Activity against Streptococcus Pneumoniae of the Essential Oil and 5-(3-Buten-1-ynyl)-2, 2′-bithienyl Isolated from Chrysactinia mexicana Roots

The essential oil of Chrysactinia mexicana retrieved from the root bark was characterized by gas chromatography coupled to a mass detector. The compounds silphiperfol-5-ene, 7-epi- silphiperfol-5-ene, modheph-2-ene, α-isocomene, β-isocomene and methyl-linoleate were identified. The principal compound (76.42%) could not be identified by the library and was further isolated through a reverse phase C-18 chromatography followed by silica gel chromatography and identified as 5-(3-buten-1-ynyl)-2,2′-bithienyl. Both the oil and the isolated compound were tested for their antimicrobial activity against two strains of Streptococcus pneumoniae resistant to β-lactam antibiotics. MICs were 250 μg/mL and 125 μg/mL respectively. This is the first report about extraction of oil and compound 5-(3-buten-1-ynyl)-2, 2′-bithienyl from roots of Chrysactinia mexicana as well as the determination of antimicrobial activity against S. pneumoniae.

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Antimicrobial Activity and Synergic Effect of Cinnamomum burmannii’s Essential Oil & its isolated compound (Cinnamaldehyde)

International Conference on Chemical, Agricultural and Medical Sciences (CAMS-2013) Dec. 29-30, 2013 Kuala Lumpur (Malaysia) ◽

10.15242/iicbe.c1213073 ◽

2013 ◽

Keyword(s):

Antimicrobial Activity ◽

Essential Oil ◽

Synergic Effect ◽

Isolated Compound

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Gas-Liquid Chromatographic Determination of Oxydemeton-Methyl in Commercial Formulations

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/61.3.500 ◽

1978 ◽

Vol 61 (3) ◽

pp. 500-503

Author(s):

Leonard R Schronk ◽

Billy M Colvin ◽

Alan R Hanks

Keyword(s):

Rapid Determination ◽

Internal Standard ◽

Chromatographic Determination ◽

Peak Height ◽

Detector Response ◽

Gel Chromatography ◽

Gas Liquid Chromatography ◽

Flame Ionization ◽

Silica Gel Chromatography

Abstract Flame ionization gas-liquid chromatography (GLC) with a 10% DC-200 on 80-100 mesh Gas-Chrom Q column is used for the rapid determination of oxydemeton-methyl in commercial formulations. The detector response is linear for 1.0–10.0 μg oxydemeton-methyl, with a sensitivity of 4 ng. The column was stabilized before analysis by injection of a lecithin solution. Samples were diluted with chloroform and injected; peak height ratios were used for quantitation with fluoranthrene as the internal standard. Carbaryl was removed from mixed formulations by silica gel chromatography, oxydemeton- methyl was eluted with methanolchloroform (2+98), and the eluate was collected for infrared (IR) and GLC analyses. Methoxychlor and Karathane were removed by chromatography on Florisil before IR analysis. GLC results compare favorably with those for IR, with recoveries ranging from 98.44 to 102.88% for spiked formulations.

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Determination of Phospholipid Fractions in Red Blood Cells in Retarded Physically Handicapped Children

Annals of Clinical Biochemistry International Journal of Laboratory Medicine ◽

10.1177/000456327301000104 ◽

1973 ◽

Vol 10 (1-6) ◽

pp. 23-27 ◽

Cited By ~ 1

Author(s):

Fay M. John ◽

G. C. Forrest

Keyword(s):

Red Blood Cells ◽

Silica Gel ◽

Blood Cells ◽

Red Cells ◽

Gel Chromatography ◽

Handicapped Children ◽

Silica Gel Chromatography ◽

Physically Handicapped

Phospholipids from the red cells of 85 children, both normal and belonging to various clinical groups, have been extracted and subjected to silica gel chromatography. The several phospholipids have been quantitated. The results have not proved to be useful in diagnosis and hence do not confirm earlier reports. The problems associated with analysis of phospholipids by this type of method are discussed.

