scholarly journals Imported brucellosis and Q-fever coinfection in Croatia: a case report

2018 ◽  
Vol 12 (06) ◽  
pp. 499-503
Author(s):  
Ljiljana Peric ◽  
Dario Sabadi ◽  
Ilija Rubil ◽  
Maja Bogdan ◽  
Marija Guzvinec ◽  
...  
Keyword(s):  
Case Report ◽  
Q Fever ◽  
Brucella Melitensis ◽  
16S Rrna Gene Sequencing ◽  
Immunofluorescence Assay ◽  
Rrna Gene ◽  
Excessive Sweating ◽  
First Case ◽  
Acute Q Fever ◽  
Loss Of Appetite

The brucellosis and Q-fever coinfection is very rarely reported. To our knowledge, this is the first case report of concomitant brucellosis and Q-fever, most likely imported in Croatia. A 30-year-old male agricultural worker was hospitalized on 22 April 2017 after a ten days fever up to 40°C with chills, shivering, excessive sweating, general weakness, loss of appetite and headache. A month and a half prior to the hospitalization he lost 18 kg of body weight. Three weeks before hospitalization the patient returned from Kupres (Bosnia and Herzegovina) where he was working for the past year on a sheep farm and consumed unpasteurized dairy products of sheep origin. At admission, his condition was moderately severe due to pronounced dehydration. Routine laboratory tests showed slightly elevated erythrocyte sedimentation rate, anemia, thrombocytopenia and elevated liver transaminases. The chest X-ray showed an inhomogeneous infiltrate of the lower right lung. Three sets of blood culture were cultivated. After 48 hours incubation, bacterial growth was detected in aerobic bottles. Gram-stained smear revealed small, gram-negative coccobacilli. Specimens were subcultured on blood and chocolate agar plates. Using a Vitek GN identification card, the isolated organism was identified as Brucella melitensis. 16S rRNA gene sequencing of the isolate confirmed it as a Brucella sp. Rose-Bengal test was positive, while Wright agglutination test showed a significant increase in antibody titer from 80 to 640 in paired sera. Using indirect immunofluorescence assay (IFA), Coxiella burnetii phase II IgM/IgG titers were 50 and 1024, respectively indicating acute Q-fever. The patient was treated with doxycycline and rifampicin. So far, there has been no relapse or signs of chronic infection.

scholarly journals Rickettsia burneti and Brucella melitensis co-infection: a case report and literature review

2021 ◽  
Author(s):  
Jiangqin Song ◽  
Xiaolong Li ◽  
Xiaorong Hu ◽  
Yan Ding ◽  
Junyang Zhou ◽  
...  
Keyword(s):  
Causative Agent ◽  
Q Fever ◽  
Brucella Melitensis ◽  
Total Duration ◽  
Immunofluorescence Assay ◽  
Excessive Sweating ◽  
Case Presentation ◽  
Gram Negative ◽  
Missed Diagnosis ◽  
Loss Of Appetite

Abstract Background Rickettsia burneti is the causative agent of Q fever, Brucella melitensis is the causative agent of brucellosis, both of which are intracellular parasitic gram-negative bacteria. Rickettsia burneti and Brucella melitensis coinfection is fairly rarely reported in clinical. Early diagnosis and treatment are of great significance to the treatment and prognosis of brucellosis and Q fever. Case Presentation Here, we report a case of Rickettsia burneti and Brucella melitensis co-infection. The patient is a 49-year-old sheepherder, was hospitalized for left forearm trauma. Three days after admission, the patient’s fever up to 39.0°C with excessive sweating, weakness, loss of appetite and headache. Rickettsia burneti IgM was detected positive by indirect immunofluorescence assay (IFA). After 72 hours blood culture incubation, bacterial growth was detected in aerobic bottles, Gram-negative bacilli were found in culture medium smear, the colony was identified as Brucella melitensis by mass spectrometry. The patient accept therapy of doxycycline (100 mg bid, po) and rifampicin (600 mg qd, po) for a total duration of four weeks. After receiving treatment, the patient’s symptoms disappeared rapidly, there has been no relapse or signs of chronic infection.Conclusion For high-risk practitioners, Q fever and brucellosis may be present in one patient, we should routinely test for both pathogens through a variety of tests to prevent missed diagnosis.

