Associations between Fusarium species and mycotoxins in oats and spring wheat from farmers’ fields in Norway over a six-year period

2016 ◽  
Vol 9 (3) ◽  
pp. 365-378 ◽  
Author(s):  
I.S. Hofgaard ◽  
H.U. Aamot ◽  
T. Torp ◽  
M. Jestoi ◽  
V.M.T. Lattanzio ◽  
...  
Keyword(s):  
Spring Wheat ◽  
Fusarium Species ◽  
Fusarium Culmorum ◽  
Fusarium Avenaceum ◽  
Published Data ◽  
Liquid Chromatography Mass Spectrometry ◽  
Fusarium Mycotoxins ◽  
Fusarium Spp ◽  
Cereal Grain ◽  
Relative Prevalence

During the last ten years, Norwegian cereal grain industry has experienced large challenges due to Fusarium spp. and Fusarium mycotoxin contamination of small-grained cereals. To prevent severely contaminated grain lots from entering the grain supply chain, it is important to establish surveys for the most prevalent Fusarium spp. and mycotoxins. The objective of our study was to quantify and calculate the associations between Fusarium spp. and mycotoxins prevalent in oats and spring wheat. In a 6-year period from 2004-2009, 178 grain samples of spring wheat and 289 samples of oats were collected from farmers’ fields in South East Norway. The grains were analysed for 18 different Fusarium-mycotoxins by liquid chromatography – mass spectrometry. Generally, the median mycotoxin levels were higher than reported in Norwegian studies covering previous years. The DNA content of Fusarium graminearum, Fusarium culmorum, Fusarium langsethiae, Fusarium poae and Fusarium avenaceum were determined by quantitative PCR. We identified F. graminearum as the main deoxynivalenol (DON) producer in oats and spring wheat, and F. langsethiae as the main HT-2 and T-2-toxins producer in oats. No association was observed between quantity of F. graminearum DNA and quantity of F. langsethiae DNA nor for their respective mycotoxins, in oats. F. avenaceum was one of the most prevalent Fusarium species in both oats and spring wheat. The following ranking of Fusarium species was made based on the DNA concentrations of the Fusarium spp. analysed in this survey (from high to low): F. graminearum = F. langsethiae = F. avenaceum > F. poae > F. culmorum (oats); F. graminearum = F. avenaceum > F. culmorum > F. poae = F. langsethiae (spring wheat). Our results are in agreement with recently published data indicating a shift in the relative prevalence of Fusarium species towards more F. graminearum versus F. culmorum in Norwegian oats and spring wheat.

scholarly journals Fusarium and mycotoxin content of harvested grain was not related to tillage intensity in Norwegian spring wheat fields

2020 ◽  
pp. 1-14
Author(s):  
I.S. Hofgaard ◽  
H.U. Aamot ◽  
T. Seehusen ◽  
H. Riley ◽  
R. Dill-Macky ◽  
...  
Keyword(s):  
Spring Wheat ◽  
Crop Residues ◽  
Fusarium Species ◽  
Fungal Growth ◽  
Soil Surface ◽  
Inoculum Potential ◽  
Head Blight ◽  
Fusarium Spp ◽  
Nutrient Runoff ◽  
Cereal Grain

To mitigate the risk of erosion and nutrient runoff, reduced tillage has become more prevalent in Norway. Within within recent decades, there have been some years with relatively high occurrence of Fusarium head blight and mycotoxins in Norwegian cereal grain. This is thought to have been caused by an increased inoculum potential (IP) of Fusarium spp. due to larger amount of crop residues remaining on the soil surface, in combination with weather conditions promoting fungal growth and infection of cereal plants. The objective of this work was to elucidate the influence of different tillage practices on the IP of Fusarium spp. and the subsequent Fusarium-infection and mycotoxin contamination of spring wheat grain at harvest. Tillage trials were conducted at two locations in southeast Norway (Solør and Toten) over three years, 2010-2012. Residues of wheat from the previous year were collected in spring. Fusarium avenaceum and Fusarium graminearum were the most common Fusarium species recorded on wheat straw residues. IP was calculated as the percentage of the residues infested with Fusarium spp. multiplied by the proportion of the soil surface covered with residues. The IP of Fusarium spp. was lower in ploughed plots compared to those tilled with harrowing only. Ploughing in spring resulted in a similarly low IP as autumn ploughing. In contrast, harrowing in autumn generally reduced IP more than did spring harrowing. The mycotoxin levels in the harvested wheat were generally low, except for deoxynivalenol at high levels in Solør 2011. Despite a lower IP of ploughed versus harrowed plots, this was not reflected in the content of Fusarium and mycotoxins in harvested grain. The Fusarium species that dominated in the residues examined in this study were the same as those detected in the harvested grain, supporting the finding that residues are an important source of inoculum.

