MYCOPLASMA AND CHLAMYDIA AS ETHIOLOGICAL FACTORS OF BRONCHIAL ASTHMA IN TERMS OF ETHNOGENESIS

2013 ◽  
Vol 68 (7) ◽  
pp. 57-60
Author(s):  
O. A. Sharavii ◽  
S. V. Smirnova
Keyword(s):  
Immune Response ◽  
Chlamydia Trachomatis ◽  
Blood Serum ◽  
Bronchial Asthma ◽  
Mycoplasma Hominis ◽  
Acute Stage ◽  
Control Group ◽  
Clinical Markers ◽  
Chlamydophila Psittaci ◽  
Asthma Patients

 Aim. The study of the prevalence and clinical peculiarities of Mycoplasmosis and Chlamydiosis in patients with different pathogenic forms of bronchial asthma (BA) taking into account ethnicity of a patient. Subjects and Methods. The research covered 239 subjects – both the Europeoids and the Mongoloids in the city of Krasnoyarsk and the town of Kyzyl, all of them being BA patients of different stages, including acute stage and practically healthy. We had determined antigens Mycoplasma pneumoniae, Mycoplasma hominis, Chlamydophila pneumoniae, Chlamydophila psittaci and Chlamydia trachomatis in smears of mucosa of pharynx and antibodies to these antigens in peripheral blood serum. Results.  We found high frequency of Mycoplasmosis and Chlamydiosis in the inhabitants of Eastern Siberia, BA patients with different pathogenic forms as compared to control group. We had determined ethnic peculiarities of specific immune response: IgM to М. pneumoniae was revealed in the Europoids more frequently than in the Mongoloids, but IgM to С. pneumoniae and to C. trachomatis, C. trachomatis antigens had been revealed more often in the Mongoloids than in the Europoids. We accepted as clinical equivalents of Mycoplasmosis and Chlamydiosis diagnostics the following signs: temperature around 37C (subfebrile temperature), non-intensive but stable coughing with scanty mucous and muco-purulent sputum, dyspnea of mixed character. Conclusions. Mycoplasma and Chlamydia are meaningful etiologic factors of bronchial asthma. We have found the peculiarities of immune response depending on ethnicity of a patient (ethnic belonging). Clinical markers of Mycoplasmosis and Chlamydiosis should be taken into account in bronchial asthma in order to provide diagnostics timely as well as eradication of infection agents. Because of insufficient knowledge of problem of bronchial asthma related to contamination with Мycoplasma and Chlamydia we put the goal to study the frequency of Mycoplasmosis and Chlamydiosis occurrence in bronchial asthma patients and determine the characteristics clinical course of diseases. We defined antigens Мycoplasma pneumoniae, Мycoplasma hominis, Chlamydophila pneumoniaе, Chlamydophila psittaci, Chlamydia trachomatis in smears of oropharynx mucosa and antibodies to them in blood serum. 

scholarly journals The Role of Plant-Based Protein Functional Food in Preventing Acute Respiratory Disease: A Case Study

Nutrients ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 2116
Author(s):  
Andrei V. Tarasov ◽  
Rofail S. Rakhmanov ◽  
Elena S. Bogomolova ◽  
Ludmila A. Perminova ◽  
Zhanna L. Malakhova
Keyword(s):  
Immune Response ◽  
Treatment Group ◽  
Blood Cell ◽  
University Students ◽  
Blood Serum ◽  
Humoral Immunity ◽  
Functional Food ◽  
Respiratory Diseases ◽  
Protein Product ◽  
Control Group

