scholarly journals USO DE BIOAGENTES NA SUPRESSÃO DA Macrophomina phaseolina NA CULTURA DO FEIJOEIRO COMUM

2021 ◽  
Vol 5 (1) ◽  
pp. 1-11
Author(s):  
Geana Jesus Caixeta ◽  
Lucas Marquezan Nascimento ◽  
Marta Cristina Corsi de Filippi ◽  
Fábio José Gonçalves ◽  
Alan Carlos Alves de Souza
Keyword(s):  
Pseudomonas Fluorescens ◽  
Trichoderma Harzianum ◽  
Macrophomina Phaseolina ◽  
Bacillus Sp

A Macrophomina phaseolina causa a doença podridão-cinzenta-do-caule na cultura do feijoeiro e leva a perdas significativas do grão devido às dificuldades para o controle. Diante disso, o objetivo deste trabalho foi avaliar o efeito do uso de Trichoderma e de rizobactérias como bioagentes na supressão da doença na cultura do feijoeiro comum. O experimento foi realizado em condição de telado e adotou-se o delineamento em blocos inteiramente casualizados. Foram realizados seis tratamentos com oito repetições. Os tratamentos consistiram em: T1– Testemunha; T2 – Pseudomonas fluorescens; T3 – Burkholderia pyrrocinia; T4 – Bacillus sp.; T5 – Trichodermil 1306® e T6 – Rancona T®. Os tratamentos foram aplicados via semente e pulverização foliar aos 14 e 21 dias após o plantio. O fitopatógeno Macrophomina phaseolina foi inoculado aos 21 dias após o plantio, utilizando o método de palitos-de-dente. A avaliação da severidade da doença e da área abaixo da curva de progresso da doença (AACPD) foi realizada aos 1, 2, 4 e 8 dias após a inoculação, por meio de uma escala de notas descritivas. A avaliação da promoção de crescimento foi realizada aos 21 dias após o plantio. As plantas de feijoeiro que tiveram o tratamento de sementes e a pulverização foliar com as rizobactérias P. fluorescens e Bacillus sp. se sobressaíram significativamente, aumentando a biomassa da parte aérea em 46,73% e 41,43%, respectivamente, em comparação a testemunha. As plantas tratadas via sementes e via pulverização foliar com a rizobactéria Bacillus sp. apresentaram menor índice de severidade da doença, com supressão de 51,67% em relação a testemunha. Segundo AACPD, o tratamento que apresentou menor área com presença de plantas doentes foi o tratamento com a bactéria Bacillus sp. com 34,58 %. De acordo com os resultados obtidos, a utilização dos bioagentes Bacillus sp. e Trichoderma harzianum se mostraram eficientes na supressão da Macrophomina phaseolina na cultura do feijoeiro comum. A utilização de Pseudomonas fluorescens e o Bacillus sp. são eficientes na promoção de crescimento de biomassa de parte aérea.

scholarly journals Using maize wastes, fermented by co-cultures of Trichoderma harzianum and Pseudomonas fluorescens, as grain dressing against m maize diseases under field conditions

2020 ◽  
Vol 30 (1) ◽  
Author(s):  
Sherien M. M. Atalla ◽  
Mokhtar M. Abdel-Kader ◽  
Nadia G. El-Gamal ◽  
Nehal S. El-Mougy
Keyword(s):  
Pseudomonas Fluorescens ◽  
Trichoderma Harzianum ◽  
Raw Materials ◽  
Chitosan Nanoparticles ◽  
Field Conditions ◽  
Optimum Ratio ◽  
Transmission Electron ◽  
Environmentally Safe ◽  
The Mean ◽  
Uv Visible

Abstract Maize (Zea mays L.) is one of the most economic crops in Egypt. Production of amylase from the waste of maize is the most economic and cheap renewable and most abundant raw materials present in environment. Biosynthesis of Cu-chitosan nanoparticles for amylase production by co-culturing between Trichoderma harzianum and Pseudomonas fluorescens at different ratios compared to free conditions was the main purpose of this study. The optimum ratio 8:2, recorded between P. fluorescens: T. harzianum, was the most promising for production of amylase produce 22.47 and 28.60 U/ml for free and nano, respectively. The UV visible spectral analysis Cu-chitosan NPs was 220 nm, while the mean diameter, using transmission electron microscopy was 0.5 μm. Application of fermented maize wastes by co-cultivation of P. fluorescence and T. harzianum, as a grain dressing before sowing declared the reduction in both root and foliar diseases during the maize growing season, starting from germination up to 70 days of its vegetative growth under field conditions. A promising approach is the creation and use of environmentally safe products, whose protective effect is based on the induction of hydrolase inhibitors in plants.

scholarly journals PENEKANAN HAYATI PENYAKIT MOLER PADA BAWANG MERAH DENGAN TRICHODERMA HARZIANUM, TRICHODERMA KONINGII, DAN PSEUDOMONAS FLUORESCENS P60

2007 ◽  
Vol 7 (1) ◽  
pp. 53-61
Author(s):  
Suprapto Edy Santoso ◽  
Loekas Soesanto ◽  
Totok Agung Dwi Haryanto
Keyword(s):  
Population Density ◽  
Pseudomonas Fluorescens ◽  
Incubation Period ◽  
Trichoderma Harzianum ◽  
Block Design ◽  
Research Result ◽  
Pathogen Population ◽  
Trichoderma Koningii ◽  
Spraying Method ◽  
Plot Design