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Determination of Organochlorine, Organophosphorus, and Triazine Pesticide Residues in Wine by Gas Chromatography with Electron Capture and Nitrogen–Phosphorus Detection

Journal of AOAC International ◽

10.1093/jaoac/79.6.1423 ◽

1996 ◽

Vol 79 (6) ◽

pp. 1423-1427 ◽

Cited By ~ 8

Author(s):

Rosario Garcia-Repetto ◽

Isabel Garrtoo ◽

Manuel Repetto

Keyword(s):

Gas Chromatography ◽

Electron Capture ◽

Pesticide Residues ◽

Solid Phase ◽

Electron Capture Detection ◽

Gel Chromatography ◽

Phase Extraction ◽

Silica Gel Chromatography ◽

Nitrogen Phosphorus

Abstract A multiresidue analytical method is described for determining 6 pesticides in wine: chlorpyrifos, dimethoate, quinalphos, simazine, tetradifon, and endosulfan. Wine (200 mL) is extracted with dichloromethane (50 mL, 3 times), and the evaporated extracts are purified by silica gel chromatography. Pesticide residues are eluted with n-hexane, n-hexane-benzene (1 + 1), benzene, benzene–acetone (1 + 1), and acetone. Residues are determined by gas chromatography with nitrogen–phosphorus and electron capture detection, using CPSH-5CB, Carbo wax-20M, and CPSH-19CB columns. Recoveries of pesticides added at 0.025 μg/L were 83–97%, with standard deviations of 0.01–0.05%. Detection limits were 0.13–8.9 ng/L, except for simazine (36.7 μg/L). Results are compared with those obtained with a solid-phase extraction (C18) purification and with different eluant series.

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Development and Validation of an HPLC-MS/MS Method for Rapid Simultaneous Determination of Cefprozil Diastereomers in Human Plasma

International Journal of Analytical Chemistry ◽

10.1155/2018/6959761 ◽

2018 ◽

Vol 2018 ◽

pp. 1-9 ◽

Cited By ~ 3

Author(s):

Guodong He ◽

Liping Mai ◽

Xipei Wang

Keyword(s):

Mass Spectrometry ◽

Antimicrobial Activity ◽

Multiple Reaction Monitoring ◽

Internal Standard ◽

Reaction Monitoring ◽

Reverse Phase ◽

Development And Validation ◽

Positive Ion ◽

Sensitive Method

Background. Both cis- and trans-cefprozil have antimicrobial activity, but their potencies are quite different. It is therefore necessary to develop a sensitive method to simultaneously determine both isomers for pharmacokinetic and bioequivalence studies. Methods. An LC-MS/MS method, using stable isotope-labeled cefprozil as the internal standard, was developed and validated. The analytes were extracted from plasma by protein precipitation and separated on a reverse-phase C18 column using a gradient program consisting of 0.5% formic acid and acetonitrile within 4 min. The mass spectrometry acquisition was performed with multiple reaction monitoring in positive ion mode using the respective [M+H]+ ions, m/z 391.2→114.0 for cefprozil and 395.0→114.5 for cefprozil-D4. Results. The calibration curves were linear over the ranges of 0.025–15 μg/mL for cis-cefprozil and 0.014–1.67 μg/mL for trans-cefprozil. The accuracies for the cis and trans isomers of cefprozil were 93.1% and 103.0%, respectively. The intra- and interassay precisions for the QC samples of the isomers were < 14.3%. The intra- and interassay precisions at the LLOQ were < 16.5%. Conclusions. The method was sensitive and reproducible and was applied in a pilot pharmacokinetic study of healthy volunteers.

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Gas-Liquid Chromatographic Determination of Captan and Two Metabolites in Milk and Meat

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/60.3.679 ◽

1977 ◽

Vol 60 (3) ◽

pp. 679-681

Author(s):

John H Onley

Keyword(s):

Ethyl Acetate ◽

Silica Gel ◽

Chromatographic Determination ◽

Gel Chromatography ◽

Milk Samples ◽

Liquid Chromatographic Determination ◽

Meat Samples ◽

Silica Gel Chromatography ◽

Liquid Chromatographic

Abstract A gas-liquid chromatographic (GLC) method has been developed for the determination of captan (N-trichloromethylthio-4-cyclohexene-1, 2-dicarboximide) and 2 metabolites, tetrahydrophthalimide (THPI) and tetrahydrophthalamic acid (THPMA), in milk and meat. The sample is extracted with ethyl acetate and is cleaned up by acetonitrile partition and silica gel chromatography where captan, THPI, and THPMA are separated. Captan is directly determined by GLC. THPI and THPMA are separately derivatized in an acetone solution of penta fluorobenzyl bromide. The resultant derivatives are purified separately on an Al2O3, column and quantitated by GLC, using an electron capture detector. Recoveries from milk samples fortified at 0.02–10 ppm ranged from 71 to 102%; recoveries from meat samples fortified at 0.04–10 ppm ranged from 75 to 99%.

