Molecular Study of Salmonella enteritidis in Poultry Samples by PCR, Plasmid Curing, Antibiotic Resistance and Protein Pattern Analysis

2007 ◽  
Vol 10 (10) ◽  
pp. 1562-1570 ◽  
Author(s):  
M.H. Mirmomeni ◽  
A. Hosseinzadeh Colagar ◽  
S. Ghazaey
Keyword(s):  
Antibiotic Resistance ◽  
Salmonella Enteritidis ◽  
Pattern Analysis ◽  
Protein Pattern ◽  
Molecular Study ◽  
Plasmid Curing

scholarly journals Application of physico-chemical typing methods for the epidemiological analysis ofSalmonella enteritidisstrains of phage type 25/17

1994 ◽  
Vol 113 (3) ◽  
pp. 411-424 ◽  
Author(s):  
G. Seltmann ◽  
W. Voigt ◽  
W. Beer
Keyword(s):  
Salmonella Enteritidis ◽  
Pattern Analysis ◽  
Protein Pattern ◽  
Phage Type ◽  
Cell Protein ◽  
Enzyme Electrophoresis ◽  
General Validity ◽  
Physico Chemical ◽  
Typing Methods ◽  
Ft Ir

SUMMARYEighty-nineSalmonella enteritidisphage type 25/17 strains isolated from a localized outbreak in the German state Nordrhein-Westfalen (outbreak NWI) could not be further differentiated by biochemotyping and plasmid pattern analysis. They were submitted to a complex typing system consisting of modern physico-chemical analytical procedures. In lipopolysaccharide pattern analysis the strains proved to be homogeneous. In multilocus enzyme electrophoresis, outer membrane and whole cell protein pattern (WCPP) analysis, and Fourier transform infrared (FT–IR) spectroscopy (increasing extent of differentiation in the given order) strains deviating from each basal pattern were found. The extent of correspondence in these deviations was satisfactory.Forty-six strains of the same sero- and phage type, however, obtained from different outbreaks, were additionally typed. The results obtained with them indicate that the data of the first group were not restricted to strains from outbreak NWI, but of general validity.It was found that both WCPP and FT–IR represent valuable methods for the sub-grouping of bacteria.

scholarly journals Antibiotic resistance in Salmonella Enteritidis isolated from broiler carcasses

2006 ◽  
Vol 37 (3) ◽  
pp. 368-371 ◽  
Author(s):  
Martha Oliveira Cardoso ◽  
Aldemir Reginato Ribeiro ◽  
Luciana Ruschel dos Santos ◽  
Fernando Pilotto ◽  
Hamilton L.S. de Moraes ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Salmonella Enteritidis

scholarly journals EVALUATION OF THE IMPACT OF PLASMID CURING ON ANTIBIOTIC RESISTANCE ON SOME CLINICAL ISOLATES OF ESCHERICHIA COLI

2020 ◽  
Vol 4 (3) ◽  
pp. 323-327
Author(s):  
Mamunu Abdulkadir SULAIMAN ◽  
H.S Muhammad ◽  
Aliyu Muhammad Sani ◽  
Aminu Ibrahim ◽  
Ibrahim Muhammad Hussain ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Clinical Isolates ◽  
Diffusion Method ◽  
Plasmid Curing ◽  
Sensitivity Testing ◽  
E Coli ◽  
Risk Of Death ◽  
Mar Index ◽  
The Impact

Multidrug resistance (MDR) exhibited by some strains of Escherichia coli may be due to acquiring mobile genetic element (R-plasmid) by the bacteria, or intrinsically induced by inappropriate use of antibiotics by the hosts.  Infection by such strains may result to prolonged illness and greater risk of death. The study evaluated the impact of curing on antibiotic resistance on selected clinical isolates of E. coli. Twenty clinical isolates of E. coli from our previous studies were re-characterized using conventional microbiological techniques. Antibiotic sensitivity testing was determined by disk diffusion method, MDR selected based on resistance to ≥ 2 classes of antibiotics. Multiple antibiotic resistance (MAR) index was determined as ratio of the number of antibiotic resisted to the total number of antibiotics tested and considered significant if ≥. 0.2. The isolates that showed significant MAR index were subjected to plasmid curing using acridine orange, thereafter, profiled for plasmid and the cured ones were re-tested against the antibiotics they initially resisted. Out of the 20 isolates, 19 (95%) were confirmed as E. coli, all (100%) of which were MDRs, which was highest against augmentin (78.9%) followed by amoxacillin (52.6%). However, after the plasmid curing only 6 (31.6%) out of the 19 isolates cured retained significant MAR index and the level of the significance had reduced drastically in 16 (84.2%) isolates. Conclusively, curing assay can completely eliminate R-plasmid acquired resistance. More studied on plasmid curing agents for possible augmentation of the agents into antibiotics may see the rise of successful antibiotic era again.

scholarly journals Genotypic characterization of antibiotic-resistant Salmonella Enteritidis isolates in Dakar, Senegal

2007 ◽  
Vol 1 (03) ◽  
pp. 284-288 ◽  
Author(s):  
Amy Gassama Sow ◽  
Abdoul Aziz Wane ◽  
Mamadou Hadi Diallo ◽  
Cheikh Saad-Bouh Boye ◽  
Awa Aïdara-Kane
Keyword(s):  
Antibiotic Resistance ◽  
Antimicrobial Resistance ◽  
Salmonella Enterica ◽  
Salmonella Enteritidis ◽  
Data Bank ◽  
Human Infection ◽  
Molecular Techniques ◽  
Conjugative Plasmid ◽  
Bacterial Strains ◽  
Salmonella Enterica Serovar Enteritidis

