scholarly journals High expression of Janus kinase 2 in background normal liver tissue of resected hepatocellular carcinoma is associated with worse prognosis

2014 ◽  
Vol 33 (2) ◽  
pp. 767-773 ◽  
Author(s):  
FUMINORI SONOHARA ◽  
SHUJI NOMOTO ◽  
YOSHIKUNI INOKAWA ◽  
MITSUHIRO HISHIDA ◽  
NAO TAKANO ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Liver Tissue ◽  
Normal Liver ◽  
Janus Kinase ◽  
High Expression ◽  
Normal Liver Tissue ◽  
Janus Kinase 2 ◽  
Worse Prognosis

Elasticity Characterization of Liver Cancers Using Shear Wave Ultrasound Elastography: Comparison Between Hepatocellular Carcinoma and Liver Metastasis

2017 ◽  
Vol 33 (6) ◽  
pp. 481-488 ◽  
Author(s):  
Kah Lai Choong ◽  
Yin How Wong ◽  
Chai Hong Yeong ◽  
Gnana Kumar Gnanasuntharam ◽  
Khean Lee Goh ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Liver Metastasis ◽  
Shear Wave ◽  
Liver Tissue ◽  
Normal Liver ◽  
Ultrasound Elastography ◽  
Normal Liver Tissue ◽  
Tissue Elasticity ◽  
Liver Cancers

Purpose: This was a feasibility study of shear wave ultrasound elastography for characterization of liver tumors and to compare the tissue elasticity values of hepatocellular carcinoma (HCC), liver metastases, and normal liver tissues. Methods: Forty-one patients and 30 healthy volunteers were recruited and categorized into HCC, liver metastasis, and control groups based on their computed tomography and sonographic examinations. Elasticity values of different groups were compared statistically. Results: Mean (standard deviation) elasticity values for HCC, liver metastasis, and normal liver tissue were 51.45 (14.96), 49.89 (13.82), and 6.63 (1.65) kilopascal, respectively. Statistically significant differences were found between the elasticity values of HCC and liver metastasis with normal liver tissue. Based on the receiver operating characteristics analysis, 18.25 kilopascal may differentiate the malignant focal liver lesions from the normal liver tissue with both sensitivity and specificity of 100%. Conclusion: Shear wave ultrasound elastography may be able to differentiate HCC and liver metastasis from normal liver tissue based on the tissue elasticity values.

Distribution Characteristics and Species Diversity of Bacteria in Hepatocellular Carcinoma Tissues

2022 ◽  
Vol 12 (1) ◽  
pp. 97-104
Author(s):  
Yeqing Fang ◽  
Weili Zhang
Keyword(s):  
Hepatocellular Carcinoma ◽  
Species Diversity ◽  
Liver Tissue ◽  
Bacterial Species ◽  
Normal Liver ◽  
Normal Group ◽  
Normal Liver Tissue ◽  
Control Group ◽  
Sequencing Data ◽  
Liver Tissues

This study was to explore the differences in the distribution and species diversity of bacteria between hepatocellular carcinoma (HCC) tissues and normal liver tissues. 28 HCC patients treated with surgery were selected as the research objects (HCC group), and 19 healthy volunteers with normal physical examinations were included in the control group (Normal group). The tumor specimens were obtained by intraoperative and biopsy puncture, and a 16S ribosomal ribonucleic acid (rRNA) library was constructed. Based on the sequencing data obtained by the IlluminaHi Seq sequencing platform, the differences of bacteria in the liver tissues of the HCC group and the Normal group were analyzed at the level of phyla, family, and genus. The Ace, Chao1, and Shannon of the two groups were compared. The results showed that IlluminaHi Seq sequencing obtained a total of 11,714,659 valid sequences, with an average of 131,625 sequences per sample. The proportions of Bacteroidetes, Firmicutes, and Proteobacteria in HCC group and Normal group were 48.75% versus 34.16%, 37.44% versus 18.02%, and 10.85% versus 39.26%, respectively. The Bacteroidaceae, Prevotellaceae, Lachnospiraceae, and Ruminococcaceae accounted for 22.49%, 20.62%, 16.54%, and 19.93% in Normal group; while those in the HCC tissues accounted for 26.83%, 14.22%, 11.14%, and 13.18%, respectively. The dominant bacteria at the genus level in HCC group and Normal group were Bacteroides and Prevotella-9, with the proportions of 24.19% versus 26.04% and 14.19% versus 8.44%, respectively. The difference in operational taxonomic unit (OTU) numbers of HCC and Normal group were compared and analyzed, which were 1,266 and 1,082, respectively, and the number of common OTU in the two tissues was 875. The Ace in HCC tissue and normal liver tissue were 1063.8±66.79 and 1003.6±52.19, respectively. The Ace in HCC tissue was greater than that in normal liver tissue (P < 0.05). The Chao1 and Shannon in HCC tissue were 1022.9±67.74 and 5.4269±0.3608, respectively; while those in normal liver tissue were 1003.6±66.79 and 5.2842±0.9714, respectively. The Chao1 and Shannon in HCC tissues were much higher than those in Normal group (P < 0.05). It showed that there was no difference in the types of bacterial species in HCC tissues, but the proportions of their flora at the level of phyla, family, and genus changed greatly, which may be related to the occurrence of HCC. This study could provide a reference for the diagnosis and treatment of HCC.

scholarly journals Distinct hepatitis B virus integration patterns in hepatocellular carcinoma and adjacent normal liver tissue

