scholarly journals Molecular epidemiology of Cryptosporidium spp. in North Lebanon

2018 ◽  
Vol 12 (02.1) ◽  
pp. 34S ◽  
Author(s):  
Marwan Osman ◽  
Sadia Benamrouz ◽  
Karine Guyot ◽  
Dima El Safadi ◽  
Hassan Mallat ◽  
...  

Cryptosporidium spp. are enteroparasites with worldwide distribution that infect the gastrointestinal tract of several vertebrates including humans. Human to human, zoonotic, foodborne and waterborne are reported as the main transmission routes of this parasite. Cryptosporidium spp. have been recognized as the predominant cause of waterborne and foodborne outbreaks. However, the epidemiological situation of cryptosporidiosis is not well known in Lebanon, a developing country with a population often affected by intestinal parasitic infections. This study was devoted to determine the prevalence and the genetic diversity of Cryptosporidium spp. in symptomatic hospitalized patients and in two children populations with different socio-economic level in North-Lebanon, as well as the risk factors associated with cryptosporidiosis. Fecal samples obtained from these populations were examined microscopically by modified Ziehl-Neelsen staining as well as nested PCR were done for the detection of Cryptosporidium oocysts. Out of 163 symptomatic hospitalized patients and 249 children, Cryptosporidium was present in 11% and 10.4% respectively according to microscopy examination and/or molecular tests. The genotyping showed the predominance of Cryptosporidium hominis in both populations. Subgenotype analysis of the isolates at the gp60 locus identified three subtypes IdA19, IbA10G2 and IaA18R3 for C. hominis and two subtypes IIaA15G1R1 and IIaA15G2R1 for C. parvum. Moreover, cryptosporidiosis was correlated with having meals outside home and presence of gastrointestinal symptoms especially diarrhea (p <0.05). This work constitutes the first molecular epidemiology study outlining risk factors associated with cryptosporidiosis in Lebanon. These findings support a need of a control program to prevent the circulation of this parasite.

Author(s):  
Cintia Aparecida de Oliveira Flores ◽  
Rayane Lemos Farias

O objetivo deste estudo é revisar conforme literatura científica os principais fatores de risco associados à desnutrição em pacientes hospitalizados. O estudo é uma revisão simples da literatura a partir de busca nas bases de dados Lilacs, Scielo, Google Acadêmico, Google e Pubmed. A partir dos achados podemos verificar que os fatores de risco que mais se destacaram foram: a perda de peso recente e involuntária, a idade superior aos 60 anos, a patologia apresentada (câncer ou infecção grave), baixa ingesta calórica/proteica no período de internação, sintomas gastrointestinais (náuseas e diarreias) e um tempo maior de internação. O tratamento da desnutrição compõe um relevante desafio, onde um diagnóstico adequado é fundamental para que uma terapia nutricional seja iniciada o mais rápido possível e proporcione um tratamento dietoterápico eficaz.Descritores: Estado Nutricional, Fatores de Risco, Hospitalizados, Desnutrição. Risk factors associated with malnutrition in hospitalized patients: a literature reviewAbstract: The objective of this study is to review, according to scientific literature, the main risk factors associated with malnutrition in hospitalized patients. The study is a simple review of the literature based on searches in the Lilacs, Scielo, Google Scholar, Google and Pubmed databases. From the findings, we can see that the risk factors that stood out the most were: recent and involuntary weight loss, age over 60 years, the pathology presented (cancer or severe infection), low caloric/protein intake in the period of hospitalization, gastrointestinal symptoms (nausea and diarrhea) and a longer hospital stay, other less reported factors also appear in the study, such as admission diagnosis, poor appetite, apparent bone and low BMI. The treatment of malnutrition constitutes a relevant challenge, where an adequate diagnosis is essential so that a nutritional therapy can be started as soon as possible and provide an effective diet therapy treatment.Descriptors: Nutritional Status, Risk Factors, Hospitalized, Malnutrition. Factores de riesgo asociados a la desnutrición en pacientes hospitalizados: revisión de la literaturaResumen: El objetivo de este estudio es revisar, de acuerdo con la literatura científica, los principales factores de riesgo asociados a la desnutrición en pacientes hospitalizados. El estudio es una simple revisión de la literatura mediante la búsqueda en las bases de datos Lilacs, Scielo, Google Scholar, Google y Pubmed. De los hallazgos se puede apreciar que los factores de riesgo que más destacaron fueron: pérdida de peso reciente e involuntaria, edad mayor de 60 años, patología presentada (cáncer o infección severa), bajo aporte calórico/proteico en el período de hospitalización, síntomas gastrointestinales (náuseas y diarrea) y estancia hospitalaria más prolongada. El tratamiento de la desnutrición constituye un desafío relevante, donde un diagnóstico adecuado es fundamental para iniciar lo antes posible una terapia nutricional y brindar un tratamiento dietético efectivo.Descriptores: Estado Nutricional, Factores de Riesgo, Hospitalizados, Desnutrición.


