scholarly journals Prevalence of Staphylococcus aureus Isolated from some Street Hot Beverages in Abidjan, Côte d’Ivoire

2021 ◽  
pp. 48-55
Author(s):  
Paul Yao Attien ◽  
Atobla Koua ◽  
Kamirou Chabi-Sika ◽  
Haziz Sina ◽  
Ibrahim Konate ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Cote D'ivoire ◽  
Biochemical Analysis ◽  
Côte D’Ivoire ◽  
Egg Yolk ◽  
Diffusion Method ◽  
Biochemical Tests ◽  
Cote D’Ivoire ◽  
Gram Staining ◽  
Côte D'ivoire

Introduction: Staphylococcus aureus is one of the main pathogens found in street food, including hot beverages. However, information about S. aureus isolated from street hot beverages from coffee carts is very limited in Abidjan, Côte d’Ivoire. Aims: We aimed to characterize phenotypically S. aureus isolated from street hot beverages sold in Abidjan. Methodology: A total of 400 samples of hot beverage were collected and analyzed. The identification was made through conventional microbial and biochemical analysis. Macroscopic identification on the Baird Parker agar supplement with egg yolk tellurite. Microscopic observation through Gram staining as well as biochemical tests such as catalase, DNase and coagulase were performed. To confirm staphylococcal strains, the MALDI-TOF mass spectrometry was used. After identification, the susceptibility of the staphylococcal isolates was evaluated using disc diffusion method. Results: Result showed that most of Staphylococcus aureus (18.4%) were isolated from tea. All the strains of Staphylococcus aureus isolated from street beverages were sensitive to cefoxitin and vancomycin. All the S. aureus isolated from milk were resistant to Erythromycin. Although beverages are consumed hot, the presence of S. aureus in ready-to-drink beverage transmitted infections to consumers. Conclusion: This drink need attention for the seller and the user to avoid some infections.

scholarly journals Microbiological and chemical hazards of commercial attieke (a fermented cassava product) produced in the south of Côte d’Ivoire

2019 ◽  
Vol 3 (3) ◽  
pp. 187-190
Author(s):  
Kouamé Kohi Alfred ◽  
Bouatenin Koffi Maizan Jean Paul ◽  
Djeni N’dede Théodore ◽  
Dje Koffi Marcellin
Keyword(s):  
Heavy Metal ◽  
Staphylococcus Aureus ◽  
Cote D'ivoire ◽  
Côte D’Ivoire ◽  
The South ◽  
Cote D’Ivoire ◽  
Côte D'ivoire ◽  
Chemical Hazard ◽  
Viable Bacteria ◽  
Bacillus Spores

Abstract Objectives Information on the distribution and presence of microbiology and chemical hazard of commercial attieke (a fermented cassava product) produced in the south of Côte d’Ivoire were evaluated. Materials and Methods Microbiological analyses and chemical were carried out, which included the total viable bacteria, Staphylococcus aureus, Salmonella and Bacillus spores, heavy metal (pb, Cd, Mn, Fe, Cu, and Cr). Results and Conclusions The results revealed that the viable bacteria counts ranged from in all of samples. Staphylococcus aureus counts in the samples were comprised between (1.8 ± 0.4)102 (Jacqueville localitie) and (4.3 ± 1.8)103 (Abidjan localitie). While that of Bacillus cereus was ranged from (1.1 ± 0.9)102 (Adzopé localitie) and (5.1 ± 3.2)102 (Abidjan localitie). Salmonella spp. was not found. The levels of Pb in all samples were between 2.2 ± 0.1 mg/kg (Grand-lahou localitie) and 4.5 mg/kg ± 0.15 (Abidjan localitie) while the Cd levels of the samples varied between 0.02 ± 0.1 mg/kg (Adzopé localitie) and 0.07± 0.1 mg/kg (Divo localitie). The levels of Cr in the samples were comprised between 0.1 ± 0.03 mg/kg (Adzopé localitie) and 0.95 ± 0.1 mg/kg (Abidjan localitie) while Fe (7.3 ± 1.5 mg/kg) and Cu (1.7 ± 0.2 mg/kg) levels were the highest, respectively in Sikensi and Grand-Lahou localities. Samples from Divo localitie contained high amounts of Mn (1.6 ± 0.1 mg/kg). The occurrence of some microbiological and chemical hazard that commercial attieke collected in Cote d’Ivoire may act as a reservoir of pathogenic micro-organisms and heavy metal for human.

