Imaging of Extracellular Vesicles by Atomic Force Microscopy

10.3791/59254 ◽  
2019 ◽  
Author(s):  
Mikhail Skliar ◽  
Vasiliy S. Chernyshev
Keyword(s):  
Atomic Force Microscopy ◽  
Extracellular Vesicles ◽  
Force Microscopy ◽  
Atomic Force

Study of NSCLC cell migration promoted by NSCLC-Derived Extracellular Vesicle using atomic force microscopy

2021 ◽  
Author(s):  
Shuwei Wang ◽  
Jiajia Wang ◽  
Tuoyu Ju ◽  
Kaige Qu ◽  
Fan Yang ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Cell Migration ◽  
Cancer Cells ◽  
Extracellular Vesicles ◽  
Extracellular Vesicle ◽  
Cancer Microenvironment ◽  
Biological Processes ◽  
Force Microscopy ◽  
Atomic Force ◽  
Cellular Components

Extracellular Vesicles (EVs) secreted by cancer cells have a key role in the cancer microenvironment and progression. Previous studies have mainly focused on molecular functions, cellular components and biological processes...

High-fidelity probing of the structure and heterogeneity of extracellular vesicles by resonance-enhanced atomic force microscopy infrared spectroscopy

2019 ◽  
Vol 14 (2) ◽  
pp. 576-593 ◽  
Author(s):  
Sally Yunsun Kim ◽  
Dipesh Khanal ◽  
Bill Kalionis ◽  
Wojciech Chrzanowski
Keyword(s):  
Atomic Force Microscopy ◽  
Infrared Spectroscopy ◽  
Extracellular Vesicles ◽  
High Fidelity ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals Perspectives of Microscopy Methods for Morphology Characterisation of Extracellular Vesicles from Human Biofluids

Biomedicines ◽  
2021 ◽  
Vol 9 (6) ◽  
pp. 603
Author(s):  
Mladenka Malenica ◽  
Marija Vukomanović ◽  
Mario Kurtjak ◽  
Valentina Masciotti ◽  
Simone dal Zilio ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Atomic Force Microscopy ◽  
Cerebrospinal Fluid ◽  
Sample Preparation ◽  
Extracellular Vesicles ◽  
Saline Solution ◽  
Force Microscopy ◽  
Atomic Force ◽  
Current State ◽  
Potential Benefits

Extracellular vesicles (EVs) are nanometric membranous structures secreted from almost every cell and present in biofluids. Because EV composition reflects the state of its parental tissue, EVs possess an enormous diagnostic/prognostic potential to reveal pathophysiological conditions. However, a prerequisite for such usage of EVs is their detailed characterisation, including visualisation which is mainly achieved by atomic force microscopy (AFM) and electron microscopy (EM). Here we summarise the EV preparation protocols for AFM and EM bringing out the main challenges in the imaging of EVs, both in their natural environment as biofluid constituents and in a saline solution after EV isolation. In addition, we discuss approaches for EV imaging and identify the potential benefits and disadvantages when different AFM and EM methods are applied, including numerous factors that influence the morphological characterisation, standardisation, or formation of artefacts. We also demonstrate the effects of some of these factors by using cerebrospinal fluid as an example of human biofluid with a simpler composition. Here presented comparison of approaches to EV imaging should help to estimate the current state in morphology research of EVs from human biofluids and to identify the most efficient pathways towards the standardisation of sample preparation and microscopy modes.

scholarly journals Biomechanical Properties of Blood Plasma Extracellular Vesicles Revealed by Atomic Force Microscopy

Biology ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 4
Author(s):  
Viktor Bairamukov ◽  
Anton Bukatin ◽  
Sergey Landa ◽  
Vladimir Burdakov ◽  
Tatiana Shtam ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Blood Plasma ◽  
Extracellular Vesicles ◽  
Water Film ◽  
Biomechanical Properties ◽  
Internal Cavity ◽  
Practical Applications ◽  
Force Microscopy ◽  
Atomic Force ◽  
The Difference

While extracellular vesicles (EVs) are extensively studied by various practical applications in biomedicine, there is still little information on their biomechanical properties due to their nanoscale size. We identified isolated blood plasma vesicles that carried on biomarkers associated with exosomes and exomeres and applied atomic force microscopy (AFM) to study them at single particle level in air and in liquid. Air measurements of exosomes revealed a mechanically indented internal cavity in which highly adhesive sites were located. In contrast, the highly adhesive sites of exomeres were located at the periphery and the observed diameter of the particles was ~35 nm. In liquid, the reversible deformation of the internal cavity of exosomes was observed and a slightly deformed lipid bi-layer was identified. In contrast, exomeres were not deformed and their observed diameter was ~16 nm. The difference in diameters might be associated with a higher sorption of water film in air. The parameters we revealed correlated with the well-known structure and function for exosomes and were observed for exomeres for the first time. Our data provide a new insight into the biomechanical properties of nanoparticles and positioned AFM as an exclusive source of in situ information about their biophysical characteristics.

Atomic force microscopy analysis of extracellular vesicles

2017 ◽  
Vol 46 (8) ◽  
pp. 813-820 ◽  
Author(s):  
P. Parisse ◽  
I. Rago ◽  
L. Ulloa Severino ◽  
F. Perissinotto ◽  
E. Ambrosetti ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Extracellular Vesicles ◽  
Atomic Force Microscopy Analysis ◽  
Force Microscopy ◽  
Atomic Force ◽  
Microscopy Analysis

Dimensional characterization of extracellular vesicles using atomic force microscopy

2017 ◽  
Vol 28 (3) ◽  
pp. 034006 ◽  
Author(s):  
N Sebaihi ◽  
B De Boeck ◽  
Y Yuana ◽  
R Nieuwland ◽  
J Pétry
Keyword(s):  
Atomic Force Microscopy ◽  
Extracellular Vesicles ◽  
Force Microscopy ◽  
Atomic Force

Ascent of atomic force microscopy as a nanoanalytical tool for exosomes and other extracellular vesicles

2018 ◽  
Vol 29 (13) ◽  
pp. 132001 ◽  
Author(s):  
S Sharma ◽  
M LeClaire ◽  
J K Gimzewski
Keyword(s):  
Atomic Force Microscopy ◽  
Extracellular Vesicles ◽  
Force Microscopy ◽  
Atomic Force
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