Distribution Characteristics and Species Diversity of Bacteria in Hepatocellular Carcinoma Tissues

2022 ◽  
Vol 12 (1) ◽  
pp. 97-104
Author(s):  
Yeqing Fang ◽  
Weili Zhang
Keyword(s):  
Hepatocellular Carcinoma ◽  
Species Diversity ◽  
Liver Tissue ◽  
Bacterial Species ◽  
Normal Liver ◽  
Normal Group ◽  
Normal Liver Tissue ◽  
Control Group ◽  
Sequencing Data ◽  
Liver Tissues

This study was to explore the differences in the distribution and species diversity of bacteria between hepatocellular carcinoma (HCC) tissues and normal liver tissues. 28 HCC patients treated with surgery were selected as the research objects (HCC group), and 19 healthy volunteers with normal physical examinations were included in the control group (Normal group). The tumor specimens were obtained by intraoperative and biopsy puncture, and a 16S ribosomal ribonucleic acid (rRNA) library was constructed. Based on the sequencing data obtained by the IlluminaHi Seq sequencing platform, the differences of bacteria in the liver tissues of the HCC group and the Normal group were analyzed at the level of phyla, family, and genus. The Ace, Chao1, and Shannon of the two groups were compared. The results showed that IlluminaHi Seq sequencing obtained a total of 11,714,659 valid sequences, with an average of 131,625 sequences per sample. The proportions of Bacteroidetes, Firmicutes, and Proteobacteria in HCC group and Normal group were 48.75% versus 34.16%, 37.44% versus 18.02%, and 10.85% versus 39.26%, respectively. The Bacteroidaceae, Prevotellaceae, Lachnospiraceae, and Ruminococcaceae accounted for 22.49%, 20.62%, 16.54%, and 19.93% in Normal group; while those in the HCC tissues accounted for 26.83%, 14.22%, 11.14%, and 13.18%, respectively. The dominant bacteria at the genus level in HCC group and Normal group were Bacteroides and Prevotella-9, with the proportions of 24.19% versus 26.04% and 14.19% versus 8.44%, respectively. The difference in operational taxonomic unit (OTU) numbers of HCC and Normal group were compared and analyzed, which were 1,266 and 1,082, respectively, and the number of common OTU in the two tissues was 875. The Ace in HCC tissue and normal liver tissue were 1063.8±66.79 and 1003.6±52.19, respectively. The Ace in HCC tissue was greater than that in normal liver tissue (P < 0.05). The Chao1 and Shannon in HCC tissue were 1022.9±67.74 and 5.4269±0.3608, respectively; while those in normal liver tissue were 1003.6±66.79 and 5.2842±0.9714, respectively. The Chao1 and Shannon in HCC tissues were much higher than those in Normal group (P < 0.05). It showed that there was no difference in the types of bacterial species in HCC tissues, but the proportions of their flora at the level of phyla, family, and genus changed greatly, which may be related to the occurrence of HCC. This study could provide a reference for the diagnosis and treatment of HCC.

Elasticity Characterization of Liver Cancers Using Shear Wave Ultrasound Elastography: Comparison Between Hepatocellular Carcinoma and Liver Metastasis

2017 ◽  
Vol 33 (6) ◽  
pp. 481-488 ◽  
Author(s):  
Kah Lai Choong ◽  
Yin How Wong ◽  
Chai Hong Yeong ◽  
Gnana Kumar Gnanasuntharam ◽  
Khean Lee Goh ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Liver Metastasis ◽  
Shear Wave ◽  
Liver Tissue ◽  
Normal Liver ◽  
Ultrasound Elastography ◽  
Normal Liver Tissue ◽  
Tissue Elasticity ◽  
Liver Cancers

Purpose: This was a feasibility study of shear wave ultrasound elastography for characterization of liver tumors and to compare the tissue elasticity values of hepatocellular carcinoma (HCC), liver metastases, and normal liver tissues. Methods: Forty-one patients and 30 healthy volunteers were recruited and categorized into HCC, liver metastasis, and control groups based on their computed tomography and sonographic examinations. Elasticity values of different groups were compared statistically. Results: Mean (standard deviation) elasticity values for HCC, liver metastasis, and normal liver tissue were 51.45 (14.96), 49.89 (13.82), and 6.63 (1.65) kilopascal, respectively. Statistically significant differences were found between the elasticity values of HCC and liver metastasis with normal liver tissue. Based on the receiver operating characteristics analysis, 18.25 kilopascal may differentiate the malignant focal liver lesions from the normal liver tissue with both sensitivity and specificity of 100%. Conclusion: Shear wave ultrasound elastography may be able to differentiate HCC and liver metastasis from normal liver tissue based on the tissue elasticity values.

scholarly journals High expression of Janus kinase 2 in background normal liver tissue of resected hepatocellular carcinoma is associated with worse prognosis

