Two new lecanoroid lichen species from the forested wetlands of South Korea, with a key for Korean Protoparmeliopsis species

Lecanora parasymmicta Lee & Hur and Protoparmeliopsis crystalliniformis Lee & Hur are described as new lichen species to science from the forested wetlands in southern South Korea. Molecular analyses employing internal transcribed spacer (ITS) and mitochondrial small subunit (mtSSU) sequences strongly support the two lecanoroid species to be distinct in their genera. Lecanora parasymmicta is included in the Lecanora symmicta group. It is morphologically distinguished from Lecanora symmicta (Ach.) Ach., its most similar species, by areolate-rimose thallus, blackish hypothallus, larger apothecia, absence of thalline excipulum from the beginning, narrower paraphyses, larger ascospores, smaller pycnoconidia, and the presence of placodiolic acid. The second new species Protoparmeliopsis crystalliniformis is included in a clade with Protoparmeliopsis bipruinosa (Fink) S.Y. Kondr. and P. nashii (B.D. Ryan) S.Y. Kondr., differs from Protoparmeliopsis ertzii Bungartz & Elix, its most morphologically similar species, by whitish thallus, flat to concave and paler disc, longer ascospores, thallus K+ yellow reaction, presence of atranorin and rhizocarpic acid, and the substrate preference to sandstone or basalt. A key is provided to assist in the identification of Protoparmeliopsis species in Korea.

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Two new calcicolous caloplacoid lichens from South Korea, with a taxonomic key to the species of Huriella and Squamulea

MycoKeys ◽

10.3897/mycokeys.84.71227 ◽

2021 ◽

Vol 84 ◽

pp. 35-55

Author(s):

Beeyoung Gun Lee ◽

Jae-Seoun Hur

Keyword(s):

New Species ◽

South Korea ◽

Ribosomal Rna ◽

Internal Transcribed Spacer ◽

Large Subunit ◽

Small Subunit ◽

Similar Species ◽

Molecular Analyses ◽

Tip Cells ◽

Large Subunit Ribosomal Rna

Pyrenodesmia rugosa Lee & Hur and Huriella aeruginosa Lee & Hur are described as new lichen-forming fungi from a calcareous mountain of South Korea. Pyrenodesmia rugosa is distinguishable from Pyrenodesmia micromontana (Frolov, Wilk & Vondrák) Hafellner & Türk, the most similar species, by thicker thallus, rugose areoles, larger apothecia, shorter hymenium, shorter hypothecium and narrower tip cells of paraphyses. Huriella aeruginosa, the second new species, differs from ‘Squamulea’ chelonia Bungartz & Søchting by dark greenish-grey to grey thallus without pruina, gold to yellow-brown epihymenium, larger ascospores and thallus K– and KC– reaction. Molecular analyses employing internal transcribed spacer (ITS), mitochondrial small subunit (mtSSU) and nuclear large subunit ribosomal RNA (LSU) sequences strongly support the two caloplacoid species to be distinct in their genera. A surrogate key is provided to assist in the identification of all 20 taxa in Huriella and Squamulea.

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Arthonia ulleungdoensis, a New Lichenized Fungus from Ulleung Island, South Korea

Microorganisms ◽

10.3390/microorganisms7070205 ◽

2019 ◽

Vol 7 (7) ◽

pp. 205

Author(s):

Beeyoung Gun Lee ◽

Jae-Seoun Hur

Keyword(s):

New Species ◽

South Korea ◽

Rna Polymerase ◽

Northeast Asia ◽

Distinct Species ◽

Small Subunit ◽

Lichen Species ◽

Molecular Analyses ◽

Ulleung Island ◽

Lichenized Fungus

Arthonia ulleungdoensis Lee & Hur is described as a new lichen species from South Korea. The new species is distinguishable from Arthonia ruana A. Massal. by its large, rounded and non-punctiform apothecia, taller apothecial section, asci with fewer spores, and larger and permanently colorless spores. Molecular analyses employing mitochondrial small subunit (mtSSU) and RNA polymerase subunit II (RPB2) sequences strongly support Arthonia ulleungdoensis as a distinct species in the genus Arthonia. Overall, 22 Arthonia species are currently recorded in South Korea. A surrogate key is provided to assist in the identification of all 10 taxa of Arthonia/Arthothelium with muriform spores in Northeast Asia.