The Kaliningrad region is known for its specific climate, which can negatively affect the adaptive potential of the body. This manifests in an increased incidence of respiratory diseases and skin conditions. To prevent high morbidity, a plant protein product was included in the diet of first-year university students. This study aimed to assess the effectiveness of this food intervention in preventing the most common diseases among Kaliningrad students. Two groups of university students took part in the food trial. In the control group, catabolic processes prevailed in nutrient metabolism. Disadaptation manifested itself in the metabolism of proteins, vitamins, minerals, hematopoiesis and humoral immunity. Inflammation was indicated by α1- and α2-globulins, a weak immune response, and IgM and IgG. High oxidative stress and low antioxidative ability of blood serum were observed. The plant-based protein product (FP) helped preserve testosterone level and prevent an increase in catabolic reactions. Moreover, it had a positive effect on both red blood cell hematopoiesis (a smaller increase in the average volume of erythrocytes, the same average concentration and content of hemoglobin, an increased relative red cell distribution width (RDW) and white blood cell hematopoiesis (a beneficial effect for the immune system: lymphocytes, the relative content of neutrophils, monocytes, basophils and eosinophils). The stimulation of humoral immunity was evidenced by beta- and gamma-globulins, an active immune response, the level of IgM and IgG, antioxidant protection, reduction of peroxides and an increase in antioxidant activity of blood serum. The 34-week observation showed a 1.7-fold decrease in the incidence of respiratory illnesses and a 5.7-fold decrease in skin and subcutaneous tissue diseases. Acute respiratory infections were reduced 1.8-fold. There were no cases of community-acquired pneumonia in the treatment group, compared with 55.1‰ in the control group. The incidence of respiratory diseases was 3.3–10.6 times lower in the treatment group than in the control group in weeks 6–19. The findings testify to the prophylactic effect of functional food during social adaptation and acclimatization of students.

scholarly journals Opistorchis felineus invasion influence on immunity in bronchial asthma

2010 ◽  
Vol 9 (3) ◽  
pp. 85-90
Author(s):  
L. M. Ogorodova ◽  
M. B. Freidin ◽  
A. E. Sazonov ◽  
O. S. Fyodorova ◽  
I. A. Deyev ◽  
...  
Keyword(s):  
Experimental Study ◽  
Immune Response ◽  
Bronchial Asthma ◽  
Cell Cultures ◽  
Molecular Mechanisms ◽  
Opisthorchis Felineus ◽  
Genes Expression ◽  
Asthma Patients ◽  
Human Immune Response ◽  
Human Immune

To investigate the molecular mechanisms of human immune response modification by Opisthorchis felineus antigens in bronchial asthma. The experimental study was performed with cell cultures from patients with bronchial asthma, patients with opisthorchiasis, and patients with BA and opisthorchiasis co-occurred. A proposed down-regulation of immune response by higher level of IL10 and TGFB genes expression in patients with opisthorchiasis was revealed.

scholarly journals Control tactics and evaluation of clinical efficacy of therapy in children with bronchial asthma associated with mycoplasma infection

2021 ◽  
Vol 99 (5) ◽  
pp. 35-41
Author(s):  
L. G. Gorina ◽  
N. А. Krylova ◽  
I. V. Rakovskaya ◽  
S А. Goncharova ◽  
O. I. Barkhatova
Keyword(s):  
Blood Serum ◽  
Bronchial Asthma ◽  
Asthma Exacerbation ◽  
Ureaplasma Urealyticum ◽  
Mycoplasma Hominis ◽  
Preschool Age ◽  
Mycoplasma Infection ◽  
Serum Samples ◽  
Mycoplasma Fermentans ◽  
Diagnostic Kits

