scholarly journals Migration of mouse-induced pluripotent stem cells to glioma-conditioned medium is mediated by tumor-associated specific growth factors

2011 ◽  
Vol 2 (2) ◽  
pp. 283-288 ◽  
Author(s):  
SHINICHIRO KOIZUMI ◽  
CHUNYU GU ◽  
SHINJI AMANO ◽  
SEIJI YAMAMOTO ◽  
HAYATO IHARA ◽  
...  
Keyword(s):  
Stem Cells ◽  
Growth Factors ◽  
Induced Pluripotent Stem Cells ◽  
Conditioned Medium ◽  
Pluripotent Stem Cells ◽  
Specific Growth ◽  
Induced Pluripotent

scholarly journals Growth Factor-free Chondrogenic Differentiation from Induced Pluripotent Stem Cells using Minicircle Vectors

2018 ◽  
Author(s):  
Yeri Alice Rim ◽  
Yoojun Nam ◽  
Ji Hyeon Ju
Keyword(s):  
Stem Cells ◽  
Growth Factors ◽  
Growth Factor ◽  
Gene Delivery ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Bone Morphogenetic Protein 2 ◽  
Viral Gene ◽  
Induced Pluripotent ◽  
Viral Gene Delivery

The human degenerative cartilage has low regenerative potential. Chondrocyte transplantation offers a promising strategy for cartilage treatment and regeneration. Currently chondrogenesis using human pluripotent stem cells are accomplished using human recombinant growth factors. Here, we differentiated human induced pluripotent stem cells (hiPSCs) into chondrocytes and cartilage pellet using minicircle vectors. Minicircles are used as a non-viral gene delivery system for gene therapy in various diseases. Non-viral gene delivery can produce growth factors without integrating into the host genome. Minicircle vectors containing bone morphogenetic protein 2 (BMP2) and transforming growth factor, beta 3 (TGFβ3) were successfully generated and delivered to hiPSC-derived outgrowth (OG) cells. Cell pellets generated using minicircle-transfected OG cells successfully differentiated into chondrogenic lineage. Chondrogenic pellets transfected with growth factor-encoding minicircles effectively recovered osteochondral defect in rat models. Taken together, this work shows the potential application of minicircles in cartilage regeneration using hiPSCs.

Effects of induced pluripotent stem cells-derived conditioned medium on the proliferation and anti-apoptosis of human adipose-derived stem cells

2016 ◽  
Vol 413 (1-2) ◽  
pp. 69-85 ◽  
Author(s):  
Rui-Ling Lian ◽  
Xiao-Ling Guo ◽  
Jian-Su Chen ◽  
Yong-Long Guo ◽  
Jia-Fu Zheng ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Conditioned Medium ◽  
Pluripotent Stem Cells ◽  
Adipose Derived Stem Cells ◽  
Induced Pluripotent

Adipose‐derived stem cells‐conditioned medium improved osteogenic differentiation of induced pluripotent stem cells when grown on polycaprolactone nanofibers

2018 ◽  
Vol 234 (7) ◽  
pp. 10315-10323 ◽  
Author(s):  
Fatemeh Soleimanifar ◽  
Fatemeh Sadat Hosseini ◽  
Hadi Atabati ◽  
Asma Behdari ◽  
Ladan Kabiri ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Osteogenic Differentiation ◽  
Conditioned Medium ◽  
Pluripotent Stem Cells ◽  
Adipose Derived Stem Cells ◽  
Induced Pluripotent

Induced pluripotent stem cells’ self-renewal and pluripotency is maintained by a bovine granulosa cell line-conditioned medium

2011 ◽  
Vol 410 (2) ◽  
pp. 252-257 ◽  
Author(s):  
Claudia Solari ◽  
Noelia Losino ◽  
Carlos Luzzani ◽  
Ariel Waisman ◽  
Carolina Bluguermann ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Cell Line ◽  
Conditioned Medium ◽  
Granulosa Cell ◽  
Pluripotent Stem Cells ◽  
Self Renewal ◽  
Induced Pluripotent

The impact of growth factors on human induced pluripotent stem cells differentiation into cardiomyocytes

Life Sciences ◽  
2018 ◽  
Vol 196 ◽  
pp. 38-47 ◽  
Author(s):  
Marianne E. Yassa ◽  
Iman A. Mansour ◽  
Nadia I. Sewelam ◽  
Hala Hamza ◽  
Taghrid Gaafar
Keyword(s):  
Stem Cells ◽  
Growth Factors ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Induced Pluripotent ◽  
The Impact

scholarly journals A miniature dialysis-culture device allows high-density human-induced pluripotent stem cells expansion from growth factor accumulation

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Fuad Gandhi Torizal ◽  
Qiao You Lau ◽  
Masato Ibuki ◽  
Yoshikazu Kawai ◽  
Masato Horikawa ◽  
...  
Keyword(s):  
Stem Cells ◽  
Shear Stress ◽  
Growth Factors ◽  
Growth Factor ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
High Density ◽  
Induced Pluripotent ◽  
Dialysis Culture ◽  
Factor Accumulation

AbstractThree-dimensional aggregate-suspension culture is a potential biomanufacturing method to produce a large number of human induced pluripotent stem cells (hiPSCs); however, the use of expensive growth factors and method-induced mechanical stress potentially result in inefficient production costs and difficulties in preserving pluripotency, respectively. Here, we developed a simple, miniaturized, dual-compartment dialysis-culture device based on a conventional membrane-culture insert with deep well plates. The device improved cell expansion up to approximately ~3.2 to 4×107 cells/mL. The high-density expansion was supported by reduction of excessive shear stress and agglomeration mediated by the addition of the functional polymer FP003. The results revealed accumulation of several growth factors, including fibroblast growth factor 2 and insulin, along with endogenous Nodal, which acts as a substitute for depleted transforming growth factor-β1 in maintaining pluripotency. Because we used the same growth-factor formulation per volume in the upper culture compartment, the cost reduced in inverse proportional manner with the cell density. We showed that growth-factor-accumulation dynamics in a low-shear-stress environment successfully improved hiPSC proliferation, pluripotency, and differentiation potential. This miniaturised dialysis-culture system demonstrated the feasibility of cost-effective mass production of hiPSCs in high-density culture.

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