scholarly journals PENEKANAN HAYATI PENYAKIT MOLER PADA BAWANG MERAH DENGAN TRICHODERMA HARZIANUM, TRICHODERMA KONINGII, DAN PSEUDOMONAS FLUORESCENS P60

2007 ◽  
Vol 7 (1) ◽  
pp. 53-61
Author(s):  
Suprapto Edy Santoso ◽  
Loekas Soesanto ◽  
Totok Agung Dwi Haryanto
Keyword(s):  
Population Density ◽  
Pseudomonas Fluorescens ◽  
Incubation Period ◽  
Trichoderma Harzianum ◽  
Block Design ◽  
Research Result ◽  
Pathogen Population ◽  
Trichoderma Koningii ◽  
Spraying Method ◽  
Plot Design

Biological Suppression of Moler Disease on Shallot  by Trichoderma harzianum, Trichoderma koningii, and Pseudomonas fluorescens P60. Research aiming for (1) knowing efectivity of biological suppression with T. harzianum, T. koningii, and P. fluorescens P60 and (2) studying growth and production of shallot caused by the suppression carried out at the shallot farm. Split-Split Plot Design arranged in Randomized Completely Block Design was used with three replicates. The research result showed that P. fluorescens P60 was the most effective antagonistic agent to suppress the disease either alone or in combination while T. harzianumi and T. koningii did not suppress effectively. Pseudomonas fluorescens P60 could suppress the disease up to 41.96%. The best method of P. fluorescens P60 application was spraying method for 10 mL with 107 cfu/mL population density, which was able to dicrease incubation period, disease intensity, and final pathogen population up to 62.46, 18.19, and 80.67%, respectively. Growth and production of the crop tended to increase resulted from biological suppression by P. fluorescens P60, but not by T. harzianum nor T. koningii.

scholarly journals PENGIMBASAN KETAHANAN BIBIT PISANG AMBON KUNING TERHADAP PENYAKIT LAYU FUSARIUM DENGAN BEBERAPA JAMUR ANTAGONIS

2009 ◽  
Vol 9 (2) ◽  
pp. 130-140
Author(s):  
Loekas Soesanto ◽  
Ruth Feti Rahayuniati
Keyword(s):  
Population Density ◽  
Incubation Period ◽  
Disease Severity ◽  
Induced Resistance ◽  
Fusarium Wilt ◽  
Block Design ◽  
Antagonistic Fungi ◽  
Trichoderma Koningii ◽  
Randomized Block Design ◽  
Induce Resistance

Induced resistance of Ambon Kuning cultivar banana seedling to fusarium wilt with antagonistic fungi.  A research aiming at knowing the effect of antagonistic fungi supernatant on banana induced resistance, Fusarium wilt development, and banana growth was carried out from July up to December 2008. Randomized Block Design was used with four replicates. Treatments tested were control, with supernatant of Gliocladium virens, Trichoderma harzianum isolated from banana, ginger, and ginseng, Trichoderma koningii, and Fusarium equiseti, applied by injection to banana seedling corm and soaked for five minutes. Variables observed were incubation period, disease severity, Foc population density, germination inhibition, growth component, phenolic compound content, and supporting component. Result of the research indicated that the supernatant of G. virens, T. harzianum, and T. koningii could significantly induce resistance of the seedling showed by increasing the phenolic content such as glycoside, saponin, and tannin. The supernatant of all antagonistic fungi could effectively control the disease showed by lengthening incubation period as 48.71%, decreasing the disease severity as 53.57%, decreasing infection rate as 61.48%, increasing the antagonistic effectivity as 51.26%, decreasing the late population density as 45.35%, and decreasing the inoculum inside the plant as 60-100%. The extract could improve the seedling growth.