2014 ◽  
Vol 33 (2) ◽  
pp. 767-773 ◽  
Author(s):  
FUMINORI SONOHARA ◽  
SHUJI NOMOTO ◽  
YOSHIKUNI INOKAWA ◽  
MITSUHIRO HISHIDA ◽  
NAO TAKANO ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Liver Tissue ◽  
Normal Liver ◽  
Janus Kinase ◽  
High Expression ◽  
Normal Liver Tissue ◽  
Janus Kinase 2 ◽  
Worse Prognosis

scholarly journals Distinct hepatitis B virus integration patterns in hepatocellular carcinoma and adjacent normal liver tissue

2017 ◽  
Vol 140 (6) ◽  
pp. 1324-1330 ◽  
Author(s):  
Xiaobo Yang ◽  
Liangcai Wu ◽  
Jianzhen Lin ◽  
Anqiang Wang ◽  
Xueshuai Wan ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Liver Tissue ◽  
Normal Liver ◽  
Normal Liver Tissue ◽  
B Virus ◽  
Virus Integration

scholarly journals FXYD6 overexpression in HBV-related hepatocellular carcinoma with cirrhosis

2020 ◽  
Vol 15 (1) ◽  
pp. 259-266
Author(s):  
Xiongfei Chen ◽  
Lishuang Ding ◽  
Deshuai Kong ◽  
Xiulei Zhao ◽  
Lili Liao ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Normal Liver ◽  
Early Recurrence ◽  
Tumor Diameter ◽  
Tumor Number ◽  
B Virus ◽  
Expression Rate ◽  
Polymerase Chain ◽  
And Gender ◽  
Liver Tissues

AbstractObjectiveThe aim of this study was to investigate the expression of FXYD domain-containing ion transport regulator 6 (FXYD6) mRNA and protein in hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC) tissues with cirrhosis, the corresponding paracancerous tissues and the normal liver tissues, and to explore the clinical significance of FXYD6 expression in HBV-related HCC with cirrhosis.MethodsThe FXYD6 mRNA and protein were examined by semi-quantitative reverse transcription polymerase chain reaction and immunohistochemistry, respectively.ResultsThe FXYD6 mRNA in HBV-related HCC tissues was significantly higher than that in the cirrhosis tissues or that in the normal liver tissues. The positive expression rate of FXYD6 protein was statistically higher in HBV-related HCC tissues than that in HBV-related cirrhosis or that in normal liver tissues. There was no significant correlation between the expression of FXYD6 protein and gender, age, histological differentiation, tumor diameter, tumor number, integrity of tumor capsule or not and alpha fetoprotein (AFP) concentration in serum, but the protein expression was associated with microvascular invasion, pathological stage, and early recurrence after operation within 1 year.ConclusionFXYD6 might be involved in hepatocyte carcinogenesis and tumor progression in HBV-related HCC with cirrhosis and indicated a poor prognosis.

scholarly journals Effect of Intrahepatic Arterial Delivery of Sorafenib on Normal Liver Tissue of Rabbit: An Experimental Study

2020 ◽  
Vol 3 (1) ◽  
pp. 1
Author(s):  
Eerdunbagena Ning ◽  
Zhijun Wang
Keyword(s):  
Heart Rate ◽  
Rectal Temperature ◽  
Liver Tissue ◽  
High Performance ◽  
Arterial Embolization ◽  
Hepatic Arterial Infusion ◽  
Normal Liver ◽  
Normal Liver Tissue ◽  
Breathing Rate ◽  
Local Necrosis

Objective: To assess the safety, feasibility and eluting efficiency of intrahepatic arterial delivery of sorafenib on normal liver tissue of rabbit. Methods: 24 New Zealand rabbits were randomly divided into three groups: group Ⅰ (Lipiodol-sorafenid), group Ⅱ (Lipiodol) and group Ⅲ (Sorafenib). Group Ⅰ and Ⅱ were treated by transcatheter selective hepatic arterial embolization with emulsions of lipiodol and sorafenib or with only lipiodol, while group Ⅲ was given hepatic arterial infusion with sorafenib. Sorafenib concentration in plasma was determined by HPLC (high performance liquid chromatography) in 0 min, 20 min, 1h, 2h, 4h, 8h, 16h, 32h and 48h respectively. The breathing rate, heart rate, rectal temperature and body weight were measured, as well the blood routine test and the function of liver, kidney, and heart. Two animals of each group were respectively killed in the 3rd day, 1st, 3rd and 6th week after treatment. Histopathologic study was done to liver, heart, kinney, lung, brain, gall bladder and intestine. Result: ① The peak sorafenib concentration (Cmax)and AUC(Area under curve) in plasma in groupⅠwas 2.46±0.101μg/ml and 945.72 ± 52.3 μg/mL.min respectively, while in group Ⅲ which was 3.78±0.180 ug/ml and 546.98±21.1μg/mL.min. Compared with groupⅢ, the Cmax and AUC of groupⅠhad a significant statistics difference (p<0.05). ② The breathing rate, heart rate, rectal temperature and AST/ALT,WBC,NEU% of group Ⅰand groupⅢhas a significant statistics difference(p<0.05) in the 3rd day. ③CK ,CK-MB, DB, Cr，BUN,RBC,PLT in plasma did not change in all group.④Local necrosis was seen in group Ⅰand group Ⅱin the 3rd day and 1st week, but they did not seem to be different. Group Ⅲ showed no necrosis. Granulation tissue with bile duty, portal vein and microfossils hyperplasia were seen in local necrosis area in the 3rd week. No pathological changes were found in brain, heart, kidney, intestine and gallbladder. Conclusion: TAE with emulsions of lipiodol and sorafenib is feasible, safe and has some slow-release effect.

