Comprehensive analysis of gene expression profiles provides insight into the pathogenesis of Crohn's disease

Ulcerative colitis (UC) and Crohn’s disease (CD) are characterized by an imbalance between pro-inflammatory and anti-inflammatory cytokines, interfering with the resolution of inflammation. Due to the crucial role of cytokines, new insights into their profiles in UC and CD would help to improve our understanding of pathogenesis and enable the development of new treatment modalities. We provide an expression profile of cytokines in UC and CD, using bioinformatics approach, and experimental validation of expression of the selected genes. We retrieved data and analyzed the cytokine gene expression profiles of UC and CD. From ten genes with inverse expression, common to CD and UC, BMP8B, LEFTY1 and INSL5 were selected for gene expression experimental validation. Experimentally, BMP8B and INSL5 were down-regulated in both CD and UC but followed the bioinformatics trend. The expression of genes LEFTY1 and BMP8B was statistically significant when comparing UC and CD in colon and the expression of gene LEFTY1 showed statistical significance when CD in ileum and colon were compared. Using the bioinformatics approach and experimental validation, we found differences in expression profiles between UC and CD for INSL5, LEFTY1 and BMP8B. These three promising candidate genes need to be further explored at different levels, such as DNA methylation and protein expression, to provide more evidence on their potential diagnostic role in CD and UC.

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Su1763 Distinctive Gene Expression Profiles in the Whole Blood of Patients With Moderate to Severe Ulcerative Colitis and Crohn's Disease

Gastroenterology ◽

10.1016/s0016-5085(13)61740-0 ◽

2013 ◽

Vol 144 (5) ◽

pp. S-470

Author(s):

Shannon Telesco ◽

Katherine Li ◽

Colleen W. Marano ◽

Christopher Gasink ◽

Keying Ma ◽

...

Keyword(s):

Gene Expression ◽

Ulcerative Colitis ◽

Crohn’S Disease ◽

Crohn's Disease ◽

Whole Blood ◽

Expression Profiles ◽

Gene Expression Profiles ◽

Severe Ulcerative Colitis

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P104 Gene expression profiles in inflamed colonic biopsies from newly diagnosed Crohn’s disease and ulcerative colitis patients differ depending on the age at diagnosis

Journal of Crohn s and Colitis ◽

10.1093/ecco-jcc/jjx180.231 ◽

2018 ◽

Vol 12 (supplement_1) ◽

pp. S146-S146

Author(s):

S Verstockt ◽

B Verstockt ◽

J Van Bouwel ◽

W Meert ◽

A Cortes Calabuig ◽

...

Keyword(s):

Gene Expression ◽

Ulcerative Colitis ◽

Crohn’S Disease ◽

Crohn's Disease ◽

Expression Profiles ◽

Gene Expression Profiles ◽

Age At Diagnosis ◽

Newly Diagnosed ◽

Colonic Biopsies

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Tu1926 Mucosal Gene Expression Profiles in Pouchitis and in Active Ileal Crohn's Disease are Very Similar

Gastroenterology ◽

10.1016/s0016-5085(12)63413-1 ◽

2012 ◽

Vol 142 (5) ◽

pp. S-879

Author(s):

Ingrid Arijs ◽

Jan Van der Goten ◽

Marc Ferrante ◽

Kathleen Machiels ◽

Vicky De Preter ◽

...

Keyword(s):

Gene Expression ◽

Crohn’S Disease ◽

Crohn's Disease ◽

Expression Profiles ◽

Gene Expression Profiles

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Effect of Herb-Partitioned Moxibustion on Autophagy and Immune-Associated Gene Expression Profiles in a Rat Model of Crohn’s Disease

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2019/3405146 ◽

2019 ◽

Vol 2019 ◽

pp. 1-13 ◽

Cited By ~ 1

Author(s):

Ji-meng Zhao ◽

Ya-nan Liu ◽

Han-dan Zheng ◽

Yan Huang ◽

Qin Qi ◽

...

Keyword(s):

Gene Expression ◽

Crohn’S Disease ◽

Crohn's Disease ◽

Signaling Pathways ◽

Normal Saline ◽

Expression Profiles ◽

Gene Expression Profiles ◽

Once Daily ◽

Expression Levels ◽

Infection Pathway

Objective. To investigate the immune regulation mechanism of herb-partitioned moxibustion in rats with Crohn’s disease (CD) focusing on autophagy. Methods. Rats were randomly divided into normal (N) group, CD model (M) group, CD model with herb-partitioned moxibustion (MM) group, normal with herb-partitioned moxibustion (NM) group, CD model with mesalazine (western medicine, Med ) group, and normal saline (NS) group, with 10 rats in each group. The CD model rats were prepared by trinitrobenzene sulphonic expect for the N group and NM group. After the CD rats model were established, the rats in the MM and NM groups were treated with herb-partitioned moxibustion at Tianshu (ST25) and Qihai (CV6) acupoints once daily for 7 days, and rats in the Med and NS groups were respectively treated with mesalazine enteric coated tablet and normal saline once daily for 7 days. After intervention, hematoxylin-eosin staining was used to observe the histological changes of colon; RNA sequencing was used to observe the changes in autophagy- and immune-associated gene expression profiles. In addition, autophagy- and immune-associated cytokines and signaling pathways in CD rats were also screened. Results. HPM significantly increased the body weight of CD rats (P<0.01) and improved the pathological injury of colon in CD rats (P<0.01). HPM also changed the expression of many autophagy- and immune-associated genes, especially downregulating the expression of autophagy-associated Nod2, Irgm genes as well as the receptor of immune-associated Il12b, Il22 (Il12rb1, Il22ra2) genes in the colon of CD rats. HPM also changed the enrichment levels of differentially expressed genes in the human T-cell leukemia virus type-1 infection pathway, the Epstein-Barr virus infection pathway, and the cell adhesion molecule pathway. In addition, the expression levels of Nod2, Irgm, IL-12b, and IL-22 mRNA were increased (all P< 0.01) in the M group compared to the N group, while the expression levels of Nod2, Irgm, IL-12b, and IL-22 mRNA were decreased (P<0.05 or P<0.01) in the MM and Med groups compared to the M group. Conclusion. Herb-partitioned moxibustion may effectively attenuate intestinal inflammation and promote the repair of colon mucosal injury of CD rats through the regulation of autophagy- and immune-associated gene expression and signaling pathways.

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