Effect of Thymus ciliatus oil-based disinfectant solutions against bio-films formed by Bacillus cereus strains isolated from pasteurized-milk processing lines in Algeria

2018 ◽  
Vol 8 (1) ◽  
pp. 01-12
Author(s):  
Amina Kalai ◽  
Fadila Malek ◽  
Leila Bousmaha-Marroki
Keyword(s):  
Essential Oil ◽  
Organic Acids ◽  
Bacillus Cereus ◽  
Biofilm Formation ◽  
Chemical Cleaning ◽  
Pasteurized Milk ◽  
Thyme Oil ◽  
Milk Processing ◽  
Planktonic Growth

Bacillus cereus is a foodborne pathogen that often persists in dairy environments and is associated with food poisoning and spoilage. This spore-forming bacterium has a high propensity to develop biofilms onto dairy processing equipment and resists to chemical cleaning and disinfecting. This study deals with the in vitro application of thyme oil-based sanitizer solutions against biofilms formed by B. cereus genotypes which persist in pasteurized-milk processing lines. The effect of Thymus ciliatus essential oil on B. cereus planktonic cells and biofilms was assessed. The oil was tested alone and in combination with organic acids or industrial cleaning agents, in order to improve the removal of B. cereus recurrent genotypes. Minimal inhibitory concentrations of planktonic growth (MICs), biofilm formation (MBIC) and biofilm eradication (MBEC) of oil and organic acids were evaluated by microdilution assays. Thyme oil was more effective than organic acids against B. cereus planktonic growth, biofilm formation and established bio-films. High values of MICs were obtained for the three organic acids tested (3.5-4.5%) in comparison with those of essential oil (0.082-0.088%). The combination of oil with other antimicrobials as acetic acid, NaOH or HNO3 improves their effectiveness against B. cereus biofilms. These oil-based sanitizer solutions allow complete B. cereus biofilm eradication and should be an attractive candidate for the control and removal of biofilms in the dairy envi-ronment.

Effect of thyme essential oil against Bacillus cereus planktonic growth and biofilm formation

2018 ◽  
Vol 102 (23) ◽  
pp. 10209-10218 ◽  
Author(s):  
Jiamu Kang ◽  
Liu Liu ◽  
Xiaoxia Wu ◽  
Yuyang Sun ◽  
Zifei Liu
Keyword(s):  
Essential Oil ◽  
Bacillus Cereus ◽  
Biofilm Formation ◽  
Planktonic Growth ◽  
Thyme Essential Oil

Rosemary and Tea Tree Essential Oils Exert Antibiofilm Activities In Vitro against Staphylococcus aureus and Escherichia coli

2020 ◽  
Vol 83 (7) ◽  
pp. 1261-1267
Author(s):  
TING LIU ◽  
JINGFAN WANG ◽  
XIAOMAN GONG ◽  
XIAOXIA WU ◽  
LIU LIU ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Essential Oil ◽  
Biofilm Formation ◽  
Gas Chromatography Mass Spectrometry ◽  
Tea Tree ◽  
E Coli ◽  
Microdilution Method ◽  
Broth Microdilution Method

ABSTRACT The purpose of the present study was to determine the bioactive compounds in rosemary essential oil (REO) and tea tree essential oil (TEO) and to investigate their antibacterial and antibiofilm activities against Staphylococcus aureus and Escherichia coli in vitro. The MIC and MBC assays were performed to assess the antibacterial activity of these two EOs against S. aureus and E. coli with the broth microdilution method. A crystal violet assay was used to ascertain the effects of EOs on the biofilm formation of the test strains, and a tetrazolium bromide (MTT) assay was used to measure the level of inactivation of mature biofilms by EOs. Gas chromatography–mass spectrometry revealed 15 compounds in REO and 27 compounds in TEO, representing 97.78 and 98.13% of the total EO, respectively. Eucalyptol and α-pinene were found in high concentrations in REO, and the two major compounds in TEO were 4-terpineol and terpinolene. The MICs of REO for the two S. aureus and E. coli test strains were both 0.5 mg/mL, and the MICs of TEO for the two strains were both 0.25 mg/mL. Therefore, these EOs can significantly inhibit the formation of biofilms and induced morphological biofilm changes, as verified by scanning electron microscopy. Both EOs had destructive effects on the mature biofilm of the two test strains. TEO was more inhibitory than REO for biofilm formation by the two test strains. HIGHLIGHTS

scholarly journals In vitro susceptibility of Brachyspira hyodysenteriae to organic acids and essential oil components