The uncontrolled course of bronchial asthma (BA) in children and insufficient efficacy of standard therapy regimens may be due to underestimated infectious factors. The objective: to study specific parameters of the course and treatment of mycoplasma infection, improve monitoring over BA therapy in children of the tender and preschool age. Subjects and methods. 320 children with BA in the age from 1 to 7 years old were followed up. In this work, Mycoplasma pneumoniae (FH), Mycoplasma hominis (H-34), Ureaplasma urealyticum (serotype 8), Mycoplasma fermentans (PG18) and Mycoplasma arthritidis (PG6) were used, they were cultured on a liquid medium for cultivation of mycoplasmas and ureaplasmas. To isolate CIC from blood serum samples, we used the method of precipitation with 3.5% polyethylene glycol (PEG, 6000 Da), hemagglutination assays and IFA were used to identify mycoplasma antigens, mycoplasma DNA was detected by PCR with InterLabService diagnostic kits. The data of 47 patients with prolonged mycoplasma antigenemia were assessed at the baseline and in 1.5-3 months after the treatment course of azithromycin.Results. 320 blood serum samples from children with BA were tested, and the detection rate by hemagglutination assays of M. pneumoniae antigens was 60.9%, M. hominis – 43.4%, U. urealyticum – 44.8%, M. arthritidis – 29.7%, M. fermentrans – 45.3%. The assessment of relationship between of M. pneumoniae, M. hominis and asthma exacerbation showed that antigens of M. pneumoniae and M. hominis were found in 216 children (single or associated). After treatment with azithromycin, the frequency of BA exacerbations within 3 months decreased by 2.4 times, as well as there was a reduction in the number of samples positive for antigens and DNA of mycoplasma in a free state and within CIC. The persistence of antigens, DNA of M. pneumoniae and M. hominis before treatment of 47 children was 80.9 and 66.0% of cases, after treatment with azithromycin – 31.9 and 25.5% of cases, respectively (p < 0.001). Within CIC isolated from the blood serum of patients, antigens to M. pneumoniae and M. hominis before treatment were detected by IFA in 63.8 and 70.2% of children, after treatment – in 31.9 and 23.4%, respectively. p < 0.001. In blood samples, DNA of M. pneumoniae and M. hominis was detected by PCR before treatment in 8.5 and 34.0%; after treatment in 6.4% (p = 0.318) and 19.1% of cases, respectively (p = 0.009), and within CIC isolated from blood serum, in 27.7 and 48.9% of cases before treatment and 8.5 and 34.0% after it, respectively (p = 0.009).

scholarly journals FEATURES OF CYTOKINE PROFILE IN PATIENTS WITH BRONCHIAL ASTHMA COMBINED WITH OBESITY

2018 ◽  
Vol 20 (6) ◽  
pp. 913-920
Author(s):  
M. V. Antonyuk ◽  
T. A. Gvozdenko ◽  
T. P. Novgorodtseva ◽  
T. I. Vitkina ◽  
B. I. Geltser ◽  
...  
Keyword(s):  
Body Weight ◽  
Bronchial Asthma ◽  
Cytokine Profile ◽  
Control Group ◽  
Observation Group ◽  
Normal Body ◽  
Asthma Patients ◽  
Normal Body Weight ◽  
Anti Inflammatory ◽  
Group 1

Combination of bronchial asthma (BA) and obesity is a difficult-to-control phenotype. Studies of inflammatory process with respect to severity of the disease are important for understanding the potential influence of obesity on the BA clinical course. The objective of this study was to determine cytokine profile in patients with mild BA combined with obesity. The study involved fifty-three patients with partially controlled mild BA. The patients were recruited as volunteers and signed an informed consent. The first observation group consisted of 27 asthma patients with normal body weight, the second observation group consisted of 26 patients with BA combined with obesity. A control group included 25 healthy volunteers. All the patients underwent clinical and laboratory examination in accordance with clinical standards for BA and obesity. The levels of TNFα, IL-2, IL-4, IL-6, IL-10 were evaluated in blood serum by means of flow cytometry. The ratios of proand anti-inflammatory cytokines (TNFα/IL-4, TNFα/IL-10, IL-6/IL-4, IL-6/IL-10) were calculated. Asthma patients with obesity (the 2nd group) had elevated levels of IL-2 over control group and group 1, by 38% and 44% respectively(p < 0.05). The concentration of proinflammatory cytokines TNFα and IL-6 was significanty increased in the both patient groups. Mean TNFα level was increased 2.5 times (p < 0.05), and IL-6 levels were increased by 30% (p < 0.05) in the 1st group as compared to the controls. TNFα and IL-6 concentrations showed a 3-fold increase over control values (p < 0.05) in the 2nd group. The level of antiinflammatory cytokine IL-4 was increased in patients with BA, independently of body mass. It should be noted that the concentration of this cytokine in obese patients was higher by 29% than in patients with normal body weight. IL-10 levels in patients from the 2nd group were reduced more than 2 times than in the 1st group. The patients of the 1st group showed a decrease in the IL-6/IL-10 index, in comparison with control parameters, thus indicative of an imbalance due to the elevation of the anti-inflammatory IL-10 cytokine. Among BA patients with obesity (group 2) the TNFα/IL-10 and IL-6/IL-10 indexes were higher than those of the control group (2.3- and 5.5-fold, respectively) and the group 1 (2.6- and 2.5-fold, respectively). Dynamics of these indexes confirms the systemic nature of inflammation and a predominance of non-atopic  inflammation in asthma patients with obesity. Thus, features of the cytokine profile in BA with obesity consist of a significant increase in pro-inflammatory IL-2, IL-6, TNFα cytokines, and a relative decrease in anti-inflammatory IL- 10 cytokine. The development of BA with obesity, even in mild-severity BA, is accompanied by development of a cytokine disbalance, which is typical for a mixed-type inflammation, with a prevalence of neutrophil inflammation. 

