Proteomics analysis of fetal growth restriction and taurine‑treated fetal growth restriction rat brain tissue by 2D�DIGE and MALDI‑TOF/TOF MS analysis

Objective of the study: Comprehensive assessment of risk factors, the implementation of which leads to FGR with early and late manifestation. To evaluate the results of the first prenatal screening: PAPP-A, B-hCG, made at 11-13 weeks. Materials and Methods: A retrospective study included 110 pregnant women. There were 48 pregnant women with early manifestation of fetal growth restriction, 62 pregnant women with late manifestation among them. Results of the study: The risk factors for the formation of the FGR are established. Statistically significant differences in the indicators between groups were not established in the analyses of structures of extragenital pathology. According to I prenatal screening, there were no statistical differences in levels (PAPP-A, b-hCG) in the early and late form of FGR.

Download Full-text

Sleep Apnea and Fetal Growth Restriction

Case Medical Research ◽

10.31525/ct1-nct04084990 ◽

2019 ◽

Author(s):

Keyword(s):

Sleep Apnea ◽

Fetal Growth ◽

Fetal Growth Restriction ◽

Growth Restriction

Download Full-text

Pathogenetic mechanisms and basic approaches to Doppler diagnostics of prenatal centralization of blood circulation in fetal growth restriction syndrome

Practical medicine ◽

10.32000/2072-1757-2020-2-21-27 ◽

2020 ◽

Vol 18 (2) ◽

pp. 21-27

Author(s):

Yu. A. STEPANOVA ◽

◽

V. I. SEMINA ◽

Keyword(s):

Fetal Growth ◽

Fetal Growth Restriction ◽

Blood Circulation ◽

Growth Restriction ◽

Pathogenetic Mechanisms ◽

Basic Approaches

Download Full-text

INTRAUTERINE GROWTH RETARDATION - A REVIEW ARTICLE

Journal of Medicine and Pharmacy ◽

10.34071/jmp.2018.6.25a ◽

2018 ◽

pp. 184-195

Author(s):

Minh Son Pham ◽

Vu Quoc Huy Nguyen ◽

Dinh Vinh Tran

Keyword(s):

Fetal Growth ◽

Gestational Age ◽

Small For Gestational Age ◽

Fetal Growth Restriction ◽

Growth Restriction ◽

Growth Potential ◽

National Guidelines ◽

Intrauterine Growth ◽

No Gold Standard

Small for gestational age (SGA) and fetal growth restriction (FGR) is difficult to define exactly. In this pregnancy condition, the fetus does not reach its biological growth potential as a consequence of impaired placental function, which may be because of a variety of factors. Fetuses with FGR are at risk for perinatal morbidity and mortality, and poor long-term health outcomes, such as impaired neurological and cognitive development, and cardiovascular and endocrine diseases in adulthood. At present no gold standard for the diagnosis of SGA/FGR exists. The first aim of this review is to: summarize areas of consensus and controversy between recently published national guidelines on small for gestational age or fetal growth restriction; highlight any recent evidence that should be incorporated into existing guidelines. Another aim to summary a number of interventions which are being developed or coming through to clinical trial in an attempt to improve fetal growth in placental insufficiency. Key words: fetal growth restriction (FGR), Small for gestational age (SGA)

Download Full-text

The secrets of placental mosaicism: clinical observation of the full form of confined placental trisomy 22 and literature review

10.21516/2413-1458-2020-19-1-35-40 ◽

2020 ◽

Author(s):

I.V. Komarova, A.A. Nikiforenko, A.V. Fedunyak

Keyword(s):

Prenatal Diagnosis ◽

Literature Review ◽

Fetal Growth ◽

Fetal Growth Restriction ◽

Clinical Observation ◽

Growth Restriction ◽

Favorable Outcome ◽

Full Form ◽

Placental Mosaicism ◽

In Pregnancy

Literature reports of placental mosaicism, including trisomy 22, were analyzed. The chance of correlation of placental aneuploidy with fetus aneuploidy, also the probability of complications in pregnancy and fetal growth restriction and postnatal patients growth in the cases of confined placental mosaicism, were demonstrated. The case of prenatal diagnosis of confined placental mosaicism of trisomy 22 with favorable outcome is presented. The necessity of cytogenic assay of amniocytes and fetal lymphocytes in the case of placental heteroploidy diagnosis was emphasized.

Download Full-text

Liquid Chromatography Analysis of Clozapine in Rat Brain Tissue, Using its Molecularly Imprinted Polymer after Administration of Toxic Dose of Drug and Lipid Emulsion Therapy

Current Pharmaceutical Analysis ◽

10.2174/1573412914666180111160741 ◽

2019 ◽

Vol 15 (3) ◽

pp. 251-257

Author(s):

Bahareh Sadat Yousefsani ◽

Seyed Ahmad Mohajeri ◽

Mohammad Moshiri ◽

Hossein Hosseinzadeh

Keyword(s):

Rat Brain ◽

Brain Tissue ◽

Molecularly Imprinted Polymer ◽

Lipid Emulsion ◽

Limit Of Detection ◽

Imprinted Polymer ◽

Toxic Dose ◽

Molecularly Imprinted ◽

The Brain ◽

Rat Brain Tissue

Background:Molecularly imprinted polymers (MIPs) are synthetic polymers that have a selective site for a given analyte, or a group of structurally related compounds, that make them ideal polymers to be used in separation processes.Objective:An optimized molecularly imprinted polymer was selected and applied for selective extraction and analysis of clozapine in rat brain tissue.Methods:A molecularly imprinted solid-phase extraction (MISPE) method was developed for preconcentration and cleanup of clozapine in rat brain samples before HPLC-UV analysis. The extraction and analytical process was calibrated in the range of 0.025-100 ppm. Clozapine recovery in this MISPE process was calculated between 99.40 and 102.96%. The limit of detection (LOD) and the limit of quantification (LOQ) of the assay were 0.003 and 0.025 ppm, respectively. Intra-day precision values for clozapine concentrations of 0.125 and 0.025 ppm were 5.30 and 3.55%, whereas inter-day precision values of these concentrations were 9.23 and 6.15%, respectively. In this study, the effect of lipid emulsion infusion in reducing the brain concentration of drug was also evaluated.Results:The data indicated that calibrated method was successfully applied for the analysis of clozapine in the real rat brain samples after administration of a toxic dose to animal. Finally, the efficacy of lipid emulsion therapy in reducing the brain tissue concentration of clozapine after toxic administration of drug was determined.Conclusion:The proposed MISPE method could be applied in the extraction and preconcentration before HPLC-UV analysis of clozapine in rat brain tissue.

Download Full-text