scholarly journals Effects of Antagonists on Mycotoxins of Seedborne Fusarium spp. in Sweet Corn

Toxins ◽  
2019 ◽  
Vol 11 (8) ◽  
pp. 438
Author(s):  
Mary E. Ridout ◽  
Bruce Godfrey ◽  
George Newcombe
Keyword(s):  
Sweet Corn ◽  
Fusarium Species ◽  
Fusarium Mycotoxins ◽  
Fusarium Spp ◽  
Corn Seed ◽  
Pichia Membranifaciens ◽  
Mature Seeds ◽  
F1 Cross

Fusarium species coexist as toxigenic, systemic pathogens in sweet corn seed production in southwestern Idaho, USA. We hypothesized that fungal antagonists of seedborne Fusarium would differentially alter production of Fusarium mycotoxins directly and/or systemically. We challenged the Fusarium complex by in vitro antagonism trials and in situ silk and seed inoculations with fungal antagonists. Fungal antagonists reduced growth and sporulation of Fusarium species in vitro from 40.5% to as much as 100%. Pichia membranifaciens and Penicillium griseolum reduced fumonisin production by F. verticillioides by 73% and 49%, respectively, while P. membranifaciens and a novel Penicillium sp. (WPT) reduced fumonisins by F. proliferatum 56% and 78%, respectively. In situ, pre-planting inoculation of seeds with Penicillium WPT systemically increased fumonisins in the resulting crop. Morchella snyderi applied to silks of an F1 cross systemically reduced deoxynivalenol by 47% in mature seeds of the F2. Antagonists failed to suppress Fusarium in mature kernels following silk inoculations, although the ratio of F. verticillioides to total Fusarium double with some inoculants. Fusarium mycotoxin concentrations in sweet corn seed change systemically, as well as locally, in response to the presence of fungal antagonists, although in Fusarium presence in situ was not changed.

STUDIES ON FOOT AND ROOT ROT OF WHEAT: IV. EFFECT OF CROP ROTATION AND CULTURAL PRACTICE ON THE RELATIVE PREVALENCE OF HELMINTHOSPORIUM SATIVUM AND FUSARIUM SPP. AS INDICATED BY ISOLATIONS FROM WHEAT PLANTS

1934 ◽  
Vol 10 (1) ◽  
pp. 115-124 ◽  
Author(s):  
W. C. Broadfoot
Keyword(s):  
Root Rot ◽  
Cultural Practices ◽  
Fusarium Culmorum ◽  
Cultural Practice ◽  
Root Tissue ◽  
Fusarium Spp ◽  
Crown Tissue ◽  
Helminthosporium Sativum ◽  
Relative Prevalence ◽  
Marked Tendency

The crown and root tissue from 43,305 of 47,360 plants examined in this investigation yielded Helminthosporium sativum, Fusarium culmorum and other Fusarium spp., either alone or in combination with these or other fungi and bacteria. It was the exception for any mature plant, the surface tissue of which was disinfected, to be free from fungi or bacteria. None of the various crop sequences or cultural practices used in this study appeared to significantly affect more than another the relative prevalence of either H. sativum or Fusarium spp., as indicated by isolations from the crown tissue of wheat. However, as there was a marked tendency at certain stations each year for H. sativum or Fusarium spp. to predominate, it was concluded that certain factors of the environment were more effective than the crop sequence in modifying the relative prevalence of the two fungi mentioned in the crown and root tissue of wheat plants.

