In vitro Activity of Four Common Essential Oil Components against Biofilm-producing Pseudomonas aeruginosa

2011 ◽  
Vol 6 (4) ◽  
pp. 394-401 ◽  
Author(s):  
Saad Ibnsouda K ◽  
Soumya El abed ◽  
Abdellah Houari ◽  
Hassan Latrache ◽  
Adnane Remmal
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Essential Oil ◽  
Vitro Activity ◽  
In Vitro Activity ◽  
Oil Components

Murepavadin antimicrobial activity against and resistance development in cystic fibrosis Pseudomonas aeruginosa isolates

2020 ◽  
Author(s):  
María Díez-Aguilar ◽  
Marta Hernández-García ◽  
María-Isabel Morosini ◽  
Ad Fluit ◽  
Michael M Tunney ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Spontaneous Mutation ◽  
Lung Surfactant ◽  
Vitro Activity ◽  
In Vitro Activity ◽  
Broth Microdilution ◽  
Agar Dilution

Abstract Background Murepavadin, a novel peptidomimetic antibiotic, is being developed as an inhalation therapy for treatment of Pseudomonas aeruginosa respiratory infection in people with cystic fibrosis (CF). It blocks the activity of the LptD protein in P. aeruginosa causing outer membrane alterations. Objectives To determine the in vitro activity of murepavadin against CF P. aeruginosa isolates and to investigate potential mechanisms of resistance. Methods MIC values were determined by both broth microdilution and agar dilution and results compared. The effect of artificial sputum and lung surfactant on in vitro activity was also measured. Spontaneous mutation frequency was estimated. Bactericidal activity was investigated using time–kill assays. Resistant mutants were studied by WGS. Results The murepavadin MIC50 was 0.125 versus 4 mg/L and the MIC90 was 2 versus 32 mg/L by broth microdilution and agar dilution, respectively. Essential agreement was >90% when determining in vitro activity with artificial sputum or lung surfactant. It was bactericidal at a concentration of 32 mg/L against 95.4% of the strains within 1–5 h. Murepavadin MICs were 2–9 two-fold dilutions higher for the mutant derivatives (0.5 to >16 mg/L) than for the parental strains. Second-step mutants were obtained for the PAO mutS reference strain with an 8×MIC increase. WGS showed mutations in genes involved in LPS biosynthesis (lpxL1, lpxL2, bamA2, lptD, lpxT and msbA). Conclusions Murepavadin characteristics, such as its specific activity against P. aeruginosa, its unique mechanism of action and its strong antimicrobial activity, encourage the further clinical evaluation of this drug.

scholarly journals In vitro activity of the essential oil from Hesperozygis myrtoides on Rhipicephalus (Boophilus) microplus and Haemonchus contortus

2017 ◽  
Vol 27 (1) ◽  
pp. 70-76 ◽  
Author(s):  
Caroline V.V. Castilho ◽  
Rafaela R. Fantatto ◽  
Yousmel A. Gaínza ◽  
Humberto R. Bizzo ◽  
Nancy S. Barbi ◽  
...  
Keyword(s):  
Essential Oil ◽  
Haemonchus Contortus ◽  
Boophilus Microplus ◽  
Vitro Activity ◽  
In Vitro Activity

scholarly journals Antiprotozoal Activity of Turkish Origanum onites Essential Oil and Its Components

Molecules ◽  
2019 ◽  
Vol 24 (23) ◽  
pp. 4421 ◽  
Author(s):  
Tasdemir ◽  
Kaiser ◽  
Demirci ◽  
Demirci ◽  
Baser
Keyword(s):  
Gas Chromatography ◽  
Essential Oil ◽  
Mammalian Cells ◽  
Leishmania Donovani ◽  
Vitro Activity ◽  
In Vitro Activity ◽  
Flame Ionization ◽  
Main Component ◽  
Origanum Onites

Essential oil of Origanum species is well known for antimicrobial activity, but only a few have been evaluated in narrow spectrum antiprotozoal assays. Herein, we assessed the antiprotozoal potential of Turkish Origanum onites L. oil and its major constituents against a panel of parasitic protozoa. The essential oil was obtained by hydrodistillation from the dried herbal parts of O. onites and analyzed by Gas Chromatography-Flame Ionization Detector (GC-FID) and Gas Chromatography coupled with Mass Spectrometry (GC-MS). The in vitro activity of the oil and its major components were evaluated against Trypanosoma brucei rhodesiense, T. cruzi, Leishmania donovani, and Plasmodium falciparum. The main component of the oil was identified as carvacrol (70.6%), followed by linalool (9.7%), p-cymene (7%), γ-terpinene (2.1%), and thymol (1.8%). The oil showed significant in vitro activity against T. b. rhodesiense (IC50 180 ng/mL), and moderate antileishmanial and antiplasmodial effects, without toxicity to mammalian cells. Carvacrol, thymol, and 10 additional abundant oil constituents were tested against the same panel; carvacrol and thymol retained the oil’s in vitro antiparasitic potency. In the T. b. brucei mouse model, thymol, but not carvacrol, extended the mean survival of animals. This study indicates the potential of the essential oil of O. onites and its constituents in the treatment of protozoal infections.

scholarly journals Anti-Legionella Activity of Essential Oil of Satureja cuneifolia

2014 ◽  
Vol 9 (5) ◽  
pp. 1934578X1400900
Author(s):  
Valerija Dunkić ◽  
Antonija Mikrut ◽  
Nada Bezić
Keyword(s):  
Phenolic Compounds ◽  
Essential Oil ◽  
Vitro Activity ◽  
In Vitro Activity ◽  
High Concentration ◽  
Different Sources

The essential oil of Satureja cuneifolia Ten. was characterized by a high concentration of the phenolic compounds carvacrol (21.3%) and thymol (9.2%). The in vitro activity of the essential oil against Legionela pneumophila serogroups (SG) 1 and 2-15 and Legionella spp. from different sources, using micro-dilution, showed that L. pneumofila is sensitive to the oil, with MICs ranging from 0.12 to 0.5%, v/v, and a MBC at 0.5 to 1%, v/v. The essential oil of S. cuneifolia was effective in the reduction of Legionellosis infections.

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