Effectiveness of some microorganisms in the limitation of grapevine cuttings infection by Phomopsis viticola Sacc.

The possibilities of using antagonistic fungi and bacteria in the limitation of grapevine stems infection by Phomopsis viticola Sacc. were studied. Trichodema koningii Oud., T.viride Persoon ex S.F.,T.harzianum Rifai, Gliocladium catenulatum Gilman and Abbott, G.fimbriatum Gilman and Abbott, Bacillus sp., Pseudomonas fluorescens and five unidentified isolates of bacteria i.e.: 22a, 35, 40, 45, 66 were estimated. It was appeared what Trichoderma spp. were the most effective in protection of grapevine stems against the infection by P.viticola. After these antagonistic fungi were used on protected grapevine canes not numerous necrosis were observed and few cultures of pathogen were reisolated from them. Moreover, Trichoderma spp. survived on the grapevine stems during the period of experiment. The abilities of other microorganisms tested to protect grapevine cuttings against P.viticola infection and to exist on the stems were less than Trichoderma spp.

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Evaluation of Bacterial Antagonists for Biological Control of Broccoli Head Rot Caused by Pseudomonas fluorescens

Phytopathology ◽

10.1094/phyto-96-0408 ◽

2006 ◽

Vol 96 (4) ◽

pp. 408-416 ◽

Cited By ~ 5

Author(s):

Xiaohui Cui ◽

Rob Harling

Keyword(s):

Biological Control ◽

Quorum Sensing ◽

Pseudomonas Fluorescens ◽

Virulence Gene ◽

Pathogenicity Test ◽

Bacillus Sp ◽

Inhibitory Effects ◽

Extracellular Metabolite ◽

Head Rot ◽

Tn5 Mutant

Pectolytic strains of Pseudomonas fluorescens are opportunistic pathogens of broccoli, causing head rot in temperate regions of the world. In this study, we investigated the potential of two bacterial isolates, P. fluorescens m6418 and Bacillus sp. A24, for biological control of broccoli head rot caused by P. fluorescens 5064, isolated from diseased broccoli in Scotland, UK. P. fluorescens m6418, a Tn5 mutant of wild-type 5064, is nonpathogenic and overproduces an extracellular metabolite with strong antimicrobial activity. In this study, we identified the anti-microbial metabolite produced by strain m6418 as pyrrolnitrin. P. fluorescens m6418 had significant inhibitory effects against strain 5064 both in culture and on broccoli leaves. In an excised broccoli head pathogenicity test, strain m6418, when coinoculated with P. fluorescens 5064, reduced disease by 41%. Bacillus sp. A24 produces an enzyme that can degrade N-acyl homoserine lactones, signaling molecules employed by bacteria for quorum sensing. Bacillus sp. A24 was capable of out-competing P. fluorescens 5064 when grown together in culture, and could degrade the quorum sensing signal of P. fluorescens 5064 (and thereby attenuate its virulence gene production). However, Bacillus sp. A24 had only a limited biocontrol effect on P. fluorescens 5064 in the excised broccoli head assay.

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Biodegradación estimulada de los suelos contaminados con pesticidas organoclorados

Revista de Investigación Agraria y Ambiental ◽

10.22490/21456453.1843 ◽

2017 ◽

Vol 8 (1) ◽

pp. 119-130

Author(s):

María Kopytko ◽

Sandra Natalia Correa-Torres ◽

Martha Jhoana Estévez- Gómez

Keyword(s):