Normal liver tissue change after proton beam therapy

2018 ◽  
Vol 36 (9) ◽  
pp. 559-565 ◽  
Author(s):  
Nobuyoshi Fukumitsu ◽  
Shinsei Takahashi ◽  
Toshiyuki Okumura ◽  
Toshiki Ishida ◽  
Keiko Nemoto Murofushi ◽  
...  
Keyword(s):  
Proton Beam ◽  
Liver Tissue ◽  
Normal Liver ◽  
Proton Beam Therapy ◽  
Normal Liver Tissue ◽  
Tissue Change

Metagenomic profiling of gut microbiota in urban and rural rats: A comparative study

2021 ◽  
Author(s):  
Rafig GURBANOV ◽  
Uygar KABAOĞLU ◽  
Tuba YAĞCI
Keyword(s):  
Species Richness ◽  
Species Diversity ◽  
Gut Microbiota ◽  
Bacterial Species ◽  
Rattus Rattus ◽  
Food Sources ◽  
Control Group ◽  
Laboratory Rats ◽  
Food Variety ◽  
Wild Rats

Abstract Mammals have a symbiotic relationship with various microorganisms called microbiota throughout their lives. These microorganisms are known to affect the host's physiology, health, and even mental balance. In the harbor of the densest and most diverse microorganisms in mammals, the curved structure of the intestines and their rich nutrient content are effective. The development of the gut microbiota is regulated by a complex interaction between host and environmental factors, including diet and lifestyle. Herein, it is aimed to elucidate the changes in the gut microbiota of rats living in urban and rural habitats. All taxonomic changes in the gut microbiota of wild rats belonging to Rattus rattus species caught from urban and rural areas of Western Anatolian (Bilecik province) were examined comparatively by 16S rRNA next-generation sequencing technique. Laboratory rats were used as a control group for comparison. Thus, 2000 different bacterial species were identified in gut microbiota. According to the Shannon and Simpsons values ​​calculated, laboratory rats showed the highest species diversity. When the similarities of microbiota profiles were compared with the principal coordinate analysis (PcoA), bacterial populations showed variability among different habitats. The comparison of species richness between the groups with the species rarefaction technique revealed higher species richness in all wild rats, especially in the rural habitat, compared to laboratory rats. Food sources were determined as the most important factor contributing to species richness and diversity. While the increased food variety boosted species richness, species diversity was increased due to the diminished food variety.

Study of the antineoplastic effect of modulating apoptosis-related noncoding RNA in human hepatocellular carcinoma cell line (HepG2)

QJM ◽  
2021 ◽  
Vol 114 (Supplement_1) ◽  
Author(s):  
Mai Abdel Azeem Sherif ◽  
Emtiaz Abd-elkawy Ismail ◽  
Samar Kamal Kassim ◽  
Hanan Hussein Shehata ◽  
Marwa Ali Abdel Khalek ◽  
...  
Keyword(s):  
Gene Expression ◽  
Hepatocellular Carcinoma ◽  
Hepg2 Cells ◽  
Noncoding Rna ◽  
Caspase 3 ◽  
Control Group ◽  
Antineoplastic Effect ◽  
And Control ◽  
Hcc Cells ◽  
Liver Tissues

Abstract MiR-421 is considered an important molecule that can prevent tumor growth. Bioinformatics analysis indicated that mRNA caspase-3 gene is a target gene of miR-421. The current study aimed to explore the functional role of miR-421 in hepatocellular carcinoma (HCC) and explore the interaction between miR-421 and caspase-3. To validate bioinformatics data, RT-qPCR was used to detect the expression of miR-421 and caspase-3 in 10 HCC tissues. The results showed miR-421 expression was significantly higher in HCC than non HCC liver tissues (P&lt;0.01), nevertheless caspase-3 gene expression was markedly lower in HCC than non HCC liver tissues (P&lt;0.01). Besides, miR-421 expression was negatively associated with caspase-3 expression. MiR-421 mimic and inhibitor was transfected into HCC cell lines (HepG2). Proliferation assay, showed that low-expression of miR-421 inhibited the proliferation of HCC cells. RT-qPCR was worked for detection the expression levels of miR-421 and caspase-3 in HepG2 cells before and after transfection. The results showed that miR-421 expression in HepG2 cells was significantly lower in miR-421 inhibitor transfected group than in mimic- transfected and control groups (Mock) (P≤ 0.05), and caspase-3 gene expression in HCC tissues was markedly higher in inhibitor transfected group than those transfected by mimic and control group (Mock) (P≤0.05). Thus, miR-421 inhibitor may inhibit the proliferation of HCC cells via over- expression of caspase-3.

Sign in / Sign up

Export Citation Format

Share Document