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GC-MS analysis and antimicrobial activity determination of Citrus medica L. var proper leaf essential oil from South Sulawesi against skin pathogen microorganism

IOP Conference Series Materials Science and Engineering ◽

10.1088/1757-899x/259/1/012001 ◽

2017 ◽

Vol 259 ◽

pp. 012001

Author(s):

Aliyah ◽

A Himawan ◽

H Rante ◽

Mufidah ◽

D R Ningsih

Keyword(s):

Antimicrobial Activity ◽

Essential Oil ◽

Activity Determination ◽

South Sulawesi ◽

Ms Analysis ◽

Citrus Medica ◽

Leaf Essential Oil ◽

Skin Pathogen

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Chemical Composition of Blumea lacera Essential Oil from Nepal. Biological Activities of the Essential Oil and (Z)-Lachnophyllum Ester

Natural Product Communications ◽

10.1177/1934578x1501001028 ◽

2015 ◽

Vol 10 (10) ◽

pp. 1934578X1501001 ◽

Cited By ~ 2

Author(s):

Prabodh Satyal ◽

Bhuwan K. Chhetri ◽

Noura S. Dosoky ◽

Samon Shrestha ◽

Ambika Poudel ◽

...

Keyword(s):

Essential Oil ◽

Biological Activities ◽

Human Tumor ◽

Gas Chromatography Mass Spectrometry ◽

Gel Chromatography ◽

Aerial Parts ◽

Silica Gel Chromatography ◽

Antibacterial And Antifungal ◽

Blumea Lacera ◽

Mcf 7

The essential oil from the aerial parts of Blumea lacera collected from Biratnagar, Nepal, has been obtained by hydrodistillation and analyzed by gas chromatography-mass spectrometry. The major component from the oil, (Z)-lachnophyllum ester, was isolated by preparative silica gel chromatography. B. lacera oil was dominated by (Z)-lachnophyllum ester (25.5%), (Z)-lachnophyllic acid (17.0%), germacrene D (11.0%), (E)-β-farnesene (10.1%), bicyclogermacrene (5.2%), (E)-caryophyllene (4.8%), and (E)-nerolidol (4.2%). Also detected in the oil were (E)-lachnophyllic acid (3.3%) and (E)-lachnophyllum ester (1.7%). (Z)-Lachnophyllum ester exhibited cytotoxic activity against MDA-MD-231, MCF-7, and 5637 human tumor cells, as well as antibacterial and antifungal activity.

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Siderophore and indolic acid production by Paenibacillus triticisoli BJ-18 and their plant growth-promoting and antimicrobe abilities

PeerJ ◽

10.7717/peerj.9403 ◽

2020 ◽

Vol 8 ◽

pp. e9403

Author(s):

Yunzhi Zhang ◽

Jinwei Ren ◽

Wenzhao Wang ◽

Baosong Chen ◽

Erwei Li ◽

...

Keyword(s):

Antimicrobial Activity ◽

Plant Growth ◽

High Performance ◽

Growth Promotion ◽

Bioactive Molecules ◽

Gel Chromatography ◽

Plant Growth Promoting ◽

Luotonin A ◽

Promote Plant Growth ◽

Silica Gel Chromatography

Paenibacillus triticisoli BJ-18, a N2-fixing bacterium, is able to promote plant growth, but the secondary metabolites that may play a role in promoting plant growth have never been characterized. In this study, untargeted metabolomics profiling of P. triticisoli BJ-18 indicated the existence of 101 known compounds, including N2-acetyl ornithine, which is the precursor of siderophores, plant growth regulators such as trehalose 6-phosphate, betaine and trigonelline, and other bioactive molecules such as oxymatrine, diosmetin, luotonin A, (-)-caryophyllene oxide and tetrahydrocurcumin. In addition, six compounds were also isolated from P. triticisoli BJ-18 using a combination of silica gel chromatography, sephadex LH-20, octadecyl silane (ODS), and high-performance liquid chromatography (HPLC). The compound structures were further analyzed by Nuclear Magnetic Resonance (NMR), Mass Spectrometry (MS), and Electronic Circular Dichroism (ECD). The six compounds included three classical siderophore fusarinines identified as deshydroxylferritriacetylfusigen, desferritriacetylfusigen, and triacetylfusigen, and three indolic acids identified as paenibacillic acid A, 3-indoleacetic acid (IAA), and 3-indolepropionic acid (IPA). Both deshydroxylferritriacetylfusigen and paenibacillic acid A have new structures. Fusarinines, which normally occur in fungi, were isolated from bacterium for the first time in this study. Both siderophores (compounds 1 and 2) showed antimicrobial activity against Escherichia coli, Staphylococcus aureus and Bacillus subtilis, but did not show obvious inhibitory activity against yeast Candida albicans, whereas triacetylfusigen (compound 3) showed no antibiosis activity against these test microorganisms. Paenibacillic acid A, IAA, and IPA were shown to promote the growth of plant shoots and roots, and paenibacillic acid A also showed antimicrobial activity against S. aureus. Our study demonstrates that siderophores and indolic acids may play an important role in plant growth promotion by P. triticisoli BJ-18.

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