Imaging Molecular Adhesion in Cell Rolling by Adhesion Footprint Assay

10.3791/63013 ◽  
2021 ◽  
Author(s):  
Scott Minh An ◽  
Seong Ho Kim ◽  
Vanessa J. White ◽  
Adam B. Yasunaga ◽  
Kathleen M. McMahon ◽  
...  
Keyword(s):  
Cell Rolling ◽  
Molecular Adhesion

Molecular adhesion

AccessScience ◽  
2015 ◽  
Keyword(s):  
Molecular Adhesion

scholarly journals Mechanical force effect on the two-state equilibrium of the hyaluronan-binding domain of CD44 in cell rolling

2015 ◽  
Vol 112 (22) ◽  
pp. 6991-6996 ◽  
Author(s):  
Takashi Suzuki ◽  
Miho Suzuki ◽  
Shinji Ogino ◽  
Ryo Umemoto ◽  
Noritaka Nishida ◽  
...  
Keyword(s):  
Shear Stress ◽  
Conformational Change ◽  
Mechanical Force ◽  
Binding Domain ◽  
Terminal Region ◽  
Hematopoietic Progenitor Cell ◽  
High Shear ◽  
Cell Rolling ◽  
High Affinity ◽  
C Terminus

CD44 is the receptor for hyaluronan (HA) and mediates cell rolling under fluid shear stress. The HA-binding domain (HABD) of CD44 interconverts between a low-affinity, ordered (O) state and a high-affinity, partially disordered (PD) state, by the conformational change of the C-terminal region, which is connected to the plasma membrane. To examine the role of tensile force on CD44-mediated rolling, we used a cell-free rolling system, in which recombinant HABDs were attached to beads through a C-terminal or N-terminal tag. We found that the rolling behavior was stabilized only at high shear stress, when the HABD was attached through the C-terminal tag. In contrast, no difference was observed for the beads coated with HABD mutants that constitutively adopt either the O state or the PD state. Steered molecular dynamics simulations suggested that the force from the C terminus disrupts the interaction between the C-terminal region and the core of the domain, thus providing structural insights into how the mechanical force triggers the allosteric O-to-PD transition. Based on these results, we propose that the force applied from the C terminus enhances the HABD–HA interactions by inducing the conformational change to the high-affinity PD transition more rapidly, thereby enabling CD44 to mediate lymphocyte trafficking and hematopoietic progenitor cell homing under high-shear conditions.

Tailoring the Trajectory of Cell Rolling with Cytotactic Surfaces

Langmuir ◽  
2011 ◽  
Vol 27 (24) ◽  
pp. 15345-15351 ◽  
Author(s):  
Collin Edington ◽  
Hironobu Murata ◽  
Richard Koepsel ◽  
Jill Andersen ◽  
Sungeun Eom ◽  
...  
Keyword(s):  
Cell Rolling

scholarly journals Stem cell membrane engineering for cell rolling using peptide conjugation and tuning of cell–selectin interaction kinetics

Biomaterials ◽  
2012 ◽  
Vol 33 (20) ◽  
pp. 5004-5012 ◽  
Author(s):  
Hao Cheng ◽  
Marta Byrska-Bishop ◽  
Cathy T. Zhang ◽  
Christian J. Kastrup ◽  
Nathaniel S. Hwang ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Membrane ◽  
Cell Rolling ◽  
Peptide Conjugation ◽  
Interaction Kinetics ◽  
Membrane Engineering

scholarly journals Complementary electrochemical ICP-MS flow cell and in-situ AFM study of the anodic desorption of molecular adhesion promotors

2021 ◽  
pp. 151015
Author(s):  
Dominik Dworschak ◽  
Carina Brunnhofer ◽  
Markus Valtiner
Keyword(s):  
Flow Cell ◽  
Molecular Adhesion ◽  
Icp Ms

Enhanced corrosion resistance of adhesive/galvanised steel interfaces by nanocrystalline ZnO thin film deposition and molecular adhesion promoting films

2021 ◽  
pp. 1-21
Author(s):  
Richard Grothe ◽  
Jan Striewe ◽  
Dennis Meinderink ◽  
Thomas Tröster ◽  
Guido Grundmeier
Keyword(s):  
Thin Film ◽  
Corrosion Resistance ◽  
Thin Film Deposition ◽  
Film Deposition ◽  
Zno Thin Film ◽  
Molecular Adhesion ◽  
Galvanised Steel

In situ scrutinize the adsorption of sulfamethoxazole in water using AFM force spectroscopy: molecular adhesion force determination and fractionation

2021 ◽  
pp. 128128
Author(s):  
Jiayi Wu ◽  
Rui Wang ◽  
Yuyao Zhang ◽  
Baoliang Chen ◽  
Xiaoying Zhu
Keyword(s):  
Adhesion Force ◽  
Force Spectroscopy ◽  
Molecular Adhesion

scholarly journals Roles of cell and microvillus deformation and receptor-ligand binding kinetics in cell rolling

2008 ◽  
Vol 295 (4) ◽  
pp. H1439-H1450 ◽  
Author(s):  
Parag Pawar ◽  
Sameer Jadhav ◽  
Charles D. Eggleton ◽  
Konstantinos Konstantopoulos
Keyword(s):  
Ligand Binding ◽  
Three Dimensional ◽  
Theoretical Models ◽  
Binding Kinetics ◽  
Cell Deformation ◽  
Receptor Ligand ◽  
Length Scales ◽  
Bond Behavior ◽  
Cell Rolling ◽  
Threshold Phenomenon

Polymorphonuclear leukocyte (PMN) recruitment to sites of inflammation is initiated by selectin-mediated PMN tethering and rolling on activated endothelium under flow. Cell rolling is modulated by bulk cell deformation (mesoscale), microvillus deformability (microscale), and receptor-ligand binding kinetics (nanoscale). Selectin-ligand bonds exhibit a catch-slip bond behavior, and their dissociation is governed not only by the force but also by the force history. Whereas previous theoretical models have studied the significance of these three “length scales” in isolation, how their interplay affects cell rolling has yet to be resolved. We therefore developed a three-dimensional computational model that integrates the aforementioned length scales to delineate their relative contributions to PMN rolling. Our simulations predict that the catch-slip bond behavior and to a lesser extent bulk cell deformation are responsible for the shear threshold phenomenon. Cells bearing deformable rather than rigid microvilli roll slower only at high P-selectin site densities and elevated levels of shear (≥400 s−1). The more compliant cells (membrance stiffness = 1.2 dyn/cm) rolled slower than cells with a membrane stiffness of 3.0 dyn/cm at shear rates >50 s−1. In summary, our model demonstrates that cell rolling over a ligand-coated surface is a highly coordinated process characterized by a complex interplay between forces acting on three distinct length scales.

Role of cytoskeleton and deformability in laminin-mediated cell rolling

Biorheology ◽  
1998 ◽  
Vol 35 (1) ◽  
pp. 37-51
Author(s):  
S Wu
Keyword(s):  
Cell Rolling

Molecular adhesion development in a neural cell monolayer forming in an ultrasound trap

2005 ◽  
Vol 22 (3) ◽  
pp. 229-240 ◽  
Author(s):  
Despina Bazou ◽  
George A. Foster ◽  
James R. Ralphs ◽  
W. Terence Coakley
Keyword(s):  
Cell Monolayer ◽  
Neural Cell ◽  
Molecular Adhesion
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