scholarly journals Combination of oncolytic adenovirus and luteolin exerts synergistic antitumor effects in colorectal cancer cells and a mouse model

2017 ◽  
Vol 16 (6) ◽  
pp. 9375-9382 ◽  
Author(s):  
Boduan Xiao ◽  
Yun Qin ◽  
Chang Ying ◽  
Buyun Ma ◽  
Binrong Wang ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Mouse Model ◽  
Cancer Cells ◽  
Oncolytic Adenovirus ◽  
Antitumor Effects ◽  
Colorectal Cancer Cells

An Oncolytic Adenovirus Expressing SNORD44 and GAS5 Exhibits Antitumor Effect in Colorectal Cancer Cells

2017 ◽  
Vol 28 (8) ◽  
pp. 690-700 ◽  
Author(s):  
Sujing Yuan ◽  
Yu Wu ◽  
Yigang Wang ◽  
Jianhua Chen ◽  
Liang Chu
Keyword(s):  
Colorectal Cancer ◽  
Cancer Cells ◽  
Antitumor Effect ◽  
Oncolytic Adenovirus ◽  
Colorectal Cancer Cells

scholarly journals Helicteric Acid, Oleanic Acid, and Betulinic Acid, Three Triterpenes fromHelicteres angustifoliaL., Inhibit Proliferation and Induce Apoptosis in HT-29 Colorectal Cancer Cells via Suppressing NF-κB and STAT3 Signaling

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Dan Su ◽  
Yu-qiao Gao ◽  
Wei-bo Dai ◽  
Ying Hu ◽  
Yan-fen Wu ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cancer Cells ◽  
Betulinic Acid ◽  
Biological Activities ◽  
Colorectal Tumor ◽  
Antitumor Effects ◽  
Chinese Medicinal Herb ◽  
Stat3 Signaling ◽  
Colorectal Cancer Cells ◽  
Ht 29

Colorectal cancer (CRC) is one of the most common malignancies and most frequent cause of cancer death worldwide. The activation of both NF-κB and STAT3 signaling and the crosstalk between them play an important role in colorectal tumor.Helicteres angustifoliaL. is a type of commonly used Chinese medicinal herb and possesses a wide variety of biological activities. In the present study, we investigate the effects of three triterpenes fromH. angustifolia(HT) such as helicteric acid (HA), oleanic acid (OA), and betulinic acid (BA), on inhibiting CRC progression. Our results showed that HT extracts could decrease proliferation and induce apoptosis in HT-29 colorectal cancer cells. Moreover, HT extracts could suppress LPS-triggered phosphorylation of IKK, IκB, and NF-κB, attenuate IL-6-induced phosphorylation of JAK2 and STAT3, and suppress the expression of c-Myc, cyclin-D1, and BCL-xL, the downstream gene targets of NF-κB and STAT3. Therefore, HT extracts showed potent therapeutic and antitumor effects on CRC via inhibiting NF-κB and STAT3 signaling.

scholarly journals 1,6-Bis[4-(4-amino-3-hydroxyphenoxy)phenyl] diamantane potentiates in vitro and in vivo antitumor effects of irinotecan on human colorectal cancer cells

2016 ◽  
Vol 11 (5) ◽  
pp. 3551-3557
Author(s):  
PO-SHENG YANG ◽  
JANE-JEN WANG ◽  
YEA-HWEY WANG ◽  
WOAN-CHING JAN ◽  
SHIH-PING CHENG ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cancer Cells ◽  
Human Colorectal Cancer ◽  
Antitumor Effects ◽  
Colorectal Cancer Cells ◽  
Human Colorectal Cancer Cells

scholarly journals IL-24-armed oncolytic vaccinia virus exerts potent antitumor effects via multiple pathways in colorectal cancer

2020 ◽  
Author(s):  
Lili Deng ◽  
Xue Yang ◽  
Jun Fan ◽  
Yuedi Ding ◽  
Ying Peng ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Vaccinia Virus ◽  
Cancer Cells ◽  
Treatment Options ◽  
Antitumor Effects ◽  
Colorectal Cancer Cells ◽  
Cancer Tumor ◽  
Induced Apoptosis

Colorectal cancer is an aggressive malignancy for which there are limited treatment options. Oncolytic vaccinia virus isbeing developed as a novel strategy for cancer therapy. Arming vaccinia virus with immunostimulatory cytokines can enhance the tumor cell-specific replication and antitumor efficacy. Interleukin-24 (IL-24) is an important immune mediator, as well as a broad-spectrum tumor suppressor. Here, we constructed a targeted vaccinia virus of Guang9 strain harbored IL-24 (VG9-IL-24) to evaluate its antitumor effects. In vitro, VG9-IL-24 induced increased number of apoptotic cells and blocked colorectal cancer cells in the G2/M phase of the cell cycle. VG9-IL-24 induced apoptosis in colorectal cancer cells via multiple apoptotic signaling pathways. In vivo,VG9-IL-24 significantly inhibited the tumor growth and prolonged the survival both in human and murine colorectal cancer models. Besides, VG9-IL-24 stimulated multiple antitumor immune responses and direct bystander antitumor activity. Our results indicate that VG9-IL-24 can inhibit the growth of colorectal cancer tumor by inducing oncolysis and apoptosis as well as stimulating the anti-tumor immune effects. These findings indicate that VG9-IL-24 may exert a potential therapeutic strategy for combating colorectal cancer

scholarly journals Synergistic Suppression Effect on Tumor Growth of Colorectal Cancer by Combining Radiotherapy With a TRAIL-Armed Oncolytic Adenovirus

