scholarly journals Effect of inulin and fulvic acid on survival, growth, immune system, and WSSV prevalence in Litopenaeus vannamei

2017 ◽  
Vol 43 (5) ◽  
pp. 912-921
Author(s):  
Anayeli Gutiérrez-Dagnino ◽  
Antonio Luna-González ◽  
Jesús A. Fierro-Coronado ◽  
Píndaro Álvarez-Ruíz ◽  
María del Carmen Flores-Miranda ◽  
...  
Keyword(s):  
Viral Load ◽  
Immune System ◽  
Fulvic Acid ◽  
Litopenaeus Vannamei ◽  
High Viral Load ◽  
White Shrimp ◽  
Toll Receptor ◽  
Genetic Level ◽  
Biochemical Level ◽  
Immune Related Genes

The effect of inulin and fulvic acid, added in the feed, on growth, survival, WSSV prevalence, and immune system was studied in Litopenaeus vannamei. To the above, a bioassay, with treatments in triplicate, was performed to test different additive concentrations. WSSV analysis was done in organisms infected with a relatively high viral load using single and nested PCR. At the end of the bioassay, hemolymph was extracted and the immune system was studied in hemocytes at biochemical and genetic level (quantitative PCR). The final growth was similar in all treatments and survival was between 66,7% and 93,3%. WSSV prevalence decreased 13% as compared to control. The number of hemocytes, phenoloxidase activity, and superoxide anion concentration were similar in all treatments. Inulin and fulvic acid modulated the expression of transglutaminase, superoxide dismutase, and prophenoloxidase genes, but not the Toll receptor. Additives do not negatively affect growth of white shrimp and they protect them against WSSV when infected with a relatively high viral load. Additives did not affect the immune system effectors studied at biochemical level but they modulated the expression of some immune-related genes in L. vannamei.

Differential expression of immune‐related genes in Pacific white shrimp, Litopenaeus vannamei, previously reared in biofloc and challenged with Vibrio harveyi

2019 ◽  
Vol 50 (8) ◽  
pp. 2039-2046 ◽  
Author(s):  
Diana Aguilera‐Rivera ◽  
Gabriela Rodríguez‐Fuentes ◽  
Karla‐Susana Escalante‐Herrera ◽  
Edlin Guerra‐Castro ◽  
Alejandra Prieto‐Davó ◽  
...  
Keyword(s):  
Differential Expression ◽  
Litopenaeus Vannamei ◽  
Vibrio Harveyi ◽  
Pacific White Shrimp ◽  
White Shrimp ◽  
Immune Related Genes

scholarly journals Extracellular Polysaccharide from Porphyridium cruentum (purpureum) Stimulates the Non-Specific Immune Activities on White Shrimp (Litopenaeus vannamei)

2020 ◽  
Author(s):  
Yenny Risjani ◽  
Nurul Mutmainnah ◽  
Siti Narsito Wulan ◽  
Yunianta Yunianta
Keyword(s):  
Immune Response ◽  
Immune System ◽  
Litopenaeus Vannamei ◽  
Porphyridium Cruentum ◽  
White Shrimp ◽  
Treatment Result ◽  
Protective Agent ◽  
Immersion Method ◽  
Immune Parameters ◽  
Vibrio Infection

White shrimps are susceptible to outbreaks of vibriosis because they do not have any adaptive immune system, they only have a non-specific innate immune system. The administration of EPS from microalgae Porphyridium cruentum (synonym: P. purpureum) on shrimps Litopenaeus vannamei was investigated to determine the effect of this immunostimulant on their non specific immune response and to test if EPS can be used as a protective agent for shrimp related to Vibrio infection. EPS was given to shrimps by immersion method on day 1 and booster on day 8. Shrimp hemocytes were taken on day 1 (EPS administration), day 7 (no treatment), day 8 (EPS booster) and day 9 (Vibrio infection) and tested for their immune response on each treatment. Result shows an increase in values ​​of all immune parameters in line with the increasing EPS concentration, except the Differential Haemocyte Count (DHC). In detail, an increase was noted in total hemocytes (THC) value, Phagocytotic Activity (PA), Respiratory Burst (RB) in line as the EPS concentration increase. Although there is a decrease after the infection, the value obtained is not lower than the control value. These results indicate that EPS from Porphyrydium enhances immune parameters in shrimp rapidly and has the ability as an immunostimulant or an immunomodulator. It is a good modulator for the non specific immune cells of Pacific white shrimps, and it can be used as a preventive agent against Vibrio.

