Noninvasive Measurement of Skin Autofluorescence Is Increased in Patients with Systemic Sclerosis: An Indicator of Increased Advanced Glycation Endproducts?

Objective.Skin autofluorescence noninvasively assesses expression of advanced glycation endproducts and therefore potentially the presence of oxidative stress that is implicated in the pathogenesis of systemic sclerosis (SSc). We investigated whether autofluorescence was increased in patients with SSc, primary Raynaud’s phenomenon (RP), and morphea as compared to healthy controls.Methods.Measurements of autofluorescence were made at 5 upper limb sites in 16 healthy controls, 16 patients with diffuse cutaneous SSc (dcSSc), 15 with limited cutaneous SSc (lcSSc), 15 with primary RP, and 13 with morphea. For patients with morphea, additional measurements were made at the affected and an adjacent unaffected site.Results.Autofluorescence was significantly increased in patients with dcSSc but not lcSSc as compared to controls at the proximal phalanx [dcSSc median 0.15, interquartile range (IQR) 0.10–0.24, vs control 0.10, IQR 0.07–0.13; p = 0.014], dorsum of the hand (dcSSc 0.17, IQR 0.11–0.36, vs control 0.12, IQR 0.09–0.17; p = 0.031), the wrist (dcSSc 0.22, IQR 0.13–0.33, vs control 0.13, IQR 0.09–0.18; p = 0.005), and forearm (dcSSc 0.19, IQR 0.12–0.47, vs control 0.14, IQR 0.10–0.16; p = 0.022). There was a trend for autofluorescence to be increased in patients with lcSSc and at morphea sites, compared to noninvolved skin.Conclusion.Autofluorescence is increased in patients with dcSSc compared to primary RP and to healthy controls. This suggests increased oxidative stress and the potential for autofluorescence as a biomarker.

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Skin Autofluorescence, a Novel Marker for Glycemic and Oxidative Stress-Derived Advanced Glycation Endproducts: An Overview of Current Clinical Studies, Evidence, and Limitations

Diabetes Technology & Therapeutics ◽

10.1089/dia.2006.8.523 ◽

2006 ◽

Vol 8 (5) ◽

pp. 523-535 ◽

Cited By ~ 117

Author(s):

Douwe J. Mulder ◽

Tara Van De Water ◽

Helen L. Lutgers ◽

Reindert Graaff ◽

Rijk O. Gans ◽

...

Keyword(s):

Oxidative Stress ◽

Clinical Studies ◽

Advanced Glycation Endproducts ◽

Skin Autofluorescence ◽

Advanced Glycation ◽

Glycation Endproducts ◽

And Oxidative Stress

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Skin Autofluorescence, as Marker of Accumulation of Advanced Glycation Endproducts and of Cumulative Metabolic Stress, Is Not Increased in Patients with Systemic Sclerosis

International Journal of Rheumatology ◽

10.1155/2011/417813 ◽

2011 ◽

Vol 2011 ◽

pp. 1-6 ◽

Cited By ~ 4

Author(s):

M. E. Hettema ◽

H. Bootsma ◽

R. Graaff ◽

R. de Vries ◽

C. G. M. Kallenberg ◽

...

Keyword(s):

Risk Factors ◽

Cardiovascular Risk ◽

Systemic Sclerosis ◽

Cardiovascular Risk Factors ◽

Advanced Glycation Endproducts ◽

Renin Angiotensin System ◽

Skin Autofluorescence ◽

Advanced Glycation ◽

Angiotensin System ◽

Glycation Endproducts

Objective. To investigate whether advanced glycation endproducts (AGEs) in the skin are increased in patients with systemic sclerosis (SSc) and are related to the presence of disease-related and traditional cardiovascular risk factors.Methods. Skin autofluorescence, as a measure for the accumulation of AGEs, was assessed by measuring UV-A light excitation-emission matrices (AF-EEMS) in 41 SSc patients and 41 age- and sex-matched controls. Traditional cardiovascular risk factors and disease-related risk factors were recorded.Results. Skin AF-EEMS did not differ between SSc patients and controls (1.68±0.58 a.u. versus1.63±0.41 a.u.,P=0.684). Skin AF-EEMS in SSc patients was associated with levels of CRP (r=0.44,P=0.004), Medsger's severity scale (r=0.45,P=0.006), and use of agents intervening in the renin-angiotensin system (r=0.33,P=0.027). When analysing SSc patients and controls together, in multivariate analysis, only age and use of agents intervening in the renin-angiotensin system were independently associated with AF-EEMS.Conclusion. These data demonstrate that skin AGEs are not increased in SSc patients.

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Is skin autofluorescence (SAF) representative of dermal advanced glycation endproducts (AGEs) in dark skin? A pilot study

Heliyon ◽

10.1016/j.heliyon.2020.e05364 ◽

2020 ◽

Vol 6 (11) ◽

pp. e05364

Author(s):

Isabella M. Atzeni ◽

Jeltje Boersema ◽

Hendri H. Pas ◽

Gilles F.H. Diercks ◽

Jean L.J.M. Scheijen ◽

...