2017 ◽  
Vol 140 (6) ◽  
pp. 1324-1330 ◽  
Author(s):  
Xiaobo Yang ◽  
Liangcai Wu ◽  
Jianzhen Lin ◽  
Anqiang Wang ◽  
Xueshuai Wan ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Liver Tissue ◽  
Normal Liver ◽  
Normal Liver Tissue ◽  
B Virus ◽  
Virus Integration

scholarly journals Effect of Intrahepatic Arterial Delivery of Sorafenib on Normal Liver Tissue of Rabbit: An Experimental Study

2020 ◽  
Vol 3 (1) ◽  
pp. 1
Author(s):  
Eerdunbagena Ning ◽  
Zhijun Wang
Keyword(s):  
Heart Rate ◽  
Rectal Temperature ◽  
Liver Tissue ◽  
High Performance ◽  
Arterial Embolization ◽  
Hepatic Arterial Infusion ◽  
Normal Liver ◽  
Normal Liver Tissue ◽  
Breathing Rate ◽  
Local Necrosis

Objective: To assess the safety, feasibility and eluting efficiency of intrahepatic arterial delivery of sorafenib on normal liver tissue of rabbit. Methods: 24 New Zealand rabbits were randomly divided into three groups: group Ⅰ (Lipiodol-sorafenid), group Ⅱ (Lipiodol) and group Ⅲ (Sorafenib). Group Ⅰ and Ⅱ were treated by transcatheter selective hepatic arterial embolization with emulsions of lipiodol and sorafenib or with only lipiodol, while group Ⅲ was given hepatic arterial infusion with sorafenib. Sorafenib concentration in plasma was determined by HPLC (high performance liquid chromatography) in 0 min, 20 min, 1h, 2h, 4h, 8h, 16h, 32h and 48h respectively. The breathing rate, heart rate, rectal temperature and body weight were measured, as well the blood routine test and the function of liver, kidney, and heart. Two animals of each group were respectively killed in the 3rd day, 1st, 3rd and 6th week after treatment. Histopathologic study was done to liver, heart, kinney, lung, brain, gall bladder and intestine. Result: ① The peak sorafenib concentration (Cmax)and AUC(Area under curve) in plasma in groupⅠwas 2.46±0.101μg/ml and 945.72 ± 52.3 μg/mL.min respectively, while in group Ⅲ which was 3.78±0.180 ug/ml and 546.98±21.1μg/mL.min. Compared with groupⅢ, the Cmax and AUC of groupⅠhad a significant statistics difference (p<0.05). ② The breathing rate, heart rate, rectal temperature and AST/ALT,WBC,NEU% of group Ⅰand groupⅢhas a significant statistics difference(p<0.05) in the 3rd day. ③CK ,CK-MB, DB, Cr，BUN,RBC,PLT in plasma did not change in all group.④Local necrosis was seen in group Ⅰand group Ⅱin the 3rd day and 1st week, but they did not seem to be different. Group Ⅲ showed no necrosis. Granulation tissue with bile duty, portal vein and microfossils hyperplasia were seen in local necrosis area in the 3rd week. No pathological changes were found in brain, heart, kidney, intestine and gallbladder. Conclusion: TAE with emulsions of lipiodol and sorafenib is feasible, safe and has some slow-release effect.

Normal liver tissue change after proton beam therapy

2018 ◽  
Vol 36 (9) ◽  
pp. 559-565 ◽  
Author(s):  
Nobuyoshi Fukumitsu ◽  
Shinsei Takahashi ◽  
Toshiyuki Okumura ◽  
Toshiki Ishida ◽  
Keiko Nemoto Murofushi ◽  
...  
Keyword(s):  
Proton Beam ◽  
Liver Tissue ◽  
Normal Liver ◽  
Proton Beam Therapy ◽  
Normal Liver Tissue ◽  
Tissue Change

Caffeine does not enhance radiosensitivity of normal liver tissue in vivo

2010 ◽  
Vol 38 (7) ◽  
pp. 4359-4367 ◽  
Author(s):  
Tie-Jun Wang ◽  
Zhong-Shan Liu ◽  
Zhao-Chong Zeng ◽  
Shi-Suo Du ◽  
Ming Qiang ◽  
...  
Keyword(s):  
Liver Tissue ◽  
Normal Liver ◽  
Normal Liver Tissue

Accuracy of real-time shear wave elastography in the assessment of normal liver tissue in the guinea pig (cavia porcellus)

2017 ◽  
Vol 20 (1) ◽  
pp. 51-56
Author(s):  
K. Glińska-Suchocka ◽  
K. Kubiak ◽  
J. Spużak ◽  
M. Jankowski ◽  
P. Borusewicz
Keyword(s):  
Guinea Pig ◽  
Real Time ◽  
Shear Wave ◽  
Liver Tissue ◽  
Ultrasound Image ◽  
Shear Wave Elastography ◽  
Normal Liver ◽  
Normal Liver Tissue ◽  
Invasive Technique ◽  
Non Invasive

Abstract Shear wave elastography is a novel technique enabling real-time measurement of the elasticity of liver tissue. The color map is superimposed on the classic ultrasound image of the assessed tissue, which enables a precise evaluation of the stiffness of the liver tissue. The aim of the study was to assess the stiffness of normal liver tissue in the guinea pig using shear wave elastography. The study was carried out on 36 guinea pigs using the SuperSonic Imagine Aixplorer scanner, and a 1 to 6 MH convex SC6-1 transducer. An ultrasound guided Try-Cut liver core needle biopsy was carried out in all the studied animals and the collected samples were examined to exclude pathological lesions. The mean liver tissue stiffness ranged from 0.89 to 5.40 kPa. We found that shear wave elastography is an easy, non-invasive technique that can be used to assess the stiffness of liver tissue. The obtained results can be used in future studies to assess the types and changes of liver tissue in the course of various types of liver disease.

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