Medicine ◽  
2020 ◽  
Vol 99 (45) ◽  
pp. e22971
Author(s):  
Shuai Shao ◽  
Zhiling Zhao ◽  
Feng Wang ◽  
Dandan Chang ◽  
Yong Liu ◽  
...  

2016 ◽  
Vol 9 (1) ◽  
Author(s):  
Dickson Cheruiyot Rop ◽  
Benson Omweri Nyanchongi ◽  
Johnson Nyangeri ◽  
Vincent Obino Orucho

2015 ◽  
Vol 30 ◽  
pp. 1856
Author(s):  
R. Masmoudi ◽  
F. Charfeddine ◽  
F. Smaoui ◽  
L. Aribi ◽  
J. Aloulou ◽  
...  

2021 ◽  
Vol 39 (1) ◽  
pp. 13
Author(s):  
Arifin Budiman Nugraha ◽  
Umi Cahyaningsih ◽  
Etih Sudarnika

Cryptosporidial infection is one of the most common causes of diarrhea in humans and livestock worldwide. This study was conducted to estimate the prevalence of Cryptosporidium infection and to identify potential risk factors associated with shedding of oocysts in Bogor. A total of 308 faecal samples were collected from 136 calves less than 6 months, 44 from those 6-12 months and 128 from those than 12 months. Data of factors potentially associated with the likelihood of Cryptosporidium spp. infection were recorded (i.e., enviromental status, size of herd, and herd management). Cryptosporidium spp. oocyst was identified by using modified acid fast (Ziehl Neelsen) staining technique and microscopically examined under 400x magnifition. Results showed that the prevalence of cryptosporidiosis in Bogor was 21.1% (CI 95%; 16.5%-25.6%). The highest prevalence was 29% (CI 95%; 26.8%-31.7%) in cattle aged less than 6 months. The oocysts abundance were around <5 oocysts per microscopy visual area. Data was analyzed using logistic regression models.  Statistical analysis showed that there were association between cryptosporidiosis and calves aged less than 6 months with an odds ratio (OR) of 2.7 (CI 95%; 1.5-5.2) times compared with cattle aged more than 12 months.


2018 ◽  
Vol 74 (1) ◽  
pp. 5904-2018
Author(s):  
MIROSŁAW RÓŻYCKI ◽  
JACEK SROKA ◽  
EWA CHMURZYŃSKA ◽  
EWA BILSKA–ZAJĄC ◽  
JOLANTA ZDYBEL ◽  
...  

The literature on human mussel-borne protozoan and helminthic infections is widely dispersed in epidemiological and parasitological journals. This review is focused on humans as hosts for protozoan, trematode and nematode parasites associated with consumption of mussels. These infections are caused mainly by protozoans transferred as cysts and oocysts or trematodes transferred as cercariae or metacercariae. The main scope of the article covers the following genera: Cryptosporidium, Giardia, Toxoplasma, microsporidia, and Fasciola. Foods regarded until recently as quite exotic are currently becoming increasingly available to consumers. To avoid certain parasitic infections, consumers need to know the risk factors associated with consumption of popular sea foods, such as mussels. The article contains information that may be useful to persons with compromised immune response....


2015 ◽  
Vol 2 (suppl_1) ◽  
Author(s):  
Francisco Fernandez ◽  
Alejandro Cane ◽  
Raul Isturiz ◽  
Daniel Curcio ◽  
Mauricio Peralta

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