Effect of lignite fulvic acid on growth, antioxidant ability, and HSP70 of Pacific white shrimp, Litopenaeus vannamei

2018 ◽  
Vol 26 (6) ◽  
pp. 1519-1530 ◽  
Author(s):  
Yang Gao ◽  
Jingting Zhu ◽  
Huajiang Bao ◽  
Vector Hector ◽  
Bo Zhao ◽  
...  
Keyword(s):  
Fulvic Acid ◽  
Litopenaeus Vannamei ◽  
Pacific White Shrimp ◽  
White Shrimp ◽  
Antioxidant Ability

Effect of galangal (Alpinia galanga Linn.) extract on the expression of immune-related genes and Vibrio harveyi resistance in Pacific white shrimp (Litopenaeus vannamei)

2014 ◽  
Vol 23 (1) ◽  
pp. 385-399 ◽  
Author(s):  
Tidaporn Chaweepack ◽  
Surachart Chaweepack ◽  
Boonyee Muenthaisong ◽  
Lila Ruangpan ◽  
Kei Nagata ◽  
...  
Keyword(s):  
Litopenaeus Vannamei ◽  
Vibrio Harveyi ◽  
Pacific White Shrimp ◽  
White Shrimp ◽  
Alpinia Galanga ◽  
Immune Related Genes

scholarly journals Effect of microbial immunostimulants on WSSV infection percentage and the expression of immune-related genes in white shrimp (Litopenaeus vannamei)

2018 ◽  
Author(s):  
Jesús A Fierro Coronado ◽  
Antonio Luna González ◽  
Carlos J Caceres Martínez ◽  
Píndaro Álvarez Ruiz ◽  
Ruth Escamilla Montes ◽  
...  
Keyword(s):  
Litopenaeus Vannamei ◽  
White Shrimp ◽  
Immune Related Genes ◽  
Infection Percentage

scholarly journals Molecular effects of mannanase-hydrolyzed coprameal to intestinal immunity in the Pacific white shrimp (Litopenaeus vannamei)

2021 ◽  
Author(s):  
Wanilada Rungrassamee ◽  
Sopacha Arayamethakorn ◽  
Nitsara Karoonuthaisiri ◽  
Shih-Chu Chen ◽  
Eric Chang ◽  
...  
Keyword(s):  
Immune System ◽  
Litopenaeus Vannamei ◽  
Control Diet ◽  
Pacific White Shrimp ◽  
White Shrimp ◽  
Control Group ◽  
Intestinal Immunity ◽  
Toll Like Receptor ◽  
Transcript Levels ◽  
Significant Difference

AbstractTo mitigate disease outbreak, an alternative approach through enhancing shrimp immunity was explored. Mannan oligosaccharides (MOS) have been previously reported to enhance shrimp immune system. Here, coprameal samples were digested with mannanase to yield MOS, namely, mannanase-hydrolyzed coprameal (MCM) and feasibility of MCM as shrimp immunostimulant in grow-out ponds was determined. Pacific white shrimp (Litopenaeus vannamei) were fed with the commercial diet containing 1% MCM as the MCM-supplemented group and compared to the non-MCM control diet. There was no significant difference in survival rates between the MCM-supplemented and the control groups throughout the 4-month-period of the trial (p > 0.05). Gene expression analysis in shrimp intestines revealed that the transcript levels of antimicrobial peptides (anti-lipopolysaccharide factor isoform 1 (alf1), penaeidin (pen3a) and crustin (crus)) and lysozymes (lyz) were not significantly different in the MCM-supplemented group. Meanwhile, C-type lectin and toll-like receptor transcript levels, whose gene products play roles as pattern recognition proteins, were significantly higher in a group fed with MCM for 2- and 4-month periods than those of the control group (p < 0.05). The increased transcript levels of C-type lectin and toll-like receptor provide evidence for potential implementation of MCM as feed supplement to modulate shrimp immune system.

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