Pseudomonas Fluorescens ◽

Burkholderia Cepacia ◽

Bacillus Sp

DDT al igual que otros pesticidas organoclorados han sido utilizados extensivamente en Colombia entre los años 1970 y 1980 en cultivos de algodón. DDT es resistente a la degradación y fuertemente relacionado con numerosos problemas de salud y actualmente está prohibido su uso. El propósito de esta investigación fue evaluar la biodegradación de DDT y de los productos DDD y DDE, presentes en el suelo contaminado durante 16 años en le municipio Agustín Codazzi, Cesar, Colombia. Se llevaron a cabo bioestimulación, bioaumentación y un tratamiento de control bajo condiciones anaerobias y aerobias secuenciales. El proceso de biodegradación anaerobia duró 8 semanas y consecutivamente se realizó el proceso aerobio por 20 semanas. En el proceso de bioestimulación se adicionó fósforo para optimizar la proporción C:N:P en el suelo tratado. De las bacterias nativas se aislaron las cepas bacterianas con la capacidad biodegradadora del DDT y fueron identificadas mediante las características morfológicas y ampliación por PCR de la región de 1465 pb del gen ribosomal 16S y secuenciación. De estas se seleccionaron cuatro cepas bacterianas para el proceso de bioaumentación: Burkholderia cepacia, Pseudomonas fluorescens, Aeromona caviae, y Bacillus sp, las cuales fueron bioaumentadas en el laboratorio e inoculadas en el suelo tratado en concentración de 108 UFC/ml. La concentración de DDT, DDD y DDE se determinó por medio de cromatografía de gases. Los mejores resultados se obtuvieron durante la fase anaerobia logrando una remoción de DDT, DDD y DDE en un 56,2%, 17,1% y 44,5% respectivamente.

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Population Dynamics of Bacillus sp. L324-92R12 and Pseudomonas fluorescens 2-79RN10 in the Rhizosphere of Wheat

Phytopathology ◽

10.1094/phyto.1997.87.5.559 ◽

1997 ◽

Vol 87 (5) ◽

pp. 559-564 ◽

Cited By ~ 23

Author(s):

Dal-Soo Kim ◽

David M. Weller ◽

R. James Cook

Keyword(s):

Winter Wheat ◽

Pseudomonas Fluorescens ◽

Root Rot ◽

Resistant Mutant ◽

Gaeumannomyces Graminis ◽

Growth Chamber ◽

Bacillus Sp ◽

Population Sizes ◽

Chamber Studies ◽

Growth Chamber Studies

Bacillus sp. L324-92 is suppressive to three root diseases of wheat, namely take-all caused by Gaeumannomyces graminis var. tritici, Rhizoctonia root rot caused by Rhizoctonia solani AG8, and Pythium root rot caused by several Pythium species. Populations of strain L324-92R12, a rifampicin-resistant mutant of L324-92 applied as a seed treatment, were monitored in the rhizosphere and spermosphere of wheat and compared with populations of Pseudomonas fluorescens 2-79RN10, a known, rhizosphere-competent, biocontrol agent. In growth chamber studies, the population sizes of L324-92R12 on roots of wheat were approximately 1,000-fold smaller than those of 2-79RN10 at 5 days after planting, but, thereafter, they increased while those of 2-79RN10 decreased until the two were equal in size at 45 days after planting. In the field with winter wheat, the population sizes of L324-92R12 on roots were at least 10-fold smaller than those of 2-79RN10 during the fall (November 1993) and early spring (March 1994). Thereafter, the population of L324-92R12 remained constant or increased slightly, while the population of 2-79RN10 decreased until the two were roughly the same at 104 to 105 CFU/plant over the period of 150 days (April 1994) until 285 days (harvest) after planting. In growth chamber studies, strain L324-92R12 remained confined to root sections within 3.5 cm below the seed, whereas 2-79RN10 was recovered from all root sections ranging from 0.5 to 6.5 cm below the seed. In the field on winter wheat, both strains were recovered from root sections down to 5.0 to 6.5 cm below the seed at 75 days after planting (mid December), but only 2-79RN10 was recovered at this depth at 90 days after planting. Both strains were recovered from the seed remnants 6 months after planting in the field. Both strains also were recovered from inside the roots and shoots, but population sizes of strain 279RN10 were greater than those of L324 92R12.

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Biomatriconditioning Benih dengan Rizobakteri untuk Meningkatkan Pertumbuhan dan Hasil Tanaman Sorgum (Sorghum bicolor L.)