Fungal Infection and Presence of Deoxynivalenol and Zearalenone in Cereals Grown in The Netherlands

1996 ◽  
Vol 59 (7) ◽  
pp. 772-777 ◽  
Author(s):  
MONIQUE DE NIJS ◽  
POP SOENTORO ◽  
ELLEN DELFGOU-VAN ASCH ◽  
HENRY KAMPHUIS ◽  
FRANK M. ROMBOUTS ◽  
...  
Keyword(s):  
The Netherlands ◽  
Fungal Infection ◽  
Fusarium Species ◽  
Fusarium Culmorum ◽  
Weather Conditions ◽  
Species Level ◽  
Fusarium Mycotoxins ◽  
Genus Fusarium ◽  
Fusarium Crookwellense ◽  
Cereal Samples

In 1991 and 1993 cereals were sampled during harvest in The Netherlands. The samples were tested for the presence of molds and the samples of 1993 were additionally tested for the mycotoxins deoxynivalenol and zearalenone. The molds were identified to genus level and those belonging to the genus Fusarium to species level. The total fungal infection of cereals in 1991 did not differ from 1993, with a median value of 5.0 log CFU g−1 in both years. The incidences of the genera Aspergillus, Penicillium, the group of Mucor and Rhizopus, Cladosporium, and Fusarium differed considerably between the two years, possibly caused by the different weather conditions. The numbers of samples infected with Fusarium were much higher in 1993 (83%) than in 1991 (34%). In 1991, no Fusarium was detected in samples from the southern part of The Netherlands, as opposed to 1993, when Fusarium was found in all regions sampled. The most dominant Fusarium species in 1991 were Fusarium culmorum and Fusarium avenaceum. In 1993, Fusarium poae, Fusarium culmorum, and Fusarium crookwellense dominated. All these Fusarium species are known mycotoxin producers. Three percent of the cereal samples of 1993 contained deoxynivalenol and 1% contained zearalenone in levels of over 500 μg kg−1 and 200 μg kg−1, respectively. This study has shown that the incidences of various fungal genera and Fusarium species in cereals in The Netherlands can vary from year to year. Considerable numbers of toxigenic Fusarium molds can occur and Fusarium mycotoxins may be present.

What about the 'other' Fusarium mycotoxins?

2009 ◽  
Vol 2 (2) ◽  
pp. 181-192 ◽  
Author(s):  
M. Jestoi ◽  
M. Kokkonen ◽  
S. Uhlig
Keyword(s):  
Current Knowledge ◽  
Fusarium Species ◽  
Animal Health ◽  
Natural Occurrence ◽  
Fusarium Mycotoxins ◽  
Fusarium Spp ◽  
Natural Toxins ◽  
Toxic Metabolites ◽  
Food And Feed ◽  
Very High

Most Fusarium species are capable of producing mycotoxins that may cause adverse effects on human or animal health. The most commonly studied Fusarium mycotoxins include trichothecenes, zearalenone and fumonisins. However, it seems that nearly all of the most prevalent Fusarium species infecting grains are also capable of producing other toxic metabolites. The existing studies, although exiguous, have clearly demonstrated that other toxic metabolites of Fusarium spp. are also present in our foods and feeds, occasionally at very high levels. It is apparent that since mycotoxins, including these 'other' metabolites, are natural toxins, they cannot be completely eliminated from food and feed chains. However, scientific studies are needed to determine their true significance. Thus, the mechanism and level of toxicity as well as presence and concentration levels will have to be fully clarified. In this paper, we briefly review the prevalence of the dominant Fusarium species contaminating maize and small-grain cereals worldwide, and the current knowledge on the biological activity as well as the natural occurrence of their selected less-known toxic metabolites. Additionally, the significance of these 'other' Fusarium mycotoxins is discussed.

Trichothecene and Moniliformin Production by Fusarium Species from Western Canadian Wheat†

2001 ◽  
Vol 64 (8) ◽  
pp. 1220-1225 ◽  
Author(s):  
D. ABRAMSON ◽  
R. M. CLEAR ◽  
D. GABA ◽  
D. M. SMITH ◽  
S. K. PATRICK ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Liquid Chromatography ◽  
Culture Medium ◽  
Culture Media ◽  
Fusarium Species ◽  
Fusarium Culmorum ◽  
Fusarium Avenaceum ◽  
Gas Chromatography Mass Spectrometry ◽  
Western Canada

Fusarium graminearum, Fusarium culmorum, and Fusarium avenaceum, isolated from Fusarium-damaged wheat harvested in western Canada, were cultured and evaluated for mycotoxin production. Extracts of the culture media were assayed for trichothecenes by gas chromatography-mass spectrometry and for moniliformin by liquid chromatography. Deoxynivalenol (DON) was found in 28 of 42 isolates of F. graminearum and 42 of 42 isolates of F. culmorum at levels ranging from 0.5 to 25.0 μg/g. 15-AcetylDON was found in 28 of 42 isolates of F. graminearum at levels ranging from 1.0 to 7.1 μg/g. 3-AcetylDON was found in 41 of 42 isolates of F. culmorum at levels ranging from 0.8 to 13.0 μg/g. Several other trichothecenes were assayed but not detected in the culture medium. Moniliformin was present in 40 of 42 isolates of F. avenaceum at levels ranging from 1.3 to 138.1 μg/g, but was not present in any of the isolates of F. graminearum or F. culmorum.