scholarly journals Vaccination with the Chlamydia trachomatis Major Outer Membrane Protein Can Elicit an Immune Response as Protective as That Resulting from Inoculation with Live Bacteria

2005 ◽  
Vol 73 (12) ◽  
pp. 8153-8160 ◽  
Author(s):  
Sukumar Pal ◽  
Ellena M. Peterson ◽  
Luis M. de la Maza
Keyword(s):  
Immune Response ◽  
Chlamydia Trachomatis ◽  
Membrane Protein ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Major Outer Membrane Protein ◽  
Control Group

ABSTRACT BALB/c mice were vaccinated by the intramuscular (i.m.) and subcutaneous (s.c.) routes with a native preparation of the Chlamydia trachomatis mouse pneumonitis (MoPn) major outer membrane protein (MOMP), using Montanide ISA 720 and CpG-1826 as adjuvants. A negative control group was immunized with ovalbumin and the two adjuvants, and a positive control group was immunized intranasally (i.n.) with 104 inclusion-forming units (IFU) of C. trachomatis. Four weeks after the last i.m.-plus-s.c. immunization, mice were challenged in the ovarian bursa with 105 IFU of C. trachomatis MoPn. Six weeks after the genital challenge, animals were mated, and the pregnancies were monitored. After vaccination with MOMP, the mice developed strong Chlamydia-specific humoral and cellular immune responses. Following the genital challenge, of the mice vaccinated with the MOMP, only 15% (3/20) had positive vaginal cultures, while 85% (17/20) of the animals immunized with ovalbumin had positive cultures over the 6 weeks of observation (P < 0.05). Also, only 14% (3/21) of the animals inoculated i.n. with Chlamydia had positive vaginal cultures. After mating, 75% (15/20) of the mice vaccinated with MOMP carried embryos in both uterine horns. Of the animals vaccinated i.n. with the Chlamydia, 81% (17/21) had embryos in both uterine horns (P > 0.05). In contrast, only 10% (2/20) of the mice immunized with ovalbumin had embryos in both uterine horns (P < 0.05). In conclusion, immunization with a purified preparation of the MOMP is as effective as vaccination with viable C. trachomatis in eliciting a protective immune response against a genital challenge in mice.

scholarly journals Management of Psycho-Physiological aspect of Bronchial Asthma through Yogic Intervention

2019 ◽  
Vol 3 ◽  
pp. 42-48
Author(s):  
SARITA PRAJAPATI ◽  
RAKESH VARMA ◽  
AMAL K. DUTTA
Keyword(s):  
Bronchial Asthma ◽  
Stress Level ◽  
Forced Expiratory Volume ◽  
Control Group ◽  
Yoga Therapy ◽  
Therapy Program ◽  
Increasing Trend ◽  
Asthma Patients ◽  
And Control ◽  
Experimental Group

In this study eighty bronchial asthma patients were selected through accidental sampling and sample was divided into experimental group (n=40) and control group (n=40). They underwent Yoga therapy program for 60 days with an integrated set of yogic practices, including jihvamula, karnarandhra, kapalrandhra, tadasana, tiryaka tadasana, kati chakrasana and marjariasana , nadishodhan pranayama, bhastrika pranayama and Gayatri mantra. Bronchial asthmatics practiced these exercises for 45 minutes daily for 60 days. Stress level and forced expiratory volume in one second (FEV1) were assessed on all the Bronchial asthmatics, both initially and after completion of study. The obtained data were statistically analyzed through paired t-test. Subjects of experimental group showed a statistically significant decreasing trend in average stress level and increasing trend in average forced expiratory volume in one second (FEV1) after 60 days Yoga practice, while control group showed no such improvement (P>0.01).