Biological Suppression of Moler Disease on Shallot  by Trichoderma harzianum, Trichoderma koningii, and Pseudomonas fluorescens P60. Research aiming for (1) knowing efectivity of biological suppression with T. harzianum, T. koningii, and P. fluorescens P60 and (2) studying growth and production of shallot caused by the suppression carried out at the shallot farm. Split-Split Plot Design arranged in Randomized Completely Block Design was used with three replicates. The research result showed that P. fluorescens P60 was the most effective antagonistic agent to suppress the disease either alone or in combination while T. harzianumi and T. koningii did not suppress effectively. Pseudomonas fluorescens P60 could suppress the disease up to 41.96%. The best method of P. fluorescens P60 application was spraying method for 10 mL with 107 cfu/mL population density, which was able to dicrease incubation period, disease intensity, and final pathogen population up to 62.46, 18.19, and 80.67%, respectively. Growth and production of the crop tended to increase resulted from biological suppression by P. fluorescens P60, but not by T. harzianum nor T. koningii.

Assessing synergism of combined applications of Trichoderma harzianum and Pseudomonas fluorescens to control blast and bacterial leaf blight of rice

2018 ◽  
Vol 152 (3) ◽  
pp. 747-757 ◽  
Author(s):  
Prashant P. Jambhulkar ◽  
Pratibha Sharma ◽  
Raja Manokaran ◽  
Dilip K. Lakshman ◽  
Pramod Rokadia ◽  
...  
Keyword(s):  
Pseudomonas Fluorescens ◽  
Trichoderma Harzianum ◽  
Leaf Blight ◽  
Bacterial Leaf Blight

scholarly journals Evaluation of Bacterial Antagonists for Biological Control of Broccoli Head Rot Caused by Pseudomonas fluorescens

2006 ◽  
Vol 96 (4) ◽  
pp. 408-416 ◽  
Author(s):  
Xiaohui Cui ◽  
Rob Harling
Keyword(s):  
Biological Control ◽  
Quorum Sensing ◽  
Pseudomonas Fluorescens ◽  
Virulence Gene ◽  
Pathogenicity Test ◽  
Bacillus Sp ◽  
Inhibitory Effects ◽  
Extracellular Metabolite ◽  
Head Rot ◽  
Tn5 Mutant

Pectolytic strains of Pseudomonas fluorescens are opportunistic pathogens of broccoli, causing head rot in temperate regions of the world. In this study, we investigated the potential of two bacterial isolates, P. fluorescens m6418 and Bacillus sp. A24, for biological control of broccoli head rot caused by P. fluorescens 5064, isolated from diseased broccoli in Scotland, UK. P. fluorescens m6418, a Tn5 mutant of wild-type 5064, is nonpathogenic and overproduces an extracellular metabolite with strong antimicrobial activity. In this study, we identified the anti-microbial metabolite produced by strain m6418 as pyrrolnitrin. P. fluorescens m6418 had significant inhibitory effects against strain 5064 both in culture and on broccoli leaves. In an excised broccoli head pathogenicity test, strain m6418, when coinoculated with P. fluorescens 5064, reduced disease by 41%. Bacillus sp. A24 produces an enzyme that can degrade N-acyl homoserine lactones, signaling molecules employed by bacteria for quorum sensing. Bacillus sp. A24 was capable of out-competing P. fluorescens 5064 when grown together in culture, and could degrade the quorum sensing signal of P. fluorescens 5064 (and thereby attenuate its virulence gene production). However, Bacillus sp. A24 had only a limited biocontrol effect on P. fluorescens 5064 in the excised broccoli head assay.

scholarly journals Effectiveness of some microorganisms in the limitation of grapevine cuttings infection by Phomopsis viticola Sacc.

2013 ◽  
Vol 57 (1-2) ◽  
pp. 109-118 ◽  
Author(s):  
Ewa Król
Keyword(s):  
Pseudomonas Fluorescens ◽  
Bacillus Sp ◽  
Antagonistic Fungi ◽  
Phomopsis Viticola

The possibilities of using antagonistic fungi and bacteria in the limitation of grapevine stems infection by <i>Phomopsis viticola</i> Sacc. were studied. <i>Trichodema koningii</i> Oud., <i>T.viride</i> Persoon ex S.F.,<i>T.harzianum Rifai, Gliocladium catenulatum</i> Gilman and Abbott, <i>G.fimbriatum</i> Gilman and Abbott, Bacillus sp., <i>Pseudomonas fluorescens</i> and five unidentified isolates of bacteria i.e.: 22a, 35, 40, 45, 66 were estimated. It was appeared what <i>Trichoderma</i> spp. were the most effective in protection of grapevine stems against the infection by <i>P.viticola</i>. After these antagonistic fungi were used on protected grapevine canes not numerous necrosis were observed and few cultures of pathogen were reisolated from them. Moreover, <i>Trichoderma</i> spp. survived on the grapevine stems during the period of experiment. The abilities of other microorganisms tested to protect grapevine cuttings against <i>P.viticola</i> infection and to exist on the stems were less than <i>Trichoderma</i> spp.

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