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A new lichenized fungus, Lecanora baekdudaeganensis, from South Korea, with a taxonomic key for Korean Lecanora species

MycoKeys ◽

10.3897/mycokeys.70.51569 ◽

2020 ◽

Vol 70 ◽

pp. 39-58

Author(s):

Beeyoung Gun Lee ◽

Jae-Seoun Hur

Keyword(s):

New Species ◽

South Korea ◽

Internal Transcribed Spacer ◽

Distinct Species ◽

Small Subunit ◽

Oil Droplets ◽

Molecular Analyses ◽

Taxonomic Key ◽

Lichenized Fungus

Lecanora baekdudaeganensis Lee & Hur is described as a new lichenized fungus from Baekdudaegan Mountains, South Korea. The new species is classified into the Lecanora subfusca group – allophana type and distinguishable from Lecanora imshaugii Brodo by a darker thallus, brownish disc, K–insoluble granules on the surface of the epihymenium, shorter hypothecium, and the presence of oil droplets in the apothecial section. Molecular analyses employing internal transcribed spacer (ITS) and mitochondrial small subunit (mtSSU) sequences strongly support Lecanora baekdudaeganensis as a distinct species in the genus Lecanora. A surrogate key is provided to assist in the identification of all 52 taxa in the genus Lecanora of Korea.

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Taxonomic revision of Russula subsection Amoeninae from South Korea

MycoKeys ◽

10.3897/mycokeys.75.53673 ◽

2020 ◽

Vol 75 ◽

pp. 1-29

Author(s):

Komsit Wisitrassameewong ◽

Myung Soo Park ◽

Hyun Lee ◽

Aniket Ghosh ◽

Kanad Das ◽

...

Keyword(s):

New Species ◽

South Korea ◽

Central America ◽

North American ◽

Internal Transcribed Spacer ◽

Phylogenetic Analyses ◽

Taxonomic Revision ◽

Molecular Phylogenetic ◽

Pileus Surface ◽

A New Species

Russula subsection Amoeninae is morphologically defined by a dry velvety pileus surface, a complete absence of cystidia with heteromorphous contents in all tissues, and spores without amyloid suprahilar spot. Thirty-four species within subsection Amoeninae have been published worldwide. Although most Russula species in South Korea have been assigned European or North American names, recent molecular studies have shown that Russula species from different continents are not conspecific. Therefore, the present study aims to: 1) define which species of Russula subsection Amoeninae occur on each continent using molecular phylogenetic analyses; 2) revise the taxonomy of Korean Amoeninae. The phylogenetic analyses using the internal transcribed spacer (ITS) and multilocus sequences showed that subsection Amoeninae is monophyletic within subgenus Heterophyllidiae section Heterophyllae. A total of 21 Russula subsection Amoeninae species were confirmed from Asia, Australia, Europe, North America, and Central America, and species from different continents formed separate clades. Three species were recognized from South Korea and were clearly separated from the European and North American species. These species are R. bella, also reported from Japan, a new species described herein, Russula orientipurpurea, and a new species undescribed due to insufficient material.

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Morphological and molecular characterisation of Xiphinema mazandaranense n. sp. (Dorylaimida: Longidoridae), a new member of the Xiphinema pyrenaicum species complex

Nematology ◽

10.1163/138855411x580768 ◽

2012 ◽

Vol 14 (1) ◽

pp. 109-119 ◽

Cited By ~ 5

Author(s):

Majid Pedram ◽

Ebrahim Pourjam ◽

Robert T. Robbins ◽

Weimin Ye ◽

Mohammad Reza Atighi ◽

...

Keyword(s):

New Species ◽

Species Complex ◽

Small Subunit ◽

Molecular Characterisation ◽

Molecular Analyses ◽

Small Subunit Rdna ◽

Group 6 ◽

Close Relationship ◽

Juvenile Stages ◽

Female Genital

Xiphinema mazandaranense n. sp. is described and illustrated. The new species belongs to morphospecies group 6, which is characterised by having two equally developed female genital branches that have spines in the uteri and a short, rounded tail. The new species is characterised by having spines in the tubular portion of the uterus, body 3.7-5.2 mm long, odontostyle 163-173 μm long, odontophore 96-100 μm long, body 62-80 μm diam., rounded tail, four juvenile stages, males rare with three ventromedian supplements next to the adcloacal pair and spicules 85 μm long. The polytomous identification codes of the new species are: A4-B3-C7b-D6-E456-F45-G4-H2-I23-J7b-K2-L1. The new species appears closely related to the members of X. pyrenaicum group which are characterised by a rounded tail with or without an inconspicuous projecting bulge and a uterus devoid of Z-differentiation but showing spiniform structures. The new species differs from members of the X. pyrenaicum group, which includes the recently described X. iranicum, by a more rounded tail without any projection. Beside morphological and morphometric data, molecular analyses of the near-full-length small subunit rDNA gene (SSU) placed the new species in close relationship with some species belonging to Xiphinema morphospecies group 6 and further separated this species from the X. pyrenaicum complex.

