scholarly journals Prevalence and antibiotic resistance of vancomycin-resistant enterococci in animal originated foods

2013 ◽  
Vol 37 ◽  
pp. 588-593 ◽  
Author(s):  
Figen ÇETİNKAYA ◽  
Tülay ELAL MUŞ ◽  
Gül Ece SOYUTEMİZ ◽  
Recep ÇIBIK
Keyword(s):  
Antibiotic Resistance ◽  
Vancomycin Resistant Enterococci ◽  
Vancomycin Resistant

scholarly journals High Frequency of Vancomycin-Resistant Enterococci in Sewage and Fecal Samples of Healthy Carriers

2019 ◽  
Vol 13 (1) ◽  
pp. 1-5 ◽  
Author(s):  
Fakhri Haghi ◽  
Neda Shirmohammadlou ◽  
Rabab Bagheri ◽  
Sama Jamali ◽  
Habib Zeighami
Keyword(s):  
Antibiotic Resistance ◽  
High Frequency ◽  
Municipal Sewage ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Fecal Samples ◽  
Vancomycin Resistant Enterococci ◽  
Agar Dilution ◽  
Vancomycin Resistant ◽  
Healthy Carriers

Objectives:Enterococci are part of the microbial flora of the gastrointestinal tract of animals and human and can be released into the environment through fecal materials. These microorganisms play an important role in the dissemination of antibiotic resistance genes. Vancomycin-Resistant Enterococci (VRE) have been obtained in municipal sewage, hospital and agricultural wastes and healthy carriers. The aim of this study was to investigate the frequency of VRE in sewage and fecal samples of healthy carriers.Methods:This study was performed on fecal specimens of 100 healthy carriers and 100 samples of sewage in Zanjan Province. Fecal and sewage samples were cultured on Trypticase Soy Agar and biochemical tests were performed for Enterococci identification. Antimicrobial susceptibility testing was performed as CLSI guidelines and vancomycin resistance was determined using the agar dilution method.Result:Of 200 cultured samples, 141 isolates of Enterococci were detected. 64 isolates were detected from fecal and 77 were isolated from the sewage samples. Antibiotic resistance profile of fecal isolates was as follows: tetracycline (57.8%), ciprofloxacin (54.7%), phosphomycin (54.7%), erythromycin (51.5%), chloramphenicol (12.5%), amoxicillin (21.8%) and gatifloxacin (23.5%). Also for the sewage samples, the most antibiotic resistance was detected against ciprofloxacin (76.6%) followed by tetracycline (74%), erythromycin (68.8%), phosphomycin (61%). According to Agar dilution method, among 141 isolates of Enterococci, 15 (10.6%) isolates were vancomycin resistant: 11 of sewage isolates (14.3%) and 4 of the carrier isolates (6.2%).Conclusion:Our study describes the high frequency of VRE in municipal sewage and healthy carriers. Regarding the importance of VRE strains in the clinical and environment, it seems necessary to follow up on the issue.

scholarly journals Survey of Antibiotic Resistance among Enterococci in North Rhine-Westphalia, Germany

1999 ◽  
Vol 37 (5) ◽  
pp. 1638-1641 ◽  
Author(s):  
R. R. Reinert ◽  
G. Conrads ◽  
J. J. Schlaeger ◽  
G. Werner ◽  
W. Witte ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Surveillance Study ◽  
Vancomycin Resistant Enterococci ◽  
Vancomycin Resistant ◽  
High Level ◽  
Resistance Rates

A surveillance study on antibiotic resistance of enterococcal isolates (n = 730) was carried out in North Rhine-Westphalia, Germany, in 1997. Resistance rates to ampicillin (7.4%), high-level gentamicin (15.0%), high-level streptomycin (27.9%), ciprofloxacin (37.9%), vancomycin (1.5%), and teicoplanin (1.5%) were determined. All vancomycin-resistant enterococci (VRE) carried the vanA gene. SmaI andApaI macrorestriction patterns indicated an intra- and interhospital spread of VRE.

scholarly journals In VivoandIn VitroEffects of a ClpP-Activating Antibiotic against Vancomycin-Resistant Enterococci

2018 ◽  
Vol 62 (8) ◽  
Author(s):  
Autumn Brown Gandt ◽  
Elizabeth C. Griffith ◽  
Ida M. Lister ◽  
Lisa L. Billings ◽  
Angel Han ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Surface Plasmon Resonance ◽  
Mechanisms Of Action ◽  
Surface Plasmon Resonance Analysis ◽  
Resistance Development ◽  
Content Type ◽  
Resonance Analysis ◽  
Vancomycin Resistant Enterococci ◽  
Activated State ◽  
Vancomycin Resistant