Keyword(s):

Pilot Study ◽

Advanced Glycation Endproducts ◽

Skin Autofluorescence ◽

Advanced Glycation ◽

Dark Skin ◽

Glycation Endproducts

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Beneficial effects of Chinese prescription Kangen-karyu on diabetes associated with hyperlipidemia, advanced glycation endproducts, and oxidative stress in streptozotocin-induced diabetic rats

Journal of Ethnopharmacology ◽

10.1016/j.jep.2009.04.032 ◽

2009 ◽

Vol 124 (2) ◽

pp. 263-269 ◽

Cited By ~ 15

Author(s):

Hyun Young Kim ◽

Takuya Okamoto ◽

Takako Yokozawa

Keyword(s):

Oxidative Stress ◽

Advanced Glycation Endproducts ◽

Diabetic Rats ◽

Advanced Glycation ◽

Glycation Endproducts ◽

Beneficial Effects ◽

And Oxidative Stress

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Metaflammation: Tissue-Specific Alterations of the NLRP3 Inflammasome Platform in Metabolic Syndrome

Current Medicinal Chemistry ◽

10.2174/0929867324666170407123522 ◽

2018 ◽

Vol 25 (11) ◽

pp. 1294-1310 ◽

Cited By ~ 23

Author(s):

Raffaella Mastrocola ◽

Manuela Aragno ◽

Giuseppe Alloatti ◽

Massimo Collino ◽

Claudia Penna ◽

...

Keyword(s):

Oxidative Stress ◽

Metabolic Syndrome ◽

Advanced Glycation Endproducts ◽

Low Grade ◽

Advanced Glycation ◽

Added Sugars ◽

Physiological Ageing ◽

Glycation Endproducts ◽

Inflammatory Status ◽

Age Related

In the last decades, the extension of life expectancy and the increased consumption of foods rich in saturated fats and added sugars have exposed the general population to emerging health problems. The prevalence of metabolic syndrome (MS), composed of a cluster of factors as obesity, dyslipidemia, hyperglycemia, and hypertension, is rapidly increasing in industrialized and developing countries leading to precocious onset of age-related diseases. Indeed, oxidative stress, accumulation of advanced glycation endproducts, and a chronic low-grade inflammation are common features of MS and physiological ageing. In particular, the entire set of MS factors contributes to the development of an inflammatory status named metaflammation, which has been associated with activation of early innate immune response through the assembling of the multiprotein complex inflammasome. The most investigated family of inflammasome platforms is the NOD-like receptor pyridine containing (NLRP) 3, which is activated by several exogenous and endogenous stimuli, leading to the sequential cleavage of caspase-1 and IL-1β, followed by secretion of active IL-1β. We here collect the most recent findings on NLRP3 activation in MS providing evidence of its central role in disease progression and organ dysfunction in target tissues of metaflammation, in particular in cardiovascular, hepatic and renal complications, with a focus on oxidative stress and advanced glycation endproducts. A wide overview of the most promising strategies for the modulation of NLRP3 activation and related metabolic repercussions is also provided, since the finding of specific pharmacological tools is an urgent requirement to reduce the social and economic burden of MS- and elderly-associated diseases.

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Banked RBCs Increase Endothelial Cell Oxidative Stress through Advanced Glycation Endproducts.

10.1164/ajrccm-conference.2009.179.1_meetingabstracts.a4006 ◽

2009 ◽

Author(s):

NS Mangalmurti ◽

SM Albelda ◽

AM Choi ◽

JS Lee

Keyword(s):

Oxidative Stress ◽

Endothelial Cell ◽

Advanced Glycation Endproducts ◽

Advanced Glycation ◽

Glycation Endproducts

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Advanced glycation endproducts mediate pro-inflammatory actions in human gestational tissues via nuclear factor-κB and extracellular signal-regulated kinase 1/2

Journal of Endocrinology ◽

10.1677/joe-06-0081 ◽

2007 ◽

Vol 193 (2) ◽

pp. 269-277 ◽

Cited By ~ 41

Author(s):

Martha Lappas ◽

Michael Permezel ◽

Gregory E Rice

Keyword(s):

Oxidative Stress ◽

Human Placenta ◽

Advanced Glycation Endproducts ◽

Nuclear Factor Κb ◽

Mitogen Activated Protein Kinase ◽

Nuclear Factor ◽

Advanced Glycation ◽

Glycation Endproducts ◽

Gestational Tissues

Processes of human labour include increased oxidative stress, formation of inflammatory mediators (e.g. cytokines) and uterotonic phospholipid metabolites (e.g. prostaglandins). In non-gestational tissues, advanced glycation endproducts (AGE) induce the expression of pro-inflammatory molecules through mitogen-activated protein kinase and nuclear factor κB (NF-κB)-dependent pathways. Thus, the aim of this study was to investigate the effects of AGE on 8-isoprostane (a marker of oxidative stress), pro-inflammatory cytokine and prostaglandin release in human gestational tissues, and to define the signalling pathways involved. Human placenta and gestational membranes (amnion and choriodecidua combined; n=5) were incubated in the absence or presence of AGE–BSA (0.25, 0.5, 1 and 2 mg/ml) for 18 h. AGE significantly increased in vitro release of tumour necrosis factor-α, interleukin (IL)-1β, IL-6, IL-8, prostaglandin (PG)E2, PGF2α and 8-isoprostane from human placenta and gestational membranes. This was associated with a concomitant increase in NF-κB p65 activation and ERK 1/2 phosphorylation. AGE-stimulated 8-isoprostane, cytokine and prostaglandin production was significantly suppressed by the ERK 1/2 inhibitor U0126 and the NF-κB inhibitor BAY 11-7082. In conclusion, AGE mediates inflammatory actions in human gestational tissues. Protein kinases and the NF-κB pathway play an essential role in AGE signalling in human gestational tissues.

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