Fusarium species, zearalenone and deoxynivalenol content in preharvest scabby wheat heads from Poland

2012 ◽  
Vol 5 (2) ◽  
pp. 133-141 ◽  
Author(s):  
J. Chełkowski ◽  
K. Gromadzka ◽  
Ł. Stępień ◽  
L. Lenc ◽  
M. Kostecki ◽  
...  
Keyword(s):  
Fusarium Species ◽  
Fusarium Culmorum ◽  
Microdochium Nivale ◽  
Fusarium Avenaceum ◽  
Head Blight ◽  
Southern Poland ◽  
High Incidence ◽  
Identification Of Species ◽  
Species Specific ◽  
First Time

High incidence of Fusarium head blight occurred in Northern and Southern Poland in the 2009 season. Head samples from 106 wheat fields were collected before harvest from Northern, Central and Southern Poland in August 2009. Fusarium species were identified in 1,311 heads with visible scab symptoms and the collected material was subjected to mycotoxin analyses. Fusarium graminearum was identified as the most frequently occurring species on wheat, present in 48% of all samples examined. This species prevailed in Northern and Southern Poland, with the frequencies of 53% and 55%, respectively, and its frequency has increased over five-fold after two decades. In the central part of the country, Fusarium culmorum was the major pathogen of wheat, with a frequency of 43%, although in this region the incidence of infected heads in wheat fields was lower than 1%. Several other species, including Fusarium avenaceum, Fusarium cerealis and Microdochium nivale, occurred with lower frequencies. Microscopic identification of species was confirmed using species-specific markers in DNA extracted directly from sporodochia. For the first time, glucosylated deoxynivalenol was identified in Polish cereals, in amounts of 1.6 to 7.4 mg/kg. Deoxynivalenol (DON) content was estimated between 1.7 and 11.9 mg/kg for the healthy looking kernels (HLK) fraction, while the Fusarium-damaged kernels (FDK) were contaminated with high amounts of DON, from 57.3 to 312.3 mg/kg, and zearalenone, from 0.035 to 4.48 mg/kg. The HLK fractions contained about 20 times less DON and zearalenone (ZEA) than the FDK fractions. ZEA accumulated in both FDK kernels and chaff fractions at a similar level. DON was accumulated in the chaff fraction in much lower amounts than in the FDK fraction.

scholarly journals Survey of Fusarium Species Associated with Fusarium Head Blight of Spring Wheat (Triticum aestivum) in Southeastern Idaho

2018 ◽  
Vol 19 (2) ◽  
pp. 125-127 ◽  
Author(s):  
Kaitlyn M. Bissonnette ◽  
Philip Wharton ◽  
Jianli Chen ◽  
Juliet M. Marshall
Keyword(s):  
Triticum Aestivum ◽  
Fusarium Head Blight ◽  
Spring Wheat ◽  
Management Practices ◽  
Fusarium Species ◽  
Fusarium Culmorum ◽  
Head Blight ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Species Specific

In Idaho, losses due to Fusarium head blight (FHB) of spring wheat (Triticum aestivum) have been infrequent and have historically been dominated by Fusarium culmorum (Wm. G. Sm.) Sacc. However, the incidence of FHB and deoxynivalenol-contaminated grain has increased in spring wheat in southeastern Idaho since 2009, indicating that other species of Fusarium may be contributing to disease. In 2011 and 2012, 17 spring wheat fields were scouted and sampled for FHB in southern Idaho. Contaminated grains were cultured, and putative Fusarium isolates were identified using species-specific polymerase chain reaction. In 2011, 87% of all recovered isolates were identified as F. graminearum, whereas only 13% were identified as F. culmorum. Of the isolates collected in 2012, 51% were identified as F. graminearum and 49% as F. culmorum. In both years, more F. graminearum isolates were recovered as compared to a survey conducted in 1984. Implementation of effective disease management practices will be necessary to minimize the establishment and spread of F. graminearum–responsible FHB in southeastern Idaho.