scholarly journals Campylobacter insulaenigrae bacteremia with meningitis: a case report

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Moe Kyotani ◽  
Tsuneaki Kenzaka ◽  
Hozuka Akita ◽  
Soichi Arakawa
Keyword(s):  
Blood Culture ◽  
Marine Mammals ◽  
Chronic Subdural Hematoma ◽  
16S Rrna Gene Sequencing ◽  
Enhancement Effect ◽  
Rrna Gene ◽  
Clinical Report ◽  
Bacterial Strains ◽  
Fisher Syndrome ◽  
First Case

Abstract Background The bacterium Campylobacter insulaenigrae was first isolated from marine mammals of Scotland in 2004. Only one case of C. insulaenigrae infection in humans has been previously reported. Case presentation An 89-year-old Japanese man without dementia was admitted to our hospital, because he presented with a fever of 38 °C and weakness in right leg since 5 days. He had organized chronic subdural hematoma (CSH), and no history of pre-infection. At the time of admission, he had paralysis of the extraocular muscle, ataxia, and low manual muscle test score of the right side. He was suspected to have Miller Fisher syndrome; however, these symptoms improved without any treatment. On day 22 in the hospital, the patient presented a fever of 38.8 °C, left cranial nerve disorder, and hemiplegia. On day 25, the patient presented with signs of meningeal irritation; cerebrospinal fluid examination indicated an increase in the number of apocytes and a low glucose level. A contrast magnetic resonance imaging (MRI) scan of the patient’s head indicated a contrast enhancement effect in his right meninges. The blood culture showed presence of spirillums; 16S rRNA gene sequencing confirmed that the spirillums in the blood culture were Campylobacter insulaenigrae (C. insulaenigrae). We started treatment with meropenem for bacteremia and meningitis. When the symptoms improved, meropenem was replaced with ampicillin, based on the result of the drug sensitivity test. The treatment continued for 4 weeks. Conclusions We report the first case of meningitis caused by C. insulaenigrae bacteremia in humans, and the second clinical report of C. insulaenigrae infection in humans. The bacterial strains isolated from humans and marine mammals had different genotypes. This suggests that different genotypes could be responsible for differences in the hosts. Further case studies are needed to establish the reasons behind the difference in the manifestations of C. insulaenigrae infections reported so far.

scholarly journals First case of an invasive Bacteroides dorei infection detected in a patient with a mycotic aortic aneurysm—raising a rebellion of major indigenous bacteria in humans: a case report and review

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Takayuki Matsuoka ◽  
Takuya Shimizu ◽  
Tadanori Minagawa ◽  
Wakiko Hiranuma ◽  
Miki Takeda ◽  
...  
Keyword(s):  
Aortic Aneurysm ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Gene Sequencing ◽  
16S Rrna Gene Sequencing ◽  
Resected Specimen ◽  
Rrna Gene ◽  
Sequencing Analysis ◽  
First Case ◽  
Rrna Gene Sequencing

