scholarly journals Gene expression profiling analysis reveals that the long non‑coding RNA uc.412 is involved in mesangial cell proliferation

2019 ◽  
Author(s):  
Minyi Yu ◽  
Zheng Guan ◽  
Shanwen Li ◽  
Xianli Wen ◽  
Huimin Shi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Proliferation ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Mesangial Cell ◽  
Non Coding Rna ◽  
Mesangial Cell Proliferation ◽  
Long Non Coding Rna ◽  
Profiling Analysis

Knock-Down of Long Non-Coding RNA ANRIL Suppresses Mouse Mesangial Cell Proliferation, Fibrosis, Inflammation via Regulating Wnt/β-Catenin and MEK/ERK Pathways in Diabetic Nephropathy

2020 ◽  
Author(s):  
Xun Fang ◽  
Jun Hu ◽  
Hongyan Zhou
Keyword(s):  
Cell Proliferation ◽  
Diabetic Nephropathy ◽  
Mesangial Cell ◽  
Mesangial Cells ◽  
Necrosis Factor Alpha ◽  
Monocyte Chemoattractant Protein 1 ◽  
Knock Down ◽  
Non Coding Rna ◽  
Mesangial Cell Proliferation ◽  
Long Non Coding Rna

Abstract Aims Our study aimed to investigate the role of long non-coding RNA ANRIL (lnc-ANRIL) knock-down in regulating cell activities, inflammation and downstream signaling pathways in mouse mesangial cellular diabetic nephropathy (DN) model. Methods The mouse mesangial cells (SV40-MES13 cells) were treated with high-glucose (HG) to construct cellular DN model. Lnc-ANRIL knock-down plasmid and control knock-down plasmid were transfected into HG-treated SV40-MES13 cells as Sh-ANRIL group and Sh-NC group respectively. Results Lnc-ANRIL expression was significantly higher in HG-treated SV40-MES13 cells compared with normal glucose-treated SV40-MES13 cells and osmotic control-treated SV40-MES13 cells. Lnc-ANRIL knock-down suppressed cell proliferation and promoted cell apoptosis in HG-treated SV40-MES13 cells. As for fibrosis, lnc-ANRIL knock-down reduced fibronectin and collagen I expressions in HG-treated SV40-MES13 cells. Besides, the expressions of supernatant tumor necrosis factor-alpha (TNF-α), monocyte chemoattractant protein-1 (MCP-1), interleukin (IL)-1β, IL-6, IL-8 and IL-18 were reduced in Sh-ANRIL group compared with Sh-NC group. Furthermore, Wnt3, β-catenin, p-MEK1 and p-ERK1 expressions were suppressed in Sh-ANRIL group compared with Sh-NC group, which suggested that lnc-ANRIL knock-down inhibited Wnt/β-catenin and MEK/ERK pathways in HG-treated SV40-MES13 cells. Conclusions Lnc-ANRIL knock-down suppresses mouse mesangial cell proliferation, fibrosis, inflammation, Wnt/β-catenin and MEK/ERK pathways in DN.

Su1055 - Gene Expression Profiling Identifies Ifng-As1/Nest as a Long Non-Coding RNA Associated with IBD

2018 ◽  
Vol 154 (6) ◽  
pp. S-469
Author(s):  
David M. Padua ◽  
Carl R. Rankin ◽  
Nicholas van Buuren ◽  
Karla Kirkegaard
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Non Coding Rna ◽  
Long Non Coding Rna

Gene Expression Profiling Identifies CDKN2B-AS1 as a Long Non-Coding RNA Associated with IBD and Regulated by TGF-Beta

2017 ◽  
Vol 152 (5) ◽  
pp. S144 ◽  
Author(s):  
Carl R. Rankin ◽  
Dimitrios Iliopoulos ◽  
Charalabos Pothoulakis ◽  
David M. Padua
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Tgf Beta ◽  
Non Coding Rna ◽  
Long Non Coding Rna

Preparation of Salmonella enterica Serovar Typhi Genomic DNA Microarrays for Gene Expression Profiling Analysis*

2009 ◽  
Vol 2009 (2) ◽  
pp. 206-212 ◽  
Author(s):  
Xiu-Mei SHENG ◽  
Xin-Xiang HUANG ◽  
Ling-Xiang MAO ◽  
Chao-Wang ZHU ◽  
Shun-Gao XU ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Salmonella Enterica ◽  
Genomic Dna ◽  
Dna Microarrays ◽  
Profiling Analysis

Gene expression profiling analysis reveals a crucial gene regulating metabolism in adventitious roots of neem (Azadirachta indica)

RSC Advances ◽  
2016 ◽  
Vol 6 (115) ◽  
pp. 114889-114898 ◽  
Author(s):  
Shanying Wang ◽  
Hao Zhang ◽  
Xinglin Li ◽  
Jian Zhang
Keyword(s):  
Gene Expression ◽  
Secondary Metabolites ◽  
Gene Expression Profiling ◽  
Azadirachta Indica ◽  
Expression Profiling ◽  
Adventitious Roots ◽  
Bioactive Secondary Metabolites ◽  
Profiling Analysis

Neem is a widely used traditional plant containing bioactive secondary metabolites, especially azadirachtin.

Global gene expression profiling in early-stage polycystic kidney disease in the Han:SPRD Cy rat identifies a role for RXR signaling

2011 ◽  
Vol 300 (1) ◽  
pp. F177-F188 ◽  
Author(s):  
Masanori Kugita ◽  
Kazuhiro Nishii ◽  
Miwa Morita ◽  
Daisuke Yoshihara ◽  
Hiroe Kowa-Sugiyama ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Proliferation ◽  
Kidney Disease ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Early Stage ◽  
Global Gene Expression ◽  
Rt Pcr ◽  
Polycystic Kidney ◽  
Global Gene Expression Profiling

Han:SPRD Cy is a spontaneous rat model of polycystic kidney disease (PKD) caused by a missense mutation in Pkdr1. Cystogenesis in this model is not clearly understood. In the current study, we performed global gene expression profiling in early-stage PKD cyst development in Cy/Cy kidneys and normal (+/+) kidneys at 3 and 7 days of postnatal age. Expression profiles were determined by microarray analysis, followed by validation with real-time RT-PCR. Genes were selected with over 1.5-fold expression changes compared with age-matched +/+ kidneys for canonical pathway analysis. We found nine pathways in common between 3- and 7-day Cy/Cy kidneys. Three significantly changed pathways were designated “Vitamin D Receptor (VDR)/Retinoid X Receptor (RXR) Activation,” “LPS/IL-1-Mediated Inhibition of RXR Function,” and “Liver X Receptor (LXR)/RXR Activation.” These results suggest that RXR-mediated signaling is significantly altered in developing kidneys with mutated Pkdr1. In gene ontology analysis, the functions of these RXR-related genes were found to be involved in regulating cell proliferation and organ morphogenesis. With real-time RT-PCR analysis, the upregulation of Ptx2, Alox15b, OSP, and PCNA, major markers of cell proliferation associated with the RXR pathway, were confirmed in 3- and 7-day Cy/Cy kidneys compared with 3-day +/+ kidneys. The increased RXR protein was observed in both the nucleus and cytoplasm of cystic epithelial cells in early-stage Cy/Cy kidneys, and the RXR-positive cells were strongly positive for PCNA staining. Taken together, cell proliferation and organ morphogenesis signals transduced by RXR-mediated pathways may have important roles for cystogenesis in early-stage PKD in this Pkdr1-mutated Cy rat.

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