2019 ◽  
Vol 18 ◽  
pp. 153303381985329 ◽  
Author(s):  
Hangxiang Gao ◽  
Xin Zhang ◽  
Ying Ding ◽  
Rong Qiu ◽  
Yupeng Hong ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Tumor Necrosis Factor ◽  
Antitumor Activity ◽  
Cancer Cells ◽  
Tumor Necrosis ◽  
Xenograft Model ◽  
Oncolytic Adenovirus ◽  
Apoptotic Pathway ◽  
Colorectal Cancer Cells ◽  
Necrosis Factor

The combination of gene therapy and radiation is a promising new treatment for cancer. This study aimed to clarify the synergistic effect of targeted oncolytic adenovirus (radiotherapy-tumor necrosis factor-related apoptosis-inducing ligand) and radiotherapy on colorectal cancer cells and elucidate the mechanisms of the underlying antitumor activity. Viability, cell cycle status, and apoptosis of treated colorectal cancer cells were determined via MTT and flow cytometric assays. The molecular mechanism underlying apoptotic pathway activation was elucidated through Western blot analysis of caspase-8, caspase-3, and PARP proteins. Combination treatment with radiotherapy-tumor necrosis factor-related apoptosis-inducing ligand and radiotherapy displayed significantly greater antitumor activity than either of the monotherapies. The primary mechanism behind the antitumor activity in the SW480 and Lovo colorectal cancer cell lines was apoptosis induction through the caspase pathway and G1 phase arrest. In an SW480 xenograft model of colorectal cancer, the combination therapy achieved a significantly greater reduction in tumor volume than the monotherapies. Overall, in this study, we demonstrate that the oncolytic radiotherapy-tumor necrosis factor-related apoptosis-inducing ligand construct can sensitize human colorectal cancer cells to radiation-induced apoptosis both in vitro and in vivo. Therefore, our findings point toward a novel synergistic approach to colorectal cancer treatment.

scholarly journals Boswellic acid exerts antitumor effects in colorectal cancer cells by modulating expression of the let-7 and miR-200 microRNA family

2012 ◽  
Vol 33 (12) ◽  
pp. 2441-2449 ◽  
Author(s):  
M. Takahashi ◽  
B. Sung ◽  
Y. Shen ◽  
K. Hur ◽  
A. Link ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cancer Cells ◽  
Boswellic Acid ◽  
Antitumor Effects ◽  
Microrna Family ◽  
Colorectal Cancer Cells

scholarly journals α-Linolenic and γ-linolenic acids exercise differential antitumor effects on HT-29 human colorectal cancer cells

2020 ◽  
Vol 9 (4) ◽  
pp. 474-483 ◽  
Author(s):  
María José González-Fernández ◽  
Ignacio Ortea ◽  
José Luis Guil-Guerrero
Keyword(s):  
Colorectal Cancer ◽  
Cancer Cells ◽  
Linolenic Acid ◽  
Caspase 3 ◽  
Colorectal Cancer Cell Line ◽  
Human Colorectal Cancer ◽  
Antitumor Effects ◽  
Colorectal Cancer Cells ◽  
Microscopy Images ◽  
Ht 29

Abstract α-Linolenic acid (ALA, 18:3n-3) and γ-gamma linolenic acid (GLA, 18:3n-6) are polyunsaturated fatty acids (PUFA) that improve the human health. The present study focused on testing the in vitro antitumor actions of pure ALA and GLA on the HT-29 human colorectal cancer cell line. Cell viability was checked by MTT ((3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) test, cell membrane damage by the lactate dehydrogenase assay, apoptosis was tested by both caspase-3 activity trial and transmission electron microscopy images, and protein composition was analyzed by quantitative proteomics analysis. MTT test revealed IC50 values of 230 and 255 μM for ALA and GLA, respectively, at 72 h. After 24 h of incubation, both ALA and GLA induced apoptosis on HT-29 colorectal cancer cells according to the caspase-3 assay and microscopy images. SWATH/MS analysis evidenced that ALA significantly affected the mitochondrial protein import pathway and the citric acid cycle pathway, while GLA did not significantly affect any particular pathway. In summary, both ALA and GLA showed concentration-dependent inhibitory effects on HT-29 cells viability and induced cell death by apoptosis. ALA significantly affected cellular pathways, while GLA does not have specific actions on either pathway. Both n-3 and n-6 C18 PUFA are bioactive food components useful in the colorectal cancer prevention.

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