ABSTRACTAntibiotics with novel bactericidal mechanisms of action are urgently needed. The antibiotic acyldepsipeptide 4 (ADEP4) activates the ClpP protease and causes cells to self-digest. The effects of ADEP4 and ClpP activation have not been characterized sufficiently for the enterococci, which are important pathogens known for high levels of acquired and intrinsic antibiotic resistance. In the present study, ADEP4 was found to be potently active against bothEnterococcus faecalisandEnterococcus faecium, with MIC90s of 0.016 μg/ml and 0.031 μg/ml, respectively. ClpP purified fromE. faeciumwas found to bind ADEP4 in a surface plasmon resonance analysis, and ClpP activation by ADEP4 was demonstrated biochemically with a β-casein digestion assay. In addition,E. faeciumClpP was crystallized in the presence of ADEP4, revealing ADEP4 binding to ClpP in the activated state. These results confirm that the anti-enterococcal activity of ADEP4 occurs through ClpP activation. In killing curve assays, ADEP4 was found to be bactericidal against stationary-phase vancomycin-resistantE. faecalis(VRE) strain V583, and resistance development was prevented when ADEP4 was combined with multiple classes of approved antibiotics. ADEP4 in combination with partnering antibiotics also eradicated mature VRE biofilms within 72 h of treatment. Biofilm killing with ADEP4 antibiotic combinations was superior to that with the clinically used combinations ampicillin-gentamicin and ampicillin-daptomycin. In a murine peritoneal septicemia model, ADEP4 alone was as effective as ampicillin. ADEP4 coadministered with ampicillin was significantly more effective than either drug alone. These data suggest that ClpP-activating antibiotics may be useful for treating enterococcal infections.

scholarly journals Colonization of rectal mucosa by microbes with antibiotic resistance markers in children with hematological malignancies

2021 ◽  
Vol 4 (1) ◽  
pp. 90-97
Author(s):  
L.G. Boronina ◽  
◽  
E.V. Samatova ◽  
M.P. Kukushkina ◽  
S.A. Panova ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Hematological Malignancies ◽  
Rectal Mucosa ◽  
Modern Medicine ◽  
Antibiotic Resistant ◽  
Extended Spectrum Beta Lactamase ◽  
Carbapenem Resistant ◽  
Vancomycin Resistant Enterococci ◽  
Resistance Markers ◽  
Vancomycin Resistant

Background: the steadily increasing occurrence of the infections caused by antibiotic-resistant germs and the reduction in the efficacy of antimicrobials is one of the important issues of modern medicine. Aim: to study the colonization of rectal mucosa by extended-spectrum beta-lactamase (ESBL) — producing carbapenem-resistant Enterobacterales, carbapenem-resistant Pseudomonas aeruginosa, and vancomycin-resistant Enterococci using phenotypic methods and gene tests. Patients and Methods: this study was performed from December 10, 2019, to March 30, 2020. 150 samples (131 fecal specimens and 19 rectal swabs) collected from 66 patients who were admitted to the Hematological Center were examined. All samples were inoculated into selective chromogenic media. Results: 67 strains of Enterobacterales, 71 Enterococci, and 7 P. aeruginosa were isolated. Rectal colonization by ESBL-producing Enterobacterales was identified in 17 patients (25.8%). Е. coli (13 strains, 44,8%) and K. pneumoniae (10 strains, 34,5%) prevailed. In 5 children, two or more ESBL-producing strains were isolated, i.e., Е. coli plus K. pneumoniae (n=2), Е. coli plus E. cloacae (n=2), and Е. coli plus K. pneumoniae plus E. cloacae (n=1). The colonization by vancomycin-resistant Enterococci (predominantly E. faecium) was identified in 18 patients (27.3%). Vancomycin-resistant Enterococci plus ESBL-producing Enterobacterales were isolated in 9 patients (13.6%). Multidrugresistant strains were isolated among Е. coli (n=1), K. pneumoniae (n=3), and P. aeruginosa (n=1). Conclusions: our findings demonstrate that the colonization by one and two antibiotic-resistant microbe was seen in every forth patient (25.8– 27.3%) and every seventh patient (13.6%), respectively. Е. coli and K. pneumoniae prevailed among Enterobacterales and E. faecium prevailed among Enterococci. Chromogenic agars are designed to examine the specimens collected from patients. Antibiotic-resistant microbes may be identified 24 hours after receiving specimen at a laboratory when using these media. KEYWORDS: monitoring, colonization, resistance marker, microorganisms, children, hematological malignancies. FOR CITATION: Boronina L.G., Samatova E.V., Kukushkina M.P. et al. Colonization of rectal mucosa by microbes with antibiotic resistance markers in children with hematological malignancies. Russian Journal of Woman and Child Health. 2021;4(1):90–97. DOI: 10.32364/2618- 8430-2021-4-1-90-97.

Sign in / Sign up

Export Citation Format

Share Document