With the development of quality education, Ordinary Differential Equation, as a basic course of mathematics major in colleges and universities, has been reformed, innovated and developed. Especially under the support of education informatization, micro class and flipped classroom are widely used in the teaching of Ordinary Differential Equation, which has a positive impact on improving its teaching quality and efficiency. This paper starts with the necessity of the application of flipped classroom teaching mode supported by micro course in the teaching of Ordinary Differential Equation, and explores the specific application strategy research in the course practice, aiming to provide reference for other professional courses teaching reform.