scholarly journals Live-Cell Forward Genetic Approach to Identify and Isolate Developmental Mutants in Chlamydia trachomatis

10.3791/61365 ◽  
2020 ◽  
Author(s):  
Travis J. Chiarelli ◽  
Nicole A. Grieshaber ◽  
Scott S. Grieshaber
Keyword(s):  
Chlamydia Trachomatis ◽  
Live Cell ◽  
Genetic Approach ◽  
Developmental Mutants

scholarly journals Red Fluorescent Chlamydia trachomatis Applied to Live Cell Imaging and Screening for Antibacterial Agents

2018 ◽  
Vol 9 ◽  
Author(s):  
Sergio A. Mojica ◽  
Anna U. Eriksson ◽  
Rohan A. Davis ◽  
Wael Bahnan ◽  
Mikael Elofsson ◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
Live Cell Imaging ◽  
Antibacterial Agents ◽  
Cell Imaging ◽  
Live Cell

scholarly journals Single-Inclusion Kinetics of Chlamydia trachomatis Development

mSystems ◽  
2020 ◽  
Vol 5 (5) ◽  
Author(s):  
Travis J. Chiarelli ◽  
Nicole A. Grieshaber ◽  
Anders Omsland ◽  
Christopher H. Remien ◽  
Scott S. Grieshaber
Keyword(s):  
Chlamydia Trachomatis ◽  
Mathematical Models ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Host Cells ◽  
Developmental Cycle ◽  
Cell Type ◽  
Content Type ◽  
Obligate Intracellular

ABSTRACT The obligate intracellular bacterial pathogen Chlamydia trachomatis is reliant on a developmental cycle consisting of two cell forms, termed the elementary body (EB) and the reticulate body (RB). The EB is infectious and utilizes a type III secretion system and preformed effector proteins during invasion, but it does not replicate. The RB replicates in the host cell but is noninfectious. This developmental cycle is central to chlamydial pathogenesis. In this study, we developed mathematical models of the developmental cycle that account for potential factors influencing RB-to-EB cell type switching during infection. Our models predicted that two categories of regulatory signals for RB-to-EB development could be differentiated experimentally, an “intrinsic” cell-autonomous program inherent to each RB and an “extrinsic” environmental signal to which RBs respond. To experimentally differentiate between mechanisms, we tracked the expression of C. trachomatis development-specific promoters in individual inclusions using fluorescent reporters and live-cell imaging. These experiments indicated that EB production was not influenced by increased multiplicity of infection or by superinfection, suggesting the cycle follows an intrinsic program that is not directly controlled by environmental factors. Additionally, live-cell imaging revealed that EB development is a multistep process linked to RB growth rate and cell division. The formation of EBs followed a progression with expression from the euo and ihtA promoters evident in RBs, while expression from the promoter for hctA was apparent in early EBs/IBs. Finally, expression from the promoters for the true late genes, hctB, scc2, and tarp, was evident in the maturing EB. IMPORTANCE Chlamydia trachomatis is an obligate intracellular bacterium that can cause trachoma, cervicitis, urethritis, salpingitis, and pelvic inflammatory disease. To establish infection in host cells, Chlamydia must complete a multiple-cell-type developmental cycle. The developmental cycle consists of specialized cells, the EB cell, which mediates infection of new host cells, and the RB cell, which replicates and eventually produces more EB cells to mediate the next round of infection. By developing and testing mathematical models to discriminate between two competing hypotheses for the nature of the signal controlling RB-to-EB cell type switching, we demonstrate that RB-to-EB development follows a cell-autonomous program that does not respond to environmental cues. Additionally, we show that RB-to-EB development is a function of chlamydial growth and division. This study serves to further our understanding of the chlamydial developmental cycle that is central to the bacterium’s pathogenesis.