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Molecular and morphological characterisation of Aphelenchoides kheirii n. sp. (Nematoda: Aphelenchoididae) isolated from Pinus nigra in north-western Iran

Nematology ◽

10.1163/15685411-00003182 ◽

2018 ◽

Vol 20 (9) ◽

pp. 877-888

Author(s):

Behrouz Golhasan ◽

Ramin Heydari ◽

Mehrab Esmaeili ◽

Natsumi Kanzaki

Keyword(s):

New Species ◽

Large Subunit ◽

Pinus Nigra ◽

Small Subunit ◽

Similar Species ◽

Western Iran ◽

Lateral Lines ◽

Pine Trees ◽

Group 2 ◽

North Western

Summary Aphelenchoides kheirii n. sp. was isolated during a survey of nematodes associated with bark samples of pine trees (Pinus nigra) in West Azerbaijan province, north-western Iran. The new species has a body length of 500 (448-520) μm in the female and 442 (402-480) μm in the male. The cuticle is weakly annulated with four lateral lines and the lip region is offset. The stylet is 10.4 (10-11) μm long with small basal swellings and the excretory pore located immediately posterior to base of metacorpus; hemizonid invisible. The post-vulval uterine sac length 37.7 (28-45) μm. Spicules are relatively short (18-20 μm in dorsal limb) and the end of the dorsal limb clearly curved ventrally like a hook. The male tail has usual three pairs of caudal papillae (2 + 2 + 2) and a well-developed mucron. The female tail is conical, terminating in a complicated step-like projection, usually with many tiny nodular protuberances. The new species belongs to the Group 2 category of Aphelenchoides species sensu Shahina in which ten known species among Groups 2 and 4 sensu Shahina, namely: A. arcticus, A. blastophthorus, A. fuchsi, A. parasaprophilus, A. paraxui, A. xui, A. dactylocercus, A. gynotylurus, A. iranicus and A. saprophilus are the most similar species. Phylogenetic analysis based on small subunit (SSU) and partial large subunit (LSU) sequences of rRNA supported the morphological results.

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Morphometric and molecular analyses of Skrjabinolecithum pyriforme n. sp. (Digenea: Haploporidae) in mullet fish from the Primorsky Region, Russia

Journal of Helminthology ◽

10.1017/s0022149x16000626 ◽

2016 ◽

Vol 91 (5) ◽

pp. 625-632 ◽

Cited By ~ 7

Author(s):

V.V. Besprozvannykh ◽

D.M. Atopkin ◽

A.V. Ermolenko ◽

A.Yu. Beloded

Keyword(s):

New Species ◽

Oral Sucker ◽

Body Length ◽

Type Species ◽

Close Relationships ◽

The Body ◽

Similar Species ◽

Body Width ◽

Molecular Analyses ◽

A New Species

AbstractA new species of Skrjabinolecithum (Digenea: Waretrematinae), Skrjabinolecithum pyriforme n. sp., has been found in the intestines of Liza haematocheila and Mugil cephalus from the Primorsky Region, Russia. These worms differ from S. vitellosum and S. lobolecithum by the presence of two caeca, as do S. spasskii, S. indicum and S. bengalensis. These species differ morphologically from S. pyriforme n. sp. by a number of features, including body width, oral sucker, pharynx, eggs and ratio of length and width of the body. The most similar species to S. pyriforme n. sp. is Platydidymus flecterotestis (Zhukov, 1971) with some differences in maximal body length, testis and egg sizes. The results of molecular analysis confirmed that this new species belongs to Skrjabinolecithum on the basis of close relationships with S. spasskii-type species.