scholarly journals First Case of Qnr B6 and Qnr B7 Genes in Enterobacteriaceae Producing Extended-Spectrum Beta-Lactamases in Abidjan, Côte D'ivoire

2019 ◽  
pp. 1-6
Author(s):  
Gadou Victoire ◽  
Toty Abalé Anatole ◽  
Konan Fernique ◽  
Tiékoura Konan Bertin ◽  
Ouattara Mohamed Baguy ◽  
...  
Keyword(s):  
Resistance Genes ◽  
Cote D'ivoire ◽  
Côte D’Ivoire ◽  
Fluoroquinolone Resistance ◽  
Diffusion Method ◽  
Pasteur Institute ◽  
Cote D’Ivoire ◽  
Extended Spectrum ◽  
Côte D'ivoire ◽  
First Case

Aims: The aim of this study was to characterize fluoroquinolone resistance genes in enterobacteriaceae that produce extended-spectrum β-lactamases, isolated in Abidjan. Place and Duration of Study: Pasteur Institute of Côte d'Ivoire and research unit on emerging tropical infectious diseases of Aix-Marseille University from January 2017 at July 2017. Methodology: The study included 90 enterobacteriaceae producing extended-spectrum β-lactamases isolated from biological products from various hospital services in Abidjan. These strains have been pre-identified and stored at the Center for Biological Resources (CeReB) of Pasteur Institute of Côte d'Ivoire. The identification of the strains was confirmed using the mass spectrometry MALDI-TOF (MS) and the antibiotic sensitivity test was performed using Müeller Hinton's agar diffusion method. The fluoroquinolone resistant genes were detected by conventional PCR and then, sequenced. Results: The strains studied were Escherichia coli (44), Klebsiella pneumoniae (31) and Enterobacter cloacae (15). High resistance rates to ceftriaxone (96.7%), cefotaxime (95.6%), aztreonam (95.6%) and cefoxitin (72.2%) were observed in all strains producing broad spectrum β-lactamases. The resistance rate to fluororquinolones represented by ciprofloxacin was 86.7%. The fluoroquinolone resistance genes detected were qnr A (3.3%) and qnr B (42.2%). Sequencing identified the qnr A1 (3.3%), qnr B1 (31.1%), qnr B6 (2.2%) and qnr B7 (1.1%) genes. Conclusion: This study made it possible to identify fluoroquinolone resistance genes in enterobacteriaceae producing β-lactamases which have an extended spectrum in Abidjan.

scholarly journals Chemical Composition and Antibacterial Activities of the Essential Oils from the Leaves of Premna angolensis and Premna quadrifolia, Two Lamiaceae from Côte d’Ivoire

2020 ◽  
Vol 5 (8) ◽  
pp. 681-686
Author(s):  
KOUASSI Kouamé Séraphin ◽  
KOUAME Bosson Antoine ◽  
MAMYRBÉKOVA-BÉKRO Janat Akhanovna ◽  
BEKRO YvesAlain ◽  
FOFIE N’guessan Bra Yvette
Keyword(s):  
Chemical Composition ◽  
Cote D'ivoire ◽  
Côte D’Ivoire ◽  
Antibacterial Activities ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Chemical Compositions ◽  
Cote D’Ivoire ◽  
Côte D'ivoire ◽  
Germacrene D

