Prolactin Genotyping of Najdi Cattle Breed Using PCR-RFLP

Abstract Leptin, a biomolecule secreted by adipose tissue, enchances productivity in cattle, especially affecting milk traits. The aim of this study was to detect leptin gene polymorphism on exon 3 (A59V locus) and intron 2 (SAU3AI locus) in the endangered population of autochtonous Busha cattle and associations with milk traits. The study included 46 cows: 36 Busha and 10 half-bred. Milk analyses comprised determination of somatic cell counts, fat, protein, lactose, total solids and solids-not-fat (SNF) concentrations and freezing point depression (FPD). Polymorphisms were determined by PCR-RFLP technique. A single A59V genotype (CC) was affirmed, and two SAU3AI genotypes, AA and AB, with frequencies of 78.26% and 21.74%, respectively. Comparing the obtained results for chemical characteristics of milk between cows with AA and AB, no significant differences were found, except for SNF content and FPD values. Cows with AA genotype had significantly lower (p=0.021) average SNF content (8.74%) in milk compared to the average SNF content (9.28%) in those with genotype AB, while cows with genotype AA (−0.54°C) had significantly higher (p=0.004) average FPD values than those with AB genotype (−0.58°C). The absence of BB genotype and significant differences in the investigated functional traits between two SAU3AI genotypes and the absence of A59V polymorphism (presence of only CC genotype) show that the Busha cattle breed, although being an autochtonous low-producing native breed used for meat and milk production, harbours polymorphism on gentic markers characteristic of high production dairy cows.

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PSXII-34 The evaluation of the influence of FMO3 gene polymorphism on the milk productivity of Ayrshire breed cows

Journal of Animal Science ◽

10.1093/jas/skaa278.452 ◽

2020 ◽

Vol 98 (Supplement_4) ◽

pp. 249-250

Author(s):

Elena Gladyr` ◽

Elena Konovalova ◽

Nikolay Sivkin ◽

Olga V Kostyunina

Keyword(s):

Gene Polymorphism ◽

Milk Yield ◽

Milk Protein ◽

Cattle Breed ◽

Monogenic Disease ◽

World Population ◽

Milk Productivity ◽

Pcr Rflp ◽

Ayrshire Breed ◽

Significant Superiority

Abstract Russian Ayrshire population of cattle is the second largest in number the world population of animals of this breed. Trimethylaminuria (TMA) is the monogenic disease manifesting in the ‘‘fish off-flavor” of the milk from the sick cows and caused by R238X mutation in the flavin-containing monooxidase 3 gene (FMO3). The aim of our investigation was the analysis of the FMO3 gene polymorphism of Ayrshire cattle breed and its relationship with the milk productivity of the first lactation. The analysis of FMO3 gene polymorphism has been carried out on 352 animals of 5 herds, and associations with the milk productivity due 305 days have been evaluated on 20 healthy cows (RR) and 6 ones-carriers of the mutation (RX). DNA has been extracted by means of the kit “DNA-Extran” (“Syntol”, Russia). The identification of the FMO3 genotypes has been conducted by PCR-RFLP in according to the previously developed method with using TaqI restriction endonuclease. The frequency of animals-carriers of X allele associated with the defect of “fish off-flavor” of milk was in the range 10.21–12.82%. The frequencies of RR, RX and XX genotypes were 76.9, 20.5 and 2.6 %, respectively. The study of relationship between the genotypes on FMO3 gene and productivity traits has shown that the homozygous RR-individuals in comparing to RX-ones had significantly higher levels of the milk yield (+918.6 kg, P ≥ 0.05) and content of the milk protein (+28.8, P ≥ 0.05). Adaptive abilities of RR-cows of Russian selection allowed them to surpass animals of Finnish origin with a similar genotype in milk yield 5,655.6 ± 254.2 against 5,105.0 ± 238.6 kg. The investigations has shown the significant superiority the animals-non carriers of the “fish off-flavor” defect in the milk yield and content of the milk protein. This research was supported by the Ministry of science and higher education АААА-А18-118021590138-1.

