Evaluation of the Antimicrobial Activity of Zanthoxylum zanthoxyloides Root Bark Extracts

A fungus, isolated from the root bark of Melia azedarach (Meliaceae), from which a series of meroterpenes have been reported, was identified as Penicillium brasilianum based on analysis of the ITS region of ribosomal DNA. From a rice culture of this fungus, the known phenylpropanoid amides brasiliamide A and B were obtained together with and a new, slightly modified congener, along with the meroterpenoids preaustinoid A1, preaustinoid B2 and austinolide. The compounds were isolated by the use of combined chromatographic procedures and identified by physical methods, mainly 1D and 2D NMR experiments, with distinction for 1H{15N} HMBC applied to brasiliamide A. The amides were tested for their antimicrobial activity and showed only weak inhibitory effects, against a set of pathogenic bacteria

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Toxicological evaluation of Zanthoxylum zanthoxyloides (Lam) Zepernick Timler root bark used as biopesticide and medicine

Journal of Medicinal Plants Research ◽

10.5897/jmpr2020.7081 ◽

2021 ◽

Vol 15 (2) ◽

pp. 108-117

Author(s):

Gbate Mohammed ◽

Michael Ashamo Olufemi ◽

Lawrence Kayode Akinwande

Keyword(s):

Root Bark ◽

Toxicological Evaluation ◽

Zanthoxylum Zanthoxyloides

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Activity against Streptococcus Pneumoniae of the Essential Oil and 5-(3-Buten-1-ynyl)-2, 2′-bithienyl Isolated from Chrysactinia mexicana Roots

Natural Product Communications ◽

10.1177/1934578x1100600728 ◽

2011 ◽

Vol 6 (7) ◽

pp. 1934578X1100600

Author(s):

Bárbara Missiam Mezari Guevara Campos ◽

Anabel Torres Cirio ◽

Verónica Mayela Rivas Galindo ◽

Ricardo Salazar Aranda ◽

Noemi Waksman de Torres ◽

...

Keyword(s):

Antimicrobial Activity ◽

Streptococcus Pneumoniae ◽

Essential Oil ◽

Gel Chromatography ◽

Root Bark ◽

Reverse Phase ◽

Mass Detector ◽

Isolated Compound ◽

Silica Gel Chromatography

The essential oil of Chrysactinia mexicana retrieved from the root bark was characterized by gas chromatography coupled to a mass detector. The compounds silphiperfol-5-ene, 7-epi- silphiperfol-5-ene, modheph-2-ene, α-isocomene, β-isocomene and methyl-linoleate were identified. The principal compound (76.42%) could not be identified by the library and was further isolated through a reverse phase C-18 chromatography followed by silica gel chromatography and identified as 5-(3-buten-1-ynyl)-2,2′-bithienyl. Both the oil and the isolated compound were tested for their antimicrobial activity against two strains of Streptococcus pneumoniae resistant to β-lactam antibiotics. MICs were 250 μg/mL and 125 μg/mL respectively. This is the first report about extraction of oil and compound 5-(3-buten-1-ynyl)-2, 2′-bithienyl from roots of Chrysactinia mexicana as well as the determination of antimicrobial activity against S. pneumoniae.

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Anti-plasmodial Effects of Zanthoxylum zanthoxyloides

Planta Medica ◽

10.1055/a-0973-0067 ◽

2019 ◽

Vol 85 (13) ◽

pp. 1073-1079 ◽

Cited By ~ 1

Author(s):

Christopher Dean Goodman ◽

An Thuy Hoang ◽

Drissa Diallo ◽

Karl Egil Malterud ◽

Geoffrey I. McFadden ◽

...

Keyword(s):

Brine Shrimp ◽

Methanol Extract ◽

Stem Bark ◽

Artemia Salina ◽

Root Bark ◽

Ic50 Values ◽

Zanthoxylum Zanthoxyloides ◽

Parasitic Activity ◽

Resistant Strains

Abstract Zanthoxylum zanthoxyloides, syn. Fagara zanthoxyloides, is a tree growing in West Africa and is used in traditional medicine against a variety of diseases, including malaria. In the work reported here, root bark and stem bark extracts of this tree, as well as compounds isolated from the extracts, have been investigated for activity in vitro against chloroquine-sensitive and chloroquine-resistant strains of Plasmodium falciparum. In addition, toxicity against nauplii of the brine shrimp Artemia salina has been studied. Dichloromethane extracts of the root bark and stem bark, and a methanol extract of the stem bark, showed anti-parasitic activity towards chloroquine-sensitive as well as chloroquine-resistant P. falciparum, with IC50 values between 1 and 10 µg/mL. Among the isolated compounds, bis-dihydrochelerythrinyl ether, buesgenine, chelerythrine, γ-fagarine, skimmianine, and pellitorine were the most active, with IC50 values of less than 5 µg/mL. The dichloromethane extracts were toxic to brine shrimp nauplii, with LC50 values of less than 1 µg/mL. Methanol extracts were much less toxic (LC50 between 50 and 100 µg/mL). Among the isolated substances, bis-dihydrochelethrinyl ether was the most toxic (LC50 ca. 2 µg/mL).

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Herbal mouthwash formulated with the leaf extract of Jatropha gossypiifolia Linn. (Euphorbiaceae) exhibited in vitro antimicrobial activity against selected oral pathogens

Journal of Pharmacy & Bioresources ◽

10.4314/jpb.v18i3.5 ◽

2021 ◽

Vol 18 (3) ◽

pp. 207-214

Author(s):

Abel Olusola Idowu ◽

Nwamaka Henrietta Igbokwe ◽

Oreoluwa Ayomide Abiodun ◽

Chijioke Ofomata

Keyword(s):

Antimicrobial Activity ◽

Leaf Extract ◽

Antimicrobial Activities ◽

Oral Disease ◽

Bioactive Metabolites ◽

Diffusion Method ◽

Klebsiella Pneumonia ◽

Root Bark ◽

Oral Pathogens ◽

Jatropha Gossypiifolia

Increase in the incidence of oral disease, resistance to antibiotics and adverse effect to synthetic medicines has made search for alternative safe, effective and cheaper treatment options imperative, preferably from plant sources. This study evaluated the antimicrobial profile of ethanol extracts of leaf and root bark of Jatropha gossypiifolia and mouthwash formulation containing the leaf extract. Extraction of plant parts was done by cold maceration with 70% ethanol. The antimicrobial activities of the extracts and the formulated mouthwash were evaluated againstStaphylococcus aureus, Escherichia coli, Klebsiella pneumonia, Bacillus subtilis and Candida albicans using Agar well diffusion method. The minimum inhibitory concentration of the extracts on susceptible organisms was determined according to the Clinical Laboratory Standards Institute protocol. The physicochemical parameters of the formulated mouthwash were assessed using standard methods. J. gossypiifolia leaf and root bark extracts exhibited a concentration dependent antimicrobial activity and MIC ranging from 50 to 260 mg/ml against test organisms. The formulated herbal mouthwash showed effective antimicrobial activity and suitable cosmetics properties. This study indicates that the leaf extracts of J. gossypiifolia possess bioactive metabolites with strong antimicrobial activities and its herbal mouthwash formulation has potential in the treatment of various diseases caused by oral pathogens.

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