(LIT) provided high confidence in structural characterization of diverse intact glucosinolate profiles in complex Arabidopsis root crude extract.

scholarly journals Determination of synthetic ferric chelates used as fertilizers by liquid chromatography-electrospray/mass spectrometry in agricultural matrices

2007 ◽  
Vol 18 (1) ◽  
pp. 37-47 ◽  
Author(s):  
Ana Álvarez-Fernández ◽  
Irene Orera ◽  
Javier Abadía ◽  
Anunciación Abadía
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Electrospray Mass Spectrometry

Development of a Nanoscale Liquid Chromatography/Electrospray Mass Spectrometry Methodology for the Detection and Identification of DNA Adducts

1997 ◽  
Vol 69 (16) ◽  
pp. 3161-3168 ◽  
Author(s):  
K. Vanhoutte ◽  
W. Van Dongen ◽  
I. Hoes ◽  
F. Lemière ◽  
E. L. Esmans ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Dna Adducts ◽  
Electrospray Mass Spectrometry ◽  
Detection And Identification

Liquid chromatography–electrospray mass spectrometry multi-residue determination of pesticides in apples and pears

1999 ◽  
Vol 830 (1) ◽  
pp. 135-143 ◽  
Author(s):  
E Lacassie ◽  
M.-F Dreyfuss ◽  
J.L Daguet ◽  
M Vignaud ◽  
P Marquet ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Electrospray Mass Spectrometry ◽  
Residue Determination

Overcoming Matrix Effects in Liquid Chromatography−Mass Spectrometry

2008 ◽  
Vol 80 (23) ◽  
pp. 9343-9348 ◽  
Author(s):  
Achille Cappiello ◽  
Giorgio Famiglini ◽  
Pierangela Palma ◽  
Elisabetta Pierini ◽  
Veronica Termopoli ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Matrix Effects ◽  
Liquid Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry
Sign in / Sign up

Export Citation Format

Share Document