2016 ◽  
Vol 78 (2) ◽  
pp. 325-328 ◽  
Author(s):  
Lien VANDE MAELE ◽  
Marc HEYNDRICKX ◽  
Dominiek MAES ◽  
Nele DE PAUW ◽  
Maxime MAHU ◽  
...  
Keyword(s):  
Essential Oil ◽  
Organic Acids ◽  
Brachyspira Hyodysenteriae ◽  
Oil Components

scholarly journals Effects of oregano, carvacrol and thymol on Staphylococcus aureus and Staphylococcus epidermidis biofilms

2007 ◽  
Vol 56 (4) ◽  
pp. 519-523 ◽  
Author(s):  
Antonia Nostro ◽  
Andrea Sudano Roccaro ◽  
Giuseppe Bisignano ◽  
Andreana Marino ◽  
Maria A. Cannatelli ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Essential Oil ◽  
Staphylococcus Epidermidis ◽  
Biofilm Formation ◽  
Inhibitory Concentration ◽  
Oregano Essential Oil ◽  
Planktonic Growth ◽  
Subinhibitory Concentrations

The aim of this study was to evaluate the effect of oregano essential oil, carvacrol and thymol on biofilm-grown Staphylococcus aureus and Staphylococcus epidermidis strains, as well as the effects of the oils on biofilm formation. For most of the S. aureus (n=6) and S. epidermidis (n=6) strains tested, the biofilm inhibitory concentration (0.125–0.500 %, v/v, for oregano, and 0.031–0.125 %, v/v, for carvacrol and thymol) and biofilm eradication concentration (0.25–1.0 %, v/v, for oregano and 0.125–0.500 %, v/v, for carvacrol and thymol) values were twofold or fourfold greater than the concentration required to inhibit planktonic growth. Subinhibitory concentrations of the oils attenuated biofilm formation of S. aureus and S. epidermidis strains on polystyrene microtitre plates.

scholarly journals Identification and genetic diversity of Bacillus cereus strains isolated from a pasteurized milk processing line in Algeria

2012 ◽  
Vol 93 (1) ◽  
pp. 73-82 ◽  
Author(s):  
F. Malek ◽  
B. Moussa Boudjemaa ◽  
A. Aouar-Métri ◽  
M. Kihal
Keyword(s):  
Genetic Diversity ◽  
Bacillus Cereus ◽  
Pasteurized Milk ◽  
Milk Processing ◽  
Processing Line

scholarly journals Autoinducer 2 Affects Biofilm Formation by Bacillus cereus

2006 ◽  
Vol 72 (1) ◽  
pp. 937-941 ◽  
Author(s):  
Sandrine Auger ◽  
Evelyne Krin ◽  
Stéphane Aymerich ◽  
Michel Gohar
Keyword(s):  
Bacillus Cereus ◽  
Biofilm Formation ◽  
Inhibitory Effect ◽  
Strain Atcc ◽  
Photorhabdus Luminescens ◽  
Autoinducer 2 ◽  
Exogenous Addition

ABSTRACT Cell-free supernatants from growing Bacillus cereus strain ATCC 10987 induced luminescence in a Photorhabdus luminescens ΔluxS mutant, indicating the production of functional autoinducer 2 (AI-2). The exogenous addition of in vitro synthesized AI-2 had an inhibitory effect on biofilm formation by B. cereus and promoted release of the cells from a preformed biofilm.

scholarly journals Effects of Myrcia ovata Cambess. essential oil on planktonic growth of gastrointestinal microorganisms and biofilm formation of Enterococcus faecalis

2010 ◽  
Vol 41 (3) ◽  
pp. 621-627 ◽  
Author(s):  
Cinthya S. Cândido ◽  
Cadmo Silton A. Portella ◽  
Bruno J. Laranjeira ◽  
Sérgio S. da Silva ◽  
Angela M.C. Arriaga ◽  
...  
Keyword(s):  
Essential Oil ◽  
Enterococcus Faecalis ◽  
Biofilm Formation ◽  
Planktonic Growth
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