scholarly journals POTENSI AGENSIA HAYATI DAN NABATI DALAM MENGENDALIKAN PENYAKIT BUSUK RIMPANG JAHE

2005 ◽  
Vol 5 (1) ◽  
pp. 50-57
Author(s):  
Loekas Soesanto ◽  
Sudarmono Sudarmono ◽  
Nur Prihatiningsih ◽  
Abdul Manan ◽  
Endang Iriani ◽  
...  
Keyword(s):  
Incubation Period ◽  
Infection Rate ◽  
Trichoderma Harzianum ◽  
Block Design ◽  
Central Java ◽  
Randomized Block Design ◽  
Ginger Rhizome ◽  
Number Of Leaves ◽  
Central Java Province ◽  
Rhizome Rot

Potency of biological and botanical agents in controlling ginger rhizome rot. Research aimed at knowing integrity of various control agents in controlling causal agent of ginger rhizome rot. The research was carried out at Gowak Village, Pringsurat District, Temanggung Regency, Central Java Province, from November up to May 2004. Randomized Block Design was used with treatments of biological (Bacillus sp. with density of x  1010 cfu ml-1 suspension and Trichoderma harzianum 20 g crop-1), of botanical (clove leaves powder of 20 g crop-1 and neem seeds extract of 25 ml crop-1), and synthetical agents (benomil with concentration of 2 g l-1) either alone or in combination, with three replicates. Variables observed were incubation period, disease intensity, infection rate, number of leaves per crop, crop height, and ginger production per hectare. Result of the research showed that the location was endemic of the disease. The treatments could generally retard the incubation period and the infection rate, decrease the disease intensity, and increase the ginger production. The highest ginger yield was obtained from the plot given Trichoderma harzianum (Trikonis), either alone or in combination with the dry clove leaves of 10 g crop-1 as many as four times with interval of seven days started from seedling planted.

scholarly journals Growth Inhibition of Colletotrichum gloeosporioides by Trichoderma harzianum, Trichoderma koningii, Bacillus subtilis and Pseudomonas fluorescens

1970 ◽  
Vol 29 (1) ◽  
Author(s):  
Febrilia Nur ‘Aini ◽  
Sri Sukamto ◽  
Dwi Wahyuni ◽  
Risma Galuh Suhesti ◽  
Qurrotun Ayunin
Keyword(s):  
Bacillus Subtilis ◽  
Growth Inhibition ◽  
Pseudomonas Fluorescens ◽  
Trichoderma Harzianum ◽  
Colletotrichum Gloeosporioides ◽  
Yield Loss ◽  
Crop Protection ◽  
Key Words Growth ◽  
Trichoderma Koningii ◽  
Significant Yield

Colletotrichum  gloeosporioides is  a  disease  which  can  cause  significant yield  loss  of  cocoa.  The  objective  of  this  research  is  to  investigate  the  abilityof  antagonist  microbes,  Trichoderma  harzianum,  Trichoderma  koningii,  Bacillus subtilis  and Pseudomonas  fluorescens  in  controlling  gloeosporioides  biologically  in  laboratorium  condition.  The  experiment  was  carried  out  in  Crop  Protection  Laboratory,  Indonesian  Coffee  and  Cocoa  Research  Institute.  Results of  this  research  showed  that  antagonist  fungi,  T.  harzianum,  T.  koningii,  had  a stronger  ability  in  inhibiting  growth  of  C.  gloeosporioides about  83%  compared  to  the  ability  of  antagonist  bacteria,  B.  subtilis  and P.  fluorescens,  only about  49%. Key words: Growth  inhibition,  Colletotrichum  gloeosporioides,  Trichoderma  harzianum, Trichoderma koningii,  Bacillus subtilis, Pseudomonas fluorescens.

scholarly journals Gljive i bakterije u biološkoj kontroli uzročnika bolesti biljaka

2019 ◽  
Vol 42 (4) ◽  
pp. 38-43
Author(s):  
Magdalena Matić ◽  
Tamara Siber
Keyword(s):  
Bacillus Subtilis ◽  
Bacillus Thuringiensis ◽  
Rhizoctonia Solani ◽  
Pseudomonas Fluorescens ◽  
Trichoderma Harzianum ◽  
Trichoderma Viride ◽  
Helminthosporium Solani ◽  
Trichoderma Koningii