Background: It is well established that Salmonella enterica is a major cause of food-borne disease worldwide. In Africa, according to the Who Global Salm-Surv country data bank from 2000 to 2002 Salmonella enterica serovar Enteritidis was the most common serotype involved in human salmonellosis. In Dakar this serotype of Salmonella has been reported as a frequent and an increasing cause of human infection. Methodology: The genetic determinants of the antimicrobial resistance of 25 selected multiresistant strains of Salmonella enterica serovar Enteritidis referred to the National Reference Center for Enterobacteria (NRCE) in Dakar were investigated using molecular techniques. Results: All strains carried blaTEM 1 genes. Five harboured three types of class 1 integrons with gene cassettes dfrA15, dfrA1-aadA1 and dfrA7. Multiresistance was due to a 23 Kb conjugative plasmid. DNA fingerprinting by macrorestriction of genomic DNA revealed a single related group suggesting that strains might be clonal. Conclusions: The spread of resistance genes through plasmid transfer plays an important role in the dissemination of antibiotic resistance in enteric pathogens such as Salmonella Enteritidis; the risk of transmissibility of antibiotic resistance between different bacterial strains highlights the urgent need to develop strategies to limit the spread of antimicrobial resistance among bacterial enteropathogens.

Multiplication in Egg Yolk and Survival in Egg Albumen of Genetically and Phenotypically Characterized Salmonella Enteritidis Strains

2018 ◽  
Vol 81 (6) ◽  
pp. 876-880 ◽  
Author(s):  
RICHARD K. GAST ◽  
JEAN GUARD ◽  
RUPA GURAYA ◽  
AUDE LOCATELLI
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Gene ◽  
Salmonella Enteritidis ◽  
Antibiotic Resistance Gene ◽  
Genetic Selection ◽  
Egg Yolk ◽  
Phenotypic Properties ◽  
C Storage ◽  
Egg Albumen ◽  
Human Illness

ABSTRACT Prompt refrigeration of eggs to prevent the multiplication of Salmonella Enteritidis to high levels during storage is an important practice for reducing the risk of egg-transmitted human illness. The efficacy of egg refrigeration for achieving this goal depends on the interaction among the location of contamination, the ability of contaminant strains to survive or multiply, and the rate at which growth-restricting temperatures are attained. The present study assessed the significance of several characterized genetic and phenotypic properties for the capabilities of 10 Salmonella Enteritidis isolates to multiply rapidly in egg yolk and survive for several days in egg albumen during unrefrigerated (25°C) storage. The growth of small numbers of each Salmonella Enteritidis strain (approximately 101 CFU/mL) inoculated into egg yolk samples was determined after 6 and 24 h of incubation. The survival of larger numbers of Salmonella Enteritidis (approximately 105 CFU/mL) inoculated into albumen samples was determined at 24 and 96 h of incubation. In yolk, the inoculated Salmonella Enteritidis strains multiplied to mean levels of approximately 102.6 CFU/mL after 6 h of incubation and 108.3 CFU/mL after 24 h. In albumen, mean levels of approximately 104.6 CFU/mL Salmonella Enteritidis were maintained through 96 h. The concentrations of the various Salmonella strains after incubation in either yolk or albumen were distributed over relatively narrow ranges of values. Significant (P < 0.01) differences observed among individual strains suggested that maintenance of the fimbrial gene sefD may have positive genetic selection value by improving fitness to grow inside egg yolk, whereas the antibiotic resistance gene blaTEM-1 tet(A) appeared to have negative genetic selection value by decreasing fitness to survive in egg albumen.

Antibiotic Resistance of Salmonella spp. and Listeria spp. Isolates from Traditionally Made Fresh Sausages in Greece

1998 ◽  
Vol 61 (10) ◽  
pp. 1378-1380 ◽  
Author(s):  
AMIN ABRAHIM ◽  
ANNA PAPA ◽  
NIKOLAOS SOULTOS ◽  
IOANNIS AMBROSIADIS ◽  
ANTONIS ANTONIADIS
Keyword(s):  
Antibiotic Resistance ◽  
Listeria Monocytogenes ◽  
Salmonella Typhimurium ◽  
Salmonella Enteritidis ◽  
Antibacterial Agents ◽  
Listeria Innocua ◽  
Salmonella Spp ◽  
Listeria Welshimeri ◽  
Butcher Shops

Sixty-five samples of traditionally made fresh sausages obtained from retail shops and butcher shops in northem Greece were screened for the presence of Salmonella spp. and Listeria spp. Salmonella spp. were found in 20% of the samples tested (54% Salmonella typhimurium and 46% Salmonella enteritidis). The prevalence of Listeria spp. in the samples was 26% (12% Listeria monocytogenes, 76% Listeria innocua, and 12% Listeria welshimeri). Nine of 13 Salmonella isolates were found to be resistant to ampicillin and 4 of 13 showed intermediate sensitivity; 1 of 13 was found to be resistant to chloramphenicol and 1 of 13 to tetracycline. Two strains of Salmonella typhimurum were multiresistant (resistant to ampicillin, chloramphenicol, and norfloxacin). All Listeria isolates were sensitive to the antibacterial agents tested that are commonly used for the treatment of human listeriosis.

scholarly journals Prevalence and antibiotic resistance of Salmonella Enteritidis and Salmonella Typhimurium in raw chicken meat at retail markets in Malaysia

2016 ◽  
Vol 95 (8) ◽  
pp. 1888-1893 ◽  
Author(s):  
T.Y. Thung ◽  
N.A. Mahyudin ◽  
D.F. Basri ◽  
C.W.J. Wan Mohamed Radzi ◽  
Y. Nakaguchi ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Salmonella Typhimurium ◽  
Salmonella Enteritidis ◽  
Chicken Meat ◽  
Retail Markets
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