scholarly journals PREDICTORS OF EFFECTIVE USE OF T CELL MIMETICS IN IMMUNE AND ENDOCRINE DISORDERS TREATMENT

2019 ◽  
Vol 19 (1S) ◽  
pp. 176-178
Author(s):  
D S Luybimov ◽  
A N Britanov
Keyword(s):  
Immune Response ◽  
Bacterial Infections ◽  
Acute Bronchitis ◽  
Acute Stage ◽  
Endocrine Disorders ◽  
Clinical Markers ◽  
Phagocytic Capacity ◽  
Humoral Immune ◽  
Neurohumoral Regulation ◽  
Cellular Immune

98 infants with acute bronchitis aged 3-36 months showed general basic adaptive response on the acute stage: depression of cellular immune response, active humoral immune response, CIC increase and enhanced phagocytic capacity of neutrophils. ACTH and TSH levels were elevated against depletion of cortisol and triiodothyronine levels. Clinical markers together with immune and endocrine predictive markers of the disease severity, character of complications and comorbidity were revealed. Thymogen is indicated for treatment in case of prevailing of hyperergic disorders both in the central core of neurohumoral regulation and the intra-immune - IgA increase. Thymaline proves effective in hyperergia treatment - thymomegaly with low IgA and cortisol levels along with pre-existing bacterial infections.

scholarly journals The efficiency of non-specific inhaled immunotherapy with ruzam in patients with atopic bronchialasthma

2011 ◽  
Vol 8 (4) ◽  
pp. 49-54
Author(s):  
D V Terekhov ◽  
Nataliya Mikhaylovna Nenasheva ◽  
D V Terekhov ◽  
N M Nenasheva
Keyword(s):  
Blood Serum ◽  
Bronchial Asthma ◽  
Bronchial Hyperresponsiveness ◽  
Allergic Inflammation ◽  
Induced Sputum ◽  
Control Group ◽  
Asthma Control Test ◽  
Open Label ◽  
Atopic Bronchial Asthma ◽  
Markers Of Inflammation

Background. To study the efficacy and safety of inhaled form of ruzam in adult patients with persistent mild and moderate atopic bronchial asthma (ba). Materials and methods. a placebo-controlled prospective, randomized, open label study of an efficacy of ruzam a solution for inhalations (in bottles on 2,5 or 5 ml) on 2,5 ml onсe a day through nebulizer during 2 weeks was conducted in adult asthmatic patients. The efficacy of therapy was evaluated by dynamics of symptoms and requirement in ƒ2-agonists, besides PEfr, spirometry, bronchial hyperresponsiveness (bhr), asthma control test (aCT ), level of the markers of the allergic inflammation were performed. Results. reduction of bа symptoms, increase morning PEfr, decrease of bhr and NОex level in patients who received ruzam compared with control group was observed. achievement of clinical control of bа in patients treated by ruzam was accompanied by statistically significant positive dynamics of markers of inflammation (ifNg, ECP) in blood serum and in supernatant of the induced sputum. it testifies about anti-inflammatory influence of ruzam. Conclusion. inhaled therapy with ruzam in a complex with iNCs basic therapy is an effective and safe method of treatment of persistent atopic bronchial asthma. This combination allows to reach clinical and functional control of ba faster and to reduce inflammation markers in blood serum and induced sputum.

scholarly journals Clinical Relevance of Plasma Concentrations of MBL in Accordance with IgE Levels in Children Diagnosed with Bronchial Asthma

Medicina ◽  
2020 ◽  
Vol 56 (11) ◽  
pp. 594
Author(s):  
Simona Maria Borta ◽  
Simona Dumitra ◽  
Imola Miklos ◽  
Romana Popetiu ◽  
Luminița Pilat ◽  
...  
Keyword(s):  
Bronchial Asthma ◽  
Clinical Relevance ◽  
Severe Disease ◽  
Plasma Concentrations ◽  
Control Group ◽  
Asthma Patients ◽  
Diagnostic Role ◽  
Study Population ◽  
Ige Determination ◽  
And Control