Zbog stalnog povećanja upotrebe kemijskih pesticida i njihovog štetnog djelovanja na ljudsko zdravlje i okoliš, sve veće zanimanje pobuđuju metode biološke kontrole uzročnika biljnih bolesti. Biološka kontrola je mjera koja podrazumijeva korištenje mikroorganizama koji smanjuju populaciju štetnika i patogena i na taj način štite biljku i kontroliraju razvoj bolesti. U Hrvatskoj je trenutno na tržištu prisutan samo jedan biofungicid, na bazi bakterije iz roda Pseudomonas, za suzbijanje uzročnika bijele noge (Rhizoctonia solani) na krumpiru, batatu, čičoki i kineskom jamu te uzročnika srebrolikosti gomolja (Helminthosporium solani) na krumpiru. Vrste Bacillus thuringiensis, Bacillus subtilis i Pseudomonas fluorescens su najvažniji predstavnici bakterija uključenih u biokontrolu protiv uzročnika biljnih bolesti. Do danas je na svjetskom tržištu prisutno ili je u procesu registracije 28 sredstava na bazi različitih vrsta roda Trichoderma, koji većinom djeluju protiv fitopatogenih gljiva iz rodova Pythium, Rhizoctonia i Fusarium. Najzastupljenije vrste roda Trichoderma koje se koriste u biološkoj kontroli su Trichoderma harzianum, Trichoderma viride i Trichoderma koningii. Upotreba antagonističkih gljiva i bakterija u biološkoj kontroli brojnih uzročnika bolesti predstavlja važnu alternativu kojom bi se mogli zamijeniti kemijski pesticidi ili u što većoj mjeri smanjiti njihova primjena. Cilj rada je prikazati antagonističke gljive i bakterije koje se koriste u biološkoj kontroli te opisati njihove složene mehanizme djelovanja.

scholarly journals Raw Secondary Metabolites of Chitosan-enriched Pseudomonas fluorescens P60 to Control Corn Sheath Blight

2021 ◽  
Vol 13 (1) ◽  
pp. 113-120
Author(s):  
Loekas Soesanto ◽  
Clarissa Nadya Pradiptha ◽  
Endang Mugiastuti
Keyword(s):  
Secondary Metabolites ◽  
Pseudomonas Fluorescens ◽  
Incubation Period ◽  
Infection Rate ◽  
Plant Protection ◽  
Block Design ◽  
Sheath Blight ◽  
Plant Diseases ◽  
In Vitro Test ◽  
Fresh Weight

Corn is a strategic cereal with economic value and always face the corn sheath blight in the field. An alternative safely and environmentally friendly disease control is the use of biological agent Pseudomonas fluorescens P60 that produced raw secondary metabolites. This study aimed to test the effectiveness of chitosan enriched P. fluorescens P60 raw secondary metabolites against leaf blight and to determine the effect on the growth of maize. The research was conducted at the Laboratory of Plant Protection and Experimental Farm, Faculty of Agriculture, Jenderal Soedirman University. In vitro test used a completely randomized design and in planta test used a randomized block design consisted of four treatments and six replicates. The treatments consisted of control and the secondary metabolites of P. fluorescens P60 enriched with chitosan 1, 2, and 3%. Variables observed were inhibition ability, chitinase and protease analysis qualitatively, incubation period, disease intensity, infection rate, AUDPC, crop height, crop fresh weight, and root fresh weight. The results showed that the secondary metabolites of P. fluorescens P60 enriched with chitosan 3% was effective in inhibiting the growth of Rhizoctonia solani as 63.3%, produced chitinase and protease, and could control the disease by delaying the incubation period as 79.05%, lowering the disease intensity as 68.68%, lowering the infection rate as 100%, and lowering AUDPC as 83.32%. The secondary metabolites of P. fluorescens P60 enriched with chitosan 3% was effective in improving plant growth by increasing plant fresh weight as 33.9% and root fresh weight as 43.09% compared to control. Chitosan could be used for improving effectiveness of antagonistic bacteria raw secondary metabolites. The raw secondary metabolites of bacterial antagonists could be improved their effectiveness against plant diseases by enrichment of chitosan.