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Growth Hormone Genotyping of Najdi Cattle Breed Using PCR-RFLP

Journal of Animal and Veterinary Advances ◽

10.3923/javaa.2010.1265.1266 ◽

2010 ◽

Vol 9 (8) ◽

pp. 1265-1266

Author(s):

A. Rastegari ◽

H. Roshanfekr ◽

M. Mamouie ◽

S.R. Khatami

Keyword(s):

Growth Hormone ◽

Cattle Breed ◽

Pcr Rflp

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GROWTH HORMONE GENE GENOTYPING BY Msp I RESTRICTION ENZYME AND PCR-RFLP METHODS IN ACEH CATTLE BREED AT INDRAPURI DISTRICT OF ACEH PROVINCE

Journal of the Indonesian Tropical Animal Agriculture ◽

10.14710/jitaa.38.4.207-211 ◽

2013 ◽

Vol 38 (4) ◽

Author(s):

W.P.B. Putra ◽

T. Hartatik ◽

S. Sumadi

Keyword(s):

Growth Hormone ◽

Restriction Enzyme ◽

Cattle Breed ◽

Growth Hormone Gene ◽

Pcr Rflp ◽

Aceh Province

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Distribution ofBoLA-DRB3Allelic Frequencies and Identification of Two New Alleles in Iranian Buffalo Breed

The Scientific World JOURNAL ◽

10.1100/2012/863024 ◽

2012 ◽

Vol 2012 ◽

pp. 1-6 ◽

Cited By ~ 1

Author(s):

J. Mosafer ◽

M. Heydarpour ◽

E. Manshad ◽

G. Russell ◽

G. E. Sulimova

Keyword(s):

Immune Response ◽

Major Histocompatibility Complex ◽

Disease Resistance ◽

Genetic Variability ◽

Cattle Breed ◽

Histocompatibility Complex ◽

Pcr Rflp ◽

Attractive Candidate ◽

New Alleles

The role of the major histocompatibility complex (MHC) in the immune response makes it an attractive candidate gene for associations with disease resistance and susceptibility. This study describes genetic variability in theBoLA-DRB3in Iranian buffaloes. Heminested PCR-RFLP method was used to identify the frequency ofBoLA-DRB3alleles. TheBoLA-DRB3locus is highly polymorphic in the study herd (12 alleles). Almost 63.50% of the alleles were accounted for by four alleles (BoLA-DRB3.2 *48,*20,*21, andobe) in Iranian buffalo. TheDRB3.2 *48allele frequency (24.20%) was higher than the others. The frequencies of theDRB3.2 *20andDRB3.2 *21are 14.52 and 14.00, respectively, andobeandgbbhave a new pattern. Significant distinctions have been found between Iranian buffalo and other cattle breed studied. In the Iranian buffaloes studied alleles associated with resistance to various diseases are found.

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Growth hormone genotyping by MspI restriction enzyme and PCR-RFLP method in Aceh cattle breed at Indrapuri District, Aceh Province, Indonesia

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d150101 ◽

2015 ◽

Vol 15 (1) ◽

pp. 1-5

Author(s):

WIDYA PINTAKA BAYU PUTRA

Keyword(s):

Growth Hormone ◽

Restriction Enzyme ◽

Cattle Breed ◽

Pcr Rflp ◽

Aceh Province

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SNPs in AKIRIN2, TTN, EDG1 and MYBPC1 genes are associated with growth-related traits in Chinese Qinchuan cattle

10.21203/rs.3.rs-25135/v1 ◽

2020 ◽

Author(s):

Yaxing Li ◽

Gong Cheng ◽

Yunbo Zhao ◽

Chunling Bai ◽

Shenyuan Wang ◽

...

Keyword(s):

Molecular Marker ◽

Cattle Breed ◽

Japanese Black Cattle ◽

Qinchuan Cattle ◽

Korean Cattle ◽

Cattle Breeds ◽

Chinese Cattle ◽

Growth Performances ◽

Pcr Rflp ◽

European Cattle

Abstract Growth performances are the main breeding objectives in Chinese beef cattle. The objective of this study was to confirm genetic effects of the c.*188G > A SNP in AKIRIN2, the g.231054C > T SNP in TTN, the g.1471620G > T SNP in EDG1, and the g.70014208A > G SNP in MYBPC1 gene on growth-related traits in Chinese Qinchuan cattle, as well as to compare the frequencies of the well-characterized alleles of these SNPs among six Chinese cattle populations, three Japanese cattle populations, two European cattle populations and one Korean cattle breed. In this study, a total of 655 cattle samples were genotyped using MassARRAY and PCR-RFLP. Association analysis indicated that the four SNPs have effects one to six indexes of growth-related traits including body length, wither height, hip height, hip width, rump length, chest depth and chest circumference in Chinese Qinchuan cattle (P < 0.05 to P < 0.001). The well-characterized A (c.*188G > A), T (g.231054C > T) and T (g.1471620G > T) alleles in Japanese Black cattle were significantly higher than Chinese cattle breeds, on the contrary, the G allele (g.70014208A > G) was markedly higher in Chinese cattle breeds than other cattle breeds. These results suggest that the four SNPs might be useful as a molecular marker for growth-related traits in Chinese Qinchuan cattle.