Background and objectives: Bronchial asthma is a heterogeneous, multifactorial pulmonary disease characterized by variable airway obstruction caused by chronic inflammation. Our study investigates the clinical relevance of MBL plasma levels in accordance with IgE values in children who attended a pediatric consult for respiratory symptoms with bronchial asthma. Materials and Methods: The study population consists of patients <18-years-old and included 43 patients with bronchial asthma and 64 age-matched healthy subjects as a control group. We used the ELISA Human MBL Immunoassay kit and the electrochemiluminescence immunoassay (ECLIA) kit for IgE determination. Results: Our results show significantly different distributions of patients in the bronchial asthma group and control group. The measured values were within the normal range for most controls, while the bronchial asthma patients displayed higher values of plasma MBL and IgE levels. We observed a wider heterogeneity in MBL concentrations in bronchial asthma patients when compared to the healthy age-matched controls. Our results also suggest a potential clinical usefulness of plasma MBL concentrations in accordance with IgE and eosinophil cells levels in the diagnosis of bronchial asthma, and our results may suggest a prognostic role of MBL in the evolution of asthmatic disease; however, further studies are necessary to confirm these findings. Conclusions: We can say that plasma MBL concentrations present a relative diagnostic role for bronchial asthma in pediatric patients and may suggest a more severe disease progression; however, further studies are needed to elucidate the role played by MBL in the determination and evolution of this disease.

scholarly journals Relationship of Serum Levels of IL-17, IL-18, TNF-α, and Lung Function Parameters in Patients with COPD, Asthma-COPD Overlap, and Bronchial Asthma

2020 ◽  
Vol 2020 ◽  
pp. 1-11
Author(s):  
Nailya Kubysheva ◽  
Marina Boldina ◽  
Tatyana Eliseeva ◽  
Svetlana Soodaeva ◽  
Igor Klimanov ◽  
...  
Keyword(s):  
Blood Serum ◽  
Airway Obstruction ◽  
Bronchial Asthma ◽  
Systemic Inflammation ◽  
Lung Ventilation ◽  
Serum Levels ◽  
Control Group ◽  
Chronic Obstructive ◽  
Obstructive Pulmonary Disease ◽  
Potential Marker

Determination of markers of systemic inflammation is one of the important directions in the study of pathogenesis and improvement of diagnosis of chronic obstructive pulmonary disease (COPD), asthma-COPD overlap (ACO), and bronchial asthma (BA). The aim of our work was a comparative study of the features of changes in serum levels of IL-17, IL-18, and TNF-α in patients with COPD, ACO, and BA with various severity of the disease, as well as evaluation of the relationship between the level of these cytokines and lung ventilation function. A total of 147 patients with COPD (n=58), ACO (n=57), and BA (n=32) during a stable period have been examined in this study. The control group included 21 healthy nonsmokers with similar sex-age indicators. Serum levels of IL-17, IL-18, and TNF-α were determined by ELISA. The concentrations of these cytokines in the circulation in the studied patients with COPD, ACO, and BA were higher than those in healthy nonsmokers (p≤0.001). IL-17 and IL-18 levels in the blood serum were comparable in all examined patients. The mean TNF-α concentrations in the circulation in COPD and ACO were significantly higher than those in BA (p<0.001). In patients with COPD, the levels of IL-17 and TNF-α increased progressively against the background of a decrease in numerous spirometric indicators, which allows us to consider these cytokines as systemic biomarkers of disease severity. In BA, the inverse correlations between the level of IL-17 and FEV1/FVC (%) and FEV1 have been found. In patients with ACO, the increase in IL-18 levels was associated with a decrease in FEV1 and TNF-α with FEV1/FVC (%). These findings indicate that IL-17, IL-18, and TNF-α can participate in the mechanisms of systemic inflammation and the genesis of disorders of airway obstruction in COPD, AСO, and BA. An increase in the levels of IL-17 and TNF-α may be associated with impaired bronchial patency in COPD and BA. The established associations of the IL-18 concentration in the blood serum and FEV1 only in patients with ACO allow using the level of IL-18 as a potential marker of the degree of impaired airway obstruction in this disease.

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