scholarly journals Raw Secondary Metabolites Application of Two Trichoderma harzianum Isolates towards Vascular Streak Dieback on Cocoa Seedlings

2019 ◽  
Vol 35 (1) ◽  
pp. 22-32
Author(s):  
Loekas Soesanto ◽  
Endang Mugiastuti ◽  
Abdul Manan
Keyword(s):  
Secondary Metabolites ◽  
Plant Height ◽  
Incubation Period ◽  
Phenolic Compound ◽  
Trichoderma Harzianum ◽  
Block Design ◽  
Dry Weight ◽  
Fresh Weight ◽  
Number Of Leaves ◽  
Cocoa Seedlings

Vascular streak dieback is an important disease on cocoa that recently is still difficult to control. This research aimed to study the raw secondary metabo- lites application effectiveness of Trichoderma harzianum T10 and T213, or their combination to vascular streak dieback and its effect on growth and on phenolic compound of cocoa seedlings. Randomized block design was used with nine replicates. The treatments were control, secondary metabolites of T. harzianumT10 and T213, their combination and fungicide with active ingredient of phosphic acid. Variables observed were incubation period, disease intensity, plant height, number of leaves, wieght of fresh and dry plants, weight of fresh and dry roots, roots length, and phenolic compound. Result of the research showed that the raw secondary metabolites of T. harzianum T10 could suppress the disease inten- sity and delay the incubation period as 62.17 and 24.97%, respectively. The raw secondary metabolites of T. harzianum T213 and T10 combination could increase plant height 36.21%, number of leaves 19.07%, fresh weight of plant 20.6%, dry weight of plant 17.5%, fresh weight roots 27%, dry weight of root 34.36%, and roots length 21.30%. All raw secondary metabolites of T. harzianum were able to increase phenolic compounds (saponins, tannins, and glycosides) qualitatively of cocoa seedlings.

Growth of Listeria monocytogenes in the Presence of Pseudomonas fluorescens at 7 or 13°C in Skim Milk

1989 ◽  
Vol 52 (12) ◽  
pp. 852-855 ◽  
Author(s):  
SEHAM A. FARRAG ◽  
ELMER H. MARTH
Keyword(s):  
Listeria Monocytogenes ◽  
Pseudomonas Fluorescens ◽  
Incubation Period ◽  
Skim Milk

Autoclaved samples of skim milk were inoculated with Listeria monocytogenes (strain Scott A, California or V7), Pseudomonas fluorescens (strain P26 or B52), or a combination of L. monocytogenes plus P. fluorescens, and incubated at 7 or 13°C for 8 weeks. McBride Listeria Agar was used to determine populations of L. monocytogenes (at 0, 7, 14, 28, 42, or 56 d), and Pseudomonas isolation agar to enumerate P. fluorescens. Growth of L. monocytogenes was somewhat enhanced after 7 d of incubation at 7 but not at 13°C in the presence of pseudomonads. However, after 14 d and until the end of the incubation period (56 d), slight inactivation of L. monocytogenes in the presence of P. fluorescens was observed. L. monocytogenes did not affect growth or survival of P. fluorescens; also, no marked changes in pH of the milk were caused either by L. monocytogenes alone or by L. monocytogenes plus P. fluorescens.

scholarly journals Using maize wastes, fermented by co-cultures of Trichoderma harzianum and Pseudomonas fluorescens, as grain dressing against m maize diseases under field conditions

2020 ◽  
Vol 30 (1) ◽  
Author(s):  
Sherien M. M. Atalla ◽  
Mokhtar M. Abdel-Kader ◽  
Nadia G. El-Gamal ◽  
Nehal S. El-Mougy
Keyword(s):  
Pseudomonas Fluorescens ◽  
Trichoderma Harzianum ◽  
Raw Materials ◽  
Chitosan Nanoparticles ◽  
Field Conditions ◽  
Optimum Ratio ◽  
Transmission Electron ◽  
Environmentally Safe ◽  
The Mean ◽  
Uv Visible