Screening auf Chlamydia trachomatis Infektion in der Schwangerschaft

2008 ◽  
Vol 68 (S 01) ◽  
Author(s):  
TM Weissenbacher ◽  
A Gingelmaier ◽  
M Kupka ◽  
F Kainer ◽  
K Friese ◽  
...  
Keyword(s):  
Chlamydia Trachomatis

Udział atypowego patogenu Chlamydia trachomatis w zapaleniach cewki moczowej

2016 ◽  
Vol 7 (6) ◽  
pp. 425-428
Author(s):  
Magdalena Frej-Mądrzak ◽  
Jolanta Sarowska ◽  
Agnieszka Jama-Kmiecik ◽  
Dorota Teryks-Wołyniec ◽  
Irena Choroszy-Król
Keyword(s):  
Chlamydia Trachomatis

Genetic Approach To The School Didactics Analysis: The Experience Of Applications

2018 ◽  
Author(s):  
Olga N. Machekhina
Keyword(s):  
Genetic Approach

MYCOPLASMA AND CHLAMYDIA AS ETHIOLOGICAL FACTORS OF BRONCHIAL ASTHMA IN TERMS OF ETHNOGENESIS

2013 ◽  
Vol 68 (7) ◽  
pp. 57-60
Author(s):  
O. A. Sharavii ◽  
S. V. Smirnova
Keyword(s):  
Immune Response ◽  
Chlamydia Trachomatis ◽  
Blood Serum ◽  
Bronchial Asthma ◽  
Mycoplasma Hominis ◽  
Acute Stage ◽  
Control Group ◽  
Clinical Markers ◽  
Chlamydophila Psittaci ◽  
Asthma Patients

 Aim. The study of the prevalence and clinical peculiarities of Mycoplasmosis and Chlamydiosis in patients with different pathogenic forms of bronchial asthma (BA) taking into account ethnicity of a patient. Subjects and Methods. The research covered 239 subjects – both the Europeoids and the Mongoloids in the city of Krasnoyarsk and the town of Kyzyl, all of them being BA patients of different stages, including acute stage and practically healthy. We had determined antigens Mycoplasma pneumoniae, Mycoplasma hominis, Chlamydophila pneumoniae, Chlamydophila psittaci and Chlamydia trachomatis in smears of mucosa of pharynx and antibodies to these antigens in peripheral blood serum. Results.  We found high frequency of Mycoplasmosis and Chlamydiosis in the inhabitants of Eastern Siberia, BA patients with different pathogenic forms as compared to control group. We had determined ethnic peculiarities of specific immune response: IgM to М. pneumoniae was revealed in the Europoids more frequently than in the Mongoloids, but IgM to С. pneumoniae and to C. trachomatis, C. trachomatis antigens had been revealed more often in the Mongoloids than in the Europoids. We accepted as clinical equivalents of Mycoplasmosis and Chlamydiosis diagnostics the following signs: temperature around 37C (subfebrile temperature), non-intensive but stable coughing with scanty mucous and muco-purulent sputum, dyspnea of mixed character. Conclusions. Mycoplasma and Chlamydia are meaningful etiologic factors of bronchial asthma. We have found the peculiarities of immune response depending on ethnicity of a patient (ethnic belonging). Clinical markers of Mycoplasmosis and Chlamydiosis should be taken into account in bronchial asthma in order to provide diagnostics timely as well as eradication of infection agents. Because of insufficient knowledge of problem of bronchial asthma related to contamination with Мycoplasma and Chlamydia we put the goal to study the frequency of Mycoplasmosis and Chlamydiosis occurrence in bronchial asthma patients and determine the characteristics clinical course of diseases. We defined antigens Мycoplasma pneumoniae, Мycoplasma hominis, Chlamydophila pneumoniaе, Chlamydophila psittaci, Chlamydia trachomatis in smears of oropharynx mucosa and antibodies to them in blood serum. 

Exemplar Abstract for Chlamydia trachomatis (Busacca 1935) Rake 1957 (Approved Lists 1980) emend. García-López et al. 2019.

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Dorothea Taylor ◽  
George M Garrity
Keyword(s):  
Chlamydia Trachomatis
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