scholarly journals FUNGI OCCURRED ON MARIGOLD (Tagetes L.) AND HARMFULNESS OF Fusarium SPECIES TO SELECTED CULTIVARS

2019 ◽  
Vol 18 (2) ◽  
Author(s):  
Elżbieta Mielniczuk ◽  
Małgorzata Cegiełko
Keyword(s):  
Rhizoctonia Solani ◽  
Fusarium Species ◽  
Fusarium Culmorum ◽  
Disease Index ◽  
Root Infection ◽  
Fusarium Spp ◽  
Flowering Stage ◽  
Disease Symptoms ◽  
Mean Values ◽  
The Mean

In Poland, cultivars belonging to three species of Tagetes are grown: T. erecta, T. patula, T. tenuifolia. Presented studies were conducted in 2015 in two localities of the Lublin region. Those studies included cultivars of Tagetes spp.: T. erecta (cv. ‘Alaska’, ‘Hawaii’, ‘Mann Im Mond’), T. patula (cv. ‘Bolero’ and ‘Carmen’) and T. tenuifolia (cv. ‘Lemon Gem’ and ‘Lulu’). Six weeks after the sowing and in flowering stage of analyzed marigold cultivars, the plants healthiness was assessed. The mean values of the disease index ranged from 16.25 to 29.0. The main cause of root infection at the seedling stage proved to be the species of Fusarium and Rhizoctonia solani. Proportion of plants with disease symptoms in flowering stage ranged from 1.25 to 5.5%. Regardless of the place of cultivation, species: A. alternata, F. culmorum and F. equiseti were the most often isolated from diseased plants in the flowering stage. Studies on the susceptibility of analyzed cultivars of marigold to infection by Fusarium culmorum, F. equiseti and F. sporotrichioides were conducted in a growth chamber. Significantly, the lowest value of the disease index of cultivar ‘Bolero’ (T. patula) allows to accept that cultivar as the least susceptible to infestation by Fusarium spp. under conditions of controlled temperature and humidity

The effect of hull removal and pearling on Fusarium species and trichothecenes in hulless barley

1997 ◽  
Vol 77 (1) ◽  
pp. 161-166 ◽  
Author(s):  
R. M. Clear ◽  
S. K. Patrick ◽  
T. Nowicki ◽  
D. Gaba ◽  
M. Edney ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Hordeum Vulgare ◽  
Fusarium Species ◽  
Fusarium Avenaceum ◽  
Head Blight ◽  
Fusarium Spp ◽  
Hordeum Vulgare L ◽  
Hulless Barley ◽  
Original Weight ◽  
Fusarium Poae

Fourteen samples consisting of three cultivars of hulless barley (Hordeum vulgare L.), grown commercially in southern Manitoba in 1994, were examined for levels of Fusarium species and seven fusarium trichothecene mycotoxins: deoxynivalenol (DON), diacetoxyscirpenol, 15-acetyldeoxyni-valenol (15ADON), fusarenone-X, HT-2 toxin, neosolaniol and T-2 toxin. Four fractions from each sample were analysed. These consisted of kernels with the hulls still attached, kernels whose hulls had been removed at harvest, kernels that had been dehulled in the laboratory using a deawner, and kernels that had been pearled in the laboratory to 60% of their original weight. A composite sample of the hulls, obtained from the laboratory dehulling, was also tested. Mycotoxins were determined by gas chromatog-raphy-mass spectrometry. DON and 15ADON were the only trichothecenes detected in this study. The greatest numbers of propagules of Fusarium spp. and the highest concentrations of DON and 15ADON were found in the hulls. The average concentrations of F. graminearum Schwabe and DON in barley were reduced: 90 and 49%, respectively, as a consequence of laboratory dehulling; 95 and 59%, respectively, as a result of dehulling during harvest; and 99 and 86%, respectively, as a result of pearling. Results suggest that growing hulless cultivars shows promise for management of mycotoxin problems associated with FHB. Key words: Fusarium graminearum, Fusarium poae, Fusarium avenaceum, deoxynivalenol, 15-acetyldeoxynivalenol, fusarium head blight

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