Agrotekma Jurnal Agroteknologi dan Ilmu Pertanian ◽

10.31289/agr.v4i1.2949 ◽

2019 ◽

Vol 4 (1) ◽

pp. 52

Author(s):

Fitrianti Handayani ◽

Gusti Ayu Kade Sutariati ◽

Abdul Madiki

Keyword(s):

Sorghum Bicolor ◽

Pseudomonas Fluorescens ◽

Animal Husbandry ◽

Dry Weight ◽

Growth And Yield ◽

Bacillus Sp ◽

Rice Hulls ◽

Brick Powder ◽

Sorghum Bicolor L ◽

Range Test

<h1>The experiment was aimed to know the effect of seed biomatriconditioning with rhizobacteria to improve growth and yield of sorghum (Sorghum bicolor L.). The experiment was conducted in the Field of Animal Husbandry Agriculture Faculty, Haluoleo University from August up to November 2010. The experiment was arranged based on randomized completely design (CRD) which consisted of 5 seed biomatriconditioning with rhizobacteria treatments, namely: without seed biomatriconditioning (M0), seed biomatriconditioning with Bacillus sp. CKD061 + rice hulls charcoal (M1), seed biomatriconditioning with Bacillus sp. CKD061 + red brick powder (M2), seed biomatriconditioning with Pseudomonas fluorescens PG01 + rice hulls charcoal (M3), and seed biomatriconditioning with Pseudomonas fluorescens PG01 + red brick powder (M4). Every treatment was replicated 3 times, therefore, overall there were 15 experimental units. Data obtained were analized using analysis of variance and followed with Duncan’s Multiple Range Test. The result showed that seed treatment with biomatriconditioning using rhizobacteria gave significant effect in improving growth and yield of sorghum. Seed biomatriconditioning with sp. Bacillus CKD061 + red brick powder was the most effective treatment in increasing plant height, leaf area, dry weight of plant bomass, grain weight, and 1000 seed weight of sorghum.</h1>

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El papel de los procariotas en la degradación del cianuro

Herreriana ◽

10.29057/h.v3i2.8162 ◽

2022 ◽

Vol 3 (2) ◽

pp. 33-36

Author(s):

Sylvia Martinez ◽

Andrea Aguilar Hernández

Keyword(s):

Escherichia Coli ◽

Pseudomonas Fluorescens ◽

Bacillus Sp

El cianuro es una sustancia tóxica, potencialmente letal, cuya concentración ha incrementado debido a las actividades antropogénicas, lo que genera un impacto negativo en el ecosistema y en la salud de los organismos expuestos. Desde hace un par de décadas se ha utilizado un proceso biotecnológico llamado “biorremediación”, que consiste en aprovechar las capacidades metabólicas de los microorganismos como bacterias y arqueas que degradan o atenúan contaminantes como el cianuro y los compuestos que forma. El presente trabajo tiene como objetivo identificar a las principales especies de bacterias y arqueas con capacidad para degradar el cianuro en sus distintas formas mediante un análisis bibliográfico. Dentro de los microorganismos utilizados para la degradación de cianuro se encuentran Bacillus sp., Escherichia coli, Pseudomonas fluorescens, entre otras especies. Con base en el número de especies descritas y sus eficientes tasas de remoción, se determinó que el proceso de biorremediación del cianuro es una alternativa prometedora, además existe una mayor degradación en el proceso aeróbico comparado con el proceso anaeróbico.

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Optimization of inorganic phosphate solubilization by Pseudomonas fluorescens and Bacillus sp. isolated from wheat rhizospheric soil

International Journal of Biosciences (IJB) ◽

10.12692/ijb/10.4.142-150 ◽

2017 ◽

Vol 10 (4) ◽

pp. 142-150 ◽

Cited By ~ 2

Keyword(s):

Pseudomonas Fluorescens ◽

Inorganic Phosphate ◽

Phosphate Solubilization ◽

Bacillus Sp ◽

Rhizospheric Soil

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USO DE BIOAGENTES NA SUPRESSÃO DA Macrophomina phaseolina NA CULTURA DO FEIJOEIRO COMUM

Ipê Agronomic Journal ◽

10.37951/2595-6906.2021v5i1.6881 ◽

2021 ◽

Vol 5 (1) ◽

pp. 1-11

Author(s):

Geana Jesus Caixeta ◽

Lucas Marquezan Nascimento ◽

Marta Cristina Corsi de Filippi ◽

Fábio José Gonçalves ◽

Alan Carlos Alves de Souza

Keyword(s):

