Characterisation of pH-shift-produced protein isolates from sago palm weevil (Rhynchophorus ferrugineus) larvae

2021 ◽  
pp. 1-12
Author(s):  
M. Chaijan ◽  
K. Chumthong ◽  
N. Kongchoosi ◽  
K. Chinarak ◽  
A. Panya ◽  
...  

Farm-raised sago palm weevil (Rhynchophorus ferrugineus) larvae (SPWL) can be used as a protein source for food sustainability. This study aimed to investigate the potential of pH-shift processing as a cold refinery approach to produce protein isolate from SPWL. Maximum solubility of SPWL protein was observed at pH 2.0 (acid-aided process) and pH 11.5 (alkaline-aided process). The zeta-potential of the protein solution was close to zero with the lowest solubility at pH 4.5. So, the protein precipitation was performed at this pH. Although both acid and alkaline methods yielded roughly 66% protein, their nutritional and techno-functional features differed based on the pH-shifting process. The alkaline-produced protein isolate had higher essential amino acid (EAA) content and EAA index but it was darker in colour. The acid-produced protein isolate had larger levels of umami-taste-active and functional amino acids, as well as a higher emulsifying capability.

2015 ◽  
Vol 44 (10) ◽  
pp. 1371-1375 ◽  
Author(s):  
KAH WAI YONG ◽  
AISYAH ABU BAKAR ◽  
WAHIZATUL AFZAN AZMI

2020 ◽  
pp. 1-10
Author(s):  
M. Chaijan ◽  
W. Panpipat

This study aimed at characterising the techno-biofunctional aspect of seasoning powder made from sago palm weevil larvae (SP) in comparison with commercial products prepared from pork (CP) and chicken (CC). SP had a comparable moisture and water activity with CP and CC, following the Thai Community Product Standards. SP had higher protein, fat, carbohydrate, calcium, magnesium and potassium with lower ash and sodium (P<0.05). All samples had the same Fourier transform infrared spectra with different peak intensities. SP was darker (lower L*, higher a* and b*, and lower whiteness) than CP and CC. Different content and polarity of the intermediate (A285) and final (A420) products of the Maillard reaction was found. A420 of the aqueous extract was distinctly higher than the acetone extract in all samples, suggesting the predominance of water soluble brown pigments. The highest total phenolic content and DPPH• inhibition was found in SP (P<0.05). The bulk density of SP was lower than CP and CC, which consequently affected the wettability. SP needed more time to become wet (P<0.05). The soup made from SP had the highest initial turbidity (P<0.05). All sensory aspects of SP were similar to CP and CC. Thus, SP can be categorised as an alternative functional food ingredient.


2020 ◽  
Vol 31 (4) ◽  
pp. 190-192
Author(s):  
H.A. El-Shafie ◽  
M.E. Mohammed ◽  
A.A. Sallam

Date palm offshoots represent an important source of planting material in many date palm-growing countries around the world. Infestation by the red palm weevil ((RPW) Rhynchophorus ferrugineus), the longhorn beetle ((LHB) Jebusaea hammerschmidti) and the rhinoceros beetle ((OB) Oryctes spp.) hinders commercialization and movement of these offshoots. An effective quarantine protocol, with exposure period of 72 h at 25 °C using ECO2FUME (EF) with phosphine concentration of 1500 ppm has been developed for date palm offshoots against these coleopteran internal tissue borers.


Insects ◽  
2021 ◽  
Vol 12 (7) ◽  
pp. 594
Author(s):  
Qian-Xia Liu ◽  
Zhi-Ping Su ◽  
Hui-Hui Liu ◽  
Sheng-Ping Lu ◽  
Bing Ma ◽  
...  

Red Palm Weevil (RPW), Rhynchophorus ferrugineus Olivier, is a notorious pest, which infests palm trees and has caused great economic losses worldwide. At present, insecticide applications are still the main way to control this pest. However, pesticide resistance has been detected in the field populations of RPW. Thus, future management strategies based on the novel association biological control need be developed. Recent studies have shown that the intestinal tract of RPW is often colonized by multiple microbial species as mammals and model insects, and gut bacteria have been found to promote the growth, development and immune activity of RPW larvae by modulating nutrient metabolism. Furthermore, two peptidoglycan recognition proteins (PGRPs), PGRP-LB and PGRP-S1, can act as the negative regulators to modulate the intestinal immunity to maintain the homeostasis of gut bacteria in RPW larvae. Here, we summarized the current knowledge on the gut bacterial composition of RPW and their impact on the physiological traits of RPW larvae. In contrast with metazoans, it is much easier to make genetic engineered microbes to produce some active molecules against pests. From this perspective, because of the profound effects of gut bacteria on host phenotypes, it is promising to dissect the molecular mechanisms behind their effect on host physiology and facilitate the development of microbial resource-based management methods for pest control.


Author(s):  
N. Alshammari ◽  
Meshari Alazmi ◽  
Naimah A. Alanazi ◽  
Abdel Moneim E. Sulieman ◽  
Vajid N. Veettil ◽  
...  

AbstractSeveral studies have investigated palm trees’ microbiota infected with red palm weevil (RPW) (Rhynchophorus ferrugineus), the major pest of palm trees. This study compared the microbial communities of infected and uninfected palm trees in the Hail region, Northern Saudi Arabia, determined by high-throughput 16S rRNA gene sequencing by Illumina MiSeq. The results indicated that taxonomic diversity variation was higher for infected tree trunk than the healthy tree trunk. Soil samples from the vicinity of healthy and infected trees did not have a significant variation in bacterial diversity. Myxococcota, Acidobacteriota, and Firmicutes were the dominant phyla in RPW-infected tree trunk, and Pseudomonadaceae was the most prominent family. This study is the first report on the characterization of RPW-infected and healthy palm trees’ microbiome.


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