Abstract Background Bacteroides dorei is an anaerobic gram-negative bacterium first described in 2006. Because of the high similarity in mass spectra between B. dorei and Bacteroides vulgatus, discriminating between these species is arduous in clinical practice. In recent decades, 16S rRNA gene sequencing has been a complementary method for distinguishing taxonomically close bacteria, including B. dorei and B. vulgatus, at the genus and species levels. Consequently, B. dorei has been shown to contribute to some diseases, including type 1 autoimmune diabetes mellitus and atherosclerotic diseases. However, there are no reports on invasive infectious diseases caused by B. dorei. This report describes the first case of direct invasion and colonisation of human tissue by B. dorei, thus providing a warning regarding the previously proposed application of B. dorei as a live biotherapeutic for atherosclerotic diseases. Case presentation A 78-year-old Japanese man complained of intermittent chest/back pain and was diagnosed with a mycotic thoracic aortic aneurysm by enhanced computed tomography on admission. Despite strict blood pressure control and empirical antibiotic therapy, the patient’s condition worsened. To prevent aneurysmal rupture and eliminate infectious foci, the patient underwent surgical treatment. The resected specimen was subjected to tissue culture and 16S rRNA gene sequencing analysis to identify pathogenic bacteria. A few days after the surgery, culture and sequencing results revealed that the pathogen was B. dorei/B. vulgatus and B. dorei, respectively. The patient was successfully treated with appropriate antibacterial therapy and after improvement, was transferred to another hospital for rehabilitation on postoperative day 34. There was no recurrence of infection or aneurysm after the patient transfer. Conclusions This report describes the first case of invasive infectious disease caused by B. dorei, casting a shadow over its utilisation as a probiotic for atherosclerotic diseases.

INFANTILE BERIBERI ASSOCIATED WITH WERNICKE'S ENCEPHALOPATHY

PEDIATRICS ◽  
1958 ◽  
Vol 21 (3) ◽  
pp. 409-420
Author(s):  
Ruth Alice Davis ◽  
Abner Wolf
Keyword(s):  
Case Report ◽  
Abdominal Distension ◽  
Acute Illness ◽  
Wernicke’S Encephalopathy ◽  
Vitamin Supplementation ◽  
Wernicke's Encephalopathy ◽  
Excessive Sweating ◽  
Dietary History ◽  
First Case ◽  
Pathologic Findings

An infant, 5½ months of age, died after a brief acute illness characterized by irritability and somnolence leading terminally to coma, and by excessive sweating, abdominal distension, tachypnea and tachycardia. Acidosis and azotemia were marked. He was found at necropsy examination to have pathologic findings compatible with both beriberi and Wernicke's encephalopathy. Investigation of the dietary history and analysis of the formula fed the patient confirmed the suspicion of deficient intake of thiamine. This is the first case report in which the coexistence of these two pathologic conditions in a child proven to have had an inadequate diet, has been documented. It lends further support to the thesis that Wernicke's encephalopathy is caused by nutritional deficiency. The importance of vitamin supplementation of restricted diets used in the therapy of infantile eczema is emphasized.

scholarly journals Pharmacist’s Intervention Considering the Prognosis for a Terminal Cancer Patient: A Case Report

Pharmacy ◽  
2020 ◽  
Vol 8 (4) ◽  
pp. 212
Author(s):  
Masahiro Okada ◽  
Kazuko Okazaki ◽  
Keisuke Kimura ◽  
Hiroki Sugihara ◽  
Fumiyoshi Murakami ◽  
...  
Keyword(s):  
Case Report ◽  
Health Care Providers ◽  
Prognostic Index ◽  
Oral Intake ◽  
Sigmoid Colon Cancer ◽  
Care Providers ◽  
Abdominal Wall Metastasis ◽  
First Case ◽  
Prognostic Prediction ◽  
Loss Of Appetite

Prognostic prediction has been reported to affect the decision of doctors and non-physician health care providers such as nurses, social workers, pastors, and hospice volunteers on the selection of appropriate medical interventions. This was a case of a 65-year-old woman who presented with a poor oral intake. The patient had a history of sigmoid colon cancer with abdominal wall metastasis and peritoneal dissemination. On the day of admission, nausea, anorexia, and malaise were noted, requiring immediate intervention. The patient’s prognosis was predicted using the Palliative Prognostic Index. The pharmacist suggested the use of dexamethasone tablets in order to alleviate the patient’s symptoms. Indeed, the administration of dexamethasone alleviated the symptoms of nausea, loss of appetite, and malaise. To the best of our knowledge, this is the first case report to demonstrate that prognosis prediction is important not only for other medical staff but also for pharmacists when deciding the need to initiate a treatment and continue such treatment, and when providing pharmacist interventions.