Abstract Maize (Zea mays L.) is one of the most economic crops in Egypt. Production of amylase from the waste of maize is the most economic and cheap renewable and most abundant raw materials present in environment. Biosynthesis of Cu-chitosan nanoparticles for amylase production by co-culturing between Trichoderma harzianum and Pseudomonas fluorescens at different ratios compared to free conditions was the main purpose of this study. The optimum ratio 8:2, recorded between P. fluorescens: T. harzianum, was the most promising for production of amylase produce 22.47 and 28.60 U/ml for free and nano, respectively. The UV visible spectral analysis Cu-chitosan NPs was 220 nm, while the mean diameter, using transmission electron microscopy was 0.5 μm. Application of fermented maize wastes by co-cultivation of P. fluorescence and T. harzianum, as a grain dressing before sowing declared the reduction in both root and foliar diseases during the maize growing season, starting from germination up to 70 days of its vegetative growth under field conditions. A promising approach is the creation and use of environmentally safe products, whose protective effect is based on the induction of hydrolase inhibitors in plants.

scholarly journals PENGARUH GLOBALISASI KEPADATAN PENDUDUK DAN PERTUMBUHAN EKONOMI TERHADAP DEGRADASI LINGKUNGAN DI INDONESIA

2020 ◽  
Vol 2 (4) ◽  
Author(s):  
Safriwan Safriwan ◽  
Idris Idris
Keyword(s):  
Population Density ◽  
Environmental Degradation ◽  
Research Result ◽  
Short Term ◽  
Method Error ◽  
Negative Effect ◽  
Economic Institute ◽  
Carbon Project ◽  
Positive Effect

Abstract : The study describes the effects on globalization population density andeconomic growth on environmental degradation in Indonesia. This research uses a timeseries data from year 1971 - 2017, with method Error Correction Model (ECM). Datasources from Global Carbon Project, KOF Swiss Economic Institute, and WorldBank. Research result explain that (1) Globalization in long term has a insignificantpositive effect on environmental degradation in Indonesia, but short term globalizationhas a insignificant negative effect on environmental degradation in Indonesia (2)Population density in long term has a significant positive , and short term has ainsignificant positive effect on environmental degradation in Indonesia (3) Economicgrowth in long and short term has a significant positive effect on environmentaldegradation in Indonesia.Keywords : Environmental Degradation, Globalization Population Density AndEconomic Growth.

scholarly journals The Effect of Population Density and Intercropping with Tomato on the Growth and Yield of Winged Bean (Psophocarpus tetragonolobus)

2019 ◽  
Vol 6 (02) ◽  
pp. 81-88
Author(s):  
Gerson Hans Maure ◽  
Muhammad Achmad Chozin ◽  
Edi Santosa
Keyword(s):  
Population Density ◽  
Photosynthetic Activity ◽  
Block Design ◽  
Nutrient Content ◽  
Winged Bean ◽  
Growth And Yield ◽  
Psophocarpus Tetragonolobus ◽  
Randomized Block Design ◽  
High Nutrient ◽  
Almost All

Winged bean (Fabaceae) is a tropical crop that has high nutrient content, and almost all parts of the plant are edible. The research aimed to evaluate the growth and production of winged bean in an intercropping system with tomato. The study was conducted at the Cikabayan Experimental Field of Bogor Agricultural University, Bogor, Indonesia, in a randomized block design with three replications during the rainy season of December 2017 to June 2018. Winged beans were planted in high and low population densities and intercropped with three tomato genotypes, “Tora”, F70030081-12-16-3 and “Apel Belgia”. Monoculture winged bean was assigned as control. Tomatoes were planted between the rows of the winged beans in the intercropping treatment. The results showed that the growth of winged bean in monoculture and intercropping systems was not significantly different. The photosynthetic activity of winged bean at three to five weeks after planting remained high, indicating that the vegetative growth was optimal in both systems. Intercropping increased the winged bean number of pods per plant by 12.66-19.52% compared to monoculture, irrespective of population density. Therefore, winged bean could be considered as suitable to grow in intercropping systems.

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