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Inocybe griseorubida, a new species of Pseudosperma clade from tropical India

Phytotaxa ◽

10.11646/phytotaxa.221.2.6 ◽

2015 ◽

Vol 221 (2) ◽

pp. 166 ◽

Cited By ~ 5

Author(s):

K. P. Deepna Latha ◽

Patinjareveettil Manimohan

Keyword(s):

Phylogenetic Analysis ◽

New Species ◽

Rna Polymerase Ii ◽

Internal Transcribed Spacer ◽

Large Subunit ◽

Similar Species ◽

Internal Transcribed Spacer Region ◽

Comprehensive Description ◽

Kerala State ◽

A New Species

Inocybe griseorubida sp. nov. is described from Kerala State, India. A comprehensive description, photographs, and comparisons with phenetically similar species are provided. The nuclear ribosomal internal transcribed spacer region (ITS), a portion of the nuclear ribosomal large subunit (nLSU) and a portion of the nuclear second-largest subunit of RNA polymerase II (rpb2) gene of this species were sequenced and analyzed. Phylogenetic analysis of rpb2 sequences confirmed both the novelty of the species and its placement within the Pseudosperma clade.

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Characterization of a Novel Hypotrich Ciliate From Heavy Metal-Contaminated Industrial Outlet in Onsan, Ulsan, South Korea

Frontiers in Microbiology ◽

10.3389/fmicb.2021.761961 ◽

2021 ◽

Vol 12 ◽

Author(s):

Santosh Kumar ◽

Daizy Bharti ◽

Shahed Uddin Ahmed Shazib ◽

Mann Kyoon Shin

Keyword(s):

New Species ◽

South Korea ◽

Phylogenetic Analyses ◽

Small Subunit ◽

Rrna Gene ◽

Small Subunit Rrna ◽

Gene Sequences ◽

A Cell ◽

The Right

Very few studies exist on the description of protozoan ciliates from industrially contaminated sites. In this study, we report a description of a novel hypotrich ciliate isolated from water samples collected from an industrially contaminated outlet in Onsan, Ulsan, South Korea. The oxytrichid ciliate, Histriculus tolerans n. sp., was investigated using live observation and protargol impregnation. The morphology, morphogenesis, and molecular phylogeny inferred from small-subunit (SSU) rRNA gene sequences were studied. The new species is mainly characterized by a cell size of about 70 × 40 μm in vivo, two elongate ellipsoidal macronuclear nodules and one or two micronuclei, adoral zone of about 51% of body length with 32 membranelles on average, about 34 cirri in the right and 24 cirri in the left marginal row, 18 frontoventral transverse cirri, six dorsal kineties including two dorsomarginal rows, and dorsal kinety 1 with 26 bristles. Morphogenesis is similar to that of the type species, i.e., Histriculus histrio, except that oral primordium does not contribute to anlage II of the proter. Phylogenetic analyses, based on small-subunit rRNA gene sequences, consistently place the new species within the family Oxytrichidae, clustering with H. histrio.

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The phylogeny of Ryocalanoidea (Copepoda, Calanoida) based on morphology and a multi-gene analysis with a description of new ryocalanoidean species

Zoological Journal of the Linnean Society ◽

10.1093/zoolinnean/zly069 ◽

2018 ◽

Vol 185 (4) ◽

pp. 925-957 ◽

Cited By ~ 2

Author(s):

Jasmin Renz ◽

Elena L Markhaseva ◽

Silke Laakmann

Keyword(s):

New Species ◽

Internal Transcribed Spacer ◽

Type Species ◽

Current System ◽

Gene Analysis ◽

Cytochrome Oxidase Subunit ◽

Molecular Analyses ◽

The Status ◽

New Interpretation ◽

Close Interrelationship

Abstract Two new species of ryocalanoid copepods (Crustacea: Calanoida), Ryocalanus squamatus sp. nov. and Yrocalanus kurilensis sp. nov. are described together with a female of Ryocalanus infelix Tanaka, 1956, type species for the genus Ryocalanus Tanaka, 1956, from abyssal depths in the Kurile-Kamchatka trench. The new species can be assigned to the superfamily Ryocalanoidea based on the segmentation and armature of the swimming legs and the modification of the male right antennule. A new interpretation of the fusions of segments in the male right antennule of Ryocalanus shows the marked differences between the ryocalanoidean genera. The status of Ryocalanoidea within the Calanoida is discussed based on morphology and a first molecular multi-gene analysis with cytochrome oxidase subunit I, cytochrome b, nuclear ribosomal 18S and 28S rDNA and internal transcribed spacer 2. This analysis supports the close interrelationship between Ryocalanoidea and Spinocalanoidea. The monophyletic status of Ryocalanoidea could not be retrieved in the phylogenetic analysis, as specimens of Yrocalanus formed a clade within Spinocalanoidea. The inconclusive results between morphological and molecular analyses are discussed with a proposition to keep the current system until more males of taxa belonging to the Spinocalanoidea are discovered, as the male antennule plays a crucial role in the interpretation of relationships between Ryocalanoidea and Spinocalanoidea.

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