Acute Q fever lobar pneumonia: a case report

2005 ◽  
Vol 51 (3) ◽  
pp. e89-e91 ◽  
Author(s):  
Maria Tsironi ◽  
Panagiotis Andriopoulos ◽  
Spiros Fokas ◽  
George Nikokiris ◽  
Marina Mantzourani ◽  
...  
Keyword(s):  
Case Report ◽  
Q Fever ◽  
Lobar Pneumonia ◽  
Acute Q Fever

scholarly journals First Case Report of Fatal Sepsis Due to Campylobacter upsaliensis

2014 ◽  
Vol 53 (2) ◽  
pp. 713-715 ◽  
Author(s):  
Itaru Nakamura ◽  
Nami Omori ◽  
Ayaka Umeda ◽  
Kiyofumi Ohkusu ◽  
Tetsuya Matsumoto
Keyword(s):  
Case Report ◽  
Rare Case ◽  
Pcr Amplification ◽  
Rrna Gene ◽  
Sequencing Analysis ◽  
Content Type ◽  
First Case ◽  
Campylobacter Upsaliensis ◽  
Fatal Sepsis ◽  
The 16S Rrna Gene

We encountered a rare case of severe fatal infection in a 70-year-old woman due toCampylobacter upsaliensis, identified by PCR amplification and sequencing analysis of the 16S rRNA gene using DNA extracted from the isolates. To our knowledge, fatal sepsis due to this organism has never been described to date.

Atypical Acute Q Fever: A Case Report

2017 ◽  
Vol 30 (1) ◽  
pp. 38-40
Author(s):  
Hatice Kose ◽  
Fatih Temocin ◽  
Tugba Sari
Keyword(s):  
Case Report ◽  
Q Fever ◽  
Acute Q Fever

scholarly journals Septic arthritis due to Roseomonas gilardii in an immunocompetent adolescent

2009 ◽  
Vol 58 (11) ◽  
pp. 1514-1516 ◽  
Author(s):  
Sergio Fanella ◽  
Daryl Schantz ◽  
James Karlowsky ◽  
Ethan Rubinstein
Keyword(s):  
Septic Arthritis ◽  
Gene Sequencing ◽  
16S Rrna Gene Sequencing ◽  
Sports Injury ◽  
Bordetella Bronchiseptica ◽  
Rrna Gene ◽  
First Case ◽  
Vitek 2 ◽  
Rrna Gene Sequencing ◽  
Slow Growing

The genus Roseomonas comprises groups of slow-growing, Gram-negative coccobacilli, which only infrequently cause infection in humans. When identified, they are associated with immunocompromised adults, often causing bacteraemia. Due to their rarity, members of this genus can be overlooked or misidentified using automated laboratory identification systems. We report on an immunocompetent adolescent patient who developed septic arthritis due to Roseomonas gilardii following surgery for a sports injury. The isolate was initially misidentified as Bordetella bronchiseptica using the Vitek 2 system, but confirmed as R. gilardii based on 16S rRNA gene sequencing. To the best of our knowledge, this is the first case of a healthy paediatric patient with septic arthritis due to R. gilardii.

Postoperative spondylodiscitis due to Kytococcus schroeteri in a diabetic woman

2010 ◽  
Vol 59 (1) ◽  
pp. 127-129 ◽  
Author(s):  
H. Jacquier ◽  
A. Allard ◽  
P. Richette ◽  
H. K. Ea ◽  
M. J. Sanson-Le Pors ◽  
...  
Keyword(s):  
Prosthetic Valve Endocarditis ◽  
16S Rrna Gene Sequencing ◽  
Diabetic Woman ◽  
Rrna Gene ◽  
Postoperative Spondylodiscitis ◽  
Osteoarticular Infections ◽  
First Case ◽  
Skin Flora ◽  
Rrna Gene Sequencing ◽  
Kytococcus Schroeteri

Kytococcus schroeteri, a Gram-positive coccus, is usually regarded as part of the human skin flora. It has been described in prosthetic valve endocarditis but never as being involved in osteoarticular infections. We report here the first case of a spondylodiscitis due to K. schroeteri identified by 16S rRNA gene sequencing.

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