Pseudomonas Fluorescens ◽

Trichoderma Harzianum ◽

Macrophomina Phaseolina ◽

Bacillus Sp

A Macrophomina phaseolina causa a doença podridão-cinzenta-do-caule na cultura do feijoeiro e leva a perdas significativas do grão devido às dificuldades para o controle. Diante disso, o objetivo deste trabalho foi avaliar o efeito do uso de Trichoderma e de rizobactérias como bioagentes na supressão da doença na cultura do feijoeiro comum. O experimento foi realizado em condição de telado e adotou-se o delineamento em blocos inteiramente casualizados. Foram realizados seis tratamentos com oito repetições. Os tratamentos consistiram em: T1– Testemunha; T2 – Pseudomonas fluorescens; T3 – Burkholderia pyrrocinia; T4 – Bacillus sp.; T5 – Trichodermil 1306® e T6 – Rancona T®. Os tratamentos foram aplicados via semente e pulverização foliar aos 14 e 21 dias após o plantio. O fitopatógeno Macrophomina phaseolina foi inoculado aos 21 dias após o plantio, utilizando o método de palitos-de-dente. A avaliação da severidade da doença e da área abaixo da curva de progresso da doença (AACPD) foi realizada aos 1, 2, 4 e 8 dias após a inoculação, por meio de uma escala de notas descritivas. A avaliação da promoção de crescimento foi realizada aos 21 dias após o plantio. As plantas de feijoeiro que tiveram o tratamento de sementes e a pulverização foliar com as rizobactérias P. fluorescens e Bacillus sp. se sobressaíram significativamente, aumentando a biomassa da parte aérea em 46,73% e 41,43%, respectivamente, em comparação a testemunha. As plantas tratadas via sementes e via pulverização foliar com a rizobactéria Bacillus sp. apresentaram menor índice de severidade da doença, com supressão de 51,67% em relação a testemunha. Segundo AACPD, o tratamento que apresentou menor área com presença de plantas doentes foi o tratamento com a bactéria Bacillus sp. com 34,58 %. De acordo com os resultados obtidos, a utilização dos bioagentes Bacillus sp. e Trichoderma harzianum se mostraram eficientes na supressão da Macrophomina phaseolina na cultura do feijoeiro comum. A utilização de Pseudomonas fluorescens e o Bacillus sp. são eficientes na promoção de crescimento de biomassa de parte aérea.

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Kemampuan Campuran Bacillus sp., Pseudomonas fluorescens, dan Trichoderma sp. untuk Mengendalikan Penyakit Layu Bakteri pada Tanaman Tomat

Jurnal Fitopatologi Indonesia ◽

10.14692/jfi.14.2.63 ◽

2018 ◽

Vol 14 (2) ◽

pp. 63

Author(s):

Abdul Manan ◽

Endang Mugiastuti ◽

Loekas Soesanto

Keyword(s):

Plant Growth ◽

Pseudomonas Fluorescens ◽

Bacterial Wilt ◽

Disease Incidence ◽

Growth And Yield ◽

Bacillus Sp ◽

Tomato Production ◽

Potential Control ◽

Trichoderma Sp ◽

Tomato Bacterial Wilt

Ability of Bacillus sp., Pseudomonas fluorescens, and Trichoderma sp. to Control Tomato Bacterial Wilt DiseaseRalstonia solanacearum is known as the causal agent of bacterial wilt on tomato. The bacteria may infect all stadia of plant growth and decrease tomato production. Biological control using antagonistic microbes is considered as a potential control alternative for the disease. This research was aimed to assay the ability of combination treatment of Bacillus sp., Pseudomonas fluorescens, and Trichoderma sp. in controlling R. solanacearum and its effect on growth and yield of tomato in the field. The treatments consisted of control (without antagonistic microbes), mixed combination of Bacillus sp. B8 + Bacillus sp. B11 + Trichoderma sp.; Bacillus sp. B8 + Pseudomonas flourescens P8 + Trichoderma sp.; and Streptomisin sulfat 20%. Antagonistic microbes was applied at planting time, as much as 100 mL per plant. The result showed that mixed combination of Bacillus sp. B8 + Bacillus sp. B11 + Trichoderma sp. was the best treatment in controlling the disease as indicated by delaying incubation period up to 6.2 days, decreasing disease incidence up to 12.3%, increasing plant growth up to 42.80%, and increasing yield up to 14.99%.

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