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Association of IGFBP-3 gene polymorphism g. 3.930 G>A with birth size and birth weight in crossbred beef cattle

Journal of the Indonesian Tropical Animal Agriculture ◽

10.14710/jitaa.44.4.356-363 ◽

2019 ◽

Vol 44 (4) ◽

pp. 356

Author(s):

T. Hartatik ◽

D. A. Priyadi ◽

P. Panjono ◽

S. Bintara ◽

I. Ismaya ◽

...

Keyword(s):

Birth Weight ◽

Body Length ◽

Cattle Breed ◽

Gene Marker ◽

Birth Size ◽

Potential Marker ◽

Pcr Rflp ◽

Speed Up ◽

The One ◽

Igfbp 3

Crossing between cattle breeds is the one way to improve the genetic quality of cattle. Controlling crosses using genetic markers will speed up the process. The IGFBP-3 gene is a potential marker of selection due to its role in regulating growth and metabolism in cattle. The aim of this study was to determine the effect of genotype on birth size and birth weight using IBFBP-3 gene marker. A total of 32 cattle consisted of 10 Brahman Cross cattle, 14 cattle results of Brahman Cross cow crosses with Belgian Blue bull, and 8 cattle results of Brahman Cross cow crosses with Wagyu bull. Sequences of 563 bp, including the part intron 2, exon 3, and the part intron 3. Genotyping was done by sequencing method and PCR-RFLP method with the restriction enzyme PvuII. An SNP was identified at location g 3,930 (G>A). Two genotypes were observed, namely GA and GG. The genotype did not affect the performance of birth (body weight, body length, height at withers, heart girth) of the research sample. Birth traits are influenced by the cattle breed, it can be seen that Brahman Cross cattle have a body length that is bigger than the other crossbred.

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The The favorable alleles of AKIRIN2:c.*188G>A, EDG1:c.-312A>G and TTN:g.231054C>T as candidate markers for high-marbling are very low in Bali cattle

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d200405 ◽

2019 ◽

Vol 20 (4) ◽

pp. 965-970

Author(s):

SAIFUL ANWAR ◽

ARI SULISTYO WULANDARI ◽

WIDYA PINTAKA BAYU PUTRA ◽

SYAHRUDDIN SAID

Keyword(s):

Genetic Polymorphism ◽

Beef Cattle ◽

Candidate Genes ◽

Cattle Breed ◽

The Other ◽

Favorable Alleles ◽

Factors Influencing ◽

Pcr Rflp ◽

Bali Cattle

Abstract. Anwar S, Wulandari AS, Putra WPB, Said S. 2019. The favorable alleles of AKIRIN2:c.*188G>A, EDG1:c.-312A>G and TTN:g.231054C>T as candidate markers for high-marbling are very low in Bali cattle. Biodiversitas 20: 965-970. Marbling is one of primary factors influencing the palatability of beef. The AKIRIN2, EDG1 and TTN genes are considered as the three candidate genes responsible for high-marbling in beef cattle. This study aimed to investigate the genetic polymorphism of the SNPs of AKIRIN2:c.*188G>A, EDG1:c.-312A>G and TTN g.231054C>T in Bali cattle. A total of 125 DNA samples of Bali cattle were genotyped for AKIRIN2 and TTN gene and 82 DNA samples for EDG1 gene using PCR-RFLP method. In the present study, it was found that the A allele of AKIRIN2:c.*188G>A, the G allele of EDG1:c.-312A>G and the T allele of TTN g.231054C>T that considered as the favorable alleles were found to be very low in Bali cattle (0.004, 0.000, and 0.004, respectively). This results indicated that the polymorphism of AKIRIN2:c.*188G>A, EDG1:c.-312A>G and TTN g.231054C>T were very low and can be considered as monomorphic in Bali cattle. These findings suggested that Bali cattle may not genetically potential as a high-marbling cattle breed. However, the other specific genes for high-marbling still needs to be investigated in Bali cattle.

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