scholarly journals The Effect of Mesenchymal Stem Cell Wharton’s Jelly on Nuclear Factor Kappa Beta and Interleukin-10 Levels in Osteoarthritis Rat Model

2020 ◽  
Vol 8 (B) ◽  
pp. 350-357
Author(s):  
Vivi Sofia ◽  
Moch Saiful Bachri ◽  
Endrinaldi Endrinaldi
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Wharton’S Jelly ◽  
Joint Disease ◽  
Control Group ◽  
Nuclear Factor ◽  
Wharton's Jelly ◽  
Knee Oa ◽  
Nuclear Factor Kappa ◽  
Nuclear Factor Kappa Beta

BACKGROUND: Osteoarthritis (OA) is a degenerative joint disease in one or more joints characterized by changes in pathological structures such as cartilage, hypertrophy, and remodeling of the subchondral bone and secondary inflammation of the synovium membrane, causing changes in joint components such as cells, matrices, and molecular production. At the molecular level, an imbalance between catabolic and anabolic activities in joint cartilage results in OA. Nuclear factor kappa beta (NFκβ) is a cytokine that plays an important role in the signaling pathway of the pathogenesis of OA in causing an inflammatory reaction, whereas interleukin (IL)-10 is an anti-inflammatory cytokine that is involved in the pathogenesis of OA. AIM: This study aims to prove the influence before and after administration mesenchymal stem cells from Wharton’s jelly on the serum NFκβ and IL-10 levels in OA rat models. MATERIALS AND METHODS: This research is an experimental study with the design of post-test-only control group design. The sample consisted of 16 OA rats as a control group and 16 OA rats treated with MSC-WJ as a treatment group. OA induction is done by injection of monosodium iodoacetate (MIA) into the intra-articular right knee. Giving MSC-WJ is done in the 3rd week after MIA induction. The serum NFκβ and IL-10 levels were measured after 3 weeks treated with MSC-WJ using the ELISA method. The statistical test used is an independent t-test. p < 0.05 was said to be statistically significant. RESULTS: From the research results obtained, serum levels of knee OA of rat knee OA treated with mesenchymal stem cell Wharton jelly are lower than serum NFκβ levels of knee OA of the rat that is not treated, but the difference in levels of NFκβ is not significant (p > 0.05). The serum IL-10 level of rat OA of knee treated with mesenchymal stem cell Wharton jelly was higher than the serum IL-10 level of rat OA of the knee that was not treated, difference in levels of IL-10, is significant (p < 0.05). CONCLUSION: This study concluded that MSC-WJ significantly decreased the serum NFκβ levels of OA rats and not significantly increased the serum IL-10 levels of OA rats.

Branched chain amino acids improve mesenchymal stem cell proliferation, reducing nuclear factor kappa B expression and modulating some inflammatory properties

Nutrition ◽  
2020 ◽  
Vol 78 ◽  
pp. 110935
Author(s):  
Talita Sartori ◽  
Andressa Cristina Antunes Santos ◽  
Renaira Oliveira da Silva ◽  
Gabriela Kodja ◽  
Marcelo Macedo Rogero ◽  
...  
Keyword(s):  
Amino Acids ◽  
Cell Proliferation ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Nuclear Factor Kappa B ◽  
Branched Chain Amino Acids ◽  
Nuclear Factor ◽  
Stem Cell Proliferation ◽  
Nuclear Factor Kappa ◽  
Branched Chain

Mesenchymal stem cell separation from Wharton’s jelly and its differentiation into keratinocytes

2013 ◽  
Vol 22 (4) ◽  
pp. 547-553
Author(s):  
Irene Ellia M. Bishai ◽  
Mervat Saad El Ansary ◽  
Noha M. Hosny Shaheen ◽  
Reem Jan Farid
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Separation ◽  
Wharton’S Jelly ◽  
Wharton's Jelly

scholarly journals Borate reduces experimental supra-celiac aortic clamping-induced oxidative stress in lung and kidney, but fails to prevent organ damage

2021 ◽  
Vol 29 (3) ◽  
pp. 320-329
Author(s):  
Tünay Kurtoğlu ◽  
Selim Durmaz ◽  
Ömer Faruk Rahman ◽  
Nesibe Kahraman Çetin ◽  
Mustafa Yılmaz ◽  
...  
Keyword(s):  
Dimethyl Sulfoxide ◽  
Ischemia Reperfusion ◽  
Interleukin 1 ◽  
Control Group ◽  
Nuclear Factor ◽  
Necrosis Factor Alpha ◽  
Aortic Clamping ◽  
Nuclear Factor Kappa ◽  
Histological Damage ◽  
Nuclear Factor Kappa Beta

Background: This study aims to investigate the effects of 2-aminoethoxydiphenyl borate (2-APB) on aortic clamping-induced lung and kidney tissue oxidation, tissue inflammation, and histological damage in a rat model. Methods: A total of 28 adult female Wistar albino rats were randomly allocated to four equal groups: Control group, ischemia-reperfusion group, dimethyl sulfoxide group, and 2-APB group. Animals in the control group underwent median laparotomy. In the remaining groups, supra-celiac aorta was clamped for 45 min and, then, reperfusion was constituted for 60 min. The 2-APB (2 mg/kg) was administered before clamping. The remaining groups received saline (ischemia-reperfusion group) or dimethyl sulfoxide (dimethyl sulfoxide group). Kidney and lung tissue samples were harvested at the end of reperfusion. Results: Aortic occlusion caused increased tissue total oxidant status and reduced total antioxidant status and glutathione levels in the ischemia-reperfusion and dimethyl sulfoxide groups. Tissue interleukin-1 beta and tumor necrosis factor-alpha levels, nuclear factor kappa beta activation, and histological damage severity scores were also higher in these groups. The 2-APB treatment eliminated the increase in total oxidant status and the decrease in total antioxidant status and glutathione levels. It also caused a decrease in the interleukin-1 beta levels, although it did not significantly alter the tumor necrosis factor-alpha levels, nuclear factor kappa beta immunoreactivity, and histological damage scores. Conclusion: Borate exerted a beneficial antioxidant effect as evidenced by reduced oxidative stress; however, it did not inhibit nuclear factor kappa beta activation and prevent histological damage in supra-celiac aortic clamping-induced kidney and lung injury in rats.

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