This study was conducted with the objective of contributing to the valorization of aromatic and medicinal plants of Côte d’Ivoire. The essential oil (EO) of the leaves of two species of Premna, P. angolensis and P. quadrifolia obtained through steam distillation process, were analized through gas chromatography coupled with mass spectroscopy (GC-MS) and their antimicrobial activities was evaluated by agar diffusion method. The yields of EO extracted from the leaves of P. angolensis and P. quadrifolia are respectively 0.10 ± 0.02% and 0.40 ± 0.02%. 99.43% of the total chemical composition of the EO of P. angolensis leaves, are composed mainly of hydrocarbon sesquiterpenes (71.90%) followed by oxygenates (14.16%) and monoterpenes (13.22%). The main compounds found are β-caryophyllene (33.07%) and Humulene (10.78%). The EO of P. quadrifofia leaves, 99.59% of the composition was identified. It contains hydrocarbon sesquiterpenes (89.15%), followed by oxygenates compounds (8.61%) and monoterpenes (1.83%). The major compounds are αbulnesene (23.33%), germacrene D (18.83%) and caryophyllene (18.06%). The antimicrobial tests have shown that EO of P. quadrifolia leaves has no activites on studied strains and P. angolensis oil has a mild activity on Staphylococcus epidermidis and Klebsielle pneumoneae and a weak sensibility on Staphylococcus aureus CIP 4.83 and Candida albicans ATCC 10231. This study highlights chemical composition of the EO from P. angolensis and P. quadrifolia leaves. It shows more similarities of the chemical compositions of these two species of Premna and the interesting antimicrobial activity potential of P. angolensis EO

scholarly journals Antimicrobial resistance and virulence associated genes in Campylobacter jejuni isolated from chicken in Côte d’Ivoire

2019 ◽  
Vol 13 (08) ◽  
pp. 671-677
Author(s):  
Bernadette G Goualié ◽  
Eric E Akpa ◽  
Solange E Kakou-N’Gazoa ◽  
Honoré G Ouattara ◽  
Sébastien L Niamke ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Campylobacter Jejuni ◽  
Cote D'ivoire ◽  
Côte D’Ivoire ◽  
Antimicrobial Agents ◽  
Diffusion Method ◽  
Cote D’Ivoire ◽  
Putative Gene ◽  
Côte D'ivoire ◽  
Microbiological Methods

Introduction: Campylobacter jejuni is one of the major causes of gastroenteritis worldwide of the last century. The aim of this study was to investigate the antibiotics profiles and the virulence gene in C. jejuni strains isolated from chicken in Côte d’Ivoire. Methodology: A total of 336 chicken ceaca samples recovered from market of two municipality of Abidjan were examined by conventional microbiological methods and molecular test using PCR. The antibiotic susceptibility tests of the isolates were determined by disk diffusion method. The presence of virulence genes was examined using simple PCR method. Results: Among of 336 samples, 168 (50%) were positives for C. jejuni. Among the C. jejuni isolates, 159 strains (94.64%) were resistant to one or more antimicrobial agents. The highest percentage of antimicrobial resistance was found for Nalidixic acid (85.33%), Tétracyclin (71.76%) and Ciprofloxacin (55.65%). Moreover, MDR including 3, 4, 5 and 6 antibiotics families was detected in 16.66% of isolates. On the other hand, detection of virulence putative gene shows presence of cadF in 100% of tested strains. In addition, cdtA, cdtB and cdtC genes were detected in 100%; 89.51% and 90.32% respectively of C. jejuniisolates. Conclusion: Because of the key role of broiler chicken in human campylobacteriosis infection, it will important in first time to monitoring using of antibiotics in chicken farms and in second time to verify presence of campylobactériosis in country.

Évaluation de la qualité de vie (QDV) des patients diabétiques de type 2 au CHU de Yopougon, Abidjan, Côte d’Ivoire

2019 ◽  
Vol 13 (4) ◽  
pp. 369-374
Author(s):  
A. Yao ◽  
A. Hué ◽  
J. Danho ◽  
P. Koffi-Dago ◽  
M. Sanogo ◽  
...  
Keyword(s):  
Cote D'ivoire ◽  
Qualité De Vie ◽  
Côte D’Ivoire ◽  
Cote D’